Strain Name: |
FVB.129S2(B6)-Ccnd1tm1Wbg/J |
|---|---|
Stock Number: |
002935 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Ccnd1; Ccnd1tm1Wbg; Pde6b; Pde6brd1; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain FVB/NJ Donor Strain B6;129S-Ccnd1tm1Wbg (129S2 derived D3 ES cell line) Donating Investigator The Jackson Laboratory, Appearance
albino
Related Genotype: Tyrc/TyrcImportant Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.Strain Description
Mice homozygous for the targeted mutation develop to term but show growth retardation that is most pronounced by three weeks of age. The majority of mutant mice die early in life, often within the first month. Survivors continue to show lower than average weight and increased mortality. Mammary gland epithelial duct development is normal in virgin mutant mice. Mutant females that survive to adulthood are fertile, but mammary glands of pregnant mice show a dramatic impairment in the expansion of alveolar lobes and mice are unable to lactate. Steroid hormone levels are normal, and there is no apparent defect in estrogen receptor number, suggesting that cyclin D1 deficiency has an effect on the target tissue directly. Mutant mice demonstrate a neurological abnormality evidenced by limb retraction when lifted by their tails. The most severely affected animals remain in a clasped, flexed position for a few seconds after they have been returned to their cage. Retinal abnormalities include a dramatic reduction in cell numbers in all cell layers of the neural retina. This is due to the severely reduced ability of mutant retinal cell precursors to proliferate during embryonic development. The thickness of the inner plexiform layer is also reduced. The cells within the retina respond properly to light.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A 3700 bp EagI fragment of the mouse cyclin D1 gene, (5' to the first coding exon) was used in the targeting vector, and electoporated into D3 ES cells. Cyclin D1 heterozygous ES cells ere injected into C57BL/6 embryos, and the resulting chimeras were bred to B6 mice. The resulting cyclin D1 heterozygotes were bred to produce homozygous mice. The targeting vector deletes coding portions of exons I-III of the cyclin D1 gene and replaces them with the neomycin resistance gene. (Mam. Gland DB)
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Ccnd1tm1Wbg | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Robert Weinberg | ||
| Common Name(s) | CD1-; cyclin D1-; | ||
| Mutation Made By | Robert Weinberg, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Ccnd1, cyclin D1 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AI327039; BCL1; Cyl-1; D11S287E; PRAD1; U21B31; bcl-1; cD1; expressed sequence AI327039; | ||
| Molecular Note | A genomic fragment containing part of exon 1, exon 2 and exon 3 was replaced with a neomycin selection cassette. The deleted sequences encode the cyclin box domain thought to be required for protein activity. Western blot analysis on embryonic fibroblasts derived from homozygous mice confirmed that no stable protein was made from this allele. [J:28419] | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Ccndtm1Wbg
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, heterozygous mice can be bred with FVB/NJ. Expected coat color from breeding:Albino. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Ccnd1tm1Wbg allele
002537 B6;129S2-Ccnd1tm1Wbg/J View Strains carrying Ccnd1tm1Wbg (1 strain)
005143 C3.B6-Tg(Fabp1-Ccnd1)4Rdb/J 004834 C57BL/6-Tg(Fabp1-Ccnd1)4Rdb/J
004297 B6.CXB1-Pde6brd10/J 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:Ccnd1tm1Wbg related
Pde6brd1 relatedCancer Research
Genes Regulating Growth and Proliferation
Other
Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation
Developmental Biology Research
Eye Defects
Growth Defects
Postnatal Mortality
Sensorineural Research
Eye Defects
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
References
Selected Reference(s)
Additional ReferencesSicinski P; Donaher JL; Parker SB; Li T; Fazeli A; Gardner H; Haslam SZ; Bronson RT; Elledge SJ; Weinberg RA. 1995. Cyclin D1 provides a link between development and oncogenesis in the retina and breast. Cell 82(4):621-30. [PubMed: 7664341] [J:28419]
Price and Supply Information
| Strain Name: | FVB.129S2(B6)-Ccnd1tm1Wbg/J |
| Stock Number: | 002935 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form