Strain Name:

B6.129S4-Il2ratm1Dw/J

Stock Number:

002952

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Availability:

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Mice homozygous for Il2ratm1Dw are are characterized by a lymphoproliferative disorder, hemolytic anemia, and an inflammatory bowel disease beginning at approximately 9 weeks of age.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S4 via J1 ES cell line
GenerationN6+N2F4 (10-DEC-13)
Generation Definitions
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Description
Young mice homozygous for the Il2ratm1Dw knock-out mutation have phenotypically normal development of T and B cells. Adults develop massive enlargement of peripheral lymphoid organs associated with polyclonal T and B cell expansion resulting in autoimmune disorders, including hemolytic anemia and inflammatory bowel disease beginning at approximately 9 weeks of age. The knocked-out gene is also known as p55 gene chain, Ly43, or CD25.

Development
This strain was developed in the laboratory of Dr. Dennis Willerford, Children's Hospital, Boston. A neomycin resistance cassette replaced a 5.5 kb segment of the gene, containing exons 2 and 3, which encode for the binding site of the protein. Correctly targeted, transfected J1 ES cells were injected into C57BL/6 blastocysts. The strain has been backcrossed for 7 generations to C57BL/6J before being maintained by inbreeding.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Il2ratm1Dw allele
002462   B6;129S4-Il2ratm1Dw/J
View Strains carrying   Il2ratm1Dw     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- No similarity to the expected human disease phenotype was found. One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of these genes, but the phenotype did not resemble the disease.
Interleukin 2 Receptor, Alpha, Deficiency of
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Diabetes Mellitus, Insulin-Dependent, 10; IDDM10   (IL2RA)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Il2ratm1Dw/Il2ratm1Dw

        B6.129S4-Il2ratm1Dw
  • mortality/aging
  • abnormal induced morbidity/mortality   (MGI Ref ID J:125748)
  • immune system phenotype
  • abnormal T cell activation
    • over 80% of the T cells from bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit an activated phenotype   (MGI Ref ID J:125748)
  • abnormal regulatory T cell physiology
    • bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) die within 40-50 days of autoimmune disorders   (MGI Ref ID J:125748)
    • however, when bone marrow chimeras receive 105 CD25-positive CD4-positive wild-type T cells two weeks after reconstitution they live past six months of age without any symptoms of autoimmune disorder   (MGI Ref ID J:125748)
    • premature death can also be prevented by including 50% normal or Il2tm1Hor bone marrow during reconstitution   (MGI Ref ID J:125748)
  • lymphoid hyperplasia
    • some bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) have enlarged lymph nodes containing up to 3 x 108 lymphocytes   (MGI Ref ID J:125748)
  • hematopoietic system phenotype
  • abnormal T cell activation
    • over 80% of the T cells from bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit an activated phenotype   (MGI Ref ID J:125748)
  • abnormal regulatory T cell physiology
    • bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) die within 40-50 days of autoimmune disorders   (MGI Ref ID J:125748)
    • however, when bone marrow chimeras receive 105 CD25-positive CD4-positive wild-type T cells two weeks after reconstitution they live past six months of age without any symptoms of autoimmune disorder   (MGI Ref ID J:125748)
    • premature death can also be prevented by including 50% normal or Il2tm1Hor bone marrow during reconstitution   (MGI Ref ID J:125748)
  • anemia
    • bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit anemia   (MGI Ref ID J:125748)
  • growth/size/body phenotype
  • decreased body size
    • bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit decreased body weight due to an autoimmune disorder   (MGI Ref ID J:125748)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Il2ratm1Dw/Il2ratm1Dw

