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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S7 via AB1 ES cell line (+Hprt-bm2) Generation N?+5F15N1P
Generation DefinitionsDonating Investigator The Jackson Laboratory, Appearance
black
Related Genotype: a/aDescription
Mice homozygous for the Lrpap1tm1Her targeted mutation (commonly referred to as RAP) are viable and fertile. They have a functional deficiency of the low density lipoprotein receptor-related protein (LRP) with reduced amounts of LRP seen in both the liver and the brain of homozygotes.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Lrpap1tm1Her allele
002465 B6;129S-Lrpap1tm1Her Ldlrtm1Her/J 002464 B6;129S7-Lrpap1tm1Her/J View Strains carrying Lrpap1tm1Her (2 strains)
Phenotype
Phenotype Information
assigned by genotype
Lrpap1tm1Her/Lrpap1tm1Her
B6.129S7-Lrpap1tm1Her
- renal/urinary system phenotype
- abnormal kidney physiology
- endocytotic machinery in the proximal tubule is disturbed athough the ability to adjust to dietary phosphate levels is unaffected (MGI Ref ID J:106203)
- endocrine/exocrine gland phenotype
- abnormal thyroid gland morphology
- slight thyrocyte and nuclear hypertrophy but no goiter (MGI Ref ID J:107358)
- abnormal thyroid physiology
- slightly reduced thyroglobulin in thyroid follicles but intracellular levels and levels on the apical membrane of thyrocytes are increased (MGI Ref ID J:107358)
- T4 levels in thyroglobulin are normal or slightly elevated (MGI Ref ID J:107358)
- free T4 levels unchanged (MGI Ref ID J:107358)
- TSH levels are elevated but not to the point of statistical significance (MGI Ref ID J:107358)
- nervous system phenotype
- abnormal brain interneuron morphology
- behavior/neurological phenotype
- abnormal spatial learning
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Lrpap1tm1Her/Lrpap1tm1Her
involves: 129S7/SvEvBrd * C57BL/6J
- homeostasis/metabolism phenotype
- abnormal circulating protein level
- other phenotype
- other aberrant phenotype
- reduces expression of lipoprotein receptor related protein by 75% but has no effect on the LDL receptor (MGI Ref ID J:43736)
This mouse can be used to support research in many areas including:Lrpap1tm1Her related
Cardiovascular Research
Other
altered lipoprotein profile
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Lrpap1tm1Her | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joachim Herz | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | RAP-; | ||
| Mutation Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | 129S7/SvEvBrd | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Lrpap1, low density lipoprotein receptor-related protein associated protein 1 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | A2MRAP; A2RAP; AA617339; AI790446; AU042172; C77774; HBP44; MRAP; RAP; expressed sequence AA617339; expressed sequence AI790446; expressed sequence AU042172; expressed sequence C77774; | ||
| General Note | ES cell line = JH1 or AB1 (129S7/SvEvBrd-Hprt+). | ||
| Molecular Note | A neomycin resistance cassette was inserted into sequences that encode the leader peptide. Immunoblot analysis of liver and brain did not detect the encoded protein in homozygous animals. [MGI Ref ID J:43736] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Lrpaptm1Her, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Willnow TE; Armstrong SA; Hammer RE; Herz J. 1995. Functional expression of low density lipoprotein receptor-related protein is controlled by receptor-associated protein in vivo. Proc Natl Acad Sci U S A 92(10):4537-41. [PubMed: 7538675] [MGI Ref ID J:43736]
Additional References
Willnow TE; Rohlmann A; Horton J; Otani H; Braun JR; Hammer RE; Herz J. 1996. RAP, a specialized chaperone, prevents ligand-induced ER retention and degradation of LDL receptor-related endocytic receptors. EMBO J 15(11):2632-9. [PubMed: 8654360] [MGI Ref ID J:33584]
