Strain Name:

B6.129S4-Drd3tm1Dac/J

Stock Number:

002958

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Availability:

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Other products are available, see Purchasing Information for Cryopreserved Embryos

These DRD3 mice may be useful for studying dopamine autoreceptor inhibitory control in dopaminergic neurotransmission.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   30-APR-14
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S4 via J1 ES cell line
Generation?+N8p (17-SEP-13)
Generation Definitions
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Description
D3 receptors are G-protein coupled receptors localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions by inhibition of adenylyl cyclase activity. Dopamine (DA) stimulation of presynaptic D3 receptors induces a negative feedback regulation that reduces DA neuron firing, DA synthesis and DA release. Defects in DA neurons have been associated with Parkinson's disease, schizophrenia, attention-deficit and hyperactivity disorder, and compulsive drug abuse. D3 receptors are also expressed in renal proximal tubules and juxtaglomerular cells and may be responsible for the dopamine-mediated decrease in renin secretion in these cells. Homozygous are viable and fertile. Dopamine antagonist binding studies indicate the absence of D3 receptors in homozygotes and a greatly reduced number in heterozygotes. These DRD3 KO mice display enhanced cocaine self-administration and enhanced motivation for cocaine-taking and cocaine-seeking behavior. They also exhibit an increase in locomotor activity, increase in exploratory behavior, and decrease in grooming. Behavioral alterations were less pronounced in heterozygotes. When treated with cocaine they exhibit reduced hyperactivity and increased head bobbing. Both systolic and diastolic blood pressures were approximately 20 mmHg higher in heterozygotes and homozygotes compared to wild-type mice.

Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette and 30 non-endogenous residues (STOP) into exon 2 of the dopamine receptor D3 (Drd3) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were imported on a mixed B6 x 129S4 background as Stock No. 002425. Upon arrival at The Jackson Laboratory, these mice were backcrossed to C57BL/6J mice (Stock No. 000664) to establish a congenic colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Drd3tm1Dac allele
002425   B6;129S4-Drd3tm1Dac/J
View Strains carrying   Drd3tm1Dac     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Schizophrenia; SCZD   (DRD3)
Tremor, Hereditary Essential, 1; ETM1   (DRD3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Drd3tm1Dac/Drd3tm1Dac

        B6.129S4-Drd3tm1Dac/J
  • behavior/neurological phenotype
  • abnormal motor coordination/ balance
    • on day 2, mice exhibit improved performance on a rotarod compared with wild-type mice   (MGI Ref ID J:102600)
  • abnormal reflex
    • mice exhibit more large-reflex amplitudes for both monosynaptic 'stretch' reflex (MSR) and longer-latency reflex responses compared with wild-type mice   (MGI Ref ID J:96803)
    • mice treated with 1 or 5 um dopamine exhibit led to an increase in MSR amplitude and reflex strength unlike in similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with a D3 receptor antagonist do not exhibit an increase in monosynaptic reflex strength unlike in similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with D2 receptor agonists fail to exhibit a decrease in monosynaptic reflex strength compared with similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with pergolide do not exhibit a difference in monosynaptic and longer-latency reflex amplitudes unlike in similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with GR 103691 exhibit shorter monosynaptic than longer-latency reflex amplitude unlike in wild-type mice   (MGI Ref ID J:96803)
  • decreased anxiety-related response
    • mice exhibit increased entry into open arms in an elevated plus maze compared with wild-type mice   (MGI Ref ID J:102600)
  • hyperactivity
    • in an open field compared with wild-type mice   (MGI Ref ID J:102600)
  • increased vertical activity   (MGI Ref ID J:102600)
  • homeostasis/metabolism phenotype
  • abnormal physiological response to xenobiotic
    • mice treated with a D3 receptor antagonist do not exhibit an increase in monosynaptic reflex strength unlike in similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with D2 receptor agonists fail to exhibit a decrease in monosynaptic reflex strength compared with similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with pergolide do not exhibit a difference in monosynaptic and longer-latency reflex amplitudes unlike in similarly treated wild-type mice   (MGI Ref ID J:96803)
    • mice treated with GR 103691 exhibit shorter monosynaptic than longer-latency reflex amplitude unlike in wild-type mice   (MGI Ref ID J:96803)

