Strain Name:

B6.129S4-Nos1tm1Plh/J

Stock Number:

002986

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Mice homozygous for Nos1tm1Plh have enlarged stomachs with hypertrophy of the pyloric sphincter and the circular muscle layer. This phenotype resembles the human disorder infantile pyloric stenosis, in which gastric outlet obstruction is associated with the lack of NADPH-diaphorase neurons in the pylorus.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
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Mating SystemHomozygote x Heterozygote         (Female x Male)   04-APR-13
Mating SystemHeterozygote x Homozygote         (Female x Male)   04-APR-13
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S4 via J1 ES cell line
GenerationN11+N4F3 (11-DEC-13)
Generation Definitions
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Important Note
The mice of this strain have been backcrossed more than 10 backcrosses to C57BL/6J, making this strain more effective for studies of the Nos1 KO than the incipient stock (#002633).

Description
The nitric oxide signaling molecule produced by the neuronal Nos1 gene is widely expressed, affecting diverse systems. The knockout of this gene has been shown to affect such normal functions as cognition and behavior, long-term memory, cortical development, colonic motility, skeletal muscle contractility, ovarian cycle regulation, cardiac/cardiovascular function, and certain immune system responses. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. Mice homozygous for the Nos1tm1Plh targeted mutation are viable and fertile. The first publication describing this knock-out reported that mutants have enlarged stomachs with hypertrophy of the pyloric sphincter and the circular muscle layer (Huang et al., 1993). Male homozygotes of this strain were initially reported to be more aggressive than control mice, but this has been since attributed to heterogeneity of the stock before it was sufficiently backcrossed to the C57BL/6J genetic background. Mice of this congenic strain in the Jackson Laboratory colony show normal behavior in this regard.

Development
The mutation was developed by Dr. Paul Huang, Mass General Hospital, Harvard Medical School. A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Correctly targeted Jl ES cells were injected into C57BL/6 blastocysts and resulting chimeras were backcrossed to C57BL/6 mice for a total of 12 generations before being maintained by inbreeding.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Nos1tm1Plh allele
002633   B6;129S4-Nos1tm1Plh/J
View Strains carrying   Nos1tm1Plh     (1 strain)

Strains carrying other alleles of Nos1
017526   B6.129-Nos1tm1(cre)Mgmj/J
008519   B6.129S4-Nos1tm2Plh/J
014541   B6;129S-Nos1tm1.1(cre/ERT2)Zjh/J
View Strains carrying other alleles of Nos1     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.
Achalasia, Familial Esophageal
Pyloric Stenosis, Infantile Hypertrophic, 1; IHPS1
- No similarity to the expected human disease phenotype was found. One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of these genes, but the phenotype did not resemble the disease.
Pyloric Stenosis, Infantile Hypertrophic, 1; IHPS1
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Nos1tm1Plh/Nos1+

        B6.129S4-Nos1tm1Plh
  • cardiovascular system phenotype
  • abnormal cardiovascular system physiology
    • exercise training fails to augment the phenylephrine induced pulse interval response   (MGI Ref ID J:105579)
    • abnormal heart rate
      • exercise training fails to augment the phenylephrine induced or vagal nerve stimulation bradycardia responses   (MGI Ref ID J:105579)
      • no differences in heart rate responses are detected in the absence of exercise training   (MGI Ref ID J:105579)
  • homeostasis/metabolism phenotype
  • abnormal physiological response to xenobiotic
    • exercise training fails to augment the phenylephrine induced bradycardia and pulse interval responses   (MGI Ref ID J:105579)
    • no differences in phenylephrine induced responses are seen in untrained mice   (MGI Ref ID J:105579)

Nos1tm1Plh/Nos1tm1Plh

        B6.129S4-Nos1tm1Plh
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • unlike mice null for Nos3, ischemia induced retinal neovascularization is not significantly different from controls   (MGI Ref ID J:106207)
    • abnormal blood-brain barrier function
      • kainic acid injection of hippocampi does not cause blood-brain barrier breakdown   (MGI Ref ID J:150557)
    • decreased susceptibility to induced choroidal neovascularization
      • knockouts show a 58% decrease in neovascularization at sites of laser-induced Bruch's membrane rupture compared to heterozygous littermates   (MGI Ref ID J:106207)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • unlike mice on a mixed 129S4/SvJae and C57BL/6J background, congenic male mice do not display significantly increased aggression compared to wild-type controls   (MGI Ref ID J:67776)
    • abnormal maternal behavior
      • lactating females display decreased aggression towards intruder males   (MGI Ref ID J:57543)
      • about a 90% decrease in the amount of time lactating females spend actively attacking or biting an intruder male   (MGI Ref ID J:57543)
      • in contrast to the decrease in maternal aggression, no defect in pup retrieval is detected   (MGI Ref ID J:57543)
    • abnormal seizure response to inducing agent
      • when exposed to hyperbaric oxygen (greater than 99% oxygen) at 5 ATA for 60 min the time to first seizure in freely moving mice is significantly longer compared to similarly treated wild-type mice   (MGI Ref ID J:107964)
    • decreased aggression towards males
      • lactating females display decreased aggression towards intruder males   (MGI Ref ID J:57543)
      • about a 90% decrease in the amount of time lactating females spend actively attacking or biting an intruder male   (MGI Ref ID J:57543)
    • increased aggression towards males
      • increase in the number and duration of attacks and decrease in the latency to attack in a resident intruder assay   (MGI Ref ID J:127032)
      • treatment with a serotonin precursor dramatically reduces aggressive behavior   (MGI Ref ID J:127032)
      • significantly higher concentrations of serotonin receptor agonists are required to reduce aggressive behavior than in wild-type controls   (MGI Ref ID J:127032)
  • other phenotype
  • maternal effect
    • pups of homozygous females weigh more than pups of wild-type females   (MGI Ref ID J:57543)
  • homeostasis/metabolism phenotype
  • abnormal serotonin level
    • total serotonin content is increased in the cerebral cortex, hypothalamus, hippocampus, midbrain and cerebellum   (MGI Ref ID J:127032)
    • as no change is seen on the concentration of the serotonin metabolite, 5-HIAA, the 5-HIAA to serotonin (5-HT) ratio is reduced in the cortex, hypothalamus, midbrain, and cerebellum indicating a decrease in serotonin turnover   (MGI Ref ID J:127032)
  • decreased adiponectin level   (MGI Ref ID J:155090)
  • decreased susceptibility to ischemic brain injury
    • functional deficits following middle cerebral artery obstruction are reduced compared to wild-type controls   (MGI Ref ID J:128835)
    • decreased cerebral infarction size
      • infarction sized induced by middle cerebral artery obstruction is reduced in males, but not in females, compared to wild-type controls   (MGI Ref ID J:128835)
      • however, infarct size is increased compared to mice hemizygous for Tg(SOD1)76Dpr   (MGI Ref ID J:128835)
  • decreased susceptibility to neuronal excitotoxicity
    • kainic acid does not cause the hippocampal neurons to degenerate   (MGI Ref ID J:150557)
  • increased circulating triglyceride level
    • elevated plasma triglycerides   (MGI Ref ID J:155090)
  • vision/eye phenotype
  • decreased susceptibility to induced choroidal neovascularization
    • knockouts show a 58% decrease in neovascularization at sites of laser-induced Bruch's membrane rupture compared to heterozygous littermates   (MGI Ref ID J:106207)
  • nervous system phenotype
  • abnormal nervous system physiology
    • in anesthetized mice the latency to hyperbaric oxygen (greater than 99% oxygen) at 5 ATA induced EEG seizure is increased compared to similarly treated wild-type mice   (MGI Ref ID J:107964)
    • striatal accumulation of 3-nitrotyrosine during hyperbaric oxygen exposure is decreased compared to similarly treated wild-type mice   (MGI Ref ID J:107964)
    • abnormal blood-brain barrier function
      • kainic acid injection of hippocampi does not cause blood-brain barrier breakdown   (MGI Ref ID J:150557)
    • abnormal seizure response to inducing agent
      • when exposed to hyperbaric oxygen (greater than 99% oxygen) at 5 ATA for 60 min the time to first seizure in freely moving mice is significantly longer compared to similarly treated wild-type mice   (MGI Ref ID J:107964)
    • decreased susceptibility to ischemic brain injury
      • functional deficits following middle cerebral artery obstruction are reduced compared to wild-type controls   (MGI Ref ID J:128835)
      • decreased cerebral infarction size
        • infarction sized induced by middle cerebral artery obstruction is reduced in males, but not in females, compared to wild-type controls   (MGI Ref ID J:128835)
        • however, infarct size is increased compared to mice hemizygous for Tg(SOD1)76Dpr   (MGI Ref ID J:128835)
    • decreased susceptibility to neuronal excitotoxicity
      • kainic acid does not cause the hippocampal neurons to degenerate   (MGI Ref ID J:150557)
  • cellular phenotype
  • decreased susceptibility to neuronal excitotoxicity
    • kainic acid does not cause the hippocampal neurons to degenerate   (MGI Ref ID J:150557)

Nos1tm1Plh/Nos1tm1Plh

        B6.129S4-Nos1tm1Plh/J
  • growth/size/body phenotype
  • decreased body weight
    • in males only   (MGI Ref ID J:144588)
  • muscle phenotype
  • decreased skeletal muscle weight
    • decrease in skeletal muscle weight in males   (MGI Ref ID J:144588)
  • impaired skeletal muscle contractility
    • peak twitch force is decreased in the tibialis anterior muscle of male mice   (MGI Ref ID J:144588)
    • decrease in the maximal tetanic force generating capacity of the tibialis anterior muscle in males   (MGI Ref ID J:144588)
    • however, when normalized to muscle size the force generating capacity is not significantly different from wild-type controls   (MGI Ref ID J:144588)
  • muscle fatigue
    • tibialis anterior muscle displays an increase in the susceptibility to contraction induced fatigue in both males and females   (MGI Ref ID J:144588)
  • immune system phenotype
  • decreased inflammatory response
    • fever in response to LPS injection is partially reduced compared to wild-type controls   (MGI Ref ID J:103018)
    • reduction in fever response mainly occurs during the early phase of fever response   (MGI Ref ID J:103018)
    • however, fever in response to turpentine injection is not different from controls   (MGI Ref ID J:103018)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Nos1tm1Plh/Nos1tm1Plh

