Description

Strain Information

Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Sibling x Sibling         (Female x Male)   01-MAR-06 Species laboratory mouse Background Strain C57BL/6J Donor Strain DBA/2N Generation B6N N13B6JN28F32+21 (19-NOV-09)   Donating Investigator Dr. Alan Poland,

Appearance
black
Related Genotype: a/a

Development
The Ahr^d allele was backcrossed from DBA/2N onto C57BL/6N via a backcross-intercross breeding scheme by Dr. Daniel Nebert. At generation N13 this congenic was then transferred to Dr Alan Poland et al., who then backcrossed the Ahr^d allele onto C57BL/6J, again via a backcross-intercross breeding scheme, and homozygotes at or beyond generation N17 were sent to The Jackson Laboratory in 1997 and maintained by sibling intercross.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Ahr^d allele

000648	AKR/J
000671	DBA/2J

View Strains carrying   Ahr^d     (2 strains)

Strains carrying other alleles of Ahr

000645	A/HeJ
000646	A/J
002920	B6(D2N).Spretus-Ahrb-3/J
006203	B6.129(FVB)-Ahrtm3.1Bra/J
002831	B6.129-Ahrtm1Bra/J
000130	B6.C-H17c/(HW14)ByJ
000136	B6.C-H34c/(HW22)ByJ
000370	B6.C-H38c/(HW119)ByJ
002727	B6;129-Ahrtm1Bra/J
001026	BALB/cByJ
000659	C3H/HeJ
000664	C57BL/6J
000669	C58/J
000926	CAROLI/EiJ
000351	CXB1/ByJ
000352	CXB2/ByJ
000353	CXB3/ByJ
000354	CXB4/ByJ
000355	CXB5/ByJ
000356	CXB6/ByJ
000357	CXB7/ByJ
002937	D2.B6-Ahrb-1/J
000677	MA/MyJ
000550	MOLF/EiJ
001146	SPRET/EiJ

View Strains carrying other alleles of Ahr     (25 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Ahr^d/Ahr^d

        B6.D2-Ahr^d

• life span-post-weaning/aging
• decreased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:113285)
  • unlike C57BL/6-Ahr^b-1 neonates, mice are not susceptible to lethality induced by in utero exposure to cHBB
• homeostasis/metabolism phenotype
• decreased physiological sensitivity to xenobiotics (MGI Ref ID J:113285)
  • following exposure to cHBB in utero, neonates exhibit a lesser increase in volume density of hemopoietic islands in the liver compared to in C57BL/6-Ahr^b-1 neonates
• decreased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:113285)
  • unlike C57BL/6-Ahr^b-1 neonates, mice are not susceptible to lethality induced by in utero exposure to cHBB

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Ahr^d/Ahr^d

        DBA/2J

• life span-post-weaning/aging
• decreased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:26440)
  • ice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects
• homeostasis/metabolism phenotype
• decreased physiological sensitivity to xenobiotics (MGI Ref ID J:132380)
  • mice are more resistant to the teratogenic effects of TCDD than Ahr^b-1 homozygotes with only 30% of mice exhibiting altered palate development compared to 100% of Ahr^b-1 homozygotes
  • mice from mothers treated with TCDD exhibit a similar incidence of hydronephrosis as Ahr^b-1 homozygotes but at a reduced severity
  • mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects
• decreased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:26440)
  • ice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

Ahr^d/Ahr^d

        AKR

• life span-post-weaning/aging
• decreased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:26440)
  • mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects
• homeostasis/metabolism phenotype
• decreased physiological sensitivity to xenobiotics (MGI Ref ID J:26440)
  • mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects
• decreased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:26440)
  • mice are resistant to the pathological effects of DMBA including mortality, morbidity and severity of effects

