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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg(D2)-Tg(xstpx-lacZ)32And/J (Changed: 15-DEC-04 ) B6.Cg(D2)-TgN(xstpxLacZ)32And (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation F?+2
Generation DefinitionsDonating Investigator David J Anderson, California Institute of Technology, HHMI Description
This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter.Development
The cassette is "a loxP-STOP-of-translation-loxP-lacZ" combination. This is driven by a beta-actin promoter. The lacZ is expressed only in cells where the STOP element has been removed. This strain was generated on a (C57BL/6J x DBA/2)F1 genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (256 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
lacZ expression in neural tissue
Cre-lox System
loxP-flanked Sequences
Research Tools
lacZ Expression
Cardiovascular Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(xstpx-lacZ)32And | ||
|---|---|---|---|
| Allele Name | transgene insertion 32, David J Anderson | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | N-lacZ; cAct-XstopXlacZ; cBeta-STOP-lacZ; | ||
| Site of Expression | when crossed with a Cre recombinase expressing strain, lacZ will express in cells where cre is expressed; limited to neural tissue, skeletal muscle, and heart | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Gene Symbol and Name | Tg(xstpx-lacZ)32And, transgene insertion 32, David J Anderson | ||
| Chromosome | UN | ||
| Gene Common Name(s) | N-lacZ; | ||
| General Note |
Line 36 was also produced. The chicken beta actin promoter drives lacZ expression in neural tissue, skeletal muscle tissue, and heart. In conjunction with a cre transgene, transgenic mice express lacZ in cells where Cre is expressed. | ||
| Molecular Note | The transgene is loxP-STOP-of-translation-loxP-lacZ driven by the chicken beta-actin promoter. [MGI Ref ID J:67904] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ QPCR, QPCR
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Zinyk DL; Mercer EH; Harris E; Anderson DJ; Joyner AL. 1998. Fate mapping of the mouse midbrain-hindbrain constriction using a site-specific recombination system. Curr Biol 8(11):665-8. [PubMed: 9635195] [MGI Ref ID J:67904]
Additional References
Tg(xstpx-lacZ)32And relatedAnthony TE; Klein C; Fishell G; Heintz N. 2004. Radial glia serve as neuronal progenitors in all regions of the central nervous system. Neuron 41(6):881-90. [PubMed: 15046721] [MGI Ref ID J:89775]
Barlow C; Schroeder M; Lekstrom-Himes J; Kylefjord H; Deng CX; Wynshaw-Boris A; Spiegelman BM; Xanthopoulos KG. 1997. Targeted expression of Cre recombinase to adipose tissue of transgenic mice directs adipose-specific excision of loxP-flanked gene segments. Nucleic Acids Res 25(12):2543-5. [PubMed: 9171115] [MGI Ref ID J:67907]
Bergqvist I; Eriksson B; Eriksson M; Holmberg D. 1998. Transgenic Cre recombinase expression in germ cells and early embryogenesis directs homogeneous and ubiquitous deletion of loxP-flanked gene segments. FEBS Lett 438(1-2):76-80. [PubMed: 9821962] [MGI Ref ID J:88598]
Bielle F; Griveau A; Narboux-Neme N; Vigneau S; Sigrist M; Arber S; Wassef M; Pierani A. 2005. Multiple origins of Cajal-Retzius cells at the borders of the developing pallium. Nat Neurosci 8(8):1002-12. [PubMed: 16041369] [MGI Ref ID J:101434]
Cui Z; Lindl KA; Mei B; Zhang S; Tsien JZ. 2005. Requirement of NMDA receptor reactivation for consolidation and storage of nondeclarative taste memory revealed by inducible NR1 knockout. Eur J Neurosci 22(3):755-63. [PubMed: 16101757] [MGI Ref ID J:100903]
Eriksson B; Bergqvist I; Eriksson M; Holmberg D. 2000. Functional expression of Cre recombinase in sub-regions of mouse CNS and retina. FEBS Lett 479(3):106-10. [PubMed: 10981716] [MGI Ref ID J:82026]
Feng L; Xie ZH; Ding Q; Xie X; Libby RT; Gan L. 2010. MATH5 controls the acquisition of multiple retinal cell fates. Mol Brain 3:36. [PubMed: 21087508] [MGI Ref ID J:168595]
