Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129S2-Seletm1Hyn/J    (Changed: 29-APR-09 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F15N1p Generation Definitions   Donating Investigator Richard Hynes,   Massachusetts Institute of Technology

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Sele^tm2Hyn targeted mutation are viable and fertile. Homozygous mutant mice show only subtle defects in leukocyte recruitment, unless P-selectin (Selp) is also ablated or blocked with antibody.

Development
The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells and maintained on a mixed C57BL/6 and 129S2 background.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Sele^tm2Hyn allele

008434	B6.129S2-Seletm2Hyn/J
003806	B6;129S-Seletm2Hyn Selltm4Hyn/J
008435	C.129S2(B6)-Seletm2Hyn/J

View Strains carrying   Sele^tm2Hyn     (3 strains)

Strains carrying other alleles of Sele

008238	129S-Seletm1Dmil/J
008437	B6.129S2-Seletm1Hyn Selptm1Hyn/J
008236	B6.129S4-Seletm1Dmil/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J
008237	C.129S4-Seletm1Dmil/J

View Strains carrying other alleles of Sele     (7 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Sele^tm2Hyn/Sele^tm2Hyn

        involves: 129S2/SvPas * C57BL/6

• immune system phenotype
• abnormal leukocyte rolling (MGI Ref ID J:57973)
  • there is an increase in the number of rolling leukocytes within the mesenteric venules of mice treated with TNF
• impaired neutrophil recruitment (MGI Ref ID J:57973)
  • neutrophil influx in thioglycollate-induced peritonitis is inhibited at 2 hours after injection

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Sele^tm2Hyn/Sele^tm2Hyn

        involves: 129S2/SvPas

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:31626)

Sele^tm2Hyn/Sele^tm2Hyn

        B6.129S2-Sele^tm2Hyn

• hematopoietic system phenotype
• decreased mast cell number (MGI Ref ID J:121083)
  • during the chronic phase of antigen exposure are decreased compared to in wild-type mice
• increased mast cell number (MGI Ref ID J:121083)
  • during the early phase of antigen exposure mast cell numbers are increased compared to in wild-type mice
  • however, treatment with P-selectin antibodies reduces the number of mast cells at the sight of inflammation
• immune system phenotype
• decreased mast cell number (MGI Ref ID J:121083)
  • during the chronic phase of antigen exposure are decreased compared to in wild-type mice
• increased mast cell number (MGI Ref ID J:121083)
  • during the early phase of antigen exposure mast cell numbers are increased compared to in wild-type mice
  • however, treatment with P-selectin antibodies reduces the number of mast cells at the sight of inflammation
• type IV hypersensitivity reaction (MGI Ref ID J:121083)
  • oxazolone-treated ears exhibit a more rapid onset of ear swelling and increased ear thickness between days 2 and 6 compared to similarly treated wild-type mice
  • acute inflammatory response to oxazolone exposure is exacerbated compared to in similarly treated wild-type mice
  • chronic inflammation in response to oxazolone exposure is inhibited compared to in similarly treated wild-type mice
  • 24 and 48 hours after elicitation, antigen-exposed ears of mice treated with P-selectin antibodies at 0-hour exhibit a 70% to 80% inhibition in swelling compared to in wild-type mice
  • 48 hours after elicitation, antigen-exposed ears of mice treated with P-selectin antibodies at 24-hour exhibit a modest inhibition in swelling compared to in wild-type mice
  • however, oxazolone-treated ears have the same thickness as similarly treated wild-type ears at day 8 or 10 of treatment

Genes & Alleles

Gene & Allele Information

Allele Symbol		Seletm2Hyn
Allele Name		targeted mutation 2, Richard O Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		E-selectin-;
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Sele, selectin, endothelial cell
Chromosome		1
Gene Common Name(s)		CD62E; E-selectin; ELAM; ELAM1; ESEL; Elam; LECAM2; endothelial adhesion molecule;
Molecular Note		A modified D3 ES cell line heterozygous for the Selptm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal mRNA for E-selectin but the presence of mRNA for P-selectin in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in trans with the Selptm1Hyn mutation and were subsequently bred apart. [MGI Ref ID J:31626]

Genotyping

Genotyping Information

Genotyping Protocols

Sele^tm1Hyn, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043]  [MGI Ref ID J:31626]

Additional References

Sele^tm2Hyn related

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58. [PubMed: 17456770]  [MGI Ref ID J:121083]

Hidalgo A; Peired AJ; Wild MK; Vestweber D; Frenette PS. 2007. Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4):477-89. [PubMed: 17442598]  [MGI Ref ID J:123577]

Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773]  [MGI Ref ID J:50212]

Horikawa M; Fujimoto M; Hasegawa M; Matsushita T; Hamaguchi Y; Kawasuji A; Matsushita Y; Fujita T; Ogawa F; Takehara K; Steeber DA; Sato S. 2006. E- and P-selectins synergistically inhibit bleomycin-induced pulmonary fibrosis. Am J Pathol 169(3):740-9. [PubMed: 16936251]  [MGI Ref ID J:112363]

Kaifi JT; Hall LR; Diaz C; Sypek J; Diaconu E; Lass JH; Pearlman E. 2000. Impaired eosinophil recruitment to the cornea in P-selectin-deficient mice in onchocerca volvulus keratitis (River blindness) Invest Ophthalmol Vis Sci 41(12):3856-61. [PubMed: 11053286]  [MGI Ref ID J:65551]

Komura K; Hasegawa M; Hamaguchi Y; Saito E; Kaburagi Y; Yanaba K; Kawara S; Takehara K; Seki M; Steeber DA; Tedder TF; Sato S. 2003. Ultraviolet light exposure suppresses contact hypersensitivity by abrogating endothelial intercellular adhesion molecule-1 up-regulation at the elicitation site. J Immunol 171(6):2855-62. [PubMed: 12960307]  [MGI Ref ID J:85377]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Tomita H; Iwata Y; Ogawa F; Komura K; Shimizu K; Yoshizaki A; Hara T; Muroi E; Yanaba K; Bae S; Takenaka M; Hasegawa M; Fujimoto M; Sato S. 2009. P-selectin glycoprotein ligand-1 contributes to wound healing predominantly as a p-selectin ligand and partly as an e-selectin ligand. J Invest Dermatol 129(8):2059-67. [PubMed: 19177138]  [MGI Ref ID J:152512]

Yanaba K; Kaburagi Y; Takehara K; Steeber DA; Tedder TF; Sato S. 2003. Relative contributions of selectins and intercellular adhesion molecule-1 to tissue injury induced by immune complex deposition. Am J Pathol 162(5):1463-73. [PubMed: 12707029]  [MGI Ref ID J:113592]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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