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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+3
Generation DefinitionsDonating Investigator Dr. Martin Matzuk, Baylor College of Medicine Description
Homozygous mice die within 24 hours of birth. They show craniofacial defects including a cleft palate.Development
Exon 2 of the Inhba gene was replaced with a vector containing Hprt. The 129-derived AB2.1 ES cell line was used.
Control Information
| Control | ||
|---|---|---|
| See control note: | Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls The B6129SF2 mice only provide an approximate match to this mixed B6,129 background. | |
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: models
View Facebase: models (58 strains)
Phenotype
Phenotype Information
assigned by genotype
Inhbatm1Zuk/Inhbatm1Zuk
either: (129S/SvEv-Inhbatm1Zuk) or (involves: 129S7/SvEvBrd * C57BL/6)
- mortality/aging
- complete neonatal lethality
- mice die within 24 hours of birth (MGI Ref ID J:23923)
- craniofacial phenotype
- abnormal alveolar process morphology
- mice display a secondary defect in the alveolar ridge of the mandible (MGI Ref ID J:23923)
- absent hard palate
- 7 of 22 mice (32%) on a mixed background that do not display a cleft palate lack a hard palate (MGI Ref ID J:23923)
- absent incisors (MGI Ref ID J:23923)
- absent lower incisors
- lower incisors are completely absent from 22 of 25 mice (MGI Ref ID J:23923)
- cleft secondary palate
- 12 of 42 (29%) of mice on a mixed background while 3 of 9 (33%) of mice on an inbred background have a cleft in the secondary palate (MGI Ref ID J:23923)
- behavior/neurological phenotype
- no suckling reflex (MGI Ref ID J:23923)
- skeleton phenotype
- abnormal alveolar process morphology
- mice display a secondary defect in the alveolar ridge of the mandible (MGI Ref ID J:23923)
- digestive/alimentary phenotype
- absent hard palate
- 7 of 22 mice (32%) on a mixed background that do not display a cleft palate lack a hard palate (MGI Ref ID J:23923)
- cleft secondary palate
- 12 of 42 (29%) of mice on a mixed background while 3 of 9 (33%) of mice on an inbred background have a cleft in the secondary palate (MGI Ref ID J:23923)
- integument phenotype
- abnormal vibrissa follicle morphology
- absent vibrissae
- mice are born without vibrissae (MGI Ref ID J:23923)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Inhbatm1Zuk/Inhba+
B6.129S7-Inhbatm1Zuk
- muscle phenotype
- abnormal pectoral muscle morphology
- increased weight in males and females (MGI Ref ID J:182827)
- increased gastrocnemius weight
- in males and females (MGI Ref ID J:182827)
- increased quadriceps weight
- in males and females (MGI Ref ID J:182827)
- increased skeletal muscle weight
- increased triceps weight in males and females (MGI Ref ID J:182827)
- growth/size phenotype
- increased body weight
- in males (MGI Ref ID J:182827)
Inhbatm1Zuk/Inhbatm1Zuk
involves: 129S7/SvEvBrd
- reproductive system phenotype
- abnormal epididymis morphology
- abnormal epididymis epithelium morphology
- the typical increase in proliferation of cells in the Wolffian duct epithelium as it transforms into the epididymis from E15.5 to E19.5 is absent in mutants (MGI Ref ID J:122819)
- embryogenesis phenotype
- abnormal Wolffian duct morphology
- Wolffian duct fails to elongate and coil, leaving a straight duct in the mesonephros (MGI Ref ID J:122819)
- the epithelial layer of the Wolffian duct becomes high columnar in shape compared to high cuboidal to low columnar in wild-type, and cell width decreases as the epithelial layer becomes crowded (MGI Ref ID J:122819)
- mesenchymal cells surrounding the duct are condensed and disorganized (MGI Ref ID J:122819)
This mouse can be used to support research in many areas including:Inhbatm1Zuk related
Developmental Biology Research
Craniofacial and Palate Defects
Postnatal Lethality
Homozygous
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Inhbatm1Zuk | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Martin M Matzuk | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Inhba-; actbetaAml; | ||
| Mutation Made By | Dr. Martin Matzuk, Baylor College of Medicine | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt | ||