        involves: 129S4/SvJae * C57BL/6
  • mortality/aging
  • decreased sensitivity to induced morbidity/mortality
    • in anesthetized mice under 12% oxygen all mutant mice survived while 4 of 14 wild-type mice died   (MGI Ref ID J:106328)
  • decreased survivor rate
    • survival at 20 months is reduced compared to Nos3 null mice   (MGI Ref ID J:129064)
  • premature death
    • slight increase in premature death   (MGI Ref ID J:16390)
    • survival at 20 months is reduced compared to Nos3 null mice   (MGI Ref ID J:129064)
    • the relative risk of death by 20 months of age is 2.5 times higher than in Nos3 null mice   (MGI Ref ID J:129064)
  • digestive/alimentary phenotype
  • abnormal stomach wall morphology
    • hypertrophy of the pyloric sphincter and the circular muscle layer   (MGI Ref ID J:16390)
    • with age, the stomachs lose their interior folds and undergo thinning of the walls, however the intestine appears normal   (MGI Ref ID J:16390)
    • stomach smooth muscle circular layer hypertrophy   (MGI Ref ID J:16390)
  • enlarged stomach
    • stomach dilation, however architecture of the stomach layers is preserved   (MGI Ref ID J:16390)
  • pyloric sphincter hypertrophy   (MGI Ref ID J:16390)
  • pyloric stenosis   (MGI Ref ID J:16390)
  • reproductive system phenotype
  • abnormal ovulation
    • ovulatory efficiency (number oocytes recovered divided by the number of ovarian rupture sites) is reduced in homozygotes   (MGI Ref ID J:89701)
    • significantly fewer oocytes are recovered following gonadotropin-stimulated ovulation in homozygous mutant females compared to wild-type females   (MGI Ref ID J:89701)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • homozygous mice do not exhibit defects in brain structure or neuron degeneration   (MGI Ref ID J:16390)
    • abnormal brain morphology
      • electron microscopy analysis indicates a lower density of mitochondria in the cortex of the brain   (MGI Ref ID J:104558)
      • however, the ratio of mitochondrial to nuclear DNA is similar to controls   (MGI Ref ID J:104558)
    • abnormal dendrite morphology
      • motor neurons display a significant decrease in the number of dendritic branches   (MGI Ref ID J:111340)
      • decrease in the number of branches is first detected at about 100 um from the cell body with a peak in the decrease between about 160 - 260 um   (MGI Ref ID J:111340)
      • difference in branch number is limited to third and fourth order branches   (MGI Ref ID J:111340)
      • however, the number of primary dendrites and the longest dendritic path from a cell are not different from wild-type controls   (MGI Ref ID J:111340)
    • abnormal motor neuron morphology
      • motor neurons display a significant decrease in the number of dendritic branches   (MGI Ref ID J:111340)
      • however, the number of primary dendrites, the longest dendritic path from a cell, and cell body size are not different from wild-type controls   (MGI Ref ID J:111340)
    • abnormal nervous system physiology
      • delay in Wallerian degeneration following transection of the sciatic nerve in the right hindlimb   (MGI Ref ID J:104916)
      • abnormal peripheral nervous system regeneration
        • following transection of the sciatic nerve in the right hindlimb time to recovery of motor function is delayed   (MGI Ref ID J:104916)
        • following transection of the sciatic nerve in the right hindlimb uncontrolled sprouting is increased   (MGI Ref ID J:104916)
        • however, myelination following transection is not significantly different from controls   (MGI Ref ID J:104916)
  • muscle phenotype
  • abnormal muscle physiology
    • muscle atrophy induced by transection of the sciatic nerve in the right hindlimb is reduced   (MGI Ref ID J:104916)
    • abnormal cardiac muscle relaxation
      • time to 50% relaxation is increased   (MGI Ref ID J:103346)
    • decreased cardiac muscle contractility
      • attenuation of the beta-adrenergic inotropic responses indicating a decrease in myocardial contractile reserve   (MGI Ref ID J:75645)
    • increased ventricle muscle contractility
      • isolated ventricular myocytes display greater overall shortening at all stimulation frequencies between 0.2 and 10 Hz   (MGI Ref ID J:103346)
      • shortening in response to beta-adrenergic stimulation is greatly enhanced   (MGI Ref ID J:103346)
  • pyloric sphincter hypertrophy   (MGI Ref ID J:16390)
  • stomach smooth muscle circular layer hypertrophy   (MGI Ref ID J:16390)
  • behavior/neurological phenotype
  • abnormal mating frequency
    • when placed with females in estrus males display excessive and inappropriate mounting   (MGI Ref ID J:29970)
    • the number of mounts fails to decrease significantly 1 h after the introduction of an anestrus female to the cage   (MGI Ref ID J:29970)
    • by 7 to 8 h after introduction of a female, the number of mounts made by males is 2 to 3 times greater compared to wild-type males   (MGI Ref ID J:29970)
  • increased aggression towards females
    • when placed with females in estrus males display excessive and inappropriate mounting   (MGI Ref ID J:29970)
  • increased aggression towards males
    • in a resident intruder assay males display more aggressive encounters and initiate more of the aggressive encounters compared to wild-type mice   (MGI Ref ID J:29970)
    • for males latency to first attack is reduced to about 1/5 that of wild-type mice   (MGI Ref ID J:29970)
    • male mice display submissive postures about 1/10 as frequently as wild-type controls   (MGI Ref ID J:29970)
    • for males the duration of aggressive encounters is increased   (MGI Ref ID J:29970)
    • no increase in aggression is seen in females   (MGI Ref ID J:29970)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • despite the increase in aggressive and sexual behaviors, no significant difference is detected in blood testosterone levels compared to wild-type males   (MGI Ref ID J:29970)
    • abnormal carbon dioxide production
      • in awake mice carbon dioxide production is significantly decreased during 12% oxygen compared to 100% oxygen in mutant but not in wild-type mice   (MGI Ref ID J:106328)
    • abnormal oxygen consumption
      • in awake mice the magnitude of the decrease in oxygen consumption under 21% or 12% oxygen is greater than that in wild-type controls   (MGI Ref ID J:106328)
      • increased oxygen consumption   (MGI Ref ID J:106328)
    • increased physiological sensitivity to xenobiotic
      • sodium cyanide induced respiratory stimulation is increased in mutants compared to wild-type mice   (MGI Ref ID J:106328)
  • respiratory system phenotype
  • abnormal breathing pattern
    • anesthetized mice under 21% or 12% oxygen show period increases in respiratory rate not seen in wild-type mice   (MGI Ref ID J:106328)
    • abnormal pulmonary respiratory rate
      • in awake mice, the magnitude of the decrease in respiratory rate following brief exposure to 100% oxygen was greater than that in wild-type mice   (MGI Ref ID J:106328)
      • increased pulmonary respiratory rate
        • in awake and anesthetized mice the magnitude of the increases in respiratory rate at 21% and 12% oxygen are greater than in wild-type controls   (MGI Ref ID J:106328)
        • anesthetized mice do not display respiratory depression under 12% oxygen, unlike wild-type controls   (MGI Ref ID J:106328)
        • in anesthetized mutant mice the increase in ventilation under 21% oxygen results from an increase in both respiratory rate and tidal phrenic activity, unlike in wild-type mice where only tidal phrenic activity is increased   (MGI Ref ID J:106328)
        • sodium cyanide induced respiratory stimulation is increased in mutants compared to wild-type mice   (MGI Ref ID J:106328)
  • increased pulmonary ventilation
    • in awake mice the significant increases are seen in minute ventilation at both 21% and 12% oxygen unlike in wild-type controls where increases are seen only at 12% oxygen   (MGI Ref ID J:106328)
    • in awake and anesthetized mice the magnitude of the increase in minute ventilation is greater at both 21% and 12% oxygen compared to wild-type controls   (MGI Ref ID J:106328)
  • increased tidal volume
    • in awake mice the magnitude of the increases in tidal volume at 21% and 12% oxygen are greater than in wild-type controls   (MGI Ref ID J:106328)
  • cardiovascular system phenotype
  • abnormal cardiac muscle relaxation
    • time to 50% relaxation is increased   (MGI Ref ID J:103346)
  • decreased cardiac muscle contractility
    • attenuation of the beta-adrenergic inotropic responses indicating a decrease in myocardial contractile reserve   (MGI Ref ID J:75645)
  • heart left ventricle hypertrophy
    • age related   (MGI Ref ID J:75645)
  • increased ventricle muscle contractility
    • isolated ventricular myocytes display greater overall shortening at all stimulation frequencies between 0.2 and 10 Hz   (MGI Ref ID J:103346)
    • shortening in response to beta-adrenergic stimulation is greatly enhanced   (MGI Ref ID J:103346)
  • cellular phenotype
  • abnormal mitochondrial physiology
    • despite similar ratios of mitochondrial to nuclear DNA the amount of citrate synthase is significantly increased in homogenates from the heart, kidney and liver   (MGI Ref ID J:104558)
  • liver/biliary system phenotype
  • abnormal liver morphology
    • lipid content of the liver is increased   (MGI Ref ID J:104558)
    • unlike in wild-type livers, glycogen content is increased in zone 3 relative to zone 1 of the liver   (MGI Ref ID J:133437)
    • abnormal hepatocyte morphology
      • fat droplets are absent from the cytosol of hepatocytes   (MGI Ref ID J:133437)
  • renal/urinary system phenotype
  • abnormal renal tubule morphology
    • electron microscopy analysis indicates a lower density of mitochondria in tubule cells of the kidney   (MGI Ref ID J:104558)
    • however, the ratio of mitochondrial to nuclear DNA is similar to controls   (MGI Ref ID J:104558)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • no difference in body weight is detected at 1 year of age   (MGI Ref ID J:133437)

Nos1tm1Plh/Nos1tm1Plh

        involves: 129S4/SvJae
  • mortality/aging
  • decreased survivor rate
    • about 80% survival rate at 10 months of age   (MGI Ref ID J:100308)
  • premature death
    • about 20% die before 10 months of age   (MGI Ref ID J:100308)
  • homeostasis/metabolism phenotype
  • abnormal vascular wound healing
    • following carotid artery ligation the extent of neointimal formation is increased and luminal narrowing is worsened compared to wild-type controls   (MGI Ref ID J:120009)
  • altered response of heart to induced stress
    • myocardial infarct size following 30 min of global ischemia is increased compared to controls   (MGI Ref ID J:60525)
  • decreased susceptibility to injury
    • 24 h after aspiration bulbectomy the number of apoptotic cells in the piriform cortex is reduced compared to similarly treated wild-type mice   (MGI Ref ID J:93439)
    • decreased cerebral infarction size
      • following occlusion of the middle cerebral artery infarct size is decreased   (MGI Ref ID J:127062)
  • decreased susceptibility to neuronal excitotoxicity
    • the size of striatal lesions induced by malonate are significantly reduced compared to similarly treated controls   (MGI Ref ID J:111644)
  • insulin resistance
    • insulin resistance in peripheral tissues   (MGI Ref ID J:62229)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • despite the ability of NOS inhibitors to decrease long term potentiation, no significant decrease in long term potentiation is detected at 1 h after tetanus   (MGI Ref ID J:37956)
    • unlike cerebral blood flow, the intensity of neural activation induced by perioral stimulation is not significantly different from controls   (MGI Ref ID J:84255)
    • abnormal nervous system physiology
      • 24 h after aspiration bulbectomy the number of apoptotic cells in the piriform cortex is reduced compared to similarly treated wild-type mice   (MGI Ref ID J:93439)
      • abnormal long term potentiation
        • following a theta burst protocol at a weak intensity enhancement of EPSPs during the first 3 minutes after stimulation is significantly small compared to controls   (MGI Ref ID J:19282)
        • however, no significant differences are detected when a stronger intensity stimulation is used   (MGI Ref ID J:19282)
      • absent long term depression
        • stimulation of parallel fibers with a 50 msec postsynaptic depolarizations fails to induce long term depression in Purkinje cells   (MGI Ref ID J:42548)
        • addition of uncaged NO or photoreleased cGMP to the stimulation protocol also fails to induce long term depression in Purkinje cells, unlike in wild-type mice   (MGI Ref ID J:42548)
      • decreased cerebral infarction size
        • following occlusion of the middle cerebral artery infarct size is decreased   (MGI Ref ID J:127062)
      • decreased susceptibility to neuronal excitotoxicity
        • the size of striatal lesions induced by malonate are significantly reduced compared to similarly treated controls   (MGI Ref ID J:111644)
      • decreased synaptic glutamate release
        • glutamate release after NMDA stimulation is significantly attenuated in the cerebral cortex, partially reduced in the striatum but unaffected in the hippocampus   (MGI Ref ID J:119052)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • unlike in earlier reports males show no signs of increased aggression   (MGI Ref ID J:42548)
    • no defects in circadian rhythms are detected in a wheel running assay either under entrained or free running conditions   (MGI Ref ID J:56005)
    • impaired balance
      • mice show no improvement in their latency to fall off a pole or plank in the dark versus the light phase, unlike in wild-type controls resulting in deficits in balance compared to wild-type mice during the dark phase   (MGI Ref ID J:56005)
  • digestive/alimentary phenotype
  • abnormal gastroesophageal sphincter physiology
    • lower esophageal sphincters fail to display electrical field stimulation (60 V, 5 Hz) induced relaxation or rebound contraction   (MGI Ref ID J:56948)
    • the mean resting lower esophageal sphincter pressure is significantly higher   (MGI Ref ID J:70182)
    • swallowing induced lower esophageal sphincter relaxation is significantly attenuated   (MGI Ref ID J:70182)
    • mice show a range of responses to pharyngeal stimulation including no relaxation, partial relaxation , and sporadic near complete relaxation of the lower esophageal sphincter   (MGI Ref ID J:70182)
    • efferent vagal stimulation induced lower esophageal sphincter relaxation is significantly attenuated   (MGI Ref ID J:70182)
  • immune system phenotype
  • abnormal leukocyte adhesion
    • dramatic increase in baseline leukocyte adherence   (MGI Ref ID J:55936)
    • treatment with an anti-P-selectin antibody significantly reduces the adhesion response in mutant but not in wild-type mice   (MGI Ref ID J:55936)
  • abnormal leukocyte tethering or rolling
    • increase in baseline rolling   (MGI Ref ID J:55936)
    • treatment with an anti-P-selectin antibody significantly reduces the rolling response in mutant but not in wild-type mice   (MGI Ref ID J:55936)
    • treatment with a low dose of thrombin significantly increases rolling in mutant but not in wild-type mice   (MGI Ref ID J:55936)
  • abnormal neutrophil physiology
    • following thioglycollate injection neutrophil accumulation in the peritoneum is significantly enhanced compared to wild-type mice   (MGI Ref ID J:55936)
  • muscle phenotype
  • abnormal muscle physiology
    • the resting membrane potential of jejunal smooth muscle cells is depolarized about 5 mV compared to wild type cells   (MGI Ref ID J:89581)
    • hyperpolarization and induction of an inhibitory junction potential in response to electrical field stimulation under nonadrenergic noncholinergic conditions in jejunal muscle strips are markedly reduced   (MGI Ref ID J:89581)
    • abnormal gastroesophageal sphincter physiology
      • lower esophageal sphincters fail to display electrical field stimulation (60 V, 5 Hz) induced relaxation or rebound contraction   (MGI Ref ID J:56948)
      • the mean resting lower esophageal sphincter pressure is significantly higher   (MGI Ref ID J:70182)
      • swallowing induced lower esophageal sphincter relaxation is significantly attenuated   (MGI Ref ID J:70182)
      • mice show a range of responses to pharyngeal stimulation including no relaxation, partial relaxation , and sporadic near complete relaxation of the lower esophageal sphincter   (MGI Ref ID J:70182)
      • efferent vagal stimulation induced lower esophageal sphincter relaxation is significantly attenuated   (MGI Ref ID J:70182)
    • abnormal muscle relaxation
      • lower esophageal sphincters fail to display electrical field stimulation (60 V, 5 Hz) induced relaxation or rebound contraction   (MGI Ref ID J:56948)
      • the decrease in muscle contractions in jejunal muscle strips in response to electrical field stimulation is markedly reduced   (MGI Ref ID J:89581)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • unlike in mice null for Nos3 no significant abnormalities in hypoxic pulmonary vasoconstriction, vasodilation in response to bradykinin, or right ventricle systolic pressure are detected   (MGI Ref ID J:57624)
    • abnormal blood circulation
      • the increase in cerebral blood flow induced by glutamate, perioral stimulation, or harmaline is significantly attenuated   (MGI Ref ID J:84255)
      • decreased coronary flow rate
        • in isolated perfused hearts   (MGI Ref ID J:60525)
        • however, coronary flow per beat is not significantly different from controls   (MGI Ref ID J:60525)
    • abnormal vascular wound healing
      • following carotid artery ligation the extent of neointimal formation is increased and luminal narrowing is worsened compared to wild-type controls   (MGI Ref ID J:120009)
    • altered response of heart to induced stress
      • myocardial infarct size following 30 min of global ischemia is increased compared to controls   (MGI Ref ID J:60525)
    • decreased heart weight   (MGI Ref ID J:60525)
  • vision/eye phenotype
  • *normal* vision/eye phenotype
    • no defects are detected in visual acuity   (MGI Ref ID J:56005)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • unlike in Nos3 null mice, the increase in maximal penile intracavernous pressure induced by papaverine is not significantly different from wild-type controls   (MGI Ref ID J:89704)
  • growth/size/body phenotype
  • decreased body weight   (MGI Ref ID J:60525)
  • cellular phenotype
  • abnormal leukocyte adhesion
    • dramatic increase in baseline leukocyte adherence   (MGI Ref ID J:55936)
    • treatment with an anti-P-selectin antibody significantly reduces the adhesion response in mutant but not in wild-type mice   (MGI Ref ID J:55936)
  • abnormal leukocyte tethering or rolling
    • increase in baseline rolling   (MGI Ref ID J:55936)
    • treatment with an anti-P-selectin antibody significantly reduces the rolling response in mutant but not in wild-type mice   (MGI Ref ID J:55936)
    • treatment with a low dose of thrombin significantly increases rolling in mutant but not in wild-type mice   (MGI Ref ID J:55936)
  • decreased susceptibility to neuronal excitotoxicity
    • the size of striatal lesions induced by malonate are significantly reduced compared to similarly treated controls   (MGI Ref ID J:111644)
  • hematopoietic system phenotype
  • abnormal leukocyte adhesion
    • dramatic increase in baseline leukocyte adherence   (MGI Ref ID J:55936)
    • treatment with an anti-P-selectin antibody significantly reduces the adhesion response in mutant but not in wild-type mice   (MGI Ref ID J:55936)
  • abnormal leukocyte tethering or rolling
    • increase in baseline rolling   (MGI Ref ID J:55936)
    • treatment with an anti-P-selectin antibody significantly reduces the rolling response in mutant but not in wild-type mice   (MGI Ref ID J:55936)
    • treatment with a low dose of thrombin significantly increases rolling in mutant but not in wild-type mice   (MGI Ref ID J:55936)
  • abnormal neutrophil physiology
    • following thioglycollate injection neutrophil accumulation in the peritoneum is significantly enhanced compared to wild-type mice   (MGI Ref ID J:55936)

