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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+3
Generation DefinitionsDonating Investigator Stephen Jones, Univ of Massachusetts Medical School Description
Mice homozygous for a null mutation in Mdm2 die early in gestation, but are rescued in the absence of Trp53. Mdm2/Trp53-double null mice share the same phenotype as Trp53 mice.
Control Information
| Control | ||
|---|---|---|
| See control note: | Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls. The B6129SF2 mice only provide an approximate match to this mixed B6,129 background. | |
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mdm2tm1Bay/Mdm2+
B6.129S7-Mdm2tm1Bay
- mortality/aging
- increased mortality induced by gamma-irradiation
- 100% of mice die by day 14 after 10 Gy irradiation (MGI Ref ID J:81065)
Mdm2tm1Bay/Mdm2tm1Bay
involves: 129S7/SvEvBrdThis mouse can be used to support research in many areas including:Mdm2tm1Bay related
Apoptosis Research
Endogenous Regulators
Cancer Research
Genes Regulating Growth and Proliferation
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Mdm2tm1Bay | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Baylor College of Medicine | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Mdm2-; Mdm2delta7-12; mdm2ml; | ||
| Mutation Made By | Stephen Jones, Univ of Massachusetts Medical School | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt | ||
| ES Cell Line Name | AB2.1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt | ||
| Gene Symbol and Name | Mdm2, transformed mouse 3T3 cell double minute 2 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | 1700007J15Rik; AA415488; ACTFS; HDMX; Mdm-2; RIKEN cDNA 1700007J15 gene; expressed sequence AA415488; hdm2; | ||
| Molecular Note | An hprt reporter replaced a 7.1 kb fragment of the gene encompassing all of exons 7 through 11 and a portion of exon 12. The deleted region encoded all of the putative transcription function motifs of the protein. [MGI Ref ID J:29811] [MGI Ref ID J:81065] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Human HPRT, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Jones SN; Roe AE; Donehower LA; Bradley A. 1995. Rescue of embryonic lethality in Mdm2-deficient mice by absence of p53. Nature 378(6553):206-8. [PubMed: 7477327] [MGI Ref ID J:29811]
Additional References
Donehower LA; Harvey M; Slagle BL; McArthur MJ; Montgomery CA Jr; Butel JS; Bradley A. 1992. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. Nature 356(6366):215-21. [PubMed: 1552940] [MGI Ref ID J:1999]
Mdm2tm1Bay relatedBianchetta MJ; Lam TT; Jones SN; Morabito MA. 2011. Cyclin-dependent kinase 5 regulates PSD-95 ubiquitination in neurons. J Neurosci 31(33):12029-35. [PubMed: 21849563] [MGI Ref ID J:176050]
Jones SN; Hancock AR; Vogel H; Donehower LA; Bradley A. 1998. Overexpression of Mdm2 in mice reveals a p53-independent role for Mdm2 in tumorigenesis. Proc Natl Acad Sci U S A 95(26):15608-12. [PubMed: 9861017] [MGI Ref ID J:51661]
Jones SN; Sands AT; Hancock AR; Vogel H; Donehower LA; Linke SP; Wahl GM; Bradley A. 1996. The tumorigenic potential and cell growth characteristics of p53-deficient cells are equivalent in the presence or absence of Mdm2. Proc Natl Acad Sci U S A 93(24):14106-11. [PubMed: 8943068] [MGI Ref ID J:37047]
Mendrysa SM; McElwee MK; Michalowski J; O'Leary KA; Young KM; Perry ME. 2003. mdm2 Is Critical for Inhibition of p53 during Lymphopoiesis and the Response to Ionizing Irradiation. Mol Cell Biol 23(2):462-72. [PubMed: 12509446] [MGI Ref ID J:81065]
Mendrysa SM; O'leary KA; McElwee MK; Michalowski J; Eisenman RN; Powell DA; Perry ME. 2006. Tumor suppression and normal aging in mice with constitutively high p53 activity. Genes Dev 20(1):16-21. [PubMed: 16391230] [MGI Ref ID J:104174]
O'Leary KA; Mendrysa SM; Vaccaro A; Perry ME. 2004. Mdm2 regulates p53 independently of p19(ARF) in homeostatic tissues. Mol Cell Biol 24(1):186-91. [PubMed: 14673154] [MGI Ref ID J:87594]
Sluss HK; Armata H; Gallant J; Jones SN. 2004. Phosphorylation of serine 18 regulates distinct p53 functions in mice. Mol Cell Biol 24(3):976-84. [PubMed: 14729946] [MGI Ref ID J:87687]
Steinman HA; Jones SN. 2002. Generation of an Mdm2 conditional allele in mice. Genesis 32(2):142-4. [PubMed: 11857802] [MGI Ref ID J:75119]
Terzian T; Dumble M; Arbab F; Thaller C; Donehower LA; Lozano G; Justice MJ; Roop DR; Box NF. 2011. Rpl27a mutation in the sooty foot ataxia mouse phenocopies high p53 mouse models. J Pathol 224(4):540-52. [PubMed: 21674502] [MGI Ref ID J:174216]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| See control note: | Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls. The B6129SF2 mice only provide an approximate match to this mixed B6,129 background. | |
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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