        involves: 129S4/SvJae * C57BL/6
  • mortality/aging
  • premature death
    • about 25% die between 8 and 20 weeks of age with severe anemia   (MGI Ref ID J:64279)
  • hematopoietic system phenotype
  • abnormal B cell physiology   (MGI Ref ID J:64279)
    • increased IgA level
      • 5- to 30-fold increase in IgA   (MGI Ref ID J:64279)
    • increased IgG level
      • 5- to 30-fold increase in IgG1, IgG2a, and IgG2b   (MGI Ref ID J:64279)
      • increased IgG1 level   (MGI Ref ID J:64279)
      • increased IgG2a level   (MGI Ref ID J:64279)
      • increased IgG2b level   (MGI Ref ID J:64279)
  • abnormal T cell morphology
    • T cells are slightly larger than in wild-type in adults, however T and B cell development is normal up to 4 weeks after birth   (MGI Ref ID J:64279)
    • increased memory T cell number
      • increased numbers of cells bearing marker for memory cells   (MGI Ref ID J:64279)
  • abnormal T cell physiology
    • proliferative responses to CD3 are reduced in T cells   (MGI Ref ID J:64279)
    • peripheral deletion of staphylococcal enterotoxin B (SEB)-reactive T cells is impaired   (MGI Ref ID J:64279)
  • decreased hematocrit   (MGI Ref ID J:64279)
  • enlarged spleen
    • develop enlargement of the spleen between 4 and 6 weeks of age   (MGI Ref ID J:64279)
  • hemolytic anemia
    • develops as mice age   (MGI Ref ID J:64279)
  • increased lymphocyte cell number
    • polyclonal expansion of lymphocytes beginning between 4 and 6 weeks of age   (MGI Ref ID J:64279)
    • increased memory T cell number
      • increased numbers of cells bearing marker for memory cells   (MGI Ref ID J:64279)
  • immune system phenotype
  • abnormal B cell physiology   (MGI Ref ID J:64279)
    • increased IgA level
      • 5- to 30-fold increase in IgA   (MGI Ref ID J:64279)
    • increased IgG level
      • 5- to 30-fold increase in IgG1, IgG2a, and IgG2b   (MGI Ref ID J:64279)
      • increased IgG1 level   (MGI Ref ID J:64279)
      • increased IgG2a level   (MGI Ref ID J:64279)
      • increased IgG2b level   (MGI Ref ID J:64279)
  • abnormal T cell morphology
    • T cells are slightly larger than in wild-type in adults, however T and B cell development is normal up to 4 weeks after birth   (MGI Ref ID J:64279)
    • increased memory T cell number
      • increased numbers of cells bearing marker for memory cells   (MGI Ref ID J:64279)
  • abnormal T cell physiology
    • proliferative responses to CD3 are reduced in T cells   (MGI Ref ID J:64279)
    • peripheral deletion of staphylococcal enterotoxin B (SEB)-reactive T cells is impaired   (MGI Ref ID J:64279)
  • abnormal lymph organ size   (MGI Ref ID J:64279)
    • enlarged lymph nodes
      • develop enlargement of the lymph nodes between 4 and 6 weeks of age   (MGI Ref ID J:64279)
    • enlarged spleen
      • develop enlargement of the spleen between 4 and 6 weeks of age   (MGI Ref ID J:64279)
  • colitis
    • starting at 12-16 weeks of age, develop severe bowel inflammation that is confined to the colon   (MGI Ref ID J:64279)
  • increased lymphocyte cell number
    • polyclonal expansion of lymphocytes beginning between 4 and 6 weeks of age   (MGI Ref ID J:64279)
    • increased memory T cell number
      • increased numbers of cells bearing marker for memory cells   (MGI Ref ID J:64279)
  • digestive/alimentary phenotype
  • colitis
    • starting at 12-16 weeks of age, develop severe bowel inflammation that is confined to the colon   (MGI Ref ID J:64279)
  • diarrhea
    • starts at 12-16 weeks of age as mice develop inflammatory bowel disease   (MGI Ref ID J:64279)
  • growth/size/body phenotype
  • cachexia
    • starts at 12-16 weeks of age as mice develop inflammatory bowel disease   (MGI Ref ID J:64279)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il2ratm1Dw related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      hemolytic anemia
Growth Factors/Receptors/Cytokines
Immunodeficiency
      Inflammatory bowel disease
Inflammation
      Inflammatory bowel disease