Nos1tm1Plh/Nos1tm1Plh

        involves: 129S4/SvJae * C57BL/6J
  • renal/urinary system phenotype
  • abnormal renal reabsorbtion
    • the rate of bicarbonate absorption is reduced in the proximal tubules   (MGI Ref ID J:64896)
    • abnormal renal water reabsorbtion
      • the rate of net fluid absorption is reduced in the proximal tubules   (MGI Ref ID J:64896)
  • increased urine bicarbonate level
    • increase in urine bicarbonate concentration   (MGI Ref ID J:64896)
  • increased urine pH
    • increase in urine pH   (MGI Ref ID J:64896)
  • cardiovascular system phenotype
  • decreased mean systemic arterial blood pressure   (MGI Ref ID J:64896)
  • increased heart rate
    • atria display higher baseline heart rates   (MGI Ref ID J:108674)
    • decrease in heart rate in response to vagal nerve stimulation is slower   (MGI Ref ID J:108674)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • despite differences in behavioral responses to alcohol, no differences in blood alcohol elimination are detected   (MGI Ref ID J:79215)
    • abnormal renal water reabsorbtion
      • the rate of net fluid absorption is reduced in the proximal tubules   (MGI Ref ID J:64896)
    • acidosis
      • arterial blood pH and bicarbonate concentrations are reduced resulting in a modest but significant metabolic acidosis   (MGI Ref ID J:64896)
    • increased urine bicarbonate level
      • increase in urine bicarbonate concentration   (MGI Ref ID J:64896)
    • increased urine pH
      • increase in urine pH   (MGI Ref ID J:64896)
  • behavior/neurological phenotype
  • abnormal behavioral response to addictive substance
    • following dosing with 2.5 or 4.5 gm/kg mice regain their righting reflex faster compared to similarly treated wild-type controls   (MGI Ref ID J:79215)
    • enhanced behavioral response to addictive substance
      • mice fail to develop tolerance to alcohol induced hypothermia following multiple ethanol injection   (MGI Ref ID J:79215)
  • abnormal liquid preference
    • the concentration dependent increase in sucrose preference is more pronounced in mutants compared to wild-type controls   (MGI Ref ID J:79215)
    • however, the concentration dependent aversion to quinine is not significantly different from controls   (MGI Ref ID J:79215)
  • abnormal spatial learning
    • impaired performance during memory recall trials in a Morris water maze   (MGI Ref ID J:101815)
    • however, performance in the less stressful multiple T maze is improved compared to controls   (MGI Ref ID J:101815)
  • enhanced coordination
    • in the last trial mice spend more time on the rotarod compared to wild-type controls   (MGI Ref ID J:101815)
  • increased aggression towards males
    • unlike mice on a congenic C57BL/6J background, male mice display increased aggression   (MGI Ref ID J:67776)
    • mice display increased aggression compared to C57BL/6J mice but not compared to 129S2/SvPas mice   (MGI Ref ID J:67776)
  • increased alcohol consumption
    • mice consume more alcohol when presented with solutions containing high concentrations of alcohol (greater than 8% alcohol) compared to wild-type controls   (MGI Ref ID J:79215)
    • however, when presented with dilute solutions of alcohol (2 - 4%), alcohol consumption does not differ from controls   (MGI Ref ID J:79215)
  • increased anxiety-related response
    • increase in grooming behavior is seen in mice in an open field and during an elevated plus maze assay suggesting an increase in the amount of stress mice experience under these conditions   (MGI Ref ID J:101815)
  • increased coping response
    • in a forced swim test mice spend more time actively swimming and less time immobile compared to wild-type controls   (MGI Ref ID J:101815)
  • increased grooming behavior
    • seen in mice in an open field and during an elevated plus maze assay suggesting an increase in the amount of stress mice experience under these conditions   (MGI Ref ID J:101815)
  • digestive/alimentary phenotype
  • *normal* digestive/alimentary phenotype
    • unlike in human patients with Infantile Hypertrophic Pyloric Stenosis, the luminal aperture of the pyloric sphincter is not significantly smaller in relaxed tissues compared to wild type controls   (MGI Ref ID J:64225)
    • abnormal digestion
      • stomachs often contain bezoars even after 2 days of fasting   (MGI Ref ID J:64225)
      • gastric emptying of solids and liquids is delayed   (MGI Ref ID J:64225)
    • abnormal duodenum morphology
      • diffuse enlargement   (MGI Ref ID J:64225)
    • enlarged esophagus
      • diffuse enlargement   (MGI Ref ID J:64225)
    • enlarged stomach
      • diffuse enlargement   (MGI Ref ID J:64225)
      • weight and volume are increased   (MGI Ref ID J:64225)
      • gastric hypertrophy
        • muscular thickening is seen throughout the stomach   (MGI Ref ID J:64225)
    • pyloric sphincter hypertrophy
      • pyloric sphincter thickness is increased by 17% compared to controls   (MGI Ref ID J:64225)
  • muscle phenotype
  • pyloric sphincter hypertrophy
    • pyloric sphincter thickness is increased by 17% compared to controls   (MGI Ref ID J:64225)

Nos1tm1Plh/Nos1tm1Plh

        involves: 129S4/SvJae * C57BL/10ScSn
  • muscle phenotype
  • *normal* muscle phenotype
    • no abnormalities in muscle morphology are detected   (MGI Ref ID J:48851)

Nos1tm1Plh/Nos1tm1Plh

        B6;129S4-Nos1tm1Plh/J
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • increased aggressive behavior is not seen in males unlike in other reports   (MGI Ref ID J:83571)
    • abnormal sleep pattern
      • mice spend less time in REM sleep as a result of fewer REM episodes and lengthened duration of the inter REM intervals   (MGI Ref ID J:83571)
      • however, there is no significant difference in the amount of time spent in non-REM sleep and mice display normal diurnal variation in sleep patterns   (MGI Ref ID J:83571)
  • nervous system phenotype
  • abnormal brain wave pattern
    • during non-REM sleep the absolute value of slow wave activity is increased   (MGI Ref ID J:83571)
  • skeleton phenotype
  • abnormal skeleton development
    • indices of bone formation and resorption are lower   (MGI Ref ID J:105612)
    • abnormal osteoclast differentiation
      • in culture RANKL and M-CSF induced osteoclast formation are increased   (MGI Ref ID J:105612)
  • abnormal trabecular bone morphology
    • trabecular bone tends to extend deeper into the metaphysis   (MGI Ref ID J:105612)
    • increased trabecular bone thickness   (MGI Ref ID J:105612)
    • increased trabecular bone volume   (MGI Ref ID J:105612)
  • increased bone mineral content
    • bone mineral content is increased   (MGI Ref ID J:105612)
  • increased bone mineral density
    • bone mineral density is increased   (MGI Ref ID J:105612)
  • hematopoietic system phenotype
  • abnormal osteoclast differentiation
    • in culture RANKL and M-CSF induced osteoclast formation are increased   (MGI Ref ID J:105612)
  • immune system phenotype
  • abnormal osteoclast differentiation
    • in culture RANKL and M-CSF induced osteoclast formation are increased   (MGI Ref ID J:105612)
  • cellular phenotype
  • abnormal osteoclast differentiation
    • in culture RANKL and M-CSF induced osteoclast formation are increased   (MGI Ref ID J:105612)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Nos1tm1Plh related

Diabetes and Obesity Research
Insulin Resistance

Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nos1tm1Plh
Allele Name targeted mutation 1, Paul L Huang
Allele Type Targeted (knock-out)
Common Name(s) Kn; N2KO; NOSdelta; alphaNOS1; nNOS KO; nNOS-; nNOS-;
Mutation Made ByDr. Paul Huang,   Mass General Hospital, Harvard Med Sch
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Nos1, nitric oxide synthase 1, neuronal
Chromosome 5
Gene Common Name(s) 2310005C01Rik; IHPS1; N-NOS; NC-NOS; NO; NOS; Nos-1; RIKEN cDNA 2310005C01 gene; bNOS; nNOS; nitric oxide synthase 1 (macrophage);
General Note Mice homozygous for Nos1tm1Plhand Nos3tm1Plh display Phenotypic Similarity to Human Syndrome: hypertensive hypertrophic cardiomyopathy of the elderly (J:129064)
Molecular Note A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Northern blots of brain homogenates from homozygous mutant mice showed no detectable mRNA. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. [MGI Ref ID J:16390] [MGI Ref ID J:31846] [MGI Ref ID J:62375]

Genotyping

Genotyping Information

Genotyping Protocols

Nos1tm1Plh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Huang PL; Dawson TM; Bredt DS; Snyder SH; Fishman MC. 1993. Targeted disruption of the neuronal nitric oxide synthase gene. Cell 75(7):1273-86. [PubMed: 7505721]  [MGI Ref ID J:16390]

Additional References

Barouch LA; Harrison RW; Skaf MW; Rosas GO; Cappola TP; Kobeissi ZA; Hobai IA; Lemmon CA; Burnett AL; O'Rourke B; Rodriguez ER; Huang PL; Lima JA; Berkowitz DE; Hare JM. 2002. Nitric oxide regulates the heart by spatial confinement of nitric oxide synthase isoforms. Nature 416(6878):337-9. [PubMed: 11907582]  [MGI Ref ID J:75645]

Shankar RR; Wu Y; Shen HQ; Zhu JS; Baron AD. 2000. Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance. Diabetes 49(5):684-7. [PubMed: 10905473]  [MGI Ref ID J:62229]

Tao F; Tao YX; Zhao C; Dore S; Liaw WJ; Raja SN; Johns RA. 2004. Differential roles of neuronal and endothelial nitric oxide synthases during carrageenan-induced inflammatory hyperalgesia. Neuroscience 128(2):421-30. [PubMed: 15350652]  [MGI Ref ID J:93612]

de Jonge WJ; Hallemeesch MM; Kwikkers KL; Ruijter JM; de Gier-de Vries C; van Roon MA; Meijer AJ; Marescau B; de Deyn PP; Deutz NE; Lamers WH. 2002. Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development. Am J Clin Nutr 76(1):128-40. [PubMed: 12081826]  [MGI Ref ID J:80556]

Nos1tm1Plh related

Addabbo F; Ratliff B; Park HC; Kuo MC; Ungvari Z; Ciszar A; Krasnikof B; Sodhi K; Zhang F; Nasjletti A; Goligorsky MS. 2009. The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach. Am J Pathol 174(1):34-43. [PubMed: 19095954]  [MGI Ref ID J:144210]

Andersen YS; Gillin FD; Eckmann L. 2006. Adaptive immunity-dependent intestinal hypermotility contributes to host defense against Giardia spp. Infect Immun 74(4):2473-6. [PubMed: 16552082]  [MGI Ref ID J:107409]

Anderson JE. 2000. A role for nitric oxide in muscle repair: nitric oxide-mediated activation of muscle satellite cells. Mol Biol Cell 11(5):1859-74. [PubMed: 10793157]  [MGI Ref ID J:120497]

Ando A; Yang A; Mori K; Yamada H; Yamada E; Takahashi K; Saikia J; Kim M; Melia M; Fishman M; Huang P; Campochiaro PA. 2002. Nitric oxide is proangiogenic in the retina and choroid. J Cell Physiol 191(1):116-24. [PubMed: 11920687]  [MGI Ref ID J:106207]

Ashley EA; Sears CE; Bryant SM; Watkins HC; Casadei B. 2002. Cardiac nitric oxide synthase 1 regulates basal and beta-adrenergic contractility in murine ventricular myocytes. Circulation 105(25):3011-6. [PubMed: 12081996]  [MGI Ref ID J:103346]

Ayata C; Ayata G; Hara H; Matthews RT; Beal MF; Ferrante RJ ; Endres M ; Kim A ; Christie RH ; Waeber C ; Huang PL ; Hyman BT ; Moskowitz MA. 1997. Mechanisms of reduced striatal NMDA excitotoxicity in type I nitric oxide synthase knock-out mice. J Neurosci 17(18):6908-17. [PubMed: 9278526]  [MGI Ref ID J:42885]

Barouch LA; Cappola TP; Harrison RW; Crone JK; Rodriguez ER; Burnett AL; Hare JM. 2003. Combined loss of neuronal and endothelial nitric oxide synthase causes premature mortality and age-related hypertrophic cardiac remodeling in mice. J Mol Cell Cardiol 35(6):637-44. [PubMed: 12788381]  [MGI Ref ID J:129064]

Barouch LA; Harrison RW; Skaf MW; Rosas GO; Cappola TP; Kobeissi ZA; Hobai IA; Lemmon CA; Burnett AL; O'Rourke B; Rodriguez ER; Huang PL; Lima JA; Berkowitz DE; Hare JM. 2002. Nitric oxide regulates the heart by spatial confinement of nitric oxide synthase isoforms. Nature 416(6878):337-9. [PubMed: 11907582]  [MGI Ref ID J:75645]

Baum O; Vieregge M; Koch P; Gul S; Hahn S; Huber-Abel FA; Pries AR; Hoppeler H. 2013. Phenotype of capillaries in skeletal muscle of nNOS-knockout mice. Am J Physiol Regul Integr Comp Physiol 304(12):R1175-82. [PubMed: 23576613]  [MGI Ref ID J:198523]

Beck PL; Xavier R; Wong J; Ezedi I; Mashimo H; Mizoguchi A; Mizoguchi E; Bhan AK; Podolsky DK. 2004. Paradoxical roles of different nitric oxide synthase isoforms in colonic injury. Am J Physiol Gastrointest Liver Physiol 286(1):G137-47. [PubMed: 14665440]  [MGI Ref ID J:87601]

Bernstein HG; Keilhoff G; Seidel B; Stanarius A; Huang PL; Fishman MC; Reiser M; Bogerts B; Wolf G. 1998. Expression of hypothalamic peptides in mice lacking neuronal nitric oxide synthase: reduced beta-END immunoreactivity in the arcuate nucleus. Neuroendocrinology 68(6):403-11. [PubMed: 9873204]  [MGI Ref ID J:51911]

