Strain Name:

B6.129S7-Lrpap1tm1Her/J

Stock Number:

002987

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Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S7 via AB1 ES cell line (+Hprt-bm2)
 
Donating Investigator The Jackson Laboratory,  

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Lrpap1tm1Her targeted mutation (commonly referred to as RAP) are viable and fertile. They have a functional deficiency of the low density lipoprotein receptor-related protein (LRP) with reduced amounts of LRP seen in both the liver and the brain of homozygotes.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Lrpap1tm1Her allele
002465   B6;129S-Lrpap1tm1Her Ldlrtm1Her/J
002464   B6;129S7-Lrpap1tm1Her/J
View Strains carrying   Lrpap1tm1Her     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Myopia 23, Autosomal Recessive; MYP23   (LRPAP1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Lrpap1tm1Her/Lrpap1tm1Her

        B6.129S7-Lrpap1tm1Her
  • renal/urinary system phenotype
  • abnormal kidney physiology
    • endocytotic machinery in the proximal tubule is disturbed athough the ability to adjust to dietary phosphate levels is unaffected   (MGI Ref ID J:106203)
  • endocrine/exocrine gland phenotype
  • abnormal thyroid gland morphology
    • slight thyrocyte and nuclear hypertrophy but no goiter   (MGI Ref ID J:107358)
  • abnormal thyroid physiology
    • slightly reduced thyroglobulin in thyroid follicles but intracellular levels and levels on the apical membrane of thyrocytes are increased   (MGI Ref ID J:107358)
    • T4 levels in thyroglobulin are normal or slightly elevated   (MGI Ref ID J:107358)
    • free T4 levels unchanged   (MGI Ref ID J:107358)
    • TSH levels are elevated but not to the point of statistical significance   (MGI Ref ID J:107358)
  • nervous system phenotype
  • abnormal brain interneuron morphology
    • levels of somatostatin in neurons reduced   (MGI Ref ID J:107552)
    • number of neurons expressing somatostatin is reduced   (MGI Ref ID J:107552)
  • behavior/neurological phenotype
  • abnormal spatial learning
    • poorer performance in a Morris water maze   (MGI Ref ID J:107552)
    • after 6 days of training, swim a greater distance to find target than do controls   (MGI Ref ID J:107552)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Lrpap1tm1Her/Lrpap1tm1Her

        involves: 129S7/SvEvBrd * C57BL/6J
  • homeostasis/metabolism phenotype
  • abnormal circulating protein level
    • alpha2 macroglobulin is removed from the circulation at about 25% of the normal rate   (MGI Ref ID J:43736)
    • no effect is seen on blood lipoproteins regardless of diet   (MGI Ref ID J:43736)
    • homozygotes thrived well and are superficially normal   (MGI Ref ID J:43736)
  • other phenotype
  • other aberrant phenotype
    • reduces expression of lipoprotein receptor related protein by 75% but has no effect on the LDL receptor   (MGI Ref ID J:43736)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Lrpap1tm1Her related

Cardiovascular Research
Other
      altered lipoprotein profile

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lrpap1tm1Her
Allele Name targeted mutation 1, Joachim Herz
Allele Type Targeted (Null/Knockout)
Common Name(s) RAP-;
Mutation Made ByDr. Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd
ES Cell Line NameOther (see notes)
ES Cell Line Strain129
Gene Symbol and Name Lrpap1, low density lipoprotein receptor-related protein associated protein 1
Chromosome 5
Gene Common Name(s) A2MRAP; A2RAP; AA617339; AI790446; AU042172; C77774; HBP44; MRAP; MYP23; RAP; alpha-2-MRAP; expressed sequence AA617339; expressed sequence AI790446; expressed sequence AU042172; expressed sequence C77774;
General Note ES cell line = JH1 or AB1 (129S7/SvEvBrd-Hprt+).
Molecular Note A neomycin resistance cassette was inserted into sequences that encode the leader peptide. Immunoblot analysis of liver and brain did not detect the encoded protein in homozygous animals. [MGI Ref ID J:43736]

Genotyping

Genotyping Information

Genotyping Protocols

Lrpaptm1Her, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Willnow TE; Armstrong SA; Hammer RE; Herz J. 1995. Functional expression of low density lipoprotein receptor-related protein is controlled by receptor-associated protein in vivo. Proc Natl Acad Sci U S A 92(10):4537-41. [PubMed: 7538675]  [MGI Ref ID J:43736]

Additional References

Willnow TE; Rohlmann A; Horton J; Otani H; Braun JR; Hammer RE; Herz J. 1996. RAP, a specialized chaperone, prevents ligand-induced ER retention and degradation of LDL receptor-related endocytic receptors. EMBO J 15(11):2632-9. [PubMed: 8654360]  [MGI Ref ID J:33584]

