Strain Name:

B6.129-Ascl1tm1And/J

Stock Number:

002991

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
 
Donating Investigator David J Anderson,   California Institute of Technology, HHMI

Description
Mice homozygous for the Ascl1tm1And targeted mutation die within 24 hours after birth. There are no gross external defects; However, mutant mice do have extensive loss of sympathetic ganglia and olefactory epithelium.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ascl1
012882   STOCK Ascl1tm1.1(Cre/ERT2)Jejo/J
012881   STOCK Ascl1tm1Reed/J
View Strains carrying other alleles of Ascl1     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Central Hypoventilation Syndrome, Congenital; CCHS   (ASCL1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ascl1tm1And/Ascl1+

        involves: 129/Sv
  • nervous system phenotype
  • decreased solitary pulmonary neuroendocrine cell number
    • the number of CGRP-positive pulmonary neuroendocrine cells (including single and clustered PNECs) is significantly reduced in E13-E17 and neonatal heterozygous mutant lungs relative to that in wild-type lungs   (MGI Ref ID J:64088)
  • respiratory system phenotype
  • decreased number of pulmonary neuroendocrine bodies
    • the number of clustered PNECs is significantly reduced in E13-E17 and neonatal heterozygous mutant lungs relative to that in wild-type lungs   (MGI Ref ID J:64088)
  • endocrine/exocrine gland phenotype
  • decreased solitary pulmonary neuroendocrine cell number
    • the number of CGRP-positive pulmonary neuroendocrine cells (including single and clustered PNECs) is significantly reduced in E13-E17 and neonatal heterozygous mutant lungs relative to that in wild-type lungs   (MGI Ref ID J:64088)

Ascl1tm1And/Ascl1tm1And

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • mortality/aging
  • complete neonatal lethality
    • animals die within 24 hours of birth   (MGI Ref ID J:15850)
  • pigmentation phenotype
  • abnormal skin pigmentation
    • gray appearance in 25% of animals   (MGI Ref ID J:15850)
  • behavior/neurological phenotype
  • absent gastric milk in neonates
    • neonates did not feed; no milk in stomach after 6-12 hours   (MGI Ref ID J:15850)
  • respiratory system phenotype
  • abnormal olfactory epithelium morphology
    • thinner than normal   (MGI Ref ID J:15850)
    • abnormal olfactory sensory neuron morphology
      • absent olfactory neurons; very few remaining cells in epithelium; precursors do not form   (MGI Ref ID J:15850)
      • normal sustentacular cells   (MGI Ref ID J:15850)
  • respiratory distress
    • difficulty in breathing, deep gasping movements apparent   (MGI Ref ID J:15850)
  • taste/olfaction phenotype
  • abnormal olfactory epithelium morphology
    • thinner than normal   (MGI Ref ID J:15850)
    • abnormal olfactory sensory neuron morphology
      • absent olfactory neurons; very few remaining cells in epithelium; precursors do not form   (MGI Ref ID J:15850)
      • normal sustentacular cells   (MGI Ref ID J:15850)
  • nervous system phenotype
  • abnormal enteric neuron morphology
    • delayed myenteric neuron development   (MGI Ref ID J:15850)
    • absent enteric neurons   (MGI Ref ID J:15850)
  • abnormal olfactory sensory neuron morphology
    • absent olfactory neurons; very few remaining cells in epithelium; precursors do not form   (MGI Ref ID J:15850)
    • normal sustentacular cells   (MGI Ref ID J:15850)
  • abnormal parasympathetic ganglion morphology   (MGI Ref ID J:15850)
  • abnormal sympathetic ganglion morphology   (MGI Ref ID J:15850)
  • craniofacial phenotype
  • abnormal olfactory epithelium morphology
    • thinner than normal   (MGI Ref ID J:15850)
    • abnormal olfactory sensory neuron morphology
      • absent olfactory neurons; very few remaining cells in epithelium; precursors do not form   (MGI Ref ID J:15850)
      • normal sustentacular cells   (MGI Ref ID J:15850)
  • integument phenotype
  • abnormal skin pigmentation
    • gray appearance in 25% of animals   (MGI Ref ID J:15850)
  • growth/size/body phenotype
  • abnormal olfactory epithelium morphology
    • thinner than normal   (MGI Ref ID J:15850)
    • abnormal olfactory sensory neuron morphology
      • absent olfactory neurons; very few remaining cells in epithelium; precursors do not form   (MGI Ref ID J:15850)
      • normal sustentacular cells   (MGI Ref ID J:15850)

Ascl1tm1And/Ascl1tm1And

        involves: 129S1/Sv * 129X1/SvJ
  • mortality/aging
  • complete neonatal lethality
    • animals die within 24 hours of birth   (MGI Ref ID J:15850)