Bilbo SD; Hotchkiss AK; Chiavegatto S; Nelson RJ. 2003. Blunted stress responses in delayed type hypersensitivity in mice lacking the neuronal isoform of nitric oxide synthase. J Neuroimmunol 140(1-2):41-8. [PubMed: 12864970]  [MGI Ref ID J:119000]

Bonthius DJ; Tzouras G; Karacay B; Mahoney J; Hutton A; McKim R; Pantazis NJ. 2002. Deficiency of neuronal nitric oxide synthase (nNOS) worsens alcohol-induced microencephaly and neuronal loss in developing mice. Brain Res Dev Brain Res 138(1):45-59. [PubMed: 12234657]  [MGI Ref ID J:79331]

Brenman JE; Chao DS; Gee SH; McGee AW; Craven SE; Santillano DR; Wu Z; Huang F; Xia H; Peters MF; Froehner SC; Bredt DS. 1996. Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains. Cell 84(5):757-67. [PubMed: 8625413]  [MGI Ref ID J:31846]

Browne SE; Ayata C; Huang PL; Moskowitz MA; Beal MF. 1999. The cerebral metabolic consequences of nitric oxide synthase deficiency: glucose utilization in endothelial and neuronal nitric oxide synthase null mice. J Cereb Blood Flow Metab 19(2):144-8. [PubMed: 10027769]  [MGI Ref ID J:54728]

Burger DE; Lu X; Lei M; Xiang FL; Hammoud L; Jiang M; Wang H; Jones DL; Sims SM; Feng Q. 2009. Neuronal nitric oxide synthase protects against myocardial infarction-induced ventricular arrhythmia and mortality in mice. Circulation 120(14):1345-54. [PubMed: 19770398]  [MGI Ref ID J:167497]

Burnett AL; Calvin DC; Chamness SL; Liu JX; Nelson RJ; Klein SL ; Dawson VL ; Dawson TM ; Snyder SH. 1997. Urinary bladder-urethral sphincter dysfunction in mice with targeted disruption of neuronal nitric oxide synthase models idiopathic voiding disorders in humans. Nat Med 3(5):571-4. [PubMed: 9142130]  [MGI Ref ID J:40184]

Burnett AL; Nelson RJ; Calvin DC; Liu JX; Demas GE; Klein SL; Kriegsfeld LJ; Dawson VL; Dawson TM; Snyder SH. 1996. Nitric oxide-dependent penile erection in mice lacking neuronal nitric oxide synthase. Mol Med 2(3):288-96. [PubMed: 8784782]  [MGI Ref ID J:113071]

Calingasan NY; Huang PL; Chun HS; Fabian A; Gibson GE. 2000. Vascular factors are critical in selective neuronal loss in an animal model of impaired oxidative metabolism. J Neuropathol Exp Neurol 59(3):207-17. [PubMed: 10744059]  [MGI Ref ID J:62409]

Castrop H; Schweda F; Mizel D; Huang Y; Briggs J; Kurtz A; Schnermann J. 2004. Permissive role of nitric oxide in macula densa control of renin secretion. Am J Physiol Renal Physiol 286(5):F848-57. [PubMed: 15075180]  [MGI Ref ID J:113763]

Champion HC; Bivalacqua TJ; Takimoto E; Kass DA; Burnett AL. 2005. Phosphodiesterase-5A dysregulation in penile erectile tissue is a mechanism of priapism. Proc Natl Acad Sci U S A 102(5):1661-6. [PubMed: 15668387]  [MGI Ref ID J:96103]

Chandrasekharan B; Bala V; Kolachala VL; Vijay-Kumar M; Jones D; Gewirtz AT; Sitaraman SV; Srinivasan S. 2008. Targeted deletion of neuropeptide Y (NPY) modulates experimental colitis. PLoS ONE 3(10):e3304. [PubMed: 18836554]  [MGI Ref ID J:144421]

Chao DS; Silvagno F; Bredt DS. 1998. Muscular dystrophy in mdx mice despite lack of neuronal nitric oxide synthase. J Neurochem 71(2):784-9. [PubMed: 9681470]  [MGI Ref ID J:48851]

Chen G; Dunbar RL; Gao W; Ebner TJ. 2001. Role of calcium, glutamate neurotransmission, and nitric oxide in spreading acidification and depression in the cerebellar cortex. J Neurosci 21(24):9877-87. [PubMed: 11739595]  [MGI Ref ID J:73381]

Chen J; Tu Y; Moon C; Matarazzo V; Palmer AM; Ronnett GV. 2004. The localization of neuronal nitric oxide synthase may influence its role in neuronal precursor proliferation and synaptic maintenance. Dev Biol 269(1):165-82. [PubMed: 15081365]  [MGI Ref ID J:106231]

Chen L; Majde JA; Krueger JM. 2003. Spontaneous sleep in mice with targeted disruptions of neuronal or inducible nitric oxide synthase genes. Brain Res 973(2):214-22. [PubMed: 12738065]  [MGI Ref ID J:83571]

Chen L; Taishi P; Duricka D; Krueger JM. 2004. Brainstem prolactin mRNA is enhanced in mice with suppressed neuronal nitric oxide synthase activity. Brain Res Mol Brain Res 129(1-2):179-84. [PubMed: 15469894]  [MGI Ref ID J:115454]

Chen L; Taishi P; Majde JA; Peterfi Z; Obal F Jr; Krueger JM. 2004. The role of nitric oxide synthases in the sleep responses to tumor necrosis factor-alpha. Brain Behav Immun 18(4):390-8. [PubMed: 15157956]  [MGI Ref ID J:105452]

Chiavegatto S; Dawson VL; Mamounas LA; Koliatsos VE; Dawson TM; Nelson RJ. 2001. Brain serotonin dysfunction accounts for aggression in male mice lacking neuronal nitric oxide synthase. Proc Natl Acad Sci U S A 98(3):1277-81. [PubMed: 11158630]  [MGI Ref ID J:127032]

Choate JK; Danson EJ; Morris JF; Paterson DJ. 2001. Peripheral vagal control of heart rate is impaired in neuronal NOS knockout mice. Am J Physiol Heart Circ Physiol 281(6):H2310-7. [PubMed: 11709397]  [MGI Ref ID J:108674]

Choate JK; Murphy SM; Feldman R; Anderson CR. 2008. Sympathetic control of heart rate in nNOS knockout mice. Am J Physiol Heart Circ Physiol 294(1):H354-61. [PubMed: 17951372]  [MGI Ref ID J:132453]

Chu YC; Guan Y; Skinner J; Raja SN; Johns RA; Tao YX. 2005. Effect of genetic knockout or pharmacologic inhibition of neuronal nitric oxide synthase on complete Freund's adjuvant-induced persistent pain. Pain 119(1-3):113-23. [PubMed: 16297560]  [MGI Ref ID J:106376]

Chung YH; Joo KM; Nam RH; Lee WB; Lee KH; Cha CI. 2004. Region-specific alterations in insulin-like growth factor-I receptor in the central nervous system of nNOS knockout mice. Brain Res 1021(1):132-9. [PubMed: 15328041]  [MGI Ref ID J:107781]

Church JE; Gehrig SM; Chee A; Naim T; Trieu J; McConell GK; Lynch GS. 2011. Early functional muscle regeneration after myotoxic injury in mice is unaffected by nNOS absence. Am J Physiol Regul Integr Comp Physiol 301(5):R1358-66. [PubMed: 21849632]  [MGI Ref ID J:178806]

Crosbie RH; Straub V; Yun HY; Lee JC; Rafael JA; Chamberlain JS; Dawson VL; Dawson TM; Campbell KP. 1998. mdx muscle pathology is independent of nNOS perturbation. Hum Mol Genet 7(5):823-9. [PubMed: 9536086]  [MGI Ref ID J:47623]

Da Silva-Azevedo L; Jahne S; Hoffmann C; Stalder D; Heller M; Pries AR; Zakrzewicz A; Baum O. 2009. Up-regulation of the peroxiredoxin-6 related metabolism of reactive oxygen species in skeletal muscle of mice lacking neuronal nitric oxide synthase. J Physiol 587(Pt 3):655-68. [PubMed: 19047200]  [MGI Ref ID J:176558]

Dachtler J; Hardingham NR; Fox K. 2012. The role of nitric oxide synthase in cortical plasticity is sex specific. J Neurosci 32(43):14994-9. [PubMed: 23100421]  [MGI Ref ID J:191221]

Danson EJ; Mankia KS; Golding S; Dawson T; Everatt L; Cai S; Channon KM; Paterson DJ. 2004. Impaired regulation of neuronal nitric oxide synthase and heart rate during exercise in mice lacking one nNOS allele. J Physiol 558(Pt 3):963-74. [PubMed: 15155789]  [MGI Ref ID J:105579]

Dautzenberg M; Keilhoff G; Just A. 2011. Modulation of the myogenic response in renal blood flow autoregulation by NO depends on endothelial nitric oxide synthase (eNOS), but not neuronal or inducible NOS. J Physiol 589(Pt 19):4731-44. [PubMed: 21825026]  [MGI Ref ID J:189387]

Dawson D; Lygate CA; Zhang MH; Hulbert K; Neubauer S; Casadei B. 2005. nNOS gene deletion exacerbates pathological left ventricular remodeling and functional deterioration after myocardial infarction. Circulation 112(24):3729-37. [PubMed: 16344403]  [MGI Ref ID J:116873]

Demchenko IT; Atochin DN; Boso AE; Astern J; Huang PL; Piantadosi CA. 2003. Oxygen seizure latency and peroxynitrite formation in mice lacking neuronal or endothelial nitric oxide synthases. Neurosci Lett 344(1):53-6. [PubMed: 12781920]  [MGI Ref ID J:107964]

Demchenko IT; Atochin DN; Gutsaeva DR; Godfrey RR; Huang PL; Piantadosi CA; Allen BW. 2008. Contributions of nitric oxide synthase isoforms to pulmonary oxygen toxicity, local vs. mediated effects. Am J Physiol Lung Cell Mol Physiol 294(5):L984-90. [PubMed: 18326824]  [MGI Ref ID J:136632]

Deng B; Glanzman D; Tidball JG. 2009. Nitric oxide generated by muscle corrects defects in hippocampal neurogenesis and neural differentiation caused by muscular dystrophy. J Physiol 587(Pt 8):1769-78. [PubMed: 19237426]  [MGI Ref ID J:176544]

DiMagno MJ; Hao Y; Tsunoda Y; Williams JA; Owyang C. 2004. Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice. Am J Physiol Gastrointest Liver Physiol 286(3):G428-36. [PubMed: 14551061]  [MGI Ref ID J:95674]

Diesen DL; Hess DT; Stamler JS. 2008. Hypoxic vasodilation by red blood cells: evidence for an s-nitrosothiol-based signal. Circ Res 103(5):545-53. [PubMed: 18658051]  [MGI Ref ID J:152645]

Eliasson MJ; Huang Z; Ferrante RJ; Sasamata M; Molliver ME; Snyder SH; Moskowitz MA. 1999. Neuronal nitric oxide synthase activation and peroxynitrite formation in ischemic stroke linked to neural damage. J Neurosci 19(14):5910-8. [PubMed: 10407030]  [MGI Ref ID J:119782]

Elibol B; Soylemezoglu F; Unal I; Fujii M; Hirt L; Huang PL; Moskowitz MA; Dalkara T. 2001. Nitric oxide is involved in ischemia-induced apoptosis in brain: a study in neuronal nitric oxide synthase null mice. Neuroscience 105(1):79-86. [PubMed: 11483302]  [MGI Ref ID J:126861]

Endres M; Scott G; Namura S; Salzman AL; Huang PL; Moskowitz MA; Szabo C. 1998. Role of peroxynitrite and neuronal nitric oxide synthase in the activation of poly(ADP-ribose) synthetase in a murine model of cerebral ischemia-reperfusion. Neurosci Lett 248(1):41-4. [PubMed: 9665659]  [MGI Ref ID J:107969]

Facchinetti F; Sasaki M; Cutting FB; Zhai P; MacDonald JE; Reif D; Beal MF; Huang PL; Dawson TM; Gurney ME; Dawson VL. 1999. Lack of involvement of neuronal nitric oxide synthase in the pathogenesis of a transgenic mouse model of familial amyotrophic lateral sclerosis. Neuroscience 90(4):1483-92. [PubMed: 10338314]  [MGI Ref ID J:57196]

Fagan KA; Tyler RC; Sato K; Fouty BW; Morris KG Jr; Huang PL; McMurtry IF; Rodman DM. 1999. Relative contributions of endothelial, inducible, and neuronal NOS to tone in the murine pulmonary circulation. Am J Physiol 277(3 Pt 1):L472-8. [PubMed: 10484454]  [MGI Ref ID J:57624]

Finney EM; Shatz CJ. 1998. Establishment of patterned thalamocortical connections does not require nitric oxide synthase. J Neurosci 18(21):8826-38. [PubMed: 9786989]  [MGI Ref ID J:112195]

Fioramonti X; Marsollier N; Song Z; Fakira KA; Patel RM; Brown S; Duparc T; Pica-Mendez A; Sanders NM; Knauf C; Valet P; McCrimmon RJ; Beuve A; Magnan C; Routh VH. 2010. Ventromedial hypothalamic nitric oxide production is necessary for hypoglycemia detection and counterregulation. Diabetes 59(2):519-28. [PubMed: 19934009]  [MGI Ref ID J:164156]

Fritzen S; Schmitt A; Koth K; Sommer C; Lesch KP; Reif A. 2007. Neuronal nitric oxide synthase (NOS-I) knockout increases the survival rate of neural cells in the hippocampus independently of BDNF. Mol Cell Neurosci 35(2):261-71. [PubMed: 17459722]  [MGI Ref ID J:123224]

Gammie SC; Nelson RJ. 1999. Maternal aggression is reduced in neuronal nitric oxide synthase-deficient mice. J Neurosci 19(18):8027-35. [PubMed: 10479702]  [MGI Ref ID J:57543]

Girouard H; Wang G; Gallo EF; Anrather J; Zhou P; Pickel VM; Iadecola C. 2009. NMDA receptor activation increases free radical production through nitric oxide and NOX2. J Neurosci 29(8):2545-52. [PubMed: 19244529]  [MGI Ref ID J:145943]