Lrpap1tm1Her related

Bacic D; Capuano P; Gisler SM; Pribanic S; Christensen EI; Biber J; Loffing J; Kaissling B; Wagner CA; Murer H. 2003. Impaired PTH-induced endocytotic down-regulation of the renal type IIa Na+/Pi-cotransporter in RAP-deficient mice with reduced megalin expression. Pflugers Arch 446(4):475-84. [PubMed: 12748857]  [MGI Ref ID J:106203]

Deane R; Sagare A; Hamm K; Parisi M; LaRue B; Guo H; Wu Z; Holtzman DM; Zlokovic BV. 2005. IgG-assisted age-dependent clearance of Alzheimer's amyloid beta peptide by the blood-brain barrier neonatal Fc receptor. J Neurosci 25(50):11495-503. [PubMed: 16354907]  [MGI Ref ID J:103998]

Licastro F; Campbell IL; Kincaid C; Veinbergs I; Van Uden E; Rockenstein E; Mallory M; Gilbert JR; Masliah E. 1999. A role for apoE in regulating the levels of alpha-1-antichymotrypsin in the aging mouse brain and in Alzheimer's disease. Am J Pathol 155(3):869-75. [PubMed: 10487844]  [MGI Ref ID J:57495]

Lisi S; Botta R; Pinchera A; Collins AB; Refetoff S; Arvan P; Bu G; Grasso L; Marshansky V; Bechoua S; Hurtado-Lorenzo A; Marcocci C; Brown D; McCluskey RT; Marino M. 2006. Defective thyroglobulin storage in LDL receptor-associated protein-deficient mice. Am J Physiol Cell Physiol 290(4):C1160-7. [PubMed: 16306127]  [MGI Ref ID J:107358]

Marschang P; Brich J; Weeber EJ; Sweatt JD; Shelton JM; Richardson JA; Hammer RE; Herz J. 2004. Normal development and fertility of knockout mice lacking the tumor suppressor gene LRP1b suggest functional compensation by LRP1. Mol Cell Biol 24(9):3782-93. [PubMed: 15082773]  [MGI Ref ID J:90197]

Martins IJ; Hone E; Chi C; Seydel U; Martins RN; Redgrave TG. 2000. Relative roles of LDLr and LRP in the metabolism of chylomicron remnants in genetically manipulated mice. J Lipid Res 41(2):205-13. [PubMed: 10681404]  [MGI Ref ID J:120545]

Singh I; Sagare AP; Coma M; Perlmutter D; Gelein R; Bell RD; Deane RJ; Zhong E; Parisi M; Ciszewski J; Kasper RT; Deane R. 2013. Low levels of copper disrupt brain amyloid-beta homeostasis by altering its production and clearance. Proc Natl Acad Sci U S A 110(36):14771-6. [PubMed: 23959870]  [MGI Ref ID J:200976]

Umans L; Serneels L; Lorent K; Dewachter I; Tesseur I; Moechars D; Van Leuven F. 1999. Lipoprotein receptor-related protein in brain and in cultured neurons of mice deficient in receptor-associated protein and transgenic for apolipoprotein E4 or amyloid precursor protein. Neuroscience 94(1):315-21. [PubMed: 10613521]  [MGI Ref ID J:59778]

Van Uden E; Mallory M; Veinbergs I; Alford M; Rockenstein E; Masliah E. 2002. Increased extracellular amyloid deposition and neurodegeneration in human amyloid precursor protein transgenic mice deficient in receptor-associated protein. J Neurosci 22(21):9298-304. [PubMed: 12417655]  [MGI Ref ID J:107567]

Van Uden E; Veinbergs I; Mallory M; Orlando R; Masliah E. 1999. A novel role for receptor-associated protein in somatostatin modulation: implications for Alzheimer's disease. Neuroscience 88(3):687-700. [PubMed: 10363810]  [MGI Ref ID J:107552]

Veinbergs I; Van Uden E; Mallory M; Alford M; McGiffert C; DeTeresa R; Orlando R; Masliah E. 2001. Role of apolipoprotein E receptors in regulating the differential in vivo neurotrophic effects of apolipoprotein E. Exp Neurol 170(1):15-26. [PubMed: 11421580]  [MGI Ref ID J:70482]

Willnow TE; Rohlmann A; Horton J; Otani H; Braun JR; Hammer RE; Herz J. 1996. RAP, a specialized chaperone, prevents ligand-induced ER retention and degradation of LDL receptor-related endocytic receptors. EMBO J 15(11):2632-9. [PubMed: 8654360]  [MGI Ref ID J:33584]

Xu G; Karch C; Li N; Lin N; Fromholt D; Gonzales V; Borchelt DR. 2008. Receptor-associated protein (RAP) plays a central role in modulating Abeta deposition in APP/PS1 transgenic mice. PLoS ONE 3(9):e3159. [PubMed: 18776935]  [MGI Ref ID J:143942]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen held in a live colony, this strain may be maintained by homozygous siblings matings. Expected coat color from breeding:Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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