Strain Name:

B6;129S4-Hexbtm1Rlp/J

Stock Number:

002914

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Richard L Proia,   National Institutes of Health

Appearance
white-bellied agouti
Related Genotype: Aw/?

black
Related Genotype: a/a

Description
Mice homozygous for the Hexbtm1Rlp targeted mutation develop motor defects beginning at about 3 months of age. The defects progressively worsen and homozygous mice die by 4.5 months of age. Mice display gangliosidosis; mice abnormally accumulate GM2 and GA2 ganglioside and serve as a model of Sandhoff disease.

Control Information

  Control
   Wild-type from the colony
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Sandhoff Disease
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Hexbtm1Rlp/Hexbtm1Rlp

        involves: 129S4/SvJae * C57BL/6
  • mortality/aging
  • premature death
    • died around 120 to 150 days of age   (MGI Ref ID J:53080)
    • average life span of 127 days   (MGI Ref ID J:190450)
  • behavior/neurological phenotype
  • abnormal motor capabilities/coordination/movement
    • beginning at 3 months and progressing with age   (MGI Ref ID J:29268)
    • abnormal gait
      • spastic movements of hind limbs starting around 5 months   (MGI Ref ID J:29268)
      • near lack of hind limb movement by 5 months and fore limbs also afflicted   (MGI Ref ID J:29268)
    • bradykinesia
      • by 4 - 5 months of age   (MGI Ref ID J:190450)
    • impaired coordination
      • performance in a rotarod test is impaired at 9 weeks and declines very rapidly after 11 weeks of age   (MGI Ref ID J:29268)
      • at 16 weeks of age, homozygous mice are unable to stay on a rotarod   (MGI Ref ID J:29268)
    • impaired righting response
      • progressive loss of righting response with age   (MGI Ref ID J:53080)
    • tremors
      • by 4 - 5 months of age   (MGI Ref ID J:190450)
  • nervous system phenotype
  • abnormal neuron morphology
    • neuronal storage vacuoles (PAS positive) found throughout the central nervous system   (MGI Ref ID J:29268)
    • vacuoles very abundant in anterior horn motor neurons of the spinal cord   (MGI Ref ID J:29268)
    • PAS positive granules also found in neuropil and dendrites   (MGI Ref ID J:29268)
    • no granules found in cerebral and cerebellar white matter, nerve fiber tracts, spinal roots or optic nerves   (MGI Ref ID J:29268)
  • muscle phenotype
  • muscular atrophy
    • at 5 months of age   (MGI Ref ID J:29268)
  • liver/biliary system phenotype
  • abnormal liver morphology
    • storage vacuoles found in cells in hepatic sinusoids   (MGI Ref ID J:29268)
    • abnormal hepatocyte morphology
      • PAS positive storage vacuoles found   (MGI Ref ID J:29268)
  • renal/urinary system phenotype
  • abnormal proximal convoluted tubule morphology
    • storage vacuoles found in epithelial cells of the proximal tubules   (MGI Ref ID J:29268)
  • growth/size/body phenotype
  • weight loss
    • by 4 - 5 months of age   (MGI Ref ID J:190450)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hexbtm1Rlp related

Metabolism Research

Neurobiology Research
Metabolic Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Hexbtm1Rlp
Allele Name targeted mutation 1, Richard L Proia
Allele Type Targeted (Null/Knockout)
Common Name(s) Hexb-; Sandhoff hexb-;
Mutation Made By Richard Proia,   National Institutes of Health
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Hexb, hexosaminidase B
Chromosome 13
Gene Common Name(s) ENC-1AS; HEL-248;
Molecular Note A neomycin resistance cassette was inserted into and disrupted exon 13 of the gene. This mutation resulted in the production of no detectable functional protein. [MGI Ref ID J:29268]

Genotyping

Genotyping Information

Genotyping Protocols

Hexbtm1Rlp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Hexbtm1Rlp related

Boland B; Smith DA; Mooney D; Jung SS; Walsh DM; Platt FM. 2010. Macroautophagy is not directly involved in the metabolism of amyloid precursor protein. J Biol Chem 285(48):37415-26. [PubMed: 20864542]  [MGI Ref ID J:167334]

