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Strain Name:

B6.129P2-Cd40tm1Kik/J

Stock Number:

002928

Availability:

Repository- Live

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General Terms and Conditions

Former Name      B6.129P2-Tnfrsf5tm1Kik/J    (Changed: 30-SEP-05 )
      Cd40    (Changed: 15-DEC-04 )
Genes & Alleles   Cd40;   Cd40tm1Kik;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain C57BL/6Ncr
Donor Strain 129P2 via E14-1 ES cell line
Donating Investigator Hitoshi Kikutani,   Osaka University
GenerationN12F24 (20-DEC-06)

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice exhibit impaired immunoglobulin class switching and germinal center formation.

Mammalian Phenotype Terms assigned by genotype

Cd40tm1Kik/Cd40tm1Kik

        B6.129P2-Cd40tm1Kik/J
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype (MGI Ref ID J:75152)
    • normal thrombogenesis in ferric-chloride-induced injury of mesenteric arterioles
  • immune system phenotype
  • abnormal dendritic cell physiology (MGI Ref ID J:86998)
    • impaired production of IL-12 after Mycobacterium tuberculosis infection and after incubation with Hsp7
    • impaired priming of IFN-gamma producing T cells
  • increased susceptibility to bacterial infection (MGI Ref ID J:86998)
    • increased susceptibility to aerosol Mycobacterium tuberculosis infection
    • increased bacterial loads in lung and spleen
    • 40% of infected mice moribund 3 - 4 weeks postinfection

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Cd40tm1Kik/Cd40tm1Kik

        involves: 129P2/OlaHsd * C57BL/6
  • hematopoietic system phenotype
  • absent spleen germinal center (MGI Ref ID J:25007)
    • failed to form
  • decreased neutrophil cell number (MGI Ref ID J:25007)
    • in non-SPF facilities compared to wild-type controls
  • immune system phenotype
  • *normal* immune system phenotype (MGI Ref ID J:25007)
    • normal numbers and ratios of T and B cells
    • abnormal humoral immune response (MGI Ref ID J:25007)
      • decreased immunoglobulin level (MGI Ref ID J:25007)
        • reduced production of IgG1, IgG2a, IgG2b, IgG3, IgA, IgE in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
        • response to thymus-independent (TI) antigens was in tact
        • decreased IgA level (MGI Ref ID J:25007)
          • reduced production in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
          • decreased serum concentrations in unimmunized mice
        • decreased IgE level (MGI Ref ID J:25007)
          • reduced production in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
          • decreased serum concentrations in unimmunized mice
        • decreased IgG1 level (MGI Ref ID J:25007)
          • reduced production of IgG1 in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
          • decreased serum concentrations of IgG1 in unimmunized mice
        • decreased IgG2a level (MGI Ref ID J:25007)
          • reduced production of IgG2a in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
          • decreased serum concentrations of IgG2a in unimmunized mice
        • decreased IgG2b level (MGI Ref ID J:25007)
          • reduced production of IgG2b in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
          • decreased serum concentrations of IgG2b in unimmunized mice
        • decreased IgG3 level (MGI Ref ID J:25007)
          • reduced production of IgG3 in response to thymus-dependent (TD) antigen, dintrophenol-conjugated ovalbumin
          • IgG3 serum concentrations in unimmunized mice are not decreased
    • absent spleen germinal center (MGI Ref ID J:25007)
      • failed to form
    • decreased neutrophil cell number (MGI Ref ID J:25007)
      • in non-SPF facilities compared to wild-type controls
    • decreased susceptibility to type II hypersensitivity reaction (MGI Ref ID J:61673)
      • in model of accelerated anti-glomerular basement membrane antibody-mediated glomerulonephritis
      • deposition of IgG and complement in glomeruli not observed
      • glomeruli almost normal
      • fibrin deposition in glomeruli not observed
      • failure to induce proteinuria

Gene & Allele Details

Allele Symbol Cd40tm1Kik
Allele Name targeted mutation 1, Hitoshi Kikutani
Common Name(s) CD40 Ko; CD40-; CD40KO; Tnfrsf5-;
Mutation Made By Hitoshi Kikutani,   Osaka University
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Cd40, CD40 antigen
Chromosome 2
Gene Common Name(s) AI326936; Bp50; CDW40; MGC9013; TNFRSF5; Tnfrsf5; expressed sequence AI326936; p50; tumor necrosis factor receptor superfamily, member 5;
Molecular Note A neomycin cassette was inserted into exon 3. Disruption of the functionality of the protein was confirmed by a surface expression assay in spleen cells in which no binding of Tnfsf5 to ligand was observed. [MGI Ref ID J:25007]

Control Information

  Allele   Control
 Cd40tm1Kik  000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Cd40tm1Kik

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Cd40tm1Kik allele
002927   CNCr.129P2-Cd40tm1Kik/J
View Strains carrying   Cd40tm1Kik     (1 strain)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cd40tm1Kik related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Growth Factors/Receptors/Cytokines

References

Selected Reference(s)

Kawabe T; Naka T; Yoshida K; Tanaka T; Fujiwara H; Suematsu S; Yoshida N; Kishimoto T; Kikutani H. 1994. The immune responses in CD40-deficient mice: impaired immunoglobulin class switching and germinal center formation. Immunity 1(3):167-78. [PubMed: 7534202]  [MGI Ref ID J:25007]

Additional References

Price and Supply Information

Strain Name: B6.129P2-Cd40tm1Kik/J
Stock Number: 002928

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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