| ES Cell Line Name | AB2.1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt | ||
| Gene Symbol and Name | Inhba, inhibin beta-A | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | EDF; FRP; activin; | ||
| Molecular Note | A PGK hprt cassette replaced exon 2 of the gene. [MGI Ref ID J:23923] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic HPRT_Alternative,Separated MCA
Generic HPRT_Alternative, Separated PCR
Human HPRT/KO2, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Matzuk MM; Kumar TR; Vassalli A; Bickenbach JR; Roop DR; Jaenisch R; Bradley A. 1995. Functional analysis of activins during mammalian development [see comments] Nature 374(6520):354-6. [PubMed: 7885473] [MGI Ref ID J:23923]
Additional References
Inhbatm1Zuk relatedArchambeault DR; Tomaszewski J; Childs AJ; Anderson RA; Yao HH. 2011. Testicular somatic cells, not gonocytes, are the major source of functional activin A during testis morphogenesis. Endocrinology 152(11):4358-67. [PubMed: 21952240] [MGI Ref ID J:178873]
Archambeault DR; Yao HH. 2010. Activin A, a product of fetal Leydig cells, is a unique paracrine regulator of Sertoli cell proliferation and fetal testis cord expansion. Proc Natl Acad Sci U S A 107(23):10526-31. [PubMed: 20498064] [MGI Ref ID J:161291]
Brown CW; Houston-Hawkins DE; Woodruff TK; Matzuk MM. 2000. Insertion of Inhbb into the Inhba locus rescues the Inhba-null phenotype and reveals new activin functions. Nat Genet 25(4):453-7. [PubMed: 10932194] [MGI Ref ID J:63882]
Brown CW; Li L; Houston-Hawkins DE; Matzuk MM. 2003. Activins are critical modulators of growth and survival. Mol Endocrinol 17(12):2404-17. [PubMed: 14551263] [MGI Ref ID J:86858]
Davis AA; Matzuk MM; Reh TA. 2000. Activin A promotes progenitor differentiation into photoreceptors in rodent retina. Mol Cell Neurosci 15(1):11-21. [PubMed: 10662502] [MGI Ref ID J:124431]
Ferguson CA; Tucker AS; Christensen L; Lau AL; Matzuk MM; Sharpe PT. 1998. Activin is an essential early mesenchymal signal in tooth development that is required for patterning of the murine dentition. Genes Dev 12(16):2636-49. [PubMed: 9716414] [MGI Ref ID J:49552]
Jhaveri S; Erzurumlu RS; Chiaia N; Kumar TR; Matzuk MM. 1998. Defective whisker follicles and altered brainstem patterns in activin and follistatin knockout mice. Mol Cell Neurosci 12(4-5):206-19. [PubMed: 9828086] [MGI Ref ID J:50975]
Lee SJ; Lee YS; Zimmers TA; Soleimani A; Matzuk MM; Tsuchida K; Cohn RD; Barton ER. 2010. Regulation of muscle mass by follistatin and activins. Mol Endocrinol 24(10):1998-2008. [PubMed: 20810712] [MGI Ref ID J:182827]
Li L; Shen JJ; Bournat JC; Huang L; Chattopadhyay A; Li Z; Shaw C; Graham BH; Brown CW. 2009. Activin signaling: effects on body composition and mitochondrial energy metabolism. Endocrinology 150(8):3521-9. [PubMed: 19389832] [MGI Ref ID J:158155]
Li Q; Karam SM; Coerver KA; Matzuk MM; Gordon JI. 1998. Stimulation of activin receptor II signaling pathways inhibits differentiation of multiple gastric epithelial lineages. Mol Endocrinol 12(2):181-92. [PubMed: 9482661] [MGI Ref ID J:110642]
Pangas SA. 2012. Regulation of the ovarian reserve by members of the transforming growth factor beta family. Mol Reprod Dev 79(10):666-79. [PubMed: 22847922] [MGI Ref ID J:190579]
Pangas SA; Jorgez CJ; Tran M; Agno J; Li X; Brown CW; Kumar TR; Matzuk MM. 2007. Intraovarian activins are required for female fertility. Mol Endocrinol 21(10):2458-71. [PubMed: 17609433] [MGI Ref ID J:125354]
Simmons DG; Cross JC. 2005. Determinants of trophoblast lineage and cell subtype specification in the mouse placenta. Dev Biol 284(1):12-24. [PubMed: 15963972] [MGI Ref ID J:100602]
Tomaszewski J; Joseph A; Archambeault D; Yao HH. 2007. Essential roles of inhibin beta A in mouse epididymal coiling. Proc Natl Acad Sci U S A 104(27):11322-7. [PubMed: 17592132] [MGI Ref ID J:122819]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3000.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| See control note: | Wildtype mice from the colony or B6129SF2 mice (Stock No. 101045) may be used as controls The B6129SF2 mice only provide an approximate match to this mixed B6,129 background. | |
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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