Guo H; Mao C; Jin XL; Wang H; Tu YT; Avasthi PP; Li Y. 2000. Cre-mediated cerebellum- and hippocampus-restricted gene mutation in mouse brain. Biochem Biophys Res Commun 269(1):149-54. [PubMed: 10694492] [MGI Ref ID J:114231]
Hirasawa M; Cho A; Sreenath T; Sauer B; Julien JP; Kulkarni AB. 2001. Neuron-specific expression of Cre recombinase during the late phase of brain development. Neurosci Res 40(2):125-32. [PubMed: 11377750] [MGI Ref ID J:82025]
Huang ZJ; Yu W; Lovett C; Tonegawa S. 2002. Cre/loxP recombination-activated neuronal markers in mouse neocortex and hippocampus. Genesis 32(3):209-17. [PubMed: 11892010] [MGI Ref ID J:75827]
Kellendonk C; Tronche F; Casanova E; Anlag K; Opherk C; Schutz G. 1999. Inducible site-specific recombination in the brain. J Mol Biol 285(1):175-82. [PubMed: 9878397] [MGI Ref ID J:69323]
Kogata N; Arai Y; Pearson JT; Hashimoto K; Hidaka K; Koyama T; Somekawa S; Nakaoka Y; Ogawa M; Adams RH; Okada M; Mochizuki N. 2006. Cardiac ischemia activates vascular endothelial cadherin promoter in both preexisting vascular cells and bone marrow cells involved in neovascularization. Circ Res 98(7):897-904. [PubMed: 16543497] [MGI Ref ID J:121366]
Korets-Smith E; Lindemann L; Tucker KL; Jiang C; Kabacs N; Belteki G; Haigh J; Gertsenstein M; Nagy A. 2004. Cre recombinase specificity defined by the tau locus. Genesis 40(3):131-8. [PubMed: 15493019] [MGI Ref ID J:94203]
Leshan RL; Bjornholm M; Munzberg H; Myers MG Jr. 2006. Leptin receptor signaling and action in the central nervous system. Obesity (Silver Spring) 14 Suppl 5:208S-212S. [PubMed: 17021368] [MGI Ref ID J:131433]
Lindeberg J; Mattsson R; Ebendal T. 2002. Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene. J Neurosci Res 68(2):248-53. [PubMed: 11948670] [MGI Ref ID J:82535]
Lindeberg J; Usoskin D; Bengtsson H; Gustafsson A; Kylberg A; Soderstrom S; Ebendal T. 2004. Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus. Genesis 40(2):67. [PubMed: 15452869] [MGI Ref ID J:93194]
Peng CY; Yajima H; Burns CE; Zon LI; Sisodia SS; Pfaff SL; Sharma K. 2007. Notch and MAML signaling drives Scl-dependent interneuron diversity in the spinal cord. Neuron 53(6):813-27. [PubMed: 17359917] [MGI Ref ID J:132095]
Shen HM; Peters A; Kao D; Storb U. 2001. The 3' Igkappa enhancer contains RNA polymerase II promoters: implications for endogenous and transgenic kappa gene expression. Int Immunol 13(5):665-74. [PubMed: 11312254] [MGI Ref ID J:69344]
Skidmore JM; Cramer JD; Martin JF; Martin DM. 2008. Cre fate mapping reveals lineage specific defects in neuronal migration with loss of Pitx2 function in the developing mouse hypothalamus and subthalamic nucleus. Mol Cell Neurosci 37(4):696-707. [PubMed: 18206388] [MGI Ref ID J:135239]
Tsien JZ; Chen DF; Gerber D; Tom C; Mercer EH; Anderson DJ; Mayford M; Kandel ER; Tonegawa S. 1996. Subregion- and cell type-restricted gene knockout in mouse brain [see comments] Cell 87(7):1317-26. [PubMed: 8980237] [MGI Ref ID J:66344]
Waite MR; Skidmore JM; Billi AC; Martin JF; Martin DM. 2011. GABAergic and glutamatergic identities of developing midbrain Pitx2 neurons. Dev Dyn 240(2):333-46. [PubMed: 21246650] [MGI Ref ID J:167837]
Zhang J; Zhang L; Jiao H; Zhang Q; Zhang D; Lou D; Katz JL; Xu M. 2006. c-Fos facilitates the acquisition and extinction of cocaine-induced persistent changes. J Neurosci 26(51):13287-96. [PubMed: 17182779] [MGI Ref ID J:116774]
Zhu Y; Romero MI; Ghosh P; Ye Z; Charnay P; Rushing EJ; Marth JD; Parada LF. 2001. Ablation of NF1 function in neurons induces abnormal development of cerebral cortex and reactive gliosis in the brain. Genes Dev 15(7):859-76. [PubMed: 11297510] [MGI Ref ID J:68558]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice were bred as homozygotes. Coat color expected from breeding:Black Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3175.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $4127.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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