Drd3tm1Dac/Drd3tm1Dac

        B6.129S4-Drd3tm1Dac
  • behavior/neurological phenotype
  • decreased startle reflex
    • mice exhibit reduced acoustic startle response amplitude compared with wild-type mice   (MGI Ref ID J:154428)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Drd3tm1Dac/Drd3+

        involves: 129S4/SvJae * C57BL/6
  • behavior/neurological phenotype
  • abnormal locomotor activation
    • during the first part of the test (increased early and decreased late) unlike wild-type mice   (MGI Ref ID J:96229)
    • hyperactivity
      • in the open field test, heterozygotes display hyperactivity, as shown by a 38% increase in locomotor activity (crossings) relative to wild-type mice   (MGI Ref ID J:32128)
      • during the first part of the test   (MGI Ref ID J:96229)
    • increased vertical activity
      • in the open field test, heterozygotes display 77% more rearing episodes than wild-type mice   (MGI Ref ID J:32128)
      • during the first part of the test   (MGI Ref ID J:96229)
  • decreased grooming behavior
    • heterozygotes tend to show reduced grooming behavior with a delayed onset; however, these differences are not statistically significant relative to wild-type grooming behavior   (MGI Ref ID J:32128)
  • increased exploration in new environment
    • heterozygotes exhibit increased exploratory behavior in the open field, with a degree of hyperactivity comparable to than of homozygotes during the last 11 min of a 15-min test   (MGI Ref ID J:32128)
  • cardiovascular system phenotype
  • increased systemic arterial diastolic blood pressure
    • after anesthesia with pentobarbital   (MGI Ref ID J:115136)
  • increased systemic arterial systolic blood pressure
    • after anesthesia with pentobarbital   (MGI Ref ID J:115136)
  • homeostasis/metabolism phenotype
  • increased renin activity   (MGI Ref ID J:115136)

Drd3tm1Dac/Drd3+

        involves: 129S4/SvJae * C57BL/6J
  • behavior/neurological phenotype
  • decreased anxiety-related response
    • mice exhibit moderately more time in the center of the open field and with the open arms of an elevated plus maze compared with wild-type mice   (MGI Ref ID J:44685)

