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Strain Name:

B6.129-Kdrtm1Jrt/J

Stock Number:

002938

Availability:

Repository-Cryopreserved

Price and Supply Information

General Terms and Conditions

Genes & Alleles   Kdr;   Kdrtm1Jrt;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129X1 x 129S1 via R1 (+Kitl-SlJ) ES cell line
Donating Investigator IMR Colony,   The Jackson Laboratory
GenerationN?+12 (07-NOV-04)

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the Kdrtm1Jrt targeted mutation (formerly called Flk1) die at ~E8.5-E9.5 due to defects in hematopoietic and endothelial development. Embryos lack blood islands at E7.5 and fail to form organized blood vessels. There is severe reduction in hematopoietic progenitor cells.

Strain Development
This strain was developed in the lab of Dr. Janet Rossant at the University of Toronto. The insertion vector contains lacZ and neo.

Mammalian Phenotype Terms assigned by genotype

Kdrtm1Jrt/Kdrtm1Jrt

        B6.129-Kdrtm1Jrt/J
  • cardiovascular system phenotype
  • abnormal dorsal aorta morphology (MGI Ref ID J:96124)
    • at E8.5, dorsal aorta is absent
  • absent organized vascular network (MGI Ref ID J:96124)
    • at E8.5 vessels in the head as well as the dorsal aorta and vitelline artery are absent
    • at E9.5 no blood vessels are seen
  • embryogenesis phenotype
  • abnormal mesoderm development (MGI Ref ID J:96124)
    • at E7.5 the extraembryonic mesoderm consists of only a single cell layer
  • absent blood islands (MGI Ref ID J:96124)
    • at E7.5
  • absent vitelline blood vessels (MGI Ref ID J:96124)
    • at E9.5
  • hematopoietic system phenotype
  • impaired hematopoiesis (MGI Ref ID J:96124)
    • the number of hematopoietic progenitors and the number of erythoid, granulocyte/macrophage and mixed colonies formed are severely reduced

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Kdrtm1Jrt/Kdr+

        involves: 129S1/Sv * 129X1/SvJ
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:26846)

Kdrtm1Jrt/Kdrtm1Jrt

        involves: 129S1/Sv * 129X1/SvJ
  • lethality-prenatal/perinatal
  • embryonic lethality during organogenesis (MGI Ref ID J:26846)
    • no embryos are recovered at E10.5 but are present at E9.5
  • embryogenesis phenotype
  • abnormal allantois morphology (MGI Ref ID J:125783)
    • allantois explants fail to form any endothelial structures
  • abnormal embryonic tissue morphology (MGI Ref ID J:26846)
  • absent blood islands (MGI Ref ID J:26846)
  • absent vitelline blood vessels (MGI Ref ID J:26846)
    • at E8.5, yolk sac lack blood vessels and consist of a single layer of mesothelium lining the yolk sac endothelium
  • reduced embryo size (MGI Ref ID J:26846)
    • at E9.5
  • cardiovascular system phenotype
  • absent organized vascular network (MGI Ref ID J:26846)
  • congestive heart failure (MGI Ref ID J:26846)
    • embryos exhibit necrosis likely from cardiac failure
  • hematopoietic system phenotype
  • abnormal hematopoiesis (MGI Ref ID J:26846)
    • expression of markers for hematopiesis could not be detected at E8.5
    • at E8.5 only very rare committed hematopoietic cells could be identified by methylcellulose colony assay
  • growth/size phenotype
  • reduced embryo size (MGI Ref ID J:26846)
    • at E9.5
  • cellular phenotype
  • necrosis (MGI Ref ID J:26846)
    • embryos exhibit necrosis likely from cardiac failure

Kdrtm1Jrt/Kdrtm1Jrt

        Background Not Specified
  • cardiovascular system phenotype
  • absent organized vascular network (MGI Ref ID J:95387)
    • Kdr+ cells (lacZ expressing) reach the anterior portion portion of the embryo but no blood vessels develop

Gene & Allele Details

Allele Symbol Kdrtm1Jrt
Allele Name targeted mutation 1, Janet Rossant
Common Name(s) Flk1-; Flk1-lacZ neo-in; Flk1lacZ; Flk1lacZ; VEGFR-2-; flk-1-; flk.lacZ;
Mutation Made By Janet Rossant,   The Hospital for Sick Children
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Site of ExpressionThe vascular endothelial lineage.
Gene Symbol and Name Kdr, kinase insert domain protein receptor
Chromosome 5
Gene Common Name(s) CD309; FLK1; Flk-1; Flk1; MGC93590; VEGF receptor-2; VEGFR; VEGFR-2; VEGFR2; fetal liver kinase 1; vascular endothelial growth factor receptor- 2;
Molecular Note Insertion of an in-frame lacZ gene and a floxed PGK-neomycin resistance cassette replaced the translated portion of the first coding exon and the proximal part of the next intron, and placed lacZ under the transcriptional control of the endogenous promoter. Neither RT-PCR using total RNA nor Western blot analysis using a carboxy terminal antibody detected gene expression in E8.5 homozygous mutant embryos. [MGI Ref ID J:26846]