Gonzalez DR; Beigi F; Treuer AV; Hare JM. 2007. Deficient ryanodine receptor S-nitrosylation increases sarcoplasmic reticulum calcium leak and arrhythmogenesis in cardiomyocytes. Proc Natl Acad Sci U S A 104(51):20612-7. [PubMed: 18077344]  [MGI Ref ID J:141533]

Grange RW; Isotani E; Lau KS; Kamm KE; Huang PL; Stull JT. 2001. Nitric oxide contributes to vascular smooth muscle relaxation in contracting fast-twitch muscles. Physiol Genomics 5(1):35-44. [PubMed: 11161004]  [MGI Ref ID J:124464]

Greenberg SS; Ouyang J; Zhao X; Parrish C; Nelson S; Giles TD. 1999. Effects of ethanol on neutrophil recruitment and lung host defense in nitric oxide synthase I and nitric oxide synthase II knockout mice. Alcohol Clin Exp Res 23(9):1435-45. [PubMed: 10512307]  [MGI Ref ID J:59745]

Gunawardana SC; Rocheleau JV; Head WS; Piston DW. 2006. Mechanisms of time-dependent potentiation of insulin release: involvement of nitric oxide synthase. Diabetes 55(4):1029-33. [PubMed: 16567525]  [MGI Ref ID J:108613]

Gutsaeva DR; Carraway MS; Suliman HB; Demchenko IT; Shitara H; Yonekawa H; Piantadosi CA. 2008. Transient hypoxia stimulates mitochondrial biogenesis in brain subcortex by a neuronal nitric oxide synthase-dependent mechanism. J Neurosci 28(9):2015-24. [PubMed: 18305236]  [MGI Ref ID J:149338]

Hanchate NK; Parkash J; Bellefontaine N; Mazur D; Colledge WH; d'Anglemont de Tassigny X; Prevot V. 2012. Kisspeptin-GPR54 Signaling in Mouse NO-Synthesizing Neurons Participates in the Hypothalamic Control of Ovulation. J Neurosci 32(3):932-45. [PubMed: 22262891]  [MGI Ref ID J:179891]

Hao M; Head WS; Gunawardana SC; Hasty AH; Piston DW. 2007. Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction. Diabetes 56(9):2328-38. [PubMed: 17575085]  [MGI Ref ID J:126584]

Harding P; Sigmon DH; Alfie ME; Huang PL; Fishman MC; Beierwaltes WH; Carretero OA. 1997. Cyclooxygenase-2 mediates increased renal renin content induced by low-sodium diet. Hypertension 29(1 Pt 2):297-302. [PubMed: 9039118]  [MGI Ref ID J:111201]

Hervera A; Negrete R; Leanez S; Martin-Campos JM; Pol O. 2010. The spinal cord expression of neuronal and inducible nitric oxide synthases and their contribution in the maintenance of neuropathic pain in mice. PLoS One 5(12):e14321. [PubMed: 21179208]  [MGI Ref ID J:168092]

Hoang T; Choi DK; Nagai M; Wu DC; Nagata T; Prou D; Wilson GL; Vila M; Jackson-Lewis V; Dawson VL; Dawson TM; Chesselet MF; Przedborski S. 2009. Neuronal NOS and cyclooxygenase-2 contribute to DNA damage in a mouse model of Parkinson disease. Free Radic Biol Med 47(7):1049-56. [PubMed: 19616617]  [MGI Ref ID J:152538]

Hoogerwerf WA; Shahinian VB; Cornelissen G; Halberg F; Bostwick J; Timm J; Bartell PA; Cassone VM. 2010. Rhythmic changes in colonic motility are regulated by period genes. Am J Physiol Gastrointest Liver Physiol 298(2):G143-50. [PubMed: 19926812]  [MGI Ref ID J:157491]

Hu M; Sun YJ; Zhou QG; Chen L; Hu Y; Luo CX; Wu JY; Xu JS; Li LX; Zhu DY. 2008. Negative regulation of neurogenesis and spatial memory by NR2B-containing NMDA receptors. J Neurochem 106(4):1900-13. [PubMed: 18624924]  [MGI Ref ID J:141852]

Hu Y; Wu DL; Luo CX; Zhu LJ; Zhang J; Wu HY; Zhu DY. 2012. Hippocampal nitric oxide contributes to sex difference in affective behaviors. Proc Natl Acad Sci U S A 109(35):14224-9. [PubMed: 22891311]  [MGI Ref ID J:188583]

Huang Z; Huang PL; Panahian N; Dalkara T; Fishman MC; Moskowitz MA. 1994. Effects of cerebral ischemia in mice deficient in neuronal nitric oxide synthase. Science 265(5180):1883-5. [PubMed: 7522345]  [MGI Ref ID J:127062]

Hurt KJ; Musicki B; Palese MA; Crone JK; Becker RE; Moriarity JL; Snyder SH; Burnett AL. 2002. Akt-dependent phosphorylation of endothelial nitric-oxide synthase mediates penile erection. Proc Natl Acad Sci U S A 99(6):4061-6. [PubMed: 11904450]  [MGI Ref ID J:89704]

Hurt KJ; Sezen SF; Champion HC; Crone JK; Palese MA; Huang PL; Sawa A; Luo X; Musicki B; Snyder SH; Burnett AL. 2006. Alternatively spliced neuronal nitric oxide synthase mediates penile erection. Proc Natl Acad Sci U S A 103(9):3440-3. [PubMed: 16488973]  [MGI Ref ID J:107168]

Hurt KJ; Sezen SF; Lagoda GF; Musicki B; Rameau GA; Snyder SH; Burnett AL. 2012. Cyclic AMP-dependent phosphorylation of neuronal nitric oxide synthase mediates penile erection. Proc Natl Acad Sci U S A 109(41):16624-9. [PubMed: 23012472]  [MGI Ref ID J:190323]

Hussain MB; Hobbs AJ; MacAllister RJ. 1999. Autoregulation of nitric oxide-soluble guanylate cyclase-cyclic GMP signalling in mouse thoracic aorta. Br J Pharmacol 128(5):1082-8. [PubMed: 10556946]  [MGI Ref ID J:59681]

Hyndman KA; Boesen EI; Elmarakby AA; Brands MW; Huang P; Kohan DE; Pollock DM; Pollock JS. 2013. Renal collecting duct NOS1 maintains fluid-electrolyte homeostasis and blood pressure. Hypertension 62(1):91-8. [PubMed: 23608660]  [MGI Ref ID J:202017]

Idigo WO; Reilly S; Zhang MH; Zhang YH; Jayaram R; Carnicer R; Crabtree MJ; Balligand JL; Casadei B. 2012. Regulation of endothelial nitric-oxide synthase (NOS) S-glutathionylation by neuronal NOS: evidence of a functional interaction between myocardial constitutive NOS isoforms. J Biol Chem 287(52):43665-73. [PubMed: 23091050]  [MGI Ref ID J:193414]

Iijima H; Tulic MK; Duguet A; Shan J; Carbonara P; Hamid Q; Eidelman DH. 2005. NOS 1 is required for allergen-induced expression of NOS 2 in mice. Int Arch Allergy Immunol 138(1):40-50. [PubMed: 16103686]  [MGI Ref ID J:115740]

Imam SZ; Newport GD; Itzhak Y; Cadet JL; Islam F; Slikker W; Ali SF. 2001. Peroxynitrite plays a role in methamphetamine-induced dopaminergic neurotoxicity: evidence from mice lacking neuronal nitric oxide synthase gene or overexpressing copper-zinc superoxide dismutase. J Neurochem 76(3):745-9. [PubMed: 11158245]  [MGI Ref ID J:67775]

Inglis FM; Furia F; Zuckerman KE; Strittmatter SM; Kalb RG. 1998. The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites. J Neurosci 18(24):10493-501. [PubMed: 9852587]  [MGI Ref ID J:111340]

Itoh K; Watanabe M. 2009. Paradoxical facilitation of pentylenetetrazole-induced convulsion susceptibility in mice lacking neuronal nitric oxide synthase. Neuroscience 159(2):735-43. [PubMed: 19162139]  [MGI Ref ID J:148964]

Juch M; Smalla KH; Kahne T; Lubec G; Tischmeyer W; Gundelfinger ED; Engelmann M. 2009. Congenital lack of nNOS impairs long-term social recognition memory and alters the olfactory bulb proteome. Neurobiol Learn Mem 92(4):469-84. [PubMed: 19531381]  [MGI Ref ID J:154408]

Jumrussirikul P; Dinerman J; Dawson TM; Dawson VL; Ekelund U; Georgakopoulos D; Schramm LP; Calkins H; Snyder SH; Hare JM; Berger RD. 1998. Interaction between neuronal nitric oxide synthase and inhibitory G protein activity in heart rate regulation in conscious mice. J Clin Invest 102(7):1279-85. [PubMed: 9769319]  [MGI Ref ID J:115244]

Kanai AJ; Pearce LL; Clemens PR; Birder LA; VanBibber MM; Choi SY; de Groat WC; Peterson J. 2001. Identification of a neuronal nitric oxide synthase in isolated cardiac mitochondria using electrochemical detection. Proc Natl Acad Sci U S A 98(24):14126-31. [PubMed: 11717466]  [MGI Ref ID J:125463]

Kano T; Shimizu-Sasamata M; Huang PL; Moskowitz MA; Lo EH. 1998. Effects of nitric oxide synthase gene knockout on neurotransmitter release in vivo. Neuroscience 86(3):695-9. [PubMed: 9692709]  [MGI Ref ID J:119052]

Karacay B; Li G; Pantazis NJ; Bonthius DJ. 2007. Stimulation of the cAMP pathway protects cultured cerebellar granule neurons against alcohol-induced cell death by activating the neuronal nitric oxide synthase (nNOS) gene. Brain Res 1143:34-45. [PubMed: 17306238]  [MGI Ref ID J:121468]

Katoh A; Kitazawa H; Itohara S; Nagao S. 2000. Inhibition of nitric oxide synthesis and gene knockout of neuronal nitric oxide synthase impaired adaptation of mouse optokinetic response eye movements. Learn Mem 7(4):220-6. [PubMed: 10940322]  [MGI Ref ID J:103910]

Keilhoff G; Fansa H; Wolf G. 2002. Differences in peripheral nerve degeneration/regeneration between wild-type and neuronal nitric oxide synthase knockout mice. J Neurosci Res 68(4):432-41. [PubMed: 11992469]  [MGI Ref ID J:104916]

Keilhoff G; Seidel B; Reiser M; Stanarius A; Huang PL; Bogerts B; Wolf G; Bernstein HG. 2001. Lack of neuronal NOS has consequences for the expression of POMC and POMC-derived peptides in the mouse pituitary. Acta Histochem 103(4):397-412. [PubMed: 11700945]  [MGI Ref ID J:102572]

Keilhoff G; Wolf G; Bernstein H. 2001. Altered laminar distribution of hippocampal zinc in mutant mice lacking neuronal nitric oxide synthase. A histochemical study. Neurosci Lett 305(3):173-6. [PubMed: 11403933]  [MGI Ref ID J:108030]

Kelley JB; Anderson KL; Altmann SL; Itzhak Y. 2011. Long-term memory of visually cued fear conditioning: roles of the neuronal nitric oxide synthase gene and cyclic AMP response element-binding protein. Neuroscience 174:91-103. [PubMed: 21073925]  [MGI Ref ID J:170275]

Kelley JB; Balda MA; Anderson KL; Itzhak Y. 2009. Impairments in fear conditioning in mice lacking the nNOS gene. Learn Mem 16(6):371-8. [PubMed: 19470653]  [MGI Ref ID J:164042]

Keswani SC; Bosch-Marce M; Reed N; Fischer A; Semenza GL; Hoke A. 2011. Nitric oxide prevents axonal degeneration by inducing HIF-1-dependent expression of erythropoietin. Proc Natl Acad Sci U S A 108(12):4986-90. [PubMed: 21383158]  [MGI Ref ID J:170094]

Kim CD; Goyal RK; Mashimo H. 1999. Neuronal NOS provides nitrergic inhibitory neurotransmitter in mouse lower esophageal sphincter. Am J Physiol 277(2 Pt 1):G280-4. [PubMed: 10444441]  [MGI Ref ID J:56948]

Kim MJ; Chung YH; Joo KM; Oh GT; Kim J; Lee B; Cha CI. 2004. Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum. Neurosci Lett 369(1):39-43. [PubMed: 15380304]  [MGI Ref ID J:120019]

Kim MJ; Joo KM; Chung YH; Lee YJ; Kim J; Lee BH; Shin DH; Lee KH; Cha CI. 2003. Vasoactive intestinal peptide (VIP) and VIP mRNA decrease in the cerebral cortex of nNOS knock-out(-/-) mice. Brain Res 978(1-2):233-40. [PubMed: 12834919]  [MGI Ref ID J:86456]

Kinugawa S; Huang H; Wang Z; Kaminski PM; Wolin MS; Hintze TH. 2005. A defect of neuronal nitric oxide synthase increases xanthine oxidase-derived superoxide anion and attenuates the control of myocardial oxygen consumption by nitric oxide derived from endothelial nitric oxide synthase. Circ Res 96(3):355-62. [PubMed: 15637297]  [MGI Ref ID J:106887]

Kirchner L; Weitzdoerfer R; Hoeger H; Url A; Schmidt P; Engelmann M; Villar SR; Fountoulakis M; Lubec G; Lubec B. 2004. Impaired cognitive performance in neuronal nitric oxide synthase knockout mice is associated with hippocampal protein derangements. Nitric Oxide 11(4):316-30. [PubMed: 15604044]  [MGI Ref ID J:101988]

Kitaura H; Uozumi N; Tohmi M; Yamazaki M; Sakimura K; Kudoh M; Shimizu T; Shibuki K. 2007. Roles of nitric oxide as a vasodilator in neurovascular coupling of mouse somatosensory cortex. Neurosci Res 59(2):160-71. [PubMed: 17655958]  [MGI Ref ID J:136589]

Klein SL; Carnovale D; Burnett AL; Wallach EE; Zacur HA; Crone JK; Dawson VL; Nelson RJ; Dawson TM. 1998. Impaired ovulation in mice with targeted deletion of the neuronal isoform of nitric oxide synthase. Mol Med 4(10):658-64. [PubMed: 9848082]  [MGI Ref ID J:89701]

Kline DD; Overholt JL; Prabhakar NR. 2002. Mutant mice deficient in NOS-1 exhibit attenuated long-term facilitation and short-term potentiation in breathing. J Physiol 539(Pt 1):309-15. [PubMed: 11850522]  [MGI Ref ID J:105969]