Drd3tm1Dac/Drd3tm1Dac

        involves: 129S4/SvJae * C57BL/6
  • behavior/neurological phenotype
  • decreased grooming behavior
    • homozygotes tend to show reduced grooming behavior with a delayed onset; however, these differences are not statistically significant relative to wild-type grooming behavior   (MGI Ref ID J:32128)
  • hyperactivity
    • in the open field test, homozygotes display hyperactivity, as shown by a 57% increase in locomotor activity (crossings) relative to wild-type mice   (MGI Ref ID J:32128)
  • impaired behavioral response to morphine
    • mice exhibit decreased morphine-induced conditioned place preference (CPP) compared with similarly treated wild-type mice   (MGI Ref ID J:103701)
    • BP 897-treated mice fail to exhibit a decrease in morphine-induced CPP compared with similarly treated wild-type mice   (MGI Ref ID J:103701)
  • impaired conditioned place preference behavior
    • mice exhibit decreased morphine-induced conditioned place preference (CPP) compared with similarly treated wild-type mice   (MGI Ref ID J:103701)
    • BP 897-treated mice fail to exhibit a decrease in morphine-induced CPP compared with similarly treated wild-type mice   (MGI Ref ID J:103701)
  • increased exploration in new environment
    • homozygotes exhibit increased exploratory behavior in the open field test   (MGI Ref ID J:32128)
    • in contrast, normal gait, coordination and primitive reflexes remain intact   (MGI Ref ID J:32128)
  • increased vertical activity
    • in the open field test, homozygotes display 93% more rearing episodes than wild-type mice   (MGI Ref ID J:32128)
  • cardiovascular system phenotype
  • decreased systemic arterial systolic blood pressure
    • systolic blood pressure in losartan-treated mice remains depressed longer than similarly treated wild-type mice   (MGI Ref ID J:115136)
  • increased systemic arterial diastolic blood pressure
    • after anesthesia with pentobarbital   (MGI Ref ID J:115136)
  • increased systemic arterial systolic blood pressure
    • after anesthesia with pentobarbital   (MGI Ref ID J:115136)
  • homeostasis/metabolism phenotype
  • abnormal physiological response to xenobiotic
    • systolic blood pressure in losartan-treated mice remains depressed longer than similarly treated wild-type mice   (MGI Ref ID J:115136)
  • decreased urine sodium level
    • after acute saline loading   (MGI Ref ID J:115136)
  • increased renin activity
    • in the kidneys   (MGI Ref ID J:115136)
  • renal/urinary system phenotype
  • decreased urine flow rate
    • after acute saline loading, urine flow is decreased compared to in wild-type mice   (MGI Ref ID J:115136)
  • decreased urine sodium level
    • after acute saline loading   (MGI Ref ID J:115136)

Drd3tm1Dac/Drd3tm1Dac

        involves: 129S4/SvJae * C57BL/6J
  • behavior/neurological phenotype
  • decreased anxiety-related response
    • mice exhibit more time in the center of the open field and with the open arms of an elevated plus maze compared with wild-type mice   (MGI Ref ID J:44685)

Drd3tm1Dac/Drd3tm1Dac

        involves: 129S4/SvJae
  • behavior/neurological phenotype
  • abnormal behavioral response to addictive substance
    • mice treated with cocaine exhibit reduced hyperactivity and increased head bobbing compared with similarly treated wild-type mice   (MGI Ref ID J:103704)
    • enhanced behavioral response to alcohol
      • after a single injection of ethanol, mice exhibit reduced onset time to sleep and longer sleep time compared with similarly treated wild-type mice   (MGI Ref ID J:107932)
    • increased behavioral withdrawal response
      • 3 and 6 hours after discontinuation of ethanol treatment, mice exhibit increased levels of withdrawal scores compared with similarly treated wild-type mice   (MGI Ref ID J:107932)
      • however, withdrawal scores at 12 and 18 hours are normal   (MGI Ref ID J:107932)
  • decreased alcohol consumption
    • during chronic ethanol treatment   (MGI Ref ID J:107932)
  • head bobbing
    • cocaine-treated mice exhibit increased head bobbing compared with similarly treated wild-type mice   (MGI Ref ID J:103704)
  • hypoactivity
    • 5 days after cocaine treatment, mice exhibit less induced locomotion compared with similarly treated wild-type mice   (MGI Ref ID J:103704)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Hypertension

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Behavioral and Learning Defects
      high anxiety
Neurodevelopmental Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Parkinson's Disease
Receptor Defects
      dopamine receptor

Drd3tm1Dac related

Cardiovascular Research
Hypertension

Neurobiology Research
Behavioral and Learning Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Drd3tm1Dac
Allele Name targeted mutation 1, Domenico Accili
Allele Type Targeted (Null/Knockout)
Common Name(s) D3-; delta148(+30);
Mutation Made ByProf. Sara Fuchs,   National Institutes of Health
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Drd3, dopamine receptor D3
Chromosome 16
Gene Common Name(s) D3 receptor; D3DR; D3R; ETM1; FET1;
General Note Phenotypic Similarity to Human Syndrome: Restless Leg Syndrome (J:96803)
Molecular Note A neomycin selection cassette and sequence encoding 30 non-endogenous residues was inserted into exon 2. RT-PCR analysis on RNA derived from brain of homozygous mice demonstrated the absence of normal transcript. Mutant transcript was detected in both heterozygous and homozygous mutant mice. Binding assays on brain sections derived from homozygous mice confirmed that no functional protein was expressed. Notably, heterozygotes displayed a nearly complete loss of D3-specific binding, to a greater extentthan expected for a mutation affecting expression of one allele of the Drd3 gene, raising the possibility that this mutation acts in a dominant-negative fashion. [MGI Ref ID J:32128]