Ascl1tm1And/Ascl1tm1And

        involves: 129/Sv
  • nervous system phenotype
  • abnormal neuron apoptosis
    • at E11.5 and E12.5, numbers of TUNEL positive cells in the spinal cords of mutants are increased by factors of 3.2 and 9.7 respectively compared to controls, and apoptotic cells are seen primarily in the ventricular zone   (MGI Ref ID J:109482)
  • abnormal neuronal precursor proliferation
    • decreased proliferation in culture   (MGI Ref ID J:203419)
  • abnormal spinal cord interneuron morphology
    • in the dorsal spinal cord at E12.5,ratio of dILA to dILB interneurons in the dorsal spinal cord is severely reduced   (MGI Ref ID J:109482)
    • at E11.5 and subsequent stages, the numbers on newly generated dILA interneurons seen in the dorsal spinal cord are significantly reduced   (MGI Ref ID J:109482)
    • V2 interneuron numbers are reduced to a similar extent as Ascl1tm1And   (MGI Ref ID J:74525)
    • index of differentiation is reduced in the spinal cord at E11.5 compared to wild-type   (MGI Ref ID J:109482)
    • at E10.5 embryos show a 70% loss of dI3 and a complete loss of dI5 neurons   (MGI Ref ID J:98830)
    • in E10.5 embryos a dramatic increase in dI2 and dI4/6 interneuron number is seen   (MGI Ref ID J:98830)
  • abnormal telencephalon development
    • mutants show a defect in ventral telencephalic progenitors; progenitors are missing at E12.5   (MGI Ref ID J:74525)
  • absent solitary pulmonary neuroendocrine cells
    • newborn mutants show complete lack of solitary airway neuroendocrine (NE) cells, as characterized by a panel of NE markers   (MGI Ref ID J:113000)
    • in contrast, enteric neurons and NE cells in the gut and pancreatic islets develop normally   (MGI Ref ID J:113000)
    • no CGRP-positive pulmonary neuroendocrine cells (including single and clustered PNECs) are detected in E13-E17 or neonatal lungs   (MGI Ref ID J:64088)
  • impaired neuron differentiation
    • cultured precursor cells fail to produce neurons   (MGI Ref ID J:203419)
  • respiratory system phenotype
  • abnormal lung development
    • newborn mutants show disruption of pulmonary neuroendocrine cell differentiation   (MGI Ref ID J:113000)
    • however, other bronchopulmonary cell types (e.g. Clara cells and type II alveolar cells) appear normal   (MGI Ref ID J:113000)
  • absent pulmonary neuroendocrine bodies
    • newborn mutants show complete lack of airway neuroepithelial bodies (normally clustering at airway branch points), as characterized by a panel of neuroendocrine markers   (MGI Ref ID J:113000)
    • no clustered PNECs are detected in E13-E17 or neonatal lungs   (MGI Ref ID J:64088)
  • endocrine/exocrine gland phenotype
  • absent solitary pulmonary neuroendocrine cells
    • newborn mutants show complete lack of solitary airway neuroendocrine (NE) cells, as characterized by a panel of NE markers   (MGI Ref ID J:113000)
    • in contrast, enteric neurons and NE cells in the gut and pancreatic islets develop normally   (MGI Ref ID J:113000)
    • no CGRP-positive pulmonary neuroendocrine cells (including single and clustered PNECs) are detected in E13-E17 or neonatal lungs   (MGI Ref ID J:64088)
  • cellular phenotype
  • abnormal neuron apoptosis
    • at E11.5 and E12.5, numbers of TUNEL positive cells in the spinal cords of mutants are increased by factors of 3.2 and 9.7 respectively compared to controls, and apoptotic cells are seen primarily in the ventricular zone   (MGI Ref ID J:109482)
  • abnormal neuronal precursor proliferation
    • decreased proliferation in culture   (MGI Ref ID J:203419)
  • impaired neuron differentiation
    • cultured precursor cells fail to produce neurons   (MGI Ref ID J:203419)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Ascl1tm1And related

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Neural Tube Defects
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ascl1tm1And
Allele Name targeted mutation 1, David J Anderson
Allele Type Targeted (Null/Knockout)
Common Name(s) Ascl1delta; Mash-1-; Mash-1delta; Mash1-;
Mutation Made By David Anderson,   California Institute of Technology, HHMI
Strain of Origin129/Sv
ES Cell Line NameOther (see notes)
ES Cell Line Strain129
Gene Symbol and Name Ascl1, achaete-scute complex homolog 1 (Drosophila)
Chromosome 10
Gene Common Name(s) AI225900; ASH1; HASH1; MASH1; Mash1; bHLHa46; expressed sequence AI225900; mammalian achaete scute homolog 1;
General Note ES cell line = D3 (129S2/SvPas) or R1 (129S1/Sv x 129X1/SvJ)F1. The authors state that most phenotypic analysis was done on R1-derived mice.
Molecular Note A PGK-neomycin resistance cassette replaced the entire coding region, 0.6 kb of sequence 5' of the translation initiation codon, and 0.2 kb of sequence 3' of the translation termination codon. [MGI Ref ID J:15850]