Kline DD; Yang T; Huang PL; Prabhakar NR. 1998. Altered respiratory responses to hypoxia in mutant mice deficient in neuronal nitric oxide synthase. J Physiol 511(Pt 1):273-87. [PubMed: 9679181]  [MGI Ref ID J:106328]

Kobayashi YM; Rader EP; Crawford RW; Iyengar NK; Thedens DR; Faulkner JA; Parikh SV; Weiss RM; Chamberlain JS; Moore SA; Campbell KP. 2008. Sarcolemma-localized nNOS is required to maintain activity after mild exercise. Nature 456(7221):511-5. [PubMed: 18953332]  [MGI Ref ID J:144084]

Koliatsos VE; Dawson TM; Kecojevic A; Zhou Y; Wang YF; Huang KX. 2004. Cortical interneurons become activated by deafferentation and instruct the apoptosis of pyramidal neurons. Proc Natl Acad Sci U S A 101(39):14264-9. [PubMed: 15381772]  [MGI Ref ID J:93439]

Kovacs R; Rabanus A; Otahal J; Patzak A; Kardos J; Albus K; Heinemann U; Kann O. 2009. Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices. J Neurosci 29(26):8565-77. [PubMed: 19571147]  [MGI Ref ID J:150804]

Kozak W; Kozak A. 2003. Genetic Models in Applied Physiology. Differential role of nitric oxide synthase isoforms in fever of different etiologies: studies using Nos gene-deficient mice. J Appl Physiol 94(6):2534-44. [PubMed: 12562678]  [MGI Ref ID J:103018]

Kriegsfeld LJ; Eliasson MJ; Demas GE; Blackshaw S; Dawson TM; Nelson RJ; Snyder SH. 1999. Nocturnal motor coordination deficits in neuronal nitric oxide synthase knock-out mice. Neuroscience 89(2):311-5. [PubMed: 10077313]  [MGI Ref ID J:56005]

Lagoda G; Sezen SF; Hurt KJ; Cabrini MR; Mohanty DK; Burnett AL. 2014. Sustained nitric oxide (NO)-releasing compound reverses dysregulated NO signal transduction in priapism. FASEB J 28(1):76-84. [PubMed: 24076963]  [MGI Ref ID J:206623]

Lau KS; Grange RW; Isotani E; Sarelius IH; Kamm KE; Huang PL; Stull JT. 2000. nNOS and eNOS modulate cGMP formation and vascular response in contracting fast-twitch skeletal muscle Physiol Genomics 2(1):21-27. [PubMed: 11015578]  [MGI Ref ID J:62742]

Le Roy I; Pothion S; Mortaud S; Chabert C; Nicolas L; Cherfouh A; Roubertoux PL. 2000. Loss of aggression, after transfer onto a C57BL/6J background, in mice carrying a targeted disruption of the neuronal nitric oxide synthase gene. Behav Genet 30(5):367-73. [PubMed: 11235982]  [MGI Ref ID J:67776]

Lefer DJ; Jones SP; Girod WG; Baines A; Grisham MB; Cockrell AS; Huang PL; Scalia R. 1999. Leukocyte-endothelial cell interactions in nitric oxide synthase-deficient mice. Am J Physiol 276(6 Pt 2):H1943-50. [PubMed: 10362674]  [MGI Ref ID J:55936]

Lev-Ram V; Nebyelul Z; Ellisman MH; Huang PL; Tsien RY. 1997. Absence of cerebellar long-term depression in mice lacking neuronal nitric oxide synthase. Learn Mem 4(1):169-177. [PubMed: 10456061]  [MGI Ref ID J:42548]

Levine DZ; Iacovitti M. 2006. Real-time measurement of kidney tubule fluid nitric oxide concentrations in early diabetes: disparate changes in different rodent models. Nitric Oxide 15(1):87-92. [PubMed: 16510300]  [MGI Ref ID J:112767]

Li E; Zhou P; Singer SM. 2006. Neuronal nitric oxide synthase is necessary for elimination of Giardia lamblia infections in mice. J Immunol 176(1):516-21. [PubMed: 16365445]  [MGI Ref ID J:126256]

Li X; Clark JD. 2001. Spinal cord nitric oxide synthase and heme oxygenase limit morphine induced analgesia. Brain Res Mol Brain Res 95(1-2):96-102. [PubMed: 11687280]  [MGI Ref ID J:72814]

Li X; Nemoto M; Xu Z; Yu SW; Shimoji M; Andrabi SA; Haince JF; Poirier GG; Dawson TM; Dawson VL; Koehler RC. 2007. Influence of duration of focal cerebral ischemia and neuronal nitric oxide synthase on translocation of apoptosis-inducing factor to the nucleus. Neuroscience 144(1):56-65. [PubMed: 17049179]  [MGI Ref ID J:117941]

Lidington D; Li F; Tyml K. 2007. Deletion of neuronal NOS prevents impaired vasodilation in septic mouse skeletal muscle. Cardiovasc Res 74(1):151-8. [PubMed: 17258180]  [MGI Ref ID J:119500]

Liu X; Li C; Falck JR; Roman RJ; Harder DR; Koehler RC. 2008. Interaction of nitric oxide, 20-HETE, and EETs during functional hyperemia in whisker barrel cortex. Am J Physiol Heart Circ Physiol 295(2):H619-31. [PubMed: 18502903]  [MGI Ref ID J:138206]

Ma J; Ayata C; Huang PL; Fishman MC; Moskowitz MA. 1996. Regional cerebral blood flow response to vibrissal stimulation in mice lacking type I NOS gene expression. Am J Physiol 270(3 Pt 2):H1085-90. [PubMed: 8780207]  [MGI Ref ID J:113045]

Mang CF; Truempler S; Erbelding D; Kilbinger H. 2002. Modulation by NO of acetylcholine release in the ileum of wild-type and NOS gene knockout mice. Am J Physiol Gastrointest Liver Physiol 283(5):G1132-8. [PubMed: 12381527]  [MGI Ref ID J:108055]

Martin LJ; Chen K; Liu Z. 2005. Adult motor neuron apoptosis is mediated by nitric oxide and Fas death receptor linked by DNA damage and p53 activation. J Neurosci 25(27):6449-59. [PubMed: 16000635]  [MGI Ref ID J:99428]

Martin SR; Emanuel K; Sears CE; Zhang YH; Casadei B. 2006. Are myocardial eNOS and nNOS involved in the beta-adrenergic and muscarinic regulation of inotropy? A systematic investigation. Cardiovasc Res 70(1):97-106. [PubMed: 16545353]  [MGI Ref ID J:107830]

Mashimo H; Kjellin A; Goyal RK. 2000. Gastric stasis in neuronal nitric oxide synthase-deficient knockout mice Gastroenterology 119(3):766-73. [PubMed: 10982771]  [MGI Ref ID J:64225]

Matthews RT; Beal MF; Fallon J; Fedorchak K; Huang PL; Fishman MC; Hyman BT. 1997. MPP+ induced substantia nigra degeneration is attenuated in nNOS knockout mice. Neurobiol Dis 4(2):114-21. [PubMed: 9331901]  [MGI Ref ID J:43295]

Mattson DL; Meister CJ. 2005. Renal cortical and medullary blood flow responses to L-NAME and ANG II in wild-type, nNOS null mutant, and eNOS null mutant mice. Am J Physiol Regul Integr Comp Physiol 289(4):R991-7. [PubMed: 15961532]  [MGI Ref ID J:101244]

McCullough LD; Zeng Z; Blizzard KK; Debchoudhury I; Hurn PD. 2005. Ischemic nitric oxide and poly (ADP-ribose) polymerase-1 in cerebral ischemia: male toxicity, female protection. J Cereb Blood Flow Metab 25(4):502-12. [PubMed: 15689952]  [MGI Ref ID J:112492]

McKinnon RL; Lidington D; Bolon M; Ouellette Y; Kidder GM; Tyml K. 2006. Reduced arteriolar conducted vasoconstriction in septic mouse cremaster muscle is mediated by nNOS-derived NO. Cardiovasc Res 69(1):236-44. [PubMed: 16226732]  [MGI Ref ID J:112791]

Moayeri M; Crown D; Dorward DW; Gardner D; Ward JM; Li Y; Cui X; Eichacker P; Leppla SH. 2009. The heart is an early target of anthrax lethal toxin in mice: a protective role for neuronal nitric oxide synthase (nNOS). PLoS Pathog 5(5):e1000456. [PubMed: 19478875]  [MGI Ref ID J:162184]

Moens AL; Leyton-Mange JS; Niu X; Yang R; Cingolani O; Arkenbout EK; Champion HC; Bedja D; Gabrielson KL; Chen J; Xia Y; Hale AB; Channon KM; Halushka MK; Barker N; Wuyts FL; Kaminski PM; Wolin MS; Kass DA; Barouch LA. 2009. Adverse ventricular remodeling and exacerbated NOS uncoupling from pressure-overload in mice lacking the beta3-adrenoreceptor. J Mol Cell Cardiol 47(5):576-85. [PubMed: 19766235]  [MGI Ref ID J:155006]

Moraes JC; Amaral ME; Picardi PK; Calegari VC; Romanatto T; Bermudez-Echeverry M; Chiavegatto S; Saad MJ; Velloso LA. 2006. Inducible-NOS but not neuronal-NOS participate in the acute effect of TNF-alpha on hypothalamic insulin-dependent inhibition of food intake. FEBS Lett 580(19):4625-31. [PubMed: 16876161]  [MGI Ref ID J:112153]

Morairty SR; Dittrich L; Pasumarthi RK; Valladao D; Heiss JE; Gerashchenko D; Kilduff TS. 2013. A role for cortical nNOS/NK1 neurons in coupling homeostatic sleep drive to EEG slow wave activity. Proc Natl Acad Sci U S A 110(50):20272-7. [PubMed: 24191004]  [MGI Ref ID J:205036]

Morishita T; Tsutsui M; Shimokawa H; Horiuchi M; Tanimoto A; Suda O; Tasaki H; Huang PL; Sasaguri Y; Yanagihara N; Nakashima Y. 2002. Vasculoprotective roles of neuronal nitric oxide synthase. FASEB J 16(14):1994-6. [PubMed: 12397095]  [MGI Ref ID J:120009]

Morishita T; Tsutsui M; Shimokawa H; Sabanai K; Tasaki H; Suda O; Nakata S; Tanimoto A; Wang KY; Ueta Y; Sasaguri Y; Nakashima Y; Yanagihara N. 2005. Nephrogenic diabetes insipidus in mice lacking all nitric oxide synthase isoforms. Proc Natl Acad Sci U S A 102(30):10616-21. [PubMed: 16024729]  [MGI Ref ID J:100308]

Mustafa AK; Kumar M; Selvakumar B; Ho GP; Ehmsen JT; Barrow RK; Amzel LM; Snyder SH. 2007. Nitric oxide S-nitrosylates serine racemase, mediating feedback inhibition of D-serine formation. Proc Natl Acad Sci U S A 104(8):2950-5. [PubMed: 17293453]  [MGI Ref ID J:125938]

Naghashpour M; Dahl G. 2000. Relaxation of myometrium by calcitonin gene-related peptide is independent of nitric oxide synthase activity in mouse uterus. Biol Reprod 63(5):1421-7. [PubMed: 11058547]  [MGI Ref ID J:108667]

Nakata S; Tsutsui M; Shimokawa H; Suda O; Morishita T; Shibata K; Yatera Y; Sabanai K; Tanimoto A; Nagasaki M; Tasaki H; Sasaguri Y; Nakashima Y; Otsuji Y; Yanagihara N. 2008. Spontaneous myocardial infarction in mice lacking all nitric oxide synthase isoforms. Circulation 117(17):2211-23. [PubMed: 18413498]  [MGI Ref ID J:155090]

Nangle MR; Cotter MA; Cameron NE. 2003. An in vitro study of corpus cavernosum and aorta from mice lacking the inducible nitric oxide synthase gene. Nitric Oxide 9(4):194-200. [PubMed: 14996426]  [MGI Ref ID J:119059]

Nelson RJ; Demas GE; Huang PL; Fishman MC; Dawson VL; Dawson TM; Snyder SH. 1995. Behavioural abnormalities in male mice lacking neuronal nitric oxide synthase [see comments] Nature 378(6555):383-6. [PubMed: 7477374]  [MGI Ref ID J:29970]

Noh HS; Kim DW; Cho GJ; Choi WS; Kang SS. 2006. Increased nitric oxide caused by the ketogenic diet reduces the onset time of kainic acid-induced seizures in ICR mice. Brain Res 1075(1):193-200. [PubMed: 16460714]  [MGI Ref ID J:107300]

Nott A; Nitarska J; Veenvliet JV; Schacke S; Derijck AA; Sirko P; Muchardt C; Pasterkamp RJ; Smidt MP; Riccio A. 2013. S-nitrosylation of HDAC2 regulates the expression of the chromatin-remodeling factor Brm during radial neuron migration. Proc Natl Acad Sci U S A 110(8):3113-8. [PubMed: 23359715]  [MGI Ref ID J:194321]

Nott A; Watson PM; Robinson JD; Crepaldi L; Riccio A. 2008. S-Nitrosylation of histone deacetylase 2 induces chromatin remodelling in neurons. Nature 455(7211):411-5. [PubMed: 18754010]  [MGI Ref ID J:140120]

O'Dell TJ; Huang PL; Dawson TM; Dinerman JL; Snyder SH; Kandel ER; Fishman MC. 1994. Endothelial NOS and the blockade of LTP by NOS inhibitors in mice lacking neuronal NOS. Science 265(5171):542-6. [PubMed: 7518615]  [MGI Ref ID J:19282]

O'Riordan E; Mendelev N; Patschan S; Patschan D; Eskander J; Cohen-Gould L; Chander P; Goligorsky MS. 2007. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292(1):H285-94. [PubMed: 16963618]  [MGI Ref ID J:119956]

Paliege A; Mizel D; Medina C; Pasumarthy A; Huang YG; Bachmann S; Briggs JP; Schnermann JB; Yang T. 2004. Inhibition of nNOS expression in the macula densa by COX-2-derived prostaglandin E(2). Am J Physiol Renal Physiol 287(1):F152-9. [PubMed: 15010356]  [MGI Ref ID J:113758]