Genotyping

Genotyping Information

Genotyping Protocols

Drd3tm1Dac, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Accili D; Fishburn CS; Drago J; Steiner H; Lachowicz JE; Park BH; Gauda EB; Lee EJ; Cool MH; Sibley DR; Gerfen CR; Westphal H; Fuchs S. 1996. A targeted mutation of the D3 dopamine receptor gene is associated with hyperactivity in mice. Proc Natl Acad Sci U S A 93(5):1945-9. [PubMed: 8700864]  [MGI Ref ID J:32128]

Additional References

Narita M; Mizuo K; Mizoguchi H; Sakata M; Narita M; Tseng LF; Suzuki T. 2003. Molecular evidence for the functional role of dopamine D3 receptor in the morphine-induced rewarding effect and hyperlocomotion. J Neurosci 23(3):1006-12. [PubMed: 12574430]  [MGI Ref ID J:81915]

Steiner H; Fuchs S; Accili D. 1997. D3 dopamine receptor-deficient mouse: evidence for reduced anxiety. Physiol Behav 63(1):137-41. [PubMed: 9402626]  [MGI Ref ID J:44685]

Drd3tm1Dac related

Asico LD; Ladines C; Fuchs S; Accili D; Carey RM; Semeraro C; Pocchiari F; Felder RA; Eisner GM; Jose PA. 1998. Disruption of the dopamine D3 receptor gene produces renin-dependent hypertension. J Clin Invest 102(3):493-8. [PubMed: 9691085]  [MGI Ref ID J:115136]

Boulay D; Depoortere R; Rostene W; Perrault G; Sanger DJ. 1999. Dopamine D3 receptor agonists produce similar decreases in body temperature and locomotor activity in D3 knock-out and wild-type mice. Neuropharmacology 38(4):555-65. [PubMed: 10221759]  [MGI Ref ID J:54420]

Boyce-Rustay JM; Risinger FO. 2003. Dopamine D3 receptor knockout mice and the motivational effects of ethanol. Pharmacol Biochem Behav 75(2):373-9. [PubMed: 12873629]  [MGI Ref ID J:102433]

Bucolo C; Leggio GM; Maltese A; Castorina A; D'Agata V; Drago F. 2012. Dopamine- receptor modulates intraocular pressure: implications for glaucoma. Biochem Pharmacol 83(5):680-6. [PubMed: 22178719]  [MGI Ref ID J:181898]

Carta AR; Gerfen CR; Steiner H. 2000. Cocaine effects on gene regulation in the striatum and behavior: increased sensitivity in D3 dopamine receptor-deficient mice. Neuroreport 11(11):2395-9. [PubMed: 10943692]  [MGI Ref ID J:103704]

Castorina A; D'Amico AG; Scuderi S; Leggio GM; Drago F; D'Agata V. 2013. Dopamine D3 receptor deletion increases tissue plasminogen activator (tPA) activity in prefrontal cortex and hippocampus. Neuroscience 250:546-56. [PubMed: 23906635]  [MGI Ref ID J:207038]

Clemens S; Hochman S. 2004. Conversion of the modulatory actions of dopamine on spinal reflexes from depression to facilitation in D3 receptor knock-out mice. J Neurosci 24(50):11337-45. [PubMed: 15601940]  [MGI Ref ID J:96803]