Buccoliero R; Bodennec J; Van Echten-Deckert G; Sandhoff K; Futerman AH. 2004. Phospholipid synthesis is decreased in neuronal tissue in a mouse model of Sandhoff disease. J Neurochem 90(1):80-8. [PubMed: 15198669]  [MGI Ref ID J:91444]

Cachon-Gonzalez MB; Wang SZ; Ziegler R; Cheng SH; Cox TM. 2014. Reversibility of neuropathology in Tay-Sachs-related diseases. Hum Mol Genet 23(3):730-48. [PubMed: 24057669]  [MGI Ref ID J:204890]

De Santo C; Salio M; Masri SH; Lee LY; Dong T; Speak AO; Porubsky S; Booth S; Veerapen N; Besra GS; Grone HJ; Platt FM; Zambon M; Cerundolo V. 2008. Invariant NKT cells reduce the immunosuppressive activity of influenza A virus-induced myeloid-derived suppressor cells in mice and humans. J Clin Invest 118(12):4036-48. [PubMed: 19033672]  [MGI Ref ID J:144728]

Denny CA; Alroy J; Pawlyk BS; Sandberg MA; d'Azzo A; Seyfried TN. 2007. Neurochemical, morphological, and neurophysiological abnormalities in retinas of Sandhoff and GM1 gangliosidosis mice. J Neurochem 101(5):1294-302. [PubMed: 17442056]  [MGI Ref ID J:122595]

Denny CA; Heinecke KA; Kim YP; Baek RC; Loh KS; Butters TD; Bronson RT; Platt FM; Seyfried TN. 2010. Restricted ketogenic diet enhances the therapeutic action of N-butyldeoxynojirimycin towards brain GM2 accumulation in adult Sandhoff disease mice. J Neurochem 113(6):1525-35. [PubMed: 20374428]  [MGI Ref ID J:163568]

Gadola SD; Silk JD; Jeans A; Illarionov PA; Salio M; Besra GS; Dwek R; Butters TD; Platt FM; Cerundolo V. 2006. Impaired selection of invariant natural killer T cells in diverse mouse models of glycosphingolipid lysosomal storage diseases. J Exp Med 203(10):2293-303. [PubMed: 16982810]  [MGI Ref ID J:124639]

Gulinello M; Chen F; Dobrenis K. 2008. Early deficits in motor coordination and cognitive dysfunction in a mouse model of the neurodegenerative lysosomal storage disorder, Sandhoff disease. Behav Brain Res 193(2):315-9. [PubMed: 18611415]  [MGI Ref ID J:139219]

Hepbildikler ST; Sandhoff R; Kolzer M; Proia RL; Sandhoff K. 2002. Physiological substrates for human lysosomal beta -hexosaminidase S. J Biol Chem 277(4):2562-72. [PubMed: 11707436]  [MGI Ref ID J:124832]

Jeyakumar M; Butters TD; Cortina-Borja M; Hunnam V; Proia RL; Perry VH; Dwek RA; Platt FM. 1999. Delayed symptom onset and increased life expectancy in Sandhoff disease mice treated with N-butyldeoxynojirimycin. Proc Natl Acad Sci U S A 96(11):6388-93. [PubMed: 10339597]  [MGI Ref ID J:55198]

Keilani S; Lun Y; Stevens AC; Williams HN; Sjoberg ER; Khanna R; Valenzano KJ; Checler F; Buxbaum JD; Yanagisawa K; Lockhart DJ; Wustman BA; Gandy S. 2012. Lysosomal dysfunction in a mouse model of Sandhoff disease leads to accumulation of ganglioside-bound amyloid-beta peptide. J Neurosci 32(15):5223-36. [PubMed: 22496568]  [MGI Ref ID J:184450]