Parathath SR; Gravanis I; Tsirka SE. 2007. Nitric oxide synthase isoforms undertake unique roles during excitotoxicity. Stroke 38(6):1938-45. [PubMed: 17446423]  [MGI Ref ID J:150557]

Patzak A; Steege A; Lai EY; Brinkmann JO; Kupsch E; Spielmann N; Gericke A; Skalweit A; Stegbauer J; Persson PB; Seeliger E. 2008. Angiotensin II response in afferent arterioles of mice lacking either the endothelial or neuronal isoform of nitric oxide synthase. Am J Physiol Regul Integr Comp Physiol 294(2):R429-37. [PubMed: 17959704]  [MGI Ref ID J:148658]

Percival JM; Anderson KN; Gregorevic P; Chamberlain JS; Froehner SC. 2008. Functional deficits in nNOSmu-deficient skeletal muscle: myopathy in nNOS knockout mice. PLoS ONE 3(10):e3387. [PubMed: 18852886]  [MGI Ref ID J:144588]

Percival JM; Anderson KN; Huang P; Adams ME; Froehner SC. 2010. Golgi and sarcolemmal neuronal NOS differentially regulate contraction-induced fatigue and vasoconstriction in exercising mouse skeletal muscle. J Clin Invest 120(3):816-26. [PubMed: 20124730]  [MGI Ref ID J:158716]

Phillips KG; Hardingham NR; Fox K. 2008. Postsynaptic action potentials are required for nitric-oxide-dependent long-term potentiation in CA1 neurons of adult GluR1 knock-out and wild-type mice. J Neurosci 28(52):14031-41. [PubMed: 19109486]  [MGI Ref ID J:143884]

Phillipson M; Henriksnas J; Holstad M; Sandler S; Holm L. 2003. Inducible nitric oxide synthase is involved in acid-induced gastric hyperemia in rats and mice. Am J Physiol Gastrointest Liver Physiol 285(1):G154-62. [PubMed: 12646421]  [MGI Ref ID J:84264]

Phillipson M; Johansson ME; Henriksnas J; Petersson J; Gendler SJ; Sandler S; Persson AE; Hansson GC; Holm L. 2008. The gastric mucus layers: constituents and regulation of accumulation. Am J Physiol Gastrointest Liver Physiol 295(4):G806-12. [PubMed: 18719000]  [MGI Ref ID J:142280]

Putzke J; Seidel B; Huang PL; Wolf G. 2000. Differential expression of alternatively spliced isoforms of neuronal nitric oxide synthase (nNOS) and N-methyl-D-aspartate receptors (NMDAR) in knockout mice deficient in nNOS alpha (nNOS alpha(Delta/Delta) mice). Brain Res Mol Brain Res 85(1-2):13-23. [PubMed: 11146102]  [MGI Ref ID J:66968]

Rattan S; Regan RF; Patel CA; De Godoy MA. 2005. Nitric oxide not carbon monoxide mediates nonadrenergic noncholinergic relaxation in the murine internal anal sphincter. Gastroenterology 129(6):1954-66. [PubMed: 16344064]  [MGI Ref ID J:124936]

Riccio A; Alvania RS; Lonze BE; Ramanan N; Kim T; Huang Y; Dawson TM; Snyder SH; Ginty DD. 2006. A nitric oxide signaling pathway controls CREB-mediated gene expression in neurons. Mol Cell 21(2):283-94. [PubMed: 16427017]  [MGI Ref ID J:166020]

Rothe F; Huang PL; Wolf G. 1999. Ultrastructural localization of neuronal nitric oxide synthase in the laterodorsal tegmental nucleus of wild-type and knockout mice. Neuroscience 94(1):193-201. [PubMed: 10613509]  [MGI Ref ID J:59780]

Rudkowski JC; Barreiro E; Harfouche R; Goldberg P; Kishta O; D'Orleans-Juste P; Labonte J; Lesur O; Hussain SN. 2004. Roles of iNOS and nNOS in sepsis-induced pulmonary apoptosis. Am J Physiol Lung Cell Mol Physiol 286(4):L793-800. [PubMed: 14660484]  [MGI Ref ID J:108149]

Sabanai K; Tsutsui M; Sakai A; Hirasawa H; Tanaka S; Nakamura E; Tanimoto A; Sasaguri Y; Ito M; Shimokawa H; Nakamura T; Yanagihara N. 2008. Genetic disruption of all NO synthase isoforms enhances BMD and bone turnover in mice in vivo: involvement of the renin-angiotensin system. J Bone Miner Res 23(5):633-43. [PubMed: 18433298]  [MGI Ref ID J:150159]

Salchner P; Lubec G; Engelmann M; Orlando GF; Wolf G; Sartori SB; Hoeger H; Singewald N. 2004. Genetic functional inactivation of neuronal nitric oxide synthase affects stress-related Fos expression in specific brain regions. Cell Mol Life Sci 61(12):1498-506. [PubMed: 15197473]  [MGI Ref ID J:115481]

Sampei K; Mandir AS; Asano Y; Wong PC; Traystman RJ; Dawson VL; Dawson TM; Hurn PD. 2000. Stroke outcome in double-mutant antioxidant transgenic mice. Stroke 31(11):2685-91. [PubMed: 11062295]  [MGI Ref ID J:128835]

Saraiva RM; Minhas KM; Raju SV; Barouch LA; Pitz E; Schuleri KH; Vandegaer K; Li D; Hare JM. 2005. Deficiency of neuronal nitric oxide synthase increases mortality and cardiac remodeling after myocardial infarction: role of nitroso-redox equilibrium. Circulation 112(22):3415-22. [PubMed: 16301341]  [MGI Ref ID J:116907]

Sartoretto JL; Jin BY; Bauer M; Gertler FB; Liao R; Michel T. 2009. Regulation of VASP phosphorylation in cardiac myocytes: differential regulation by cyclic nucleotides and modulation of protein expression in diabetic and hypertrophic heart. Am J Physiol Heart Circ Physiol 297(5):H1697-710. [PubMed: 19734360]  [MGI Ref ID J:154317]

Sartoretto JL; Kalwa H; Pluth MD; Lippard SJ; Michel T. 2011. Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis. Proc Natl Acad Sci U S A 108(38):15792-7. [PubMed: 21896719]  [MGI Ref ID J:176589]

Scheiner CA; Cork RJ; Mize RR. 1999. Failure to disrupt development of cholinergic fiber patches in the superior colliculus in nitric oxide synthase deficient mice. Brain Res Dev Brain Res 118(1-2):217-20. [PubMed: 10611522]  [MGI Ref ID J:59193]

Schild L; Dombrowski F; Lendeckel U; Schulz C; Gardemann A; Keilhoff G. 2008. Impairment of endothelial nitric oxide synthase causes abnormal fat and glycogen deposition in liver. Biochim Biophys Acta 1782(3):180-7. [PubMed: 18206129]  [MGI Ref ID J:133437]

Schild L; Jaroscakova I; Lendeckel U; Wolf G; Keilhoff G. 2006. Neuronal nitric oxide synthase controls enzyme activity pattern of mitochondria and lipid metabolism. FASEB J 20(1):145-7. [PubMed: 16246868]  [MGI Ref ID J:104558]

Schnermann J. 1999. Micropuncture analysis of tubuloglomerular feedback regulation in transgenic mice. J Am Soc Nephrol 10(12):2614-9. [PubMed: 10589702]  [MGI Ref ID J:59838]

Schuh K; Quaschning T; Knauer S; Hu K; Kocak S; Roethlein N; Neyses L. 2003. Regulation of vascular tone in animals overexpressing the sarcolemmal calcium pump. J Biol Chem 278(42):41246-52. [PubMed: 12900399]  [MGI Ref ID J:119413]

Schulz JB; Huang PL; Matthews RT; Passov D; Fishman MC; Beal MF. 1996. Striatal malonate lesions are attenuated in neuronal nitric oxide synthase knockout mice. J Neurochem 67(1):430-3. [PubMed: 8667023]  [MGI Ref ID J:111644]

Sears CE; Bryant SM; Ashley EA; Lygate CA; Rakovic S; Wallis HL; Neubauer S; Terrar DA; Casadei B. 2003. Cardiac neuronal nitric oxide synthase isoform regulates myocardial contraction and calcium handling. Circ Res 92(5):e52-9. [PubMed: 12623875]  [MGI Ref ID J:109339]

Shankar RR; Wu Y; Shen HQ; Zhu JS; Baron AD. 2000. Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance. Diabetes 49(5):684-7. [PubMed: 10905473]  [MGI Ref ID J:62229]

Shesely EG; Gilbert C; Granderson G; Carretero CD; Carretero OA; Beierwaltes WH. 2001. Nitric oxide synthase gene knockout mice do not become hypertensive during pregnancy. Am J Obstet Gynecol 185(5):1198-203. [PubMed: 11717657]  [MGI Ref ID J:117212]

Shiao T; Fond A; Deng B; Wehling-Henricks M; Adams ME; Froehner SC; Tidball JG. 2004. Defects in neuromuscular junction structure in dystrophic muscle are corrected by expression of a NOS transgene in dystrophin-deficient muscles, but not in muscles lacking {alpha}- and {beta}1-syntrophins. Hum Mol Genet 13(17):1873-1884. [PubMed: 15238508]  [MGI Ref ID J:92094]

Shimazu T; Otani H; Yoshioka K; Fujita M; Okazaki T; Iwasaka T. 2011. Sepiapterin enhances angiogenesis and functional recovery in mice after myocardial infarction. Am J Physiol Heart Circ Physiol 301(5):H2061-72. [PubMed: 21890687]  [MGI Ref ID J:178332]

Shimizu-Sasamata M; Bosque-Hamilton P; Huang PL; Moskowitz MA; Lo EH. 1998. Attenuated neurotransmitter release and spreading depression-like depolarizations after focal ischemia in mutant mice with disrupted type I nitric oxide synthase gene. J Neurosci 18(22):9564-71. [PubMed: 9801393]  [MGI Ref ID J:50898]

Shutoh F; Ohki M; Kitazawa H; Itohara S; Nagao S. 2006. Memory trace of motor learning shifts transsynaptically from cerebellar cortex to nuclei for consolidation. Neuroscience 139(2):767-777. [PubMed: 16458438]  [MGI Ref ID J:107819]

Sivarao DV; Mashimo H; Goyal RK. 2008. Pyloric sphincter dysfunction in nNOS-/- and W/Wv mutant mice: animal models of gastroparesis and duodenogastric reflux. Gastroenterology 135(4):1258-66. [PubMed: 18640116]  [MGI Ref ID J:142004]

Sivarao DV; Mashimo HL; Thatte HS; Goyal RK. 2001. Lower esophageal sphincter is achalasic in nNOS(-/-) and hypotensive in W/W(v) mutant mice. Gastroenterology 121(1):34-42. [PubMed: 11438492]  [MGI Ref ID J:70182]

Son H; Hawkins RD; Martin K; Kiebler M; Huang PL; Fishman MC; Kandel ER. 1996. Long-term potentiation is reduced in mice that are doubly mutant in endothelial and neuronal nitric oxide synthase. Cell 87(6):1015-23. [PubMed: 8978606]  [MGI Ref ID J:37956]

Spanagel R; Siegmund S; Cowen M; Schroff KC; Schumann G; Fiserova M; Sillaber I; Wellek S; Singer M; Putzke J. 2002. The neuronal nitric oxide synthase gene is critically involved in neurobehavioral effects of alcohol. J Neurosci 22(19):8676-83. [PubMed: 12351742]  [MGI Ref ID J:79215]

Steed MM; Tyagi N; Sen U; Schuschke DA; Joshua IG; Tyagi SC. 2010. Functional consequences of the collagen/elastin switch in vascular remodeling in hyperhomocysteinemic wild-type, eNOS-/-, and iNOS-/- mice. Am J Physiol Lung Cell Mol Physiol 299(3):L301-11. [PubMed: 20581102]  [MGI Ref ID J:164613]

Stegbauer J; Kuczka Y; Vonend O; Quack I; Sellin L; Patzak A; Steege A; Langnaese K; Rump LC. 2008. Endothelial nitric oxide synthase is predominantly involved in angiotensin II modulation of renal vascular resistance and norepinephrine release. Am J Physiol Regul Integr Comp Physiol 294(2):R421-8. [PubMed: 18046021]  [MGI Ref ID J:141547]

Steudel W; Kirmse M; Weimann J; Ullrich R; Hromi J; Zapol WM. 2000. Exhaled nitric oxide production by nitric oxide synthase-deficient mice. Am J Respir Crit Care Med 162(4 Pt 1):1262-7. [PubMed: 11029328]  [MGI Ref ID J:103210]

Sumeray MS; Rees DD; Yellon DM. 2000. Infarct size and nitric oxide synthase in murine myocardium J Mol Cell Cardiol 32(1):35-42. [PubMed: 10652188]  [MGI Ref ID J:60525]

Sun J; Picht E; Ginsburg KS; Bers DM; Steenbergen C; Murphy E. 2006. Hypercontractile female hearts exhibit increased S-nitrosylation of the L-type Ca2+ channel alpha1 subunit and reduced ischemia/reperfusion injury. Circ Res 98(3):403-11. [PubMed: 16397145]  [MGI Ref ID J:118890]

Sun Y; Jin K; Childs JT; Xie L; Mao XO; Greenberg DA. 2005. Neuronal nitric oxide synthase and ischemia-induced neurogenesis. J Cereb Blood Flow Metab 25(4):485-92. [PubMed: 15689958]  [MGI Ref ID J:112491]

Suzuki N; Motohashi N; Uezumi A; Fukada S; Yoshimura T; Itoyama Y; Aoki M; Miyagoe-Suzuki Y; Takeda S. 2007. NO production results in suspension-induced muscle atrophy through dislocation of neuronal NOS. J Clin Invest 117(9):2468-76. [PubMed: 17786240]  [MGI Ref ID J:127415]

Szabadits E; Cserep C; Ludanyi A; Katona I; Gracia-Llanes J; Freund TF; Nyiri G. 2007. Hippocampal GABAergic synapses possess the molecular machinery for retrograde nitric oxide signaling. J Neurosci 27(30):8101-11. [PubMed: 17652601]  [MGI Ref ID J:123246]

Takaki A; Morikawa K; Tsutsui M; Murayama Y; Tekes E; Yamagishi H; Ohashi J; Yada T; Yanagihara N; Shimokawa H. 2008. Crucial role of nitric oxide synthases system in endothelium-dependent hyperpolarization in mice. J Exp Med 205(9):2053-63. [PubMed: 18695006]  [MGI Ref ID J:140478]