Clemens S; Sawchuk MA; Hochman S. 2005. Reversal of the circadian expression of tyrosine-hydroxylase but not nitric oxide synthase levels in the spinal cord of dopamine D3 receptor knockout mice. Neuroscience 133(2):353-7. [PubMed: 15878801]  [MGI Ref ID J:104271]

Collo G; Bono F; Cavalleri L; Plebani L; Merlo Pich E; Millan MJ; Spano PF; Missale C. 2012. Pre-synaptic dopamine D(3) receptor mediates cocaine-induced structural plasticity in mesencephalic dopaminergic neurons via ERK and Akt pathways. J Neurochem 120(5):765-78. [PubMed: 22145570]  [MGI Ref ID J:182779]

Diaz J; Pilon C; Le Foll B; Gros C; Triller A; Schwartz JC; Sokoloff P. 2000. Dopamine D3 receptors expressed by all mesencephalic dopamine neurons J Neurosci 20(23):8677-84. [PubMed: 11102473]  [MGI Ref ID J:66145]

Dowling P; Klinker F; Stadelmann C; Hasan K; Paulus W; Liebetanz D. 2011. Dopamine D3 receptor specifically modulates motor and sensory symptoms in iron-deficient mice. J Neurosci 31(1):70-7. [PubMed: 21209191]  [MGI Ref ID J:167855]

Frances H; Le Foll B; Diaz J; Smirnova M; Sokoloff P. 2004. Role of DRD3 in morphine-induced conditioned place preference using drd3-knockout mice. Neuroreport 15(14):2245-9. [PubMed: 15371743]  [MGI Ref ID J:103701]

Halberstadt AL; Geyer MA. 2009. Habituation and sensitization of acoustic startle: opposite influences of dopamine D1 and D2-family receptors. Neurobiol Learn Mem 92(2):243-8. [PubMed: 18644244]  [MGI Ref ID J:154428]

Johnson TL; Tulis DA; Keeler BE; Virag JA; Lust RM; Clemens S. 2013. The dopamine D3 receptor knockout mouse mimics aging-related changes in autonomic function and cardiac fibrosis. PLoS One 8(8):e74116. [PubMed: 24023697]  [MGI Ref ID J:205931]

Karasinska JM; George SR; Cheng R; O'Dowd BF. 2005. Deletion of dopamine D1 and D3 receptors differentially affects spontaneous behaviour and cocaine-induced locomotor activity, reward and CREB phosphorylation. Eur J Neurosci 22(7):1741-50. [PubMed: 16197514]  [MGI Ref ID J:102922]

Karasinska JM; George SR; El-Ghundi M; Fletcher PJ; O'Dowd BF. 2000. Modification of dopamine D(1) receptor knockout phenotype in mice lacking both dopamine D(1) and D(3) receptors. Eur J Pharmacol 399(2-3):171-81. [PubMed: 10884517]  [MGI Ref ID J:102600]

Keeler BE; Baran CA; Brewer KL; Clemens S. 2012. Increased excitability of spinal pain reflexes and altered frequency-dependent modulation in the dopamine D3-receptor knockout mouse. Exp Neurol 238(2):273-83. [PubMed: 22995602]  [MGI Ref ID J:193570]

Klinker F; Hasan K; Dowling P; Paulus W; Liebetanz D. 2011. Dopamine D(3) receptor deficiency sensitizes mice to iron deficiency-related deficits in motor learning. Behav Brain Res 220(2):358-61. [PubMed: 21354213]  [MGI Ref ID J:171457]

Le Foll B; Frances H; Diaz J; Schwartz JC; Sokoloff P. 2002. Role of the dopamine D3 receptor in reactivity to cocaine-associated cues in mice. Eur J Neurosci 15(12):2016-26. [PubMed: 12099907]  [MGI Ref ID J:108074]

Maison SF; Liu XP; Eatock RA; Sibley DR; Grandy DK; Liberman MC. 2012. Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes. J Neurosci 32(1):344-355. [PubMed: 22219295]  [MGI Ref ID J:179360]