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il2ratm1Dw
Allele Name targeted mutation 1, Dennis M Willerford
Allele Type Targeted (Null/Knockout)
Common Name(s) CD25-; CD25KO; IL-2Ralpha KO; IL-2Ralpha-; IL2Ralpha-;
Mutation Made ByDr. Dennis Willerford,   Children's Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Il2ra, interleukin 2 receptor, alpha chain
Chromosome 2
Gene Common Name(s) CD25; IDDM10; IL-2R alpha chain; IL2R; Il2r; Ly-43; TCGFR; interleukin 2 receptor; lymphocyte antigen 43;
General Note Phenotypic Similarity to Human Syndrome: Inflammatory Bowel Disease (J:64279).
Molecular Note A neomycin resistance cassette replaced a 5.5 kb segment of the gene, containing exons 2 and 3, which encode for the binding site of the protein. [MGI Ref ID J:64279]

Genotyping

Genotyping Information

Genotyping Protocols

Il2ratm1Dw, Melt Curve Analysis
Il2ratm1Dw, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Willerford DM; Chen J; Ferry JA; Davidson L; Ma A; Alt FW. 1995. Interleukin-2 receptor alpha chain regulates the size and content of the peripheral lymphoid compartment. Immunity 3(4):521-30. [PubMed: 7584142]  [MGI Ref ID J:64279]

Additional References

Antony PA; Paulos CM; Ahmadzadeh M; Akpinarli A; Palmer DC; Sato N; Kaiser A; Hinrichs CS; Klebanoff CA; Tagaya Y; Restifo NP. 2006. Interleukin-2-dependent mechanisms of tolerance and immunity in vivo. J Immunol 176(9):5255-66. [PubMed: 16621991]  [MGI Ref ID J:115150]

Poussier P; Ning T; Chen J; Banerjee D; Julius M. 2000. Intestinal inflammation observed in IL-2R/IL-2 mutant mice is associated with impaired intestinal T lymphopoiesis [see comments] Gastroenterology 118(5):880-91. [PubMed: 10784587]  [MGI Ref ID J:61842]

Il2ratm1Dw related

Almeida AR; Legrand N; Papiernik M; Freitas AA. 2002. Homeostasis of peripheral CD4+ T cells: IL-2R alpha and IL-2 shape a population of regulatory cells that controls CD4+ T cell numbers. J Immunol 169(9):4850-60. [PubMed: 12391195]  [MGI Ref ID J:125748]

Amado IF; Berges J; Luther RJ; Mailhe MP; Garcia S; Bandeira A; Weaver C; Liston A; Freitas AA. 2013. IL-2 coordinates IL-2-producing and regulatory T cell interplay. J Exp Med 210(12):2707-20. [PubMed: 24249704]  [MGI Ref ID J:208088]

Antony PA; Paulos CM; Ahmadzadeh M; Akpinarli A; Palmer DC; Sato N; Kaiser A; Hinrichs CS; Klebanoff CA; Tagaya Y; Restifo NP. 2006. Interleukin-2-dependent mechanisms of tolerance and immunity in vivo. J Immunol 176(9):5255-66. [PubMed: 16621991]  [MGI Ref ID J:115150]

Bachmann MF; Wolint P; Walton S; Schwarz K; Oxenius A. 2007. Differential role of IL-2R signaling for CD8+ T cell responses in acute and chronic viral infections. Eur J Immunol 37(6):1502-12. [PubMed: 17492805]  [MGI Ref ID J:123518]

Ballesteros-Tato A; Leon B; Graf BA; Moquin A; Adams PS; Lund FE; Randall TD. 2012. Interleukin-2 inhibits germinal center formation by limiting T follicular helper cell differentiation. Immunity 36(5):847-56. [PubMed: 22464171]  [MGI Ref ID J:187331]