Ahr^d/Ahr^d

        involves: 129S1/SvImJ * C57BL/6J

• life span-post-weaning/aging
• increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:113356)
  • mice are sensitive to in utero exposure to DBP and at three months of age develop difficulty breathing, anemia, hypoxia, large thoracic masses, enlarged spleens, livers and lymph nodes, and must be euthanized
  • however, maternal genotype of Ahr^d/Ahr^d improves survival of offspring exposed to DBP in utero while maternal dietary exposure to I3C increased survival of offspring exposed to DBP in utero irregardless of offspring or dam genotype
• homeostasis/metabolism phenotype
• increased physiological sensitivity to xenobiotics (MGI Ref ID J:113356)
  • mice are sensitive to in utero exposure to DBP and at three months of age develop difficulty breathing, anemia, hypoxia, large thoracic masses, enlarged spleens, livers and lymph nodes, and must be euthanized
  • however, maternal genotype of Ahr^d/Ahr^d improves survival of offspring exposed to DBP in utero while maternal dietary exposure to I3C increased survival of offspring exposed to DBP in utero irregardless of offspring or dam genotype
• increased incidence of chemically-induced tumors (MGI Ref ID J:113356)
  • offspring exposed to DBP in utero exhibit an increased susceptibility to developing lymphomas
  • offspring exposed to DBP in utero exhibit increased rates of lung cancer (though lower than in offspring of Ahr^d/Ahr^d dams) and liver tumors in male mice
• increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:113356)
  • mice are sensitive to in utero exposure to DBP and at three months of age develop difficulty breathing, anemia, hypoxia, large thoracic masses, enlarged spleens, livers and lymph nodes, and must be euthanized
  • however, maternal genotype of Ahr^d/Ahr^d improves survival of offspring exposed to DBP in utero while maternal dietary exposure to I3C increased survival of offspring exposed to DBP in utero irregardless of offspring or dam genotype
• tumorigenesis
• increased incidence of chemically-induced tumors (MGI Ref ID J:113356)
  • offspring exposed to DBP in utero exhibit an increased susceptibility to developing lymphomas
  • offspring exposed to DBP in utero exhibit increased rates of lung cancer (though lower than in offspring of Ahr^d/Ahr^d dams) and liver tumors in male mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Ahr^d related

Metabolism Research

Research Tools
Toxicology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ahrd
Allele Name		d variant
Allele Type		Not Applicable
Common Name(s)		Ahd; Ahk; Ahhn; ah; in;
Gene Symbol and Name		Ahr, aryl-hydrocarbon receptor
Chromosome		12
Gene Common Name(s)		Ah; Ahh; Ahre; In; aromatic hydrocarbon responsiveness; aryl hydrocarbon hydroxylase; bHLHe76; dioxin receptor; inflammatory reactivity;
General Note		Compared with Ahrd/Ahrd mice, Ahrb/Ahrb individuals have a high inflammatory response to cutaneous application of dimethylbenzanthracene; a high susceptibility to methylcholanthrene- and benzopyrene-induced subcutaneous sarcomas and methylcholanthrene-induced lung tumors; an increased resistance to zoxazolamine-induced paralysis, lindane toxicity, and benzo[a]pyrene-induced aplastic anemia and leukemia; a high susceptibility to acetaminophen-induced hepatic necrosis and cataract formation; and an increased susceptibility to polycyclic hydrocarbon-induced birth defects, stillbirths, resorptions, decreased body weight, ovarian primordial oocyte depletion, and spermatozoal aberrations (J:5822). The Ahrballele is associated with increases in numerous metabolites of chemical carcinogens binding to DNA nucleotides (J:12156). The effectiveness of several mutagens for Salmonella in vitro is enhanced by presence of a liver fraction from Ahrb/Ahrb> mice treated with polycyclic hydrocarbons, but not from similarly treated Ahrd/Ahr mice (J:5564). In contrast, oral doses of benzopyrene cause a high rate of leukemia in Ahrd/Ahrd but not in Ahrd/Ahrd mice, probably because the carcinogenic metabolites produced in responsive Ahrb/Ahrd mice are rapidly degraded in the intestine and excreted in the feces (J:6074). Strain of origin - this allele was found in DBA/2J, AKR/J, 129, SWR, RF, NZB strains
Molecular Note		This allele encodes a 104 kDa receptor that is stabilized by molybdate and has an affinity for ligand 10-100 fold lower than that of the receptor produced by the C57BL/6J allele. PCR sequencing of cDNA revealed ten nucleotide differences between the coding sequences of the DBA/2J and C57BL/6J receptors. Five of the ten differences would cause amino acid changes. One of these, an apparent T to C transition replaces the opal termination codon in the C57BL/6J allele with an arginine codon in the DBA/2J allele. This change would extend translation of the DBA/2J mRNA by 43 amino acids, accounting for the larger size of the peptide produced by this allele (104 kDa vs 95 kDa for the C57BL/6J allele). A second T to C transition changes a leucine codon in the C57BL/6J allele to a proline codon in the DBA/2J allele, and would likely change secondary structure of the peptide and thus ligand affinity. [MGI Ref ID J:15153] [MGI Ref ID J:17460] [MGI Ref ID J:22144]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Poland A; Glover E. 1990. Characterization and strain distribution pattern of the murine Ah receptor specified by the Ahd and Ahb-3 alleles. Mol Pharmacol 38(3):306-12. [PubMed: 2169579]  [MGI Ref ID J:34840]

