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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 08-MAY-09 Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Appearance
albino
Related Genotype: Tyrc/TyrcImportant Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.Description
Mice carrying the (MMTVTGFA)254Rjc transgene are viable and fertile, but lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgenic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females transgene expression was localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also revealed some stromal staining in the hyperplastic areas. Egfr mRNA expression was also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The B6D2-TgN(MMTVTGFA)254Rjc line is notable for sebaceous gland hyperplasia, which occurs in both male and female mice. The hyperplasia was evidenced as multiple raised, white plaques and nodules on the abdominal skin. No dysplasia or sebaceous cell carcinoma was observed. High expression of TGFa mRNA and TGFa protein was detected in the areas of sebaceous hyperplasia.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Pde6brd1 allele
View Strains carrying Pde6brd1 (74 strains)
002459 B6D2-Tg(MMTVTGFA)254Rjc/J
004297 B6.CXB1-Pde6brd10/J 003647 B6EiC3Sn.BLiAF1 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002421 FVB/N-Tg(MtTGFA)100Lmb/J 002422 STOCK Tg(MtTGFA)42Lmb/J 003722 SW.Cg-Tg(MtTGFA)42Lmb/J
004997 B6.Cg-Tg(MMTV-TGFBR2)7Hlm/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002375 B6;D2-Tg(MMTVTGFB1)46Hlm/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 004363 FVB.Cg-Tg(MMTV-vHaras)SH1Led/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J 002374 FVB/N-Tg(MMTV-PyVT)634Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J 002933 FVB/NJ-Tg(MMTVTGFB1)46Hlm/J 003690 STOCK Tg(MMTV-Cdc37)1Stp/J 003337 STOCK Tg(MMTV-PIP)1Shu/J 003551 STOCK Tg(MMTV-cre)1Mam/J 003553 STOCK Tg(MMTV-cre)4Mam/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(MMTVTGFA)254Rjc/0
involves: C57BL/6 * DBA/2
- endocrine/exocrine gland phenotype
- abnormal mammary gland morphology (MGI Ref ID J:28453)
- at 2.5 months of age, the virgin female founder displayed duct abnormalities but little alveolar development
- mammary gland hyperplasia (MGI Ref ID J:72085)
- female mice develop cystic and solid hyperplasias
- enlarged sebaceous gland (MGI Ref ID J:72085)
- mice exhibit sebaceous gland hyperplasia
- tumorigenesis
- mammary adenocarcinoma (MGI Ref ID J:72085)
- behavior/neurological phenotype
- abnormal nursing (MGI Ref ID J:72085)
- females cannot nurse her young
- skin/coat/nails phenotype
- abnormal skin condition (MGI Ref ID J:72085)
- mice exhibit multiple, raised, white plaques and nodules on the abdominal skin
- reproductive system phenotype
- abnormal mammary gland morphology (MGI Ref ID J:28453)
- at 2.5 months of age, the virgin female founder displayed duct abnormalities but little alveolar development
- mammary gland hyperplasia (MGI Ref ID J:72085)
- female mice develop cystic and solid hyperplasias
This mouse can be used to support research in many areas including:
Pde6brd1 relatedCancer Research
Increased Tumor Incidence
Mammary Gland Tumors
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(MMTVTGFA)254Rjc | ||
|---|---|---|---|
| Allele Name | transgene insertion 254, Robert J Coffey | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Robert Coffey, Vanderbilt University Medical Center | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | TGFA, transforming growth factor, alpha, human | ||
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV | ||
| Molecular Note | The transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. [MGI Ref ID J:28453] | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | Pdebrd1; rd; rd-1; rd1; rodless retina; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(MMTVTGFA), Standard PCR
Tg(MMTVTGFA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Matsui Y; Halter SA; Holt JT; Hogan BL; Coffey RJ. 1990. Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice. Cell 61(6):1147-55. [PubMed: 2161707] [MGI Ref ID J:28453]
Additional References
Tg(MMTVTGFA)254Rjc relatedCleary MP; Grande JP; Maihle NJ. 2004. Effect of high fat diet on body weight and mammary tumor latency in MMTV-TGF-alpha mice. Int J Obes Relat Metab Disord 28(8):956-62. [PubMed: 15254485] [MGI Ref ID J:92145]
Cleary MP; Hu X; Grossmann ME; Juneja SC; Dogan S; Grande JP; Maihle NJ. 2007. Prevention of mammary tumorigenesis by intermittent caloric restriction: does caloric intake during refeeding modulate the response? Exp Biol Med (Maywood) 232(1):70-80. [PubMed: 17202587] [MGI Ref ID J:129353]
Cleary MP; Jacobson MK; Phillips FC; Getzin SC; Grande JP; Maihle NJ. 2002. Weight-cycling decreases incidence and increases latency of mammary tumors to a greater extent than does chronic caloric restriction in mouse mammary tumor virus-transforming growth factor-alpha female mice. Cancer Epidemiol Biomarkers Prev 11(9):836-43. [PubMed: 12223427] [MGI Ref ID J:79070]
Cleary MP; Juneja SC; Phillips FC; Hu X; Grande JP; Maihle NJ. 2004. Leptin receptor-deficient MMTV-TGF-alpha/Lepr(db)Lepr(db) female mice do not develop oncogene-induced mammary tumors. Exp Biol Med (Maywood) 229(2):182-93. [PubMed: 14734797] [MGI Ref ID J:102011]
Cleary MP; Phillips FC; Getzin SC; Jacobson TL; Jacobson MK; Christensen TA; Juneja SC; Grande JP; Maihle NJ. 2003. Genetically obese MMTV-TGF-alpha/Lep(ob)Lep(ob) female mice do not develop mammary tumors. Breast Cancer Res Treat 77(3):205-15. [PubMed: 12602920] [MGI Ref ID J:81933]
Dogan S; Hu X; Zhang Y; Maihle NJ; Grande JP; Cleary MP. 2007. Effects of high-fat diet and/or body weight on mammary tumor leptin and apoptosis signaling pathways in MMTV-TGF-alpha mice. Breast Cancer Res 9(6):R91. [PubMed: 18162139] [MGI Ref ID J:133411]
Halter SA; Dempsey P; Matsui Y; Stokes MK; Graves-Deal R; Hogan BL; Coffey RJ. 1992. Distinctive patterns of hyperplasia in transgenic mice with mouse mammary tumor virus transforming growth factor-alpha. Characterization of mammary gland and skin proliferations. Am J Pathol 140(5):1131-46. [PubMed: 1316084] [MGI Ref ID J:72085]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Mating System +/+ sibling x Hemizygote (Female x Male) 08-MAY-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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| Important Note | |
| This strain is homozygous for the retinal degeneration allele Pde6brd1. | |
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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