Strain Name:

B6;129S7-Mdm2tm1Bay/J

Stock Number:

002968

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Stephen N. Jones,   Univ of Massachusetts Medical School

Description
Mice homozygous for a null mutation in Mdm2 die early in gestation, but are rescued in the absence of Trp53. Mdm2/Trp53-double null mice share the same phenotype as Trp53 mice.

Control Information

  Control
   See control note: Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls. The B6129SF2 mice only provide an approximate match to this mixed B6,129 background.
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Accelerated Tumor Formation, Susceptibility To; ACTFS   (MDM2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mdm2tm1Bay/Mdm2+

        B6.129S7-Mdm2tm1Bay
  • mortality/aging
  • increased mortality induced by gamma-irradiation
    • 100% of mice die by day 14 after 10 Gy irradiation   (MGI Ref ID J:81065)
  • homeostasis/metabolism phenotype
  • increased mortality induced by gamma-irradiation
    • 100% of mice die by day 14 after 10 Gy irradiation   (MGI Ref ID J:81065)

Mdm2tm1Bay/Mdm2tm1Bay

        involves: 129S7/SvEvBrd
  • mortality/aging
  • complete embryonic lethality between implantation and somite formation
    • death between E4.5 and E7.5   (MGI Ref ID J:29811)
  • embryogenesis phenotype
  • abnormal embryonic tissue morphology
    • lacked normal embryonic architecture   (MGI Ref ID J:29811)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Mdm2tm1Bay related

Apoptosis Research
Endogenous Regulators

Cancer Research
Genes Regulating Growth and Proliferation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mdm2tm1Bay
Allele Name targeted mutation 1, Baylor College of Medicine
Allele Type Targeted (Null/Knockout)
Common Name(s) Mdm2-; Mdm2delta7-12; mdm2ml;
Mutation Made ByDr. Stephen Jones,   Univ of Massachusetts Medical School
Strain of Origin129S7/SvEvBrd-Hprt
ES Cell Line NameAB2.1
ES Cell Line Strain129S7/SvEvBrd-Hprt
Gene Symbol and Name Mdm2, transformed mouse 3T3 cell double minute 2
Chromosome 10
Gene Common Name(s) 1700007J15Rik; AA415488; ACTFS; HDMX; Mdm-2; RIKEN cDNA 1700007J15 gene; expressed sequence AA415488; hdm2;
Molecular Note An hprt reporter replaced a 7.1 kb fragment of the gene encompassing all of exons 7 through 11 and a portion of exon 12. The deleted region encoded all of the putative transcription function motifs of the protein. [MGI Ref ID J:29811] [MGI Ref ID J:81065]

Genotyping

Genotyping Information

Genotyping Protocols

Human HPRT, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jones SN; Roe AE; Donehower LA; Bradley A. 1995. Rescue of embryonic lethality in Mdm2-deficient mice by absence of p53. Nature 378(6553):206-8. [PubMed: 7477327]  [MGI Ref ID J:29811]

Additional References

Donehower LA; Harvey M; Slagle BL; McArthur MJ; Montgomery CA Jr; Butel JS; Bradley A. 1992. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. Nature 356(6366):215-21. [PubMed: 1552940]  [MGI Ref ID J:1999]

Mdm2tm1Bay related

Bianchetta MJ; Lam TT; Jones SN; Morabito MA. 2011. Cyclin-dependent kinase 5 regulates PSD-95 ubiquitination in neurons. J Neurosci 31(33):12029-35. [PubMed: 21849563]  [MGI Ref ID J:176050]

Jones SN; Hancock AR; Vogel H; Donehower LA; Bradley A. 1998. Overexpression of Mdm2 in mice reveals a p53-independent role for Mdm2 in tumorigenesis. Proc Natl Acad Sci U S A 95(26):15608-12. [PubMed: 9861017]  [MGI Ref ID J:51661]

Jones SN; Sands AT; Hancock AR; Vogel H; Donehower LA; Linke SP; Wahl GM; Bradley A. 1996. The tumorigenic potential and cell growth characteristics of p53-deficient cells are equivalent in the presence or absence of Mdm2. Proc Natl Acad Sci U S A 93(24):14106-11. [PubMed: 8943068]  [MGI Ref ID J:37047]

Lee MK; Tong WM; Wang ZQ; Sabapathy K. 2011. Serine 312 phosphorylation is dispensable for wild-type p53 functions in vivo. Cell Death Differ 18(2):214-21. [PubMed: 20671749]  [MGI Ref ID J:186335]

Mendrysa SM; McElwee MK; Michalowski J; O'Leary KA; Young KM; Perry ME. 2003. mdm2 Is Critical for Inhibition of p53 during Lymphopoiesis and the Response to Ionizing Irradiation. Mol Cell Biol 23(2):462-72. [PubMed: 12509446]  [MGI Ref ID J:81065]

Mendrysa SM; O'leary KA; McElwee MK; Michalowski J; Eisenman RN; Powell DA; Perry ME. 2006. Tumor suppression and normal aging in mice with constitutively high p53 activity. Genes Dev 20(1):16-21. [PubMed: 16391230]  [MGI Ref ID J:104174]

O'Leary KA; Mendrysa SM; Vaccaro A; Perry ME. 2004. Mdm2 regulates p53 independently of p19(ARF) in homeostatic tissues. Mol Cell Biol 24(1):186-91. [PubMed: 14673154]  [MGI Ref ID J:87594]

Sluss HK; Armata H; Gallant J; Jones SN. 2004. Phosphorylation of serine 18 regulates distinct p53 functions in mice. Mol Cell Biol 24(3):976-84. [PubMed: 14729946]  [MGI Ref ID J:87687]

Steinman HA; Jones SN. 2002. Generation of an Mdm2 conditional allele in mice. Genesis 32(2):142-4. [PubMed: 11857802]  [MGI Ref ID J:75119]

Terzian T; Dumble M; Arbab F; Thaller C; Donehower LA; Lozano G; Justice MJ; Roop DR; Box NF. 2011. Rpl27a mutation in the sooty foot ataxia mouse phenocopies high p53 mouse models. J Pathol 224(4):540-52. [PubMed: 21674502]  [MGI Ref ID J:174216]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   See control note: Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls. The B6129SF2 mice only provide an approximate match to this mixed B6,129 background.
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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