Poland A; Palen D; Glover E. 1994. Analysis of the four alleles of the murine aryl hydrocarbon receptor. Mol Pharmacol 46(5):915-21. [PubMed: 7969080]  [MGI Ref ID J:22144]

Additional References

Ahr^d related

Benedict WF; Considine N; Nebert DW. 1973. Genetic differences in aryl hydrocarbon hydroxylase induction and benzo(a)pyrene-produced tumorigenesis in the mouse. Mol Pharmacol 9(2):266-77. [PubMed: 4123113]  [MGI Ref ID J:84312]

Boobis AR; Nebert DW. 1976. Genetic differences in the metabolism of carcinogens and in the binding of benzo (a) pyrene metabolites to DNA. Adv Enzyme Regul 15:339-62. [PubMed: 1030186]  [MGI Ref ID J:12156]

Castro DJ; Lohr CV; Fischer KA; Pereira CB; Williams DE. 2008. Lymphoma and lung cancer in offspring born to pregnant mice dosed with dibenzo[a,l]pyrene: the importance of in utero vs. lactational exposure. Toxicol Appl Pharmacol 233(3):454-8. [PubMed: 18848954]  [MGI Ref ID J:143604]

Chang C; Smith DR; Prasad VS; Sidman CL; Nebert DW; Puga A. 1993. Ten nucleotide differences, five of which cause amino acid changes, are associated with the Ah receptor locus polymorphism of C57BL/6 and DBA/2 mice. Pharmacogenetics 3(6):312-21. [PubMed: 8148872]  [MGI Ref ID J:17460]

Curran CP; Miller KA; Dalton TP; Vorhees CV; Miller ML; Shertzer HG; Nebert DW. 2006. Genetic differences in lethality of newborn mice treated in utero with coplanar versus non-coplanar hexabromobiphenyl. Toxicol Sci 89(2):454-64. [PubMed: 16291824]  [MGI Ref ID J:113285]

Felton JS; Nebert DW. 1975. Mutagenesis of certain activated carcinogens in vitro associated with genetically mediated increases in monooxygenase activity and cytochrome P 1-450. J Biol Chem 250(17):6769-78. [PubMed: 808546]  [MGI Ref ID J:5564]

Gielen JE; Goujon FM; Nebert DW. 1972. Genetic regulation of aryl hydrocarbon hydroxylase induction. II. Simple Mendelian expression in mouse tissues in vivo. J Biol Chem 247(4):1125-37. [PubMed: 4110756]  [MGI Ref ID J:84250]

