Strain Name:

B6;129S7-Inhbatm1Zuk/J

Stock Number:

002990

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Martin Matzuk,   Baylor College of Medicine

Description
Homozygous mice die within 24 hours of birth. They show craniofacial defects including a cleft palate.

Development
Exon 2 of the Inhba gene was replaced with a vector containing Hprt. The 129-derived AB2.1 ES cell line was used.

Control Information

  Control
   See control note: Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls The B6129SF2 mice only provide an approximate match to this mixed B6,129 background.
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
007664   129S-Efnb1tm1Sor/J
000646   A/J
000647   A/WySnJ
005709   B6.129-Skitm1Cco/J
002619   B6.129-Tgfb3tm1Doe/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010546   B6.129S1-Jag2tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
002711   B6;129-Gabrb3tm1Geh/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010618   B6;129S-Jag1tm2Grid/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
022358   B6;129S6-Rr23tm1Axvi/Mmjax
022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCeb/OveJ
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
003318   STOCK Shhtm1Amc/J
003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
008469   STOCK Wnt9btm1.2Amc/J
View Facebase: models     (61 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Inhbatm1Zuk/Inhbatm1Zuk

        either: (129S/SvEv-Inhbatm1Zuk) or (involves: 129S7/SvEvBrd * C57BL/6)
  • mortality/aging
  • complete neonatal lethality
    • mice die within 24 hours of birth   (MGI Ref ID J:23923)
  • craniofacial phenotype
  • abnormal alveolar process morphology
    • mice display a secondary defect in the alveolar ridge of the mandible   (MGI Ref ID J:23923)
  • absent hard palate
    • 7 of 22 mice (32%) on a mixed background that do not display a cleft palate lack a hard palate   (MGI Ref ID J:23923)
  • absent incisors   (MGI Ref ID J:23923)
    • absent lower incisors
      • lower incisors are completely absent from 22 of 25 mice   (MGI Ref ID J:23923)
  • cleft secondary palate
    • 12 of 42 (29%) of mice on a mixed background while 3 of 9 (33%) of mice on an inbred background have a cleft in the secondary palate   (MGI Ref ID J:23923)
  • behavior/neurological phenotype
  • no suckling reflex   (MGI Ref ID J:23923)
  • skeleton phenotype
  • abnormal alveolar process morphology
    • mice display a secondary defect in the alveolar ridge of the mandible   (MGI Ref ID J:23923)
  • digestive/alimentary phenotype
  • absent hard palate
    • 7 of 22 mice (32%) on a mixed background that do not display a cleft palate lack a hard palate   (MGI Ref ID J:23923)
  • cleft secondary palate
    • 12 of 42 (29%) of mice on a mixed background while 3 of 9 (33%) of mice on an inbred background have a cleft in the secondary palate   (MGI Ref ID J:23923)
  • integument phenotype
  • abnormal vibrissa follicle morphology
    • growth and/or differentiation of whisker follicles is delayed   (MGI Ref ID J:23923)
    • if present, the root sheath is immature   (MGI Ref ID J:23923)
    • cells at the base of the hair bulb are irregular in pattern   (MGI Ref ID J:23923)
    • papilla lies closer to the surface   (MGI Ref ID J:23923)
  • absent vibrissae
    • mice are born without vibrissae   (MGI Ref ID J:23923)
  • growth/size/body phenotype
  • absent hard palate
    • 7 of 22 mice (32%) on a mixed background that do not display a cleft palate lack a hard palate   (MGI Ref ID J:23923)
  • absent incisors   (MGI Ref ID J:23923)
    • absent lower incisors
      • lower incisors are completely absent from 22 of 25 mice   (MGI Ref ID J:23923)
  • cleft secondary palate
    • 12 of 42 (29%) of mice on a mixed background while 3 of 9 (33%) of mice on an inbred background have a cleft in the secondary palate   (MGI Ref ID J:23923)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Inhbatm1Zuk/Inhba+

        B6.129S7-Inhbatm1Zuk
  • muscle phenotype
  • abnormal pectoral muscle morphology
    • increased weight in males and females   (MGI Ref ID J:182827)
  • increased gastrocnemius weight
    • in males and females   (MGI Ref ID J:182827)
  • increased quadriceps weight
    • in males and females   (MGI Ref ID J:182827)
  • increased skeletal muscle weight
    • increased triceps weight in males and females   (MGI Ref ID J:182827)
  • growth/size/body phenotype
  • increased body weight

Inhbatm1Zuk/Inhbatm1Zuk

        involves: 129S7/SvEvBrd
  • reproductive system phenotype
  • abnormal epididymis morphology
    • epididymis fails to develop the characteristic coiling in the epithelium   (MGI Ref ID J:122819)
    • however, testosterone production and action is normal   (MGI Ref ID J:122819)
    • abnormal epididymis epithelium morphology
      • the typical increase in proliferation of cells in the Wolffian duct epithelium as it transforms into the epididymis from E15.5 to E19.5 is absent in mutants   (MGI Ref ID J:122819)
  • embryogenesis phenotype
  • abnormal Wolffian duct morphology
    • Wolffian duct fails to elongate and coil, leaving a straight duct in the mesonephros   (MGI Ref ID J:122819)
    • the epithelial layer of the Wolffian duct becomes high columnar in shape compared to high cuboidal to low columnar in wild-type, and cell width decreases as the epithelial layer becomes crowded   (MGI Ref ID J:122819)
    • mesenchymal cells surrounding the duct are condensed and disorganized   (MGI Ref ID J:122819)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Inhbatm1Zuk related