Liu Y; Wada R; Kawai H; Sango K; Deng C; Tai T; McDonald MP; Araujo K; Crawley JN; Bierfreund U; Sandhoff K; Suzuki K; Proia RL. 1999. A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder [see comments] J Clin Invest 103(4):497-505. [PubMed: 10021458]  [MGI Ref ID J:53080]

Mallevaey T; Zanetta JP; Faveeuw C; Fontaine J; Maes E; Platt F; Capron M; de-Moraes ML; Trottein F. 2006. Activation of invariant NKT cells by the helminth parasite schistosoma mansoni. J Immunol 176(4):2476-85. [PubMed: 16456008]  [MGI Ref ID J:129188]

Martino S; di Girolamo I; Cavazzin C; Tiribuzi R; Galli R; Rivaroli A; Valsecchi M; Sandhoff K; Sonnino S; Vescovi A; Gritti A; Orlacchio A. 2009. Neural precursor cell cultures from GM2 gangliosidosis animal models recapitulate the biochemical and molecular hallmarks of the brain pathology. J Neurochem 109(1):135-47. [PubMed: 19166507]  [MGI Ref ID J:149170]

Norflus F; Tifft CJ; McDonald MP; Goldstein G; Crawley JN; Hoffmann A; Sandhoff K; Suzuki K; Proia RL. 1998. Bone marrow transplantation prolongs life span and ameliorates neurologic manifestations in Sandhoff disease mice. J Clin Invest 101(9):1881-8. [PubMed: 9576752]  [MGI Ref ID J:47677]

Ogawa Y; Tanaka M; Tanabe M; Suzuki T; Togawa T; Fukushige T; Kanekura T; Sakuraba H; Oishi K. 2013. Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease. PLoS One 8(1):e55856. [PubMed: 23383290]  [MGI Ref ID J:195997]

Pelled D; Lloyd-Evans E; Riebeling C; Jeyakumar M; Platt FM; Futerman AH. 2003. Inhibition of calcium uptake via the sarco/endoplasmic reticulum Ca2+-ATPase in a mouse model of Sandhoff disease and prevention by treatment with N-butyldeoxynojirimycin. J Biol Chem 278(32):29496-501. [PubMed: 12756243]  [MGI Ref ID J:84955]

Pelled D; Riebeling C; van Echten-Deckert G; Sandhoff K; Futerman AH. 2003. Reduced rates of axonal and dendritic growth in embryonic hippocampal neurones cultured from a mouse model of Sandhoff disease. Neuropathol Appl Neurobiol 29(4):341-9. [PubMed: 12887594]  [MGI Ref ID J:103900]

Plati T; Visigalli I; Capotondo A; Buono M; Naldini L; Cosma MP; Biffi A. 2009. Development and maturation of invariant NKT cells in the presence of lysosomal engulfment. Eur J Immunol 39(10):2748-54. [PubMed: 19637231]  [MGI Ref ID J:153255]

Sango K; McDonald MP; Crawley JN; Mack ML; Tifft CJ; Skop E; Starr CM; Hoffmann A; Sandhoff K; Suzuki K; Proia RL. 1996. Mice lacking both subunits of lysosomal beta-hexosaminidase display gangliosidosis and mucopolysaccharidosis. Nat Genet 14(3):348-52. [PubMed: 8896570]  [MGI Ref ID J:36305]

Sango K; Takano M; Ajiki K; Tokashiki A; Arai N; Kawano H; Horie H; Yamanaka S. 2005. Impaired neurite outgrowth in the retina of a murine model of Sandhoff disease. Invest Ophthalmol Vis Sci 46(9):3420-5. [PubMed: 16123447]  [MGI Ref ID J:101337]

Sango K; Yamanaka S; Ajiki K; Tokashiki A; Watabe K. 2002. Lysosomal storage results in impaired survival but normal neurite outgrowth in dorsal root ganglion neurones from a mouse model of Sandhoff disease. Neuropathol Appl Neurobiol 28(1):23-34. [PubMed: 11849560]  [MGI Ref ID J:103802]