Goujon FM; Nebert DW; Gielen JE. 1972. Genetic expression of aryl hydrocarbon hydroxylase induction. IV. Interaction of various compounds with different forms of cytochrome P-450 and the effect on benzo(a)pyrene metabolism in vitro. Mol Pharmacol 8(6):667-80. [PubMed: 4118365]  [MGI Ref ID J:84252]

Harper PA; Golas CL; Okey AB. 1991. Ah receptor in mice genetically nonresponsive for cytochrome P4501A1 induction: cytosolic Ah receptor, transformation to the nuclear binding state, and induction of aryl hydrocarbon hydroxylase by halogenated and nonhalogenated aromatic hydrocarbons in embryonic tissues and cells. Mol Pharmacol 40(5):818-26. [PubMed: 1658612]  [MGI Ref ID J:2134]

Kouri RE; Rude TH; Joglekar R; Dansette PM; Jerina DM; Atlas SA; Owens IS; Nebert DW. 1978. 2,3,7,8-tetrachlorodibenzo-p-dioxin as cocarcinogen causing 3-methylcholanthrene-initiated subcutaneous tumors in mice genetically 'nonresponsive' at Ah locus. Cancer Res 38(9):2777-83. [PubMed: 679184]  [MGI Ref ID J:84318]

Moriguchi T; Motohashi H; Hosoya T; Nakajima O; Takahashi S; Ohsako S; Aoki Y; Nishimura N; Tohyama C; Fujii-Kuriyama Y; Yamamoto M. 2003. Distinct response to dioxin in an arylhydrocarbon receptor (AHR)-humanized mouse. Proc Natl Acad Sci U S A 100(10):5652-7. [PubMed: 12730383]  [MGI Ref ID J:132380]

Nebert DW; Atlas SA; Guenthner TM; Kouri RE. 1978. The Ah locus: genetic regulation of the enzymes which metabolize polycyclic hydrocarbons and the risk of cancer. In: Polycyclic Hydrocarbons and Cancer: Chemistry, Molecular Biology and Environment. Academic Press, New York.  [MGI Ref ID J:30693]

Nebert DW; Considine N; Owens IS. 1973. Genetic expression of aryl hydrocarbon hydroxylase induction. VI. Control of other aromatic hydrocarbon-inducible mono-oxygenase activities at or near the same genetic locus. Arch Biochem Biophys 157(1):148-59. [PubMed: 4716952]  [MGI Ref ID J:84313]

Nebert DW; Gelboin HV. 1969. The in vivo and in vitro induction of aryl hydrocarbon hydroxylase in mammalian cells of different species, tissues, strains, and developmental and hormonal states. Arch Biochem Biophys 134(1):76-89. [PubMed: 4981257]  [MGI Ref ID J:84248]

Nebert DW; Gielen JE. 1972. Genetic regulation of aryl hydrocarbon hydroxylase induction in the mouse. Fed Proc 31(4):1315-25. [PubMed: 4114109]  [MGI Ref ID J:5282]

Nebert DW; Gielen JE; Goujon FM. 1972. Genetic expression of aryl hydrocarbon hydroxylase induction. 3. Changes in the binding of n-octylamine to cytochrome P-450. Mol Pharmacol 8(6):651-66. [PubMed: 4118364]  [MGI Ref ID J:84251]

Nebert DW; Goujon FM; Gielen JE. 1972. Aryl hydrocarbon hydroxylase induction by polycyclic hydrocarbons: simple autosomal dominant trait in the mouse. Nat New Biol 236(65):107-10. [PubMed: 4502804]  [MGI Ref ID J:84249]

Nebert DW; Jensen NM. 1979. Benzo[a]pyrene-initiated leukemia in mice. Association with allelic differences at the Ah locus. Biochem Pharmacol 28(1):149-51. [PubMed: 758905]  [MGI Ref ID J:6074]