Control Information

  Allele   Control
 Kdrtm1Jrt  Wild-type from the colony
 Kdrtm1Jrt  000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Kdrtm1Jrt

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

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006431   B6.129P2-Adam21tm1Dgen/J
005770   B6.129P2-Adamts4tm1Dgen/J
005771   B6.129P2-Adamts5tm1Dgen/J
005773   B6.129P2-Adcy3tm1Dgen/J
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005775   B6.129P2-Adipor2tm1Dgen/J
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005777   B6.129P2-Axltm1Dgen/J
005783   B6.129P2-Cacna1ctm1Dgen/J
005780   B6.129P2-Cacna2d3tm1Dgen/J
005781   B6.129P2-Cacng3tm1Dgen/J
005782   B6.129P2-Cacng4tm1Dgen/J
005784   B6.129P2-Capn5tm1Dgen/J
005785   B6.129P2-Capn7tm1Dgen/J
005792   B6.129P2-Ccr1l1tm1Dgen/J
005793   B6.129P2-Ccr6tm1Dgen/J
005794   B6.129P2-Ccr7tm1Dgen/J
005779   B6.129P2-Celsr2tm1Dgen/J
005797   B6.129P2-Chrna2tm1Dgen/J
005787   B6.129P2-Ctsctm1Dgen/J
005796   B6.129P2-Cxcr3tm1Dgen/J
005798   B6.129P2-Drd5tm1Dgen/J
005800   B6.129P2-Efemp2tm1Dgen/J
005801   B6.129P2-Esrratm1Dgen/J
005802   B6.129P2-Faim2tm1Dgen/J
006262   B6.129P2-Fut2tm1Sdo/J
005803   B6.129P2-Fzd1tm1Dgen/J
005804   B6.129P2-Fzd8tm1Dgen/J
005811   B6.129P2-Gabra3tm1Dgen/J
005812   B6.129P2-Gabra4tm1Dgen/J
005810   B6.129P2-Gabrptm1Dgen/J
005809   B6.129P2-Galr1tm1Dgen/J
005816   B6.129P2-Glra3tm1Dgen/J
005805   B6.129P2-Gpr151tm1Dgen/J
005806   B6.129P2-Gpr37tm1Dgen/J
005807   B6.129P2-Gpr6tm1Dgen/J
005813   B6.129P2-Grik5tm1Dgen/J
005808   B6.129P2-Grk5tm1Dgen/J
005814   B6.129P2-Grm1tm1Dgen/J
005815   B6.129P2-Grm3tm1Dgen/J
005817   B6.129P2-Gsk3btm1Dgen/J
005818   B6.129P2-Hcrtr1tm1Dgen/J
005767   B6.129P2-Htr4tm1Dgen/J
005769   B6.129P2-Htr7tm1Dgen/J
005830   B6.129P2-Kcnq2tm1Dgen/J
005821   B6.129P2-Lats2tm1Dgen/J
005822   B6.129P2-Lmbr1tm1Dgen/J
005850   B6.129P2-Mapkapk2tm1Dgen/J
005824   B6.129P2-Mmp17tm1Dgen/J
005825   B6.129P2-Mtmr1tm1Dgen/J
005778   B6.129P2-Naip1tm1Dgen/J
005826   B6.129P2-Ntsr1tm1Dgen/J
005829   B6.129P2-Pkd2l2tm1Dgen/J
005828   B6.129P2-Ppardtm1Dgen/J
005831   B6.129P2-Ppm1ftm1Dgen/J
005827   B6.129P2-Ptch2tm1Dgen/J
005832   B6.129P2-Ptprotm1Dgen/J
005799   B6.129P2-S1pr4tm1Dgen/J
005837   B6.129P2-Scn11atm1Dgen/J
005836   B6.129P2-Scn9atm1Dgen/J
005834   B6.129P2-Sema5atm1Dgen/J
005835   B6.129P2-Sema6ctm1Dgen/J
006432   B6.129P2-Slc18a1tm1Dgen/J
005839   B6.129P2-Slc22a12tm1Dgen/J
005838   B6.129P2-Slc22a6tm1Dgen/J
005840   B6.129P2-Slc40a1tm1Dgen/J
005841   B6.129P2-Slc6a9tm1Dgen/J
005842   B6.129P2-Slc7a8tm1Dgen/J
005843   B6.129P2-Slc9a6tm1Dgen/J
005844   B6.129P2-Sstr1tm1Dgen/J
005847   B6.129P2-Tgfbr1tm1Dgen/J
005845   B6.129P2-Thbs4tm1Dgen/J
005790   B6.129P2-Tpp1tm1Dgen/J
005848   B6.129P2-Trpm5tm1Dgen/J
005791   B6.129P2-Xcr1tm1Dgen/J
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005901   B6.129S4-Ppardtm2Rev/J
006142   B6.129S4-Ppargtm1Rev/J
003754   B6.129S4-Shroom3Gt(ROSA)53Sor/J
005119   B6.129S6-Npas2tm1Slm/J
002741   B6.129S7-Alpltm1Sor/J
005970   B6.129S7-Atoh1tm2Hzo/J
006039   B6.129S7-Efnb2tm1And/J
002192   B6.129S7-Gt(ROSA)26Sor/J
005981   B6.129S7-Rai1tm1Jrl/J
005039   B6.129X1-Adra1atm1Pcs/J
005085   B6.Cg-Cd44tm1Hbg/J
007745   B6.Cg-Mirn155tm1.1Rsky/J
005317   B6.Cg-Tg(BAT-lacZ)3Picc/J
006055   B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006477   B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J
003139   B6.Cg-Tg(DBHn-lacZ)8Rpk/J
006229   B6.Cg-Tg(DRE-lacZ)2Gswz/J