Marcellino D; Ferre S; Casado V; Cortes A; Le Foll B; Mazzola C; Drago F; Saur O; Stark H; Soriano A; Barnes C; Goldberg SR; Lluis C; Fuxe K; Franco R. 2008. Identification of dopamine D1-D3 receptor heteromers. Indications for a role of synergistic D1-D3 receptor interactions in the striatum. J Biol Chem 283(38):26016-25. [PubMed: 18644790]  [MGI Ref ID J:141997]

Mizuo K; Narita M; Miyatake M; Suzuki T. 2004. Enhancement of dopamine-induced signaling responses in the forebrain of mice lacking dopamine D3 receptor. Neurosci Lett 358(1):13-6. [PubMed: 15016423]  [MGI Ref ID J:107534]

Narita M; Mizuo K; Mizoguchi H; Sakata M; Narita M; Tseng LF; Suzuki T. 2003. Molecular evidence for the functional role of dopamine D3 receptor in the morphine-induced rewarding effect and hyperlocomotion. J Neurosci 23(3):1006-12. [PubMed: 12574430]  [MGI Ref ID J:81915]

Narita M; Soma M; Tamaki H; Narita M; Suzuki T. 2002. Intensification of the development of ethanol dependence in mice lacking dopamine D(3) receptor. Neurosci Lett 324(2):129-32. [PubMed: 11988344]  [MGI Ref ID J:107932]

Short JL; Ledent C; Borrelli E; Drago J; Lawrence AJ. 2006. Genetic interdependence of adenosine and dopamine receptors: Evidence from receptor knockout mice. Neuroscience 139(2):661-70. [PubMed: 16476524]  [MGI Ref ID J:107738]

Song R; Zhang HY; Li X; Bi GH; Gardner EL; Xi ZX. 2012. Increased vulnerability to cocaine in mice lacking dopamine D3 receptors. Proc Natl Acad Sci U S A 109(43):17675-80. [PubMed: 23045656]  [MGI Ref ID J:190377]

Steiner H; Fuchs S; Accili D. 1997. D3 dopamine receptor-deficient mouse: evidence for reduced anxiety. Physiol Behav 63(1):137-41. [PubMed: 9402626]  [MGI Ref ID J:44685]

Vallone D; Pignatelli M; Grammatikopoulos G; Ruocco L; Bozzi Y; Westphal H; Borrelli E; Sadile AG. 2002. Activity, non-selective attention and emotionality in dopamine D2/D3 receptor knock-out mice. Behav Brain Res 130(1-2):141-8. [PubMed: 11864730]  [MGI Ref ID J:96229]

Wong JY; Clifford JJ; Massalas JS; Finkelstein DI; Horne MK; Waddington JL; Drago J. 2003. Neurochemical changes in dopamine D1, D3 and D1/D3 receptor knockout mice. Eur J Pharmacol 472(1-2):39-47. [PubMed: 12860471]  [MGI Ref ID J:103739]

Wong JY; Clifford JJ; Massalas JS; Kinsella A; Waddington JL; Drago J. 2003. Essential conservation of D1 mutant phenotype at the level of individual topographies of behaviour in mice lacking both D1 and D3 dopamine receptors. Psychopharmacology (Berl) 167(2):167-73. [PubMed: 12652349]  [MGI Ref ID J:103886]

Zeng C; Liu Y; Wang Z; He D; Huang L; Yu P; Zheng S; Jones JE; Asico LD; Hopfer U; Eisner GM; Felder RA; Jose PA. 2006. Activation of D3 dopamine receptor decreases angiotensin II type 1 receptor expression in rat renal proximal tubule cells. Circ Res 99(5):494-500. [PubMed: 16902178]  [MGI Ref ID J:125070]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   30-APR-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Drd3tm1Dac  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Drd3tm1Dac x Homozygous for Drd3tm1Dac  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Drd3tm1Dac  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Drd3tm1Dac x Homozygous for Drd3tm1Dac  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
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Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use


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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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