Developmental Biology Research
Craniofacial and Palate Defects
Postnatal Lethality
      Homozygous

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Inhbatm1Zuk
Allele Name targeted mutation 1, Martin M Matzuk
Allele Type Targeted (Null/Knockout)
Common Name(s) Inhba-; actbetaAml;
Mutation Made ByDr. Martin Matzuk,   Baylor College of Medicine
Strain of Origin129S7/SvEvBrd-Hprt
ES Cell Line NameAB2.1
ES Cell Line Strain129S7/SvEvBrd-Hprt
Gene Symbol and Name Inhba, inhibin beta-A
Chromosome 13
Gene Common Name(s) EDF; FRP; activin;
Molecular Note A PGK hprt cassette replaced exon 2 of the gene. [MGI Ref ID J:23923]

Genotyping

Genotyping Information

Genotyping Protocols

Inhbatm1Zuk-Alternate 1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Matzuk MM; Kumar TR; Vassalli A; Bickenbach JR; Roop DR; Jaenisch R; Bradley A. 1995. Functional analysis of activins during mammalian development [see comments] Nature 374(6520):354-6. [PubMed: 7885473]  [MGI Ref ID J:23923]

Additional References

Inhbatm1Zuk related

Archambeault DR; Tomaszewski J; Childs AJ; Anderson RA; Yao HH. 2011. Testicular somatic cells, not gonocytes, are the major source of functional activin A during testis morphogenesis. Endocrinology 152(11):4358-67. [PubMed: 21952240]  [MGI Ref ID J:178873]

Archambeault DR; Yao HH. 2010. Activin A, a product of fetal Leydig cells, is a unique paracrine regulator of Sertoli cell proliferation and fetal testis cord expansion. Proc Natl Acad Sci U S A 107(23):10526-31. [PubMed: 20498064]  [MGI Ref ID J:161291]

Brown CW; Houston-Hawkins DE; Woodruff TK; Matzuk MM. 2000. Insertion of Inhbb into the Inhba locus rescues the Inhba-null phenotype and reveals new activin functions. Nat Genet 25(4):453-7. [PubMed: 10932194]  [MGI Ref ID J:63882]

Brown CW; Li L; Houston-Hawkins DE; Matzuk MM. 2003. Activins are critical modulators of growth and survival. Mol Endocrinol 17(12):2404-17. [PubMed: 14551263]  [MGI Ref ID J:86858]

Davis AA; Matzuk MM; Reh TA. 2000. Activin A promotes progenitor differentiation into photoreceptors in rodent retina. Mol Cell Neurosci 15(1):11-21. [PubMed: 10662502]  [MGI Ref ID J:124431]

Ferguson CA; Tucker AS; Christensen L; Lau AL; Matzuk MM; Sharpe PT. 1998. Activin is an essential early mesenchymal signal in tooth development that is required for patterning of the murine dentition. Genes Dev 12(16):2636-49. [PubMed: 9716414]  [MGI Ref ID J:49552]

Jhaveri S; Erzurumlu RS; Chiaia N; Kumar TR; Matzuk MM. 1998. Defective whisker follicles and altered brainstem patterns in activin and follistatin knockout mice. Mol Cell Neurosci 12(4-5):206-19. [PubMed: 9828086]  [MGI Ref ID J:50975]

Lee SJ; Lee YS; Zimmers TA; Soleimani A; Matzuk MM; Tsuchida K; Cohn RD; Barton ER. 2010. Regulation of muscle mass by follistatin and activins. Mol Endocrinol 24(10):1998-2008. [PubMed: 20810712]  [MGI Ref ID J:182827]

Li L; Shen JJ; Bournat JC; Huang L; Chattopadhyay A; Li Z; Shaw C; Graham BH; Brown CW. 2009. Activin signaling: effects on body composition and mitochondrial energy metabolism. Endocrinology 150(8):3521-9. [PubMed: 19389832]  [MGI Ref ID J:158155]

Li Q; Karam SM; Coerver KA; Matzuk MM; Gordon JI. 1998. Stimulation of activin receptor II signaling pathways inhibits differentiation of multiple gastric epithelial lineages. Mol Endocrinol 12(2):181-92. [PubMed: 9482661]  [MGI Ref ID J:110642]

Pangas SA. 2012. Regulation of the ovarian reserve by members of the transforming growth factor beta family. Mol Reprod Dev 79(10):666-79. [PubMed: 22847922]  [MGI Ref ID J:190579]

Pangas SA; Jorgez CJ; Tran M; Agno J; Li X; Brown CW; Kumar TR; Matzuk MM. 2007. Intraovarian activins are required for female fertility. Mol Endocrinol 21(10):2458-71. [PubMed: 17609433]  [MGI Ref ID J:125354]

Simmons DG; Cross JC. 2005. Determinants of trophoblast lineage and cell subtype specification in the mouse placenta. Dev Biol 284(1):12-24. [PubMed: 15963972]  [MGI Ref ID J:100602]

Tomaszewski J; Joseph A; Archambeault D; Yao HH. 2007. Essential roles of inhibin beta A in mouse epididymal coiling. Proc Natl Acad Sci U S A 104(27):11322-7. [PubMed: 17592132]  [MGI Ref ID J:122819]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   See control note: Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls The B6129SF2 mice only provide an approximate match to this mixed B6,129 background.
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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