Boone DL; Dassopoulos T; Chai S; Chien M; Lodolce J; Ma A. 2003. Fas is not essential for lamina propria T lymphocyte homeostasis. Am J Physiol Gastrointest Liver Physiol 285(2):G382-8. [PubMed: 12702495]  [MGI Ref ID J:84826]

Burchill MA; Yang J; Vogtenhuber C; Blazar BR; Farrar MA. 2007. IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells. J Immunol 178(1):280-90. [PubMed: 17182565]  [MGI Ref ID J:141932]

Cho JH; Boyman O; Kim HO; Hahm B; Rubinstein MP; Ramsey C; Kim DM; Surh CD; Sprent J. 2007. An intense form of homeostatic proliferation of naive CD8+ cells driven by IL-2. J Exp Med 204(8):1787-801. [PubMed: 17664294]  [MGI Ref ID J:125917]

Cho JH; Kim HO; Kim KS; Yang DH; Surh CD; Sprent J. 2013. Unique features of naive CD8+ T cell activation by IL-2. J Immunol 191(11):5559-73. [PubMed: 24166977]  [MGI Ref ID J:207012]

D'Cruz LM; Klein L. 2005. Development and function of agonist-induced CD25+Foxp3+ regulatory T cells in the absence of interleukin 2 signaling. Nat Immunol 6(11):1152-9. [PubMed: 16227983]  [MGI Ref ID J:112603]

D'Souza WN; Lefrancois L. 2003. IL-2 is not required for the initiation of CD8 T cell cycling but sustains expansion. J Immunol 171(11):5727-35. [PubMed: 14634080]  [MGI Ref ID J:117985]

Dooms H; Wolslegel K; Lin P; Abbas AK. 2007. Interleukin-2 enhances CD4+ T cell memory by promoting the generation of IL-7R alpha-expressing cells. J Exp Med 204(3):547-57. [PubMed: 17312008]  [MGI Ref ID J:125364]

Fontenot JD; Rasmussen JP; Gavin MA; Rudensky AY. 2005. A function for interleukin 2 in Foxp3-expressing regulatory T cells. Nat Immunol 6(11):1142-51. [PubMed: 16227984]  [MGI Ref ID J:112602]

Fujimura K; Oyamada A; Iwamoto Y; Yoshikai Y; Yamada H. 2013. CD4 T cell-intrinsic IL-2 signaling differentially affects Th1 and Th17 development. J Leukoc Biol 94(2):271-9. [PubMed: 23715742]  [MGI Ref ID J:203220]

Halim TY; Maclaren A; Romanish MT; Gold MJ; McNagny KM; Takei F. 2012. Retinoic-Acid-receptor-related orphan nuclear receptor alpha is required for natural helper cell development and allergic inflammation. Immunity 37(3):463-74. [PubMed: 22981535]  [MGI Ref ID J:187662]

Hayashi RJ; Kanagawa O. 2006. Requirement of high-affinity IL-2-IL-2R interaction for T cell anergy induction. Int Immunol 18(5):645-51. [PubMed: 16574670]  [MGI Ref ID J:108552]

Hu MG; Deshpande A; Schlichting N; Hinds EA; Mao C; Dose M; Hu GF; Van Etten RA; Gounari F; Hinds PW. 2011. CDK6 kinase activity is required for thymocyte development. Blood 117(23):6120-31. [PubMed: 21508411]  [MGI Ref ID J:174697]

Hu Z; Zhang W; Usherwood EJ. 2013. Regulatory CD8+ T cells associated with erosion of immune surveillance in persistent virus infection suppress in vitro and have a reversible proliferative defect. J Immunol 191(1):312-22. [PubMed: 23733872]  [MGI Ref ID J:205357]

Hua L; Yao S; Pham D; Jiang L; Wright J; Sawant D; Dent AL; Braciale TJ; Kaplan MH; Sun J. 2013. Cytokine-dependent induction of CD4+ T cells with cytotoxic potential during influenza virus infection. J Virol 87(21):11884-93. [PubMed: 23986597]  [MGI Ref ID J:203928]