Genotyping

Genotyping Information

Genotyping Protocols

NEOTD (Generic Neo), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Guillemot F; Caspary T; Tilghman SM; Copeland NG; Gilbert DJ; Jenkins NA; Anderson DJ; Joyner AL; Rossant J; Nagy A. 1995. Genomic imprinting of Mash2, a mouse gene required for trophoblast development. Nat Genet 9(3):235-42. [PubMed: 7773285]  [MGI Ref ID J:23260]

Additional References

Lo L; Tiveron MC; Anderson DJ. 1998. MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity. Development 125(4):609-20. [PubMed: 9435282]  [MGI Ref ID J:46371]

Ascl1tm1And related

Akagi T; Inoue T; Miyoshi G; Bessho Y; Takahashi M; Lee JE; Guillemot F; Kageyama R. 2004. Requirement of multiple basic helix-loop-helix genes for retinal neuronal subtype specification. J Biol Chem 279(27):28492-8. [PubMed: 15105417]  [MGI Ref ID J:121047]

Battiste J; Helms AW; Kim EJ; Savage TK; Lagace DC; Mandyam CD; Eisch AJ; Miyoshi G; Johnson JE. 2007. Ascl1 defines sequentially generated lineage-restricted neuronal and oligodendrocyte precursor cells in the spinal cord. Development 134(2):285-93. [PubMed: 17166924]  [MGI Ref ID J:117032]

Blaugrund E; Pham TD; Tennyson VM; Lo L; Sommer L; Anderson DJ; Gershon MD. 1996. Distinct subpopulations of enteric neuronal progenitors defined by time of development, sympathoadrenal lineage markers and Mash-1-dependence. Development 122(1):309-20. [PubMed: 8565843]  [MGI Ref ID J:81634]

Borges M; Linnoila RI; van de Velde HJ; Chen H; Nelkin BD; Mabry M; Baylin SB; Ball DW. 1997. An achaete-scute homologue essential for neuroendocrine differentiation in the lung. Nature 386(6627):852-5. [PubMed: 9126746]  [MGI Ref ID J:113000]

Britz O; Mattar P; Nguyen L; Langevin LM; Zimmer C; Alam S; Guillemot F; Schuurmans C. 2006. A role for proneural genes in the maturation of cortical progenitor cells. Cereb Cortex 16 Suppl 1:i138-51. [PubMed: 16766700]  [MGI Ref ID J:174488]

Casarosa S; Fode C; Guillemot F. 1999. Mash1 regulates neurogenesis in the ventral telencephalon. Development 126(3):525-34. [PubMed: 9876181]  [MGI Ref ID J:51598]

Castro DS; Martynoga B; Parras C; Ramesh V; Pacary E; Johnston C; Drechsel D; Lebel-Potter M; Garcia LG; Hunt C; Dolle D; Bithell A; Ettwiller L; Buckley N; Guillemot F. 2011. A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets. Genes Dev 25(9):930-45. [PubMed: 21536733]  [MGI Ref ID J:171415]

Cau E; Casarosa S; Guillemot F. 2002. Mash1 and Ngn1 control distinct steps of determination and differentiation in the olfactory sensory neuron lineage. Development 129(8):1871-80. [PubMed: 11934853]  [MGI Ref ID J:75936]

Cau E; Gradwohl G; Fode C; Guillemot F. 1997. Mash1 activates a cascade of bHLH regulators in olfactory neuron progenitors. Development 124(8):1611-1621. [PubMed: 9108377]  [MGI Ref ID J:40190]

Chapman H; Waclaw RR; Pei Z; Nakafuku M; Campbell K. 2013. The homeobox gene Gsx2 controls the timing of oligodendroglial fate specification in mouse lateral ganglionic eminence progenitors. Development 140(11):2289-98. [PubMed: 23637331]  [MGI Ref ID J:198563]

Dauger S; Guimiot F; Renolleau S; Levacher B; Boda B; Mas C; Nepote V; Simonneau M; Gaultier C; Gallego J. 2001. MASH-1/RET pathway involvement in development of brain stem control of respiratory frequency in newborn mice. Physiol Genomics 7(2):149-57. [PubMed: 11773601]  [MGI Ref ID J:124459]

Dauger S; Renolleau S; Vardon G; Nepote V; Mas C; Simonneau M; Gaultier C; Gallego J. 1999. Ventilatory responses to hypercapnia and hypoxia in Mash-1 heterozygous newborn and adult mice. Pediatr Res 46(5):535