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005788   B6;129P2-Cd97tm1Dgen/J
005833   B6;129P2-Rgs4tm1Dgen/J
002073   B6;129S-Gt(ROSA)26Sor/J
006470   B6;129S-Hopxtm1Eno/J
004153   B6;129S-Mtap7Gt(ROSABetageo)1Sor/J
006958   B6;129S-Nkd1tm1Kwha/J
006960   B6;129S-Nkd2tm1Kwha/J
007204   B6;129S4-2610005L07RikGt(ROSA)73Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004365   B6;129S6-Srebf1tm1Mbr/J
002317   B6;129S7-Alpltm1Sor/J
003266   B6;129S7-Epas1tm1Rus/J
006044   B6;129S7-Ephb4tm1And/J
003471   B6;C3H-Tg(CNP-GEO)1Ldh/J
006465   B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
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006671   B6;CBA-Tg(Olfr16*,taulacZ)5Mom/MomJ
006672   B6;CBA-Tg(Olfr16*,taulacZ)7Mom/MomJ
006673   B6;CBA-Tg(Olfr16,taulacZ)sn2Mom/MomJ
004141   B6;CBA-Tg(UAS-lacZ)65Rth/J
002369   B6;SJL-Tg(c177-lacZ)226Bri/J
002372   B6;SJL-Tg(c177-lacZ)227Bri/J
002621   B6;SJL-Tg(tetop-lacZ)2Mam/J
003299   B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J
002865   B6CBA-Tg(Wnt1-lacZ)206Amc/J
002955   C.129S7-Gt(ROSA)26Sor/J
002754   C57BL/6-Tg(LacZpl)60Vij/J
002193   C57BL/6J-Tg(MTn-lacZ)204Bri/J
002981   DBA/2-Tg(xstpx-lacZ)36And/J
004127   FVB-Tg(Nes-rtTA)306Rvs/J
007225   FVB.129(B6)-Usp18tm1Dzh/J
008203   FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)63Ahmb Tg(SMN2)89Ahmb/J
008209   FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)69Ahmb Tg(SMN2)89Ahmb/J
006214   FVB.Cg-Smn1tm1Msd/J
005024   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd/J
005026   FVB.Cg-Tg(SMN2)89Ahmb Tg(SMN1*A2G)2023Ahmb Smn1tm1Msd/J
005025   FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J
003140   FVB/N-Tg(PAI1-lacZ)1Jjb/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
005941   FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
003315   FVB/N-Tg(tetORo1-lacZ)3Conk/J
003487   FVB/NJ-Tg(XGFAP-lacZ)3Mes/J
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003899   STOCK Cd44tm1Hbg/J
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006578   STOCK Myoz2tm1Eno/J
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005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
006613   STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J
003919   STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
004623   STOCK Tg(Fos-lacZ)34Efu/J
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005493   STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
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View lacZ Expression Strains     (174 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects (hematopoietic defects)
Lymphoid Tissue Defects (hematopoietic defects)

Hematological Research
Hematopoietic Defects

Research Tools
lacZ Expression
Hematological Research

Kdrtm1Jrt related

Cancer Research
Growth Factors/Receptors/Cytokines

Cardiovascular Research
Vascular Defects

Developmental Biology Research
Internal/Organ Defects (vasculature)

Hematological Research
Hematopoietic Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

References

Selected Reference(s)

Shalaby F; Rossant J; Yamaguchi TP; Gertsenstein M; Wu XF; Breitman ML; Schuh AC. 1995. Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376(6535):62-6. [PubMed: 7596435]  [MGI Ref ID J:26846]

Additional References

Price and Supply Information

Strain Name: B6.129-Kdrtm1Jrt/J
Stock Number: 002938

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)
Cryorecovery Fee $1900.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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