Nebert DW; Jensen NM; Shinozuka H; Kunz HW; Gill TJ 3d. 1982. The Ah phenotype. Survey of forty-eight rat strains and twenty inbred mouse strains. Genetics 100(1):79-87. [PubMed: 7095422]  [MGI Ref ID J:6809]

Nebert DW; Kon H. 1973. Genetic regulation of aryl hydrocarbon hydroxylase induction. V. Specific changes in spin state of cytochrome P 450 from genetically responsive animals. J Biol Chem 248(1):169-78. [PubMed: 4348203]  [MGI Ref ID J:84311]

Nebert DW; Robinson JR; Niwa A; Kumaki K; Poland AP. 1975. Genetic expression of aryl hydrocarbon hydroxylase activity in the mouse. J Cell Physiol 85(2 Pt 2 Suppl 1):393-414. [PubMed: 1091656]  [MGI Ref ID J:84317]

Niwa A; Kumaki K; Nebert DW; Poland AP. 1975. Genetic expression of aryl hydrocarbon hydroxylase activity in the mouse. Distinction between the 'responsive' homozygote and heterozygote at the Ah locus. Arch Biochem Biophys 166(2):559-64. [PubMed: 1119809]  [MGI Ref ID J:84316]

Oesch F; Morris N; Daly JW. 1973. Genetic expression of the induction of epoxide hydrase and aryl hydrocarbon hydroxylase activities in the mouse by phenobarbital or 3-methylcholanthrene. Mol Pharmacol 9(5):629-6. [PubMed: 4788156]  [MGI Ref ID J:25852]

Okey AB; Vella LM; Harper PA. 1989. Detection and characterization of a low affinity form of cytosolic Ah receptor in livers of mice nonresponsive to induction of cytochrome P1-450 by 3-methylcholanthrene. Mol Pharmacol 35(6):823-30. [PubMed: 2543914]  [MGI Ref ID J:27899]

Poel WE; Stanton D; Peters E; Wade HO. 1958. Comparative susceptibilities of seven inbred strains of mice to qualified applications of 3, 4-benzpyrene Proc Am Assoc Cancer Res 2:335.  [MGI Ref ID J:84245]

Poland A; Bradfield C. 1992. A brief review of the Ah locus. Tohoku J Exp Med 168(2):83-7. [PubMed: 1339107]  [MGI Ref ID J:12546]

Poland A; Glover E; Kende AS. 1976. Stereospecific, high affinity binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin by hepatic cytosol. Evidence that the binding species is receptor for induction of aryl hydrocarbon hydroxylase. J Biol Chem 251(16):4936-46. [PubMed: 956169]  [MGI Ref ID J:84247]

Poland A; Glover E; Taylor BA. 1987. The murine Ah locus: a new allele and mapping to chromosome 12. Mol Pharmacol 32(4):471-8. [PubMed: 2823093]  [MGI Ref ID J:8895]

Poland A; Teitelbaum P; Glover E; Kende A. 1989. Stimulation of in vivo hepatic uptake and in vitro hepatic binding of [125I]2-lodo-3,7,8-trichlorodibenzo-p-dioxin by the administration of agonist for the Ah receptor. Mol Pharmacol 36(1):121-7. [PubMed: 2546046]  [MGI Ref ID J:126377]

Poland AP; Glover E; Robinson JR; Nebert DW. 1974. Genetic expression of aryl hydrocarbon hydroxylase activity. Induction of monooxygenase activities and cytochrome P1-450 formation by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice genetically 'nonresponsive' to other aromatic hydrocarbons. J Biol Chem 249(17):5599-606. [PubMed: 4370044]  [MGI Ref ID J:84314]

Quintana FJ; Basso AS; Iglesias AH; Korn T; Farez MF; Bettelli E; Caccamo M; Oukka M; Weiner HL. 2008. Control of T(reg) and T(H)17 cell differentiation by the aryl hydrocarbon receptor. Nature 453(7191):65-71. [PubMed: 18362915]  [MGI Ref ID J:136052]