Sango K; Yamanaka S; Hoffmann A; Okuda Y; Grinberg A; Westphal H; McDonald MP; Crawley JN; Sandhoff K; Suzuki K; Proia RL.. 1995. Mouse models of Tay-Sachs and Sandhoff diseases differ in neurologic phenotype and ganglioside metabolism. Nat Genet 11(2):170-6. [PubMed: 7550345]  [MGI Ref ID J:29268]

Sargeant TJ; Drage DJ; Wang S; Apostolakis AA; Cox TM; Cachon-Gonzalez MB. 2012. Characterization of inducible models of Tay-Sachs and related disease. PLoS Genet 8(9):e1002943. [PubMed: 23028353]  [MGI Ref ID J:190450]

Sargeant TJ; Wang S; Bradley J; Smith NJ; Raha AA; McNair R; Ziegler RJ; Cheng SH; Cox TM; Cachon-Gonzalez MB. 2011. Adeno-associated virus-mediated expression of {beta}-hexosaminidase prevents neuronal loss in the Sandhoff mouse brain. Hum Mol Genet 20(22):4371-80. [PubMed: 21852247]  [MGI Ref ID J:176890]

Suzuki K; Iseki E; Katsuse O; Yamaguchi A; Katsuyama K; Aoki I; Yamanaka S; Kosaka K. 2003. Neuronal accumulation of alpha- and beta-synucleins in the brain of a GM2 gangliosidosis mouse model. Neuroreport 14(4):551-4. [PubMed: 12657883]  [MGI Ref ID J:89803]

Suzuki K; Sango K; Proia RL; Langaman C. 1997. Mice deficient in all forms of lysosomal beta-hexosaminidase show mucopolysaccharidosis-like pathology. J Neuropathol Exp Neurol 56(6):693-703. [PubMed: 9184660]  [MGI Ref ID J:41920]

Tsuji D; Kuroki A; Ishibashi Y; Itakura T; Kuwahara J; Yamanaka S; Itoh K. 2005. Specific induction of macrophage inflammatory protein 1-alpha in glial cells of Sandhoff disease model mice associated with accumulation of N-acetylhexosaminyl glycoconjugates. J Neurochem 92(6):1497-507. [PubMed: 15748167]  [MGI Ref ID J:96874]

Vitner EB; Dekel H; Zigdon H; Shachar T; Farfel-Becker T; Eilam R; Karlsson S; Futerman AH. 2010. Altered expression and distribution of cathepsins in neuronopathic forms of Gaucher disease and in other sphingolipidoses. Hum Mol Genet 19(18):3583-90. [PubMed: 20616152]  [MGI Ref ID J:163168]

Vitner EB; Salomon R; Farfel-Becker T; Meshcheriakova A; Ali M; Klein AD; Platt FM; Cox TM; Futerman AH. 2014. RIPK3 as a potential therapeutic target for Gaucher's disease. Nat Med 20(2):204-8. [PubMed: 24441827]  [MGI Ref ID J:206864]

Wada R; Tifft CJ; Proia RL. 2000. Microglial activation precedes acute neurodegeneration in sandhoff disease and is suppressed by bone marrow transplantation Proc Natl Acad Sci U S A 97(20):10954-9. [PubMed: 11005868]  [MGI Ref ID J:64739]

Wu YP; Mizugishi K; Bektas M; Sandhoff R; Proia RL. 2008. Sphingosine kinase 1/S1P receptor signaling axis controls glial proliferation in mice with Sandhoff disease. Hum Mol Genet 17(15):2257-64. [PubMed: 18424450]  [MGI Ref ID J:137634]

Wu YP; Proia RL. 2004. Deletion of macrophage-inflammatory protein 1 alpha retards neurodegeneration in Sandhoff disease mice. Proc Natl Acad Sci U S A 101(22):8425-30. [PubMed: 15155903]  [MGI Ref ID J:90687]

Yamaguchi A; Katsuyama K; Nagahama K; Takai T; Aoki I; Yamanaka S. 2004. Possible role of autoantibodies in the pathophysiology of GM2 gangliosidoses. J Clin Invest 113(2):200-8. [PubMed: 14722612]  [MGI Ref ID J:87617]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)