Johnston RJ; Choi YS; Diamond JA; Yang JA; Crotty S. 2012. STAT5 is a potent negative regulator of TFH cell differentiation. J Exp Med 209(2):243-50. [PubMed: 22271576]  [MGI Ref ID J:181874]

Kobayashi CI; Takubo K; Kobayashi H; Nakamura-Ishizu A; Honda H; Kataoka K; Kumano K; Akiyama H; Sudo T; Kurokawa M; Suda T. 2014. The IL-2/CD25 axis maintains distinct subsets of chronic myeloid leukemia-initiating cells. Blood 123(16):2540-9. [PubMed: 24574458]  [MGI Ref ID J:210745]

Krieg C; Letourneau S; Pantaleo G; Boyman O. 2010. Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells. Proc Natl Acad Sci U S A 107(26):11906-11. [PubMed: 20547866]  [MGI Ref ID J:161365]

Kronin V; Vremec D; Shortman K. 1998. Does the IL-2 receptor alpha chain induced on dendritic cells have a biological function? Int Immunol 10(2):237-40. [PubMed: 9533452]  [MGI Ref ID J:110430]

Kung JT; Beller D; Ju ST. 1998. Lymphokine regulation of activation-induced apoptosis in T cells of IL-2 and IL-2R beta knockout mice. Cell Immunol 185(2):158-63. [PubMed: 9636694]  [MGI Ref ID J:114259]

Leon B; Bradley JE; Lund FE; Randall TD; Ballesteros-Tato A. 2014. FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability. Nat Commun 5:3495. [PubMed: 24633065]  [MGI Ref ID J:210317]

Mitchell DM; Ravkov EV; Williams MA. 2010. Distinct roles for IL-2 and IL-15 in the differentiation and survival of CD8+ effector and memory T cells. J Immunol 184(12):6719-30. [PubMed: 20483725]  [MGI Ref ID J:161153]

Nelson BH. 2004. IL-2, regulatory T cells, and tolerance. J Immunol 172(7):3983-8. [PubMed: 15034008]  [MGI Ref ID J:88679]

Obar JJ; Lefrancois L. 2010. Early signals during CD8(+) T cell priming regulate the generation of central memory cells. J Immunol 185(1):263-72. [PubMed: 20519649]  [MGI Ref ID J:161623]

Obar JJ; Molloy MJ; Jellison ER; Stoklasek TA; Zhang W; Usherwood EJ; Lefrancois L. 2010. CD4+ T cell regulation of CD25 expression controls development of short-lived effector CD8+ T cells in primary and secondary responses. Proc Natl Acad Sci U S A 107(1):193-8. [PubMed: 19966302]  [MGI Ref ID J:156498]

Papiernik M; de Moraes ML; Pontoux C; Vasseur F; Penit C. 1998. Regulatory CD4 T cells: expression of IL-2R alpha chain, resistance to clonal deletion and IL-2 dependency. Int Immunol 10(4):371-8. [PubMed: 9620592]  [MGI Ref ID J:110541]

Pipkin ME; Sacks JA; Cruz-Guilloty F; Lichtenheld MG; Bevan MJ; Rao A. 2010. Interleukin-2 and inflammation induce distinct transcriptional programs that promote the differentiation of effector cytolytic T cells. Immunity 32(1):79-90. [PubMed: 20096607]  [MGI Ref ID J:157692]

Pontoux C; Banz A; Papiernik M. 2002. Natural CD4 CD25(+) regulatory T cells control the burst of superantigen-induced cytokine production: the role of IL-10. Int Immunol 14(2):233-9. [PubMed: 11809742]  [MGI Ref ID J:113510]

Poussier P; Ning T; Chen J; Banerjee D; Julius M. 2000. Intestinal inflammation observed in IL-2R/IL-2 mutant mice is associated with impaired intestinal T lymphopoiesis [see comments] Gastroenterology 118(5):880-91. [PubMed: 10784587]  [MGI Ref ID J:61842]