Lrpap1tm1Her relatedBacic D; Capuano P; Gisler SM; Pribanic S; Christensen EI; Biber J; Loffing J; Kaissling B; Wagner CA; Murer H. 2003. Impaired PTH-induced endocytotic down-regulation of the renal type IIa Na+/Pi-cotransporter in RAP-deficient mice with reduced megalin expression. Pflugers Arch 446(4):475-84. [PubMed: 12748857] [MGI Ref ID J:106203]
Deane R; Sagare A; Hamm K; Parisi M; LaRue B; Guo H; Wu Z; Holtzman DM; Zlokovic BV. 2005. IgG-assisted age-dependent clearance of Alzheimer's amyloid beta peptide by the blood-brain barrier neonatal Fc receptor. J Neurosci 25(50):11495-503. [PubMed: 16354907] [MGI Ref ID J:103998]
Licastro F; Campbell IL; Kincaid C; Veinbergs I; Van Uden E; Rockenstein E; Mallory M; Gilbert JR; Masliah E. 1999. A role for apoE in regulating the levels of alpha-1-antichymotrypsin in the aging mouse brain and in Alzheimer's disease. Am J Pathol 155(3):869-75. [PubMed: 10487844] [MGI Ref ID J:57495]
Lisi S; Botta R; Pinchera A; Collins AB; Refetoff S; Arvan P; Bu G; Grasso L; Marshansky V; Bechoua S; Hurtado-Lorenzo A; Marcocci C; Brown D; McCluskey RT; Marino M. 2006. Defective thyroglobulin storage in LDL receptor-associated protein-deficient mice. Am J Physiol Cell Physiol 290(4):C1160-7. [PubMed: 16306127] [MGI Ref ID J:107358]
Marschang P; Brich J; Weeber EJ; Sweatt JD; Shelton JM; Richardson JA; Hammer RE; Herz J. 2004. Normal development and fertility of knockout mice lacking the tumor suppressor gene LRP1b suggest functional compensation by LRP1. Mol Cell Biol 24(9):3782-93. [PubMed: 15082773] [MGI Ref ID J:90197]
Martins IJ; Hone E; Chi C; Seydel U; Martins RN; Redgrave TG. 2000. Relative roles of LDLr and LRP in the metabolism of chylomicron remnants in genetically manipulated mice. J Lipid Res 41(2):205-13. [PubMed: 10681404] [MGI Ref ID J:120545]
Umans L; Serneels L; Lorent K; Dewachter I; Tesseur I; Moechars D; Van Leuven F. 1999. Lipoprotein receptor-related protein in brain and in cultured neurons of mice deficient in receptor-associated protein and transgenic for apolipoprotein E4 or amyloid precursor protein. Neuroscience 94(1):315-21. [PubMed: 10613521] [MGI Ref ID J:59778]
Van Uden E; Mallory M; Veinbergs I; Alford M; Rockenstein E; Masliah E. 2002. Increased extracellular amyloid deposition and neurodegeneration in human amyloid precursor protein transgenic mice deficient in receptor-associated protein. J Neurosci 22(21):9298-304. [PubMed: 12417655] [MGI Ref ID J:107567]
Van Uden E; Veinbergs I; Mallory M; Orlando R; Masliah E. 1999. A novel role for receptor-associated protein in somatostatin modulation: implications for Alzheimer's disease. Neuroscience 88(3):687-700. [PubMed: 10363810] [MGI Ref ID J:107552]
Veinbergs I; Van Uden E; Mallory M; Alford M; McGiffert C; DeTeresa R; Orlando R; Masliah E. 2001. Role of apolipoprotein E receptors in regulating the differential in vivo neurotrophic effects of apolipoprotein E. Exp Neurol 170(1):15-26. [PubMed: 11421580] [MGI Ref ID J:70482]
Willnow TE; Rohlmann A; Horton J; Otani H; Braun JR; Hammer RE; Herz J. 1996. RAP, a specialized chaperone, prevents ligand-induced ER retention and degradation of LDL receptor-related endocytic receptors. EMBO J 15(11):2632-9. [PubMed: 8654360] [MGI Ref ID J:33584]
Xu G; Karch C; Li N; Lin N; Fromholt D; Gonzales V; Borchelt DR. 2008. Receptor-associated protein (RAP) plays a central role in modulating Abeta deposition in APP/PS1 transgenic mice. PLoS ONE 3(9):e3159. [PubMed: 18776935] [MGI Ref ID J:143942]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When held in a live colony, this strain may be maintained by homozygous siblings matings. Expected coat color from breeding:Black Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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