Robinson JR; Considine N; Nebert DW. 1974. Genetic expression of aryl hydrocarbon hydroxylase induction. Evidence for the involvement of other genetic loci. J Biol Chem 249(18):5851-9. [PubMed: 4413562]  [MGI Ref ID J:84315]

Schmid FA; Demetriades MS; Schabel FM rd; Tarnowski GS. 1967. Toxicity of several cancerigenic polycyclic hydrocarbons and other agents in AKR and C57BL-6 mice. Cancer Res 27(3):563-7. [PubMed: 6021514]  [MGI Ref ID J:84246]

Schmid FA; Elmer I; Tarnowski GS. 1969. Genetic determination of differential inflammatory reactivity and subcutaneous tumor susceptibility of AKR-J and C57BL-6J mice to 7,12-dimethylbenz- [a]anthracene. Cancer Res 29(8):1585-9. [PubMed: 5807232]  [MGI Ref ID J:34134]

Schmid FA; Pena RC; Robinson W; Tarnowski GS. 1967. Toxicity of intraperitoneal injections of 7, 12-dimethylbenz[a]anthracene in inbred mice. Cancer Res 27(3):558-62. [PubMed: 6021513]  [MGI Ref ID J:26440]

Schmidt JV; Carver LA; Bradfield CA. 1993. Molecular characterization of the murine Ahr gene. Organization, promoter analysis, and chromosomal assignment. J Biol Chem 268(29):22203-9. [PubMed: 8408082]  [MGI Ref ID J:15153]

Shi Z; Chen Y; Dong H; Amos-Kroohs RM; Nebert DW. 2008. Generation of a 'humanized' hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mouse line harboring the poor-affinity aryl hydrocarbon receptor. Biochem Biophys Res Commun 376(4):775-80. [PubMed: 18814841]  [MGI Ref ID J:141523]

Taylor BA. 1971. Strain distribution and linkage tests of 7,12-dimethylbenzanthracene (DMBA) inflammatory response in mice. Life Sci I 10(19):1127-34. [PubMed: 5132702]  [MGI Ref ID J:5244]

Thomas PE; Hutton JJ; Taylor BA. 1973. Genetic relationship between aryl hydrocarbon hydroxylase inducibility and chemical carcinogen induced skin ulceration in mice. Genetics 74(4):655-9. [PubMed: 4750810]  [MGI Ref ID J:5387]

Thomas PE; Kouri RE; Hutton JJ. 1972. The genetics of aryl hydrocarbon hydroxylase induction in mice: a single gene difference between C57BL-6J and DBA-2J. Biochem Genet 6(2):157-68. [PubMed: 4666754]  [MGI Ref ID J:31977]

Thorgeirsson SS; Nebert DW. 1977. The Ah locus and the metabolism of chemical carcinogens and other foreign compounds. Adv Cancer Res 25:149-93. [PubMed: 405846]  [MGI Ref ID J:5822]

Walisser JA; Bunger MK; Glover E; Bradfield CA. 2004. Gestational exposure of Ahr and Arnt hypomorphs to dioxin rescues vascular development. Proc Natl Acad Sci U S A 101(47):16677-82. [PubMed: 15545609]  [MGI Ref ID J:94465]

Yeager RL; Reisman SA; Aleksunes LM; Klaassen CD. 2009. Introducing the 'TCDD-inducible AhR-Nrf2 gene battery'. Toxicol Sci 111(2):238-46. [PubMed: 19474220]  [MGI Ref ID J:154083]

Yu Z; Mahadevan B; Lohr CV; Fischer KA; Louderback MA; Krueger SK; Pereira CB; Albershardt DJ; Baird WM; Bailey GS; Williams DE. 2006. Indole-3-carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon dibenzo[a,l]pyrene. Carcinogenesis 27(10):2116-23. [PubMed: 16704990]  [MGI Ref ID J:113356]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Mating System	Sibling x Sibling         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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