Rahimy E; Pitcher JD 3rd; Pangelinan SB; Chen W; Farley WJ; Niederkorn JY; Stern ME; Li DQ; Pflugfelder SC; De Paiva CS. 2010. Spontaneous autoimmune dacryoadenitis in aged CD25KO mice. Am J Pathol 177(2):744-53. [PubMed: 20566743]  [MGI Ref ID J:163396]

Ramsey C; Rubinstein MP; Kim DM; Cho JH; Sprent J; Surh CD. 2008. The lymphopenic environment of CD132 (common gamma-chain)-deficient hosts elicits rapid homeostatic proliferation of naive T cells via IL-15. J Immunol 180(8):5320-6. [PubMed: 18390713]  [MGI Ref ID J:134238]

Redmond WL; Triplett T; Floyd K; Weinberg AD. 2012. Dual anti-OX40/IL-2 therapy augments tumor immunotherapy via IL-2R-mediated regulation of OX40 expression. PLoS One 7(4):e34467. [PubMed: 22496812]  [MGI Ref ID J:187106]

Saint Fleur S; Hoshino A; Kondo K; Egawa T; Fujii H. 2009. Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon. Blood 114(7):1355-65. [PubMed: 19528538]  [MGI Ref ID J:151722]

Sawamukai N; Satake A; Schmidt AM; Lamborn IT; Ojha P; Tanaka Y; Kambayashi T. 2012. Cell-autonomous role of TGFbeta and IL-2 receptors in CD4(+) and CD8(+) inducible regulatory T-cell generation during GVHD. Blood 119(23):5575-83. [PubMed: 22496155]  [MGI Ref ID J:188089]

Scumpia PO; Delano MJ; Kelly KM; O'Malley KA; Efron PA; McAuliffe PF; Brusko T; Ungaro R; Barker T; Wynn JL; Atkinson MA; Reeves WH; Salzler MJ; Moldawer LL. 2006. Increased natural CD4+CD25+ regulatory T cells and their suppressor activity do not contribute to mortality in murine polymicrobial sepsis. J Immunol 177(11):7943-9. [PubMed: 17114466]  [MGI Ref ID J:140690]

Sepulveda H; Cerwenka A; Morgan T; Dutton RW. 1999. CD28, IL-2-independent costimulatory pathways for CD8 T lymphocyte activation. J Immunol 163(3):1133-42. [PubMed: 10415007]  [MGI Ref ID J:118773]

Sharma R; Bagavant H; Jarjour WN; Sung SS; Ju ST. 2005. The role of Fas in the immune system biology of IL-2R alpha knockout mice: interplay among regulatory T cells, inflammation, hemopoiesis, and apoptosis. J Immunol 175(3):1965-73. [PubMed: 16034141]  [MGI Ref ID J:107264]

Sharma R; Zheng L; Deshmukh US; Jarjour WN; Sung SS; Fu SM; Ju ST. 2007. A regulatory T cell-dependent novel function of CD25 (IL-2Ralpha) controlling memory CD8(+) T cell homeostasis. J Immunol 178(3):1251-5. [PubMed: 17237369]  [MGI Ref ID J:143666]

Sharma R; Zheng L; Guo X; Fu SM; Ju ST; Jarjour WN. 2006. Novel animal models for Sjogren's syndrome: expression and transfer of salivary gland dysfunction from regulatory T cell-deficient mice. J Autoimmun 27(4):289-96. [PubMed: 17207605]  [MGI Ref ID J:125129]

Shibata K; Yamada H; Nakamura R; Sun X; Itsumi M; Yoshikai Y. 2008. Identification of CD25+ gammadelta T cells as fetal thymus-derived naturally occurring IL-17 producers. J Immunol 181(9):5940-7. [PubMed: 18941182]  [MGI Ref ID J:140747]