Tao F; Tao YX; Zhao C; Dore S; Liaw WJ; Raja SN; Johns RA. 2004. Differential roles of neuronal and endothelial nitric oxide synthases during carrageenan-induced inflammatory hyperalgesia. Neuroscience 128(2):421-30. [PubMed: 15350652]  [MGI Ref ID J:93612]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Terauchi A; Kobayashi D; Mashimo H. 2005. Distinct roles of nitric oxide synthases and interstitial cells of Cajal in rectoanal relaxation. Am J Physiol Gastrointest Liver Physiol 289(2):G291-9. [PubMed: 15845873]  [MGI Ref ID J:100350]

Tranguch S; Huet-Hudson Y. 2003. Decreased viability of nitric oxide synthase double knockout mice. Mol Reprod Dev 65(2):175-9. [PubMed: 12704728]  [MGI Ref ID J:83112]

Tsui AK; Marsden PA; Mazer CD; Adamson SL; Henkelman RM; Ho JJ; Wilson DF; Heximer SP; Connelly KA; Bolz SS; Lidington D; El-Beheiry MH; Dattani ND; Chen KM; Hare GM. 2011. Priming of hypoxia-inducible factor by neuronal nitric oxide synthase is essential for adaptive responses to severe anemia. Proc Natl Acad Sci U S A 108(42):17544-9. [PubMed: 21976486]  [MGI Ref ID J:177441]

Vallance BA; Dijkstra G; Qiu B; van der Waaij LA; van Goor H; Jansen PL; Mashimo H; Collins SM. 2004. Relative contributions of NOS isoforms during experimental colitis: endothelial-derived NOS maintains mucosal integrity. Am J Physiol Gastrointest Liver Physiol 287(4):G865-74. [PubMed: 15217783]  [MGI Ref ID J:96074]

Vandsburger MH; French BA; Kramer CM; Zhong X; Epstein FH. 2012. Displacement-encoded and manganese-enhanced cardiac MRI reveal that nNOS, not eNOS, plays a dominant role in modulating contraction and calcium influx in the mammalian heart. Am J Physiol Heart Circ Physiol 302(2):H412-9. [PubMed: 22058155]  [MGI Ref ID J:181655]

Wagner C; Godecke A; Ford M; Schnermann J; Schrader J; Kurtz A. 2000. Regulation of renin gene expression in kidneys of eNOS- and nNOS-deficient mice Pflugers Arch 439(5):567-72. [PubMed: 10764216]  [MGI Ref ID J:62375]

Walton JC; Selvakumar B; Weil ZM; Snyder SH; Nelson RJ. 2013. Neuronal nitric oxide synthase and NADPH oxidase interact to affect cognitive, affective, and social behaviors in mice. Behav Brain Res 256:320-7. [PubMed: 23948215]  [MGI Ref ID J:202345]

Wang H; Kohr MJ; Traynham CJ; Wheeler DG; Janssen PM; Ziolo MT. 2008. Neuronal nitric oxide synthase signaling within cardiac myocytes targets phospholamban. Am J Physiol Cell Physiol 294(6):C1566-75. [PubMed: 18400986]  [MGI Ref ID J:136639]

Wang H; Viatchenko-Karpinski S; Sun J; Gyorke I; Benkusky NA; Kohr MJ; Valdivia HH; Murphy E; Gyorke S; Ziolo MT. 2010. Regulation of myocyte contraction via neuronal nitric oxide synthase: role of ryanodine receptor S-nitrosylation. J Physiol 588(Pt 15):2905-17. [PubMed: 20530114]  [MGI Ref ID J:176763]

Wang T; Inglis FM; Kalb RG. 2000. Defective fluid and HCO(3)(-) absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice. Am J Physiol Renal Physiol 279(3):F518-24. [PubMed: 10966931]  [MGI Ref ID J:64896]

Watkins CC; Boehning D; Kaplin AI; Rao M; Ferris CD; Snyder SH. 2004. Carbon monoxide mediates vasoactive intestinal polypeptide-associated nonadrenergic/noncholinergic neurotransmission. Proc Natl Acad Sci U S A 101(8):2631-5. [PubMed: 14983060]  [MGI Ref ID J:88640]

Watkins CC; Sawa A; Jaffrey S; Blackshaw S; Barrow RK; Snyder SH; Ferris CD. 2000. Insulin restores neuronal nitric oxide synthase expression and function that is lost in diabetic gastropathy. J Clin Invest 106(3):373-84. [PubMed: 10930440]  [MGI Ref ID J:63749]

Wehling-Henricks M; Oltmann M; Rinaldi C; Myung KH; Tidball JG. 2009. Loss of positive allosteric interactions between neuronal nitric oxide synthase and phosphofructokinase contributes to defects in glycolysis and increased fatigability in muscular dystrophy. Hum Mol Genet 18(18):3439-51. [PubMed: 19542095]  [MGI Ref ID J:151716]

Weitzdoerfer R; Hoeger H; Engidawork E; Engelmann M; Singewald N; Lubec G; Lubec B. 2004. Neuronal nitric oxide synthase knock-out mice show impaired cognitive performance. Nitric Oxide 10(3):130-40. [PubMed: 15158692]  [MGI Ref ID J:101815]

Workman JL; Trainor BC; Finy MS; Nelson RJ. 2008. Inhibition of neuronal nitric oxide reduces anxiety-like responses to pair housing. Behav Brain Res 187(1):109-15. [PubMed: 17928072]  [MGI Ref ID J:145099]

Wu HH; Cork RJ; Huang PL; Shuman DL; Mize RR. 2000. Refinement of the ipsilateral retinocollicular projection is disrupted in double endothelial and neuronal nitric oxide synthase gene knockout mice. Brain Res Dev Brain Res 120(1):105-11. [PubMed: 10727738]  [MGI Ref ID J:61144]

Wu HH; Cork RJ; Mize RR. 2000. Normal development of the ipsilateral retinocollicular pathway and its disruption in double endothelial and neuronal nitric oxide synthase gene knockout mice J Comp Neurol 426(4):651-65. [PubMed: 11027405]  [MGI Ref ID J:64999]

Xu L; Okuda-Ashitaka E; Matsumura S; Mabuchi T; Okamoto S; Sakimura K; Mishina M; Ito S. 2007. Signal pathways coupled to activation of neuronal nitric oxide synthase in the spinal cord by nociceptin/orphanin FQ. Neuropharmacology 52(5):1318-25. [PubMed: 17350656]  [MGI Ref ID J:122321]

Xu R; Serritella AV; Sen T; Farook JM; Sedlak TW; Baraban J; Snyder SH; Sen N. 2013. Behavioral effects of cocaine mediated by nitric oxide-GAPDH transcriptional signaling. Neuron 78(4):623-30. [PubMed: 23719162]  [MGI Ref ID J:201545]

Xue L; Farrugia G; Miller SM; Ferris CD; Snyder SH; Szurszewski JH. 2000. Carbon monoxide and nitric oxide as coneurotransmitters in the enteric nervous system: evidence from genomic deletion of biosynthetic enzymes. Proc Natl Acad Sci U S A 97(4):1851-5. [PubMed: 10677545]  [MGI Ref ID J:89581]

Yang G; Zhang Y; Ross ME; Iadecola C. 2003. Attenuation of activity-induced increases in cerebellar blood flow in mice lacking neuronal nitric oxide synthase. Am J Physiol Heart Circ Physiol 285(1):H298-304. [PubMed: 12623792]  [MGI Ref ID J:84255]

Yang JZ; Ajonuma LC; Rowlands DK; Tsang LL; Ho LS; Lam SY; Chen WY; Zhou CX; Chung YW; Cho CY; Tse JY; James AE; Chan HC. 2005. The role of inducible nitric oxide synthase in gamete interaction and fertilization: a comparative study on knockout mice of three NOS isoforms. Cell Biol Int 29(9):785-91. [PubMed: 16087361]  [MGI Ref ID J:112824]

Yatera Y; Shibata K; Furuno Y; Sabanai K; Morisada N; Nakata S; Morishita T; Toyohira Y; Wang KY; Tanimoto A; Sasaguri Y; Tasaki H; Nakashima Y; Shimokawa H; Yanagihara N; Otsuji Y; Tsutsui M. 2010. Severe dyslipidaemia, atherosclerosis, and sudden cardiac death in mice lacking all NO synthases fed a high-fat diet. Cardiovasc Res 87(4):675-82. [PubMed: 20304785]  [MGI Ref ID J:176098]

Yemisci M; Gursoy-Ozdemir Y; Vural A; Can A; Topalkara K; Dalkara T. 2009. Pericyte contraction induced by oxidative-nitrative stress impairs capillary reflow despite successful opening of an occluded cerebral artery. Nat Med 15(9):1031-7. [PubMed: 19718040]  [MGI Ref ID J:154320]

Zaharchuk G; Hara H; Huang PL; Fishman MC; Moskowitz MA; Jenkins BG ; Rosen BR. 1997. Neuronal nitric oxide synthase mutant mice show smaller infarcts and attenuated apparent diffusion coefficient changes in the peri-infarct zone during focal cerebral ischemia. Magn Reson Med 37(2):170-5. [PubMed: 9001139]  [MGI Ref ID J:40122]

Zakhary R; Poss KD; Jaffrey SR; Ferris CD; Tonegawa S; Snyder SH. 1997. Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide. Proc Natl Acad Sci U S A 94(26):14848-53. [PubMed: 9405702]  [MGI Ref ID J:89562]

Zhang J; Huang XY; Ye ML; Luo CX; Wu HY; Hu Y; Zhou QG; Wu DL; Zhu LJ; Zhu DY. 2010. Neuronal nitric oxide synthase alteration accounts for the role of 5-HT1A receptor in modulating anxiety-related behaviors. J Neurosci 30(7):2433-41. [PubMed: 20164327]  [MGI Ref ID J:157840]

Zhang YH; Zhang MH; Sears CE; Emanuel K; Redwood C; El-Armouche A; Kranias EG; Casadei B. 2008. Reduced phospholamban phosphorylation is associated with impaired relaxation in left ventricular myocytes from neuronal NO synthase-deficient mice. Circ Res 102(2):242-9. [PubMed: 18007024]  [MGI Ref ID J:141557]

Zhou L; Li F; Xu HB; Luo CX; Wu HY; Zhu MM; Lu W; Ji X; Zhou QG; Zhu DY. 2010. Treatment of cerebral ischemia by disrupting ischemia-induced interaction of nNOS with PSD-95. Nat Med 16(12):1439-43. [PubMed: 21102461]  [MGI Ref ID J:167517]

Zhu H; Bhattacharyya BJ; Lin H; Gomez CM. 2013. Skeletal muscle calpain acts through nitric oxide and neural miRNAs to regulate acetylcholine release in motor nerve terminals. J Neurosci 33(17):7308-24. [PubMed: 23616539]  [MGI Ref ID J:196946]

Zhu XJ; Hua Y; Jiang J; Zhou QG; Luo CX; Han X; Lu YM; Zhu DY. 2006. Neuronal nitric oxide synthase-derived nitric oxide inhibits neurogenesis in the adult dentate gyrus by down-regulating cyclic AMP response element binding protein phosphorylation. Neuroscience 141(2):827-36. [PubMed: 16735094]  [MGI Ref ID J:111759]

Zhu Y; Ohlemiller KK; McMahan BK; Park TS; Gidday JM. 2006. Constitutive nitric oxide synthase activity is required to trigger ischemic tolerance in mouse retina. Exp Eye Res 82(1):153-63. [PubMed: 16045907]  [MGI Ref ID J:106447]

Zhuang W; Eby JC; Cheong M; Mohapatra PK; Bredt DS; Disatnik MH; Rando TA. 2001. The susceptibility of muscle cells to oxidative stress is independent of nitric oxide synthase expression. Muscle Nerve 24(4):502-11. [PubMed: 11268022]  [MGI Ref ID J:116198]

Zoubovsky SP; Pogorelov VM; Taniguchi Y; Kim SH; Yoon P; Nwulia E; Sawa A; Pletnikov MV; Kamiya A. 2011. Working memory deficits in neuronal nitric oxide synthase knockout mice: Potential impairments in prefrontal cortex mediated cognitive function. Biochem Biophys Res Commun 408(4):707-12. [PubMed: 21539806]  [MGI Ref ID J:172382]

de Jonge WJ; Hallemeesch MM; Kwikkers KL; Ruijter JM; de Gier-de Vries C; van Roon MA; Meijer AJ; Marescau B; de Deyn PP; Deutz NE; Lamers WH. 2002. Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development. Am J Clin Nutr 76(1):128-40. [PubMed: 12081826]  [MGI Ref ID J:80556]

de Jonge WJ; Kwikkers KL; te Velde AA; van Deventer SJ; Nolte MA; Mebius RE; Ruijter JM; Lamers MC; Lamers WH. 2002. Arginine deficiency affects early B cell maturation and lymphoid organ development in transgenic mice. J Clin Invest 110(10):1539-48. [PubMed: 12438451]  [MGI Ref ID J:80204]

de Vasconcelos AP; Bouilleret V; Riban V; Wasterlain C; Nehlig A. 2005. Role of nitric oxide in cerebral blood flow changes during kainate seizures in mice: genetic and pharmacological approaches. Neurobiol Dis 18(2):270-81. [PubMed: 15686955]  [MGI Ref ID J:124446]

van't Hof RJ; Macphee J; Libouban H; Helfrich MH; Ralston SH. 2004. Regulation of bone mass and bone turnover by neuronal nitric oxide synthase. Endocrinology 145(11):5068-74. [PubMed: 15297441]  [MGI Ref ID J:105612]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Mating SystemHomozygote x Heterozygote         (Female x Male)   04-APR-13
Heterozygote x Homozygote         (Female x Male)   04-APR-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Nos1tm1Plh  
$232.00Female or MaleHomozygous for Nos1tm1Plh  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Nos1tm1Plh x Homozygous for Nos1tm1Plh  
$464.00Homozygous for Nos1tm1Plh x Heterozygous for Nos1tm1Plh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Nos1tm1Plh  
$301.60Female or MaleHomozygous for Nos1tm1Plh  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Nos1tm1Plh x Homozygous for Nos1tm1Plh  
$603.20Homozygous for Nos1tm1Plh x Heterozygous for Nos1tm1Plh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Important Note

The mice of this strain have been backcrossed more than 10 backcrosses to C57BL/6J, making this strain more effective for studies of the Nos1 KO than the incipient stock (#002633).

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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