Soper DM; Kasprowicz DJ; Ziegler SF. 2007. IL-2Rbeta links IL-2R signaling with Foxp3 expression. Eur J Immunol 37(7):1817-26. [PubMed: 17559173]  [MGI Ref ID J:123613]

Tortola L; Yadava K; Bachmann MF; Muller C; Kisielow J; Kopf M. 2010. IL-21 induces death of marginal zone B cells during chronic inflammation. Blood 116(24):5200-7. [PubMed: 20826721]  [MGI Ref ID J:167415]

Van Parijs L; Biuckians A; Ibragimov A; Alt FW; Willerford DM; Abbas AK. 1997. Functional responses and apoptosis of CD25 (IL-2R alpha)-deficient T cells expressing a transgenic antigen receptor. J Immunol 158(8):3738-45. [PubMed: 9103438]  [MGI Ref ID J:110677]

Walton SM; Torti N; Mandaric S; Oxenius A. 2011. T-cell help permits memory CD8(+) T-cell inflation during cytomegalovirus latency. Eur J Immunol 41(8):2248-59. [PubMed: 21590767]  [MGI Ref ID J:176830]

Wiesel M; Joller N; Ehlert AK; Crouse J; Sporri R; Bachmann MF; Oxenius A. 2010. Th cells act via two synergistic pathways to promote antiviral CD8+ T cell responses. J Immunol 185(9):5188-97. [PubMed: 20881183]  [MGI Ref ID J:165190]

Williams CA; Murray SE; Weinberg AD; Parker DC. 2007. OX40-mediated differentiation to effector function requires IL-2 receptor signaling but not CD28, CD40, IL-12Rbeta2, or T-bet. J Immunol 178(12):7694-702. [PubMed: 17548606]  [MGI Ref ID J:148588]

Williams MA; Tyznik AJ; Bevan MJ. 2006. Interleukin-2 signals during priming are required for secondary expansion of CD8+ memory T cells. Nature 441(7095):890-3. [PubMed: 16778891]  [MGI Ref ID J:110049]

Wilson EB; Livingstone AM. 2008. Cutting edge: CD4+ T cell-derived IL-2 is essential for help-dependent primary CD8+ T cell responses. J Immunol 181(11):7445-8. [PubMed: 19017930]  [MGI Ref ID J:142211]

Yang CY; Best JA; Knell J; Yang E; Sheridan AD; Jesionek AK; Li HS; Rivera RR; Lind KC; D'Cruz LM; Watowich SS; Murre C; Goldrath AW. 2011. The transcriptional regulators Id2 and Id3 control the formation of distinct memory CD8+ T cell subsets. Nat Immunol 12(12):1221-9. [PubMed: 22057289]  [MGI Ref ID J:179006]

Zheng L; Sharma R; Kung JT; Deshmukh US; Jarjour WN; Fu SM; Ju ST. 2008. Pervasive and stochastic changes in the TCR repertoire of regulatory T-cell-deficient mice. Int Immunol 20(4):517-23. [PubMed: 18310063]  [MGI Ref ID J:133521]

Zheng L; Trageser CL; Willerford DM; Lenardo MJ. 1998. T cell growth cytokines cause the superinduction of molecules mediating antigen-induced T lymphocyte death. J Immunol 160(2):763-9. [PubMed: 9551911]  [MGI Ref ID J:111099]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Mating SystemHeterozygote x Heterozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHeterozygous for Il2ratm1Dw  
$239.00Female or MaleHomozygous for Il2ratm1Dw  
Price per Pair (US dollars $)Pair Genotype
$478.00Heterozygous for Il2ratm1Dw x Heterozygous for Il2ratm1Dw  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHeterozygous for Il2ratm1Dw  
$310.70Female or MaleHomozygous for Il2ratm1Dw  
Price per Pair (US dollars $)Pair Genotype
$621.40Heterozygous for Il2ratm1Dw x Heterozygous for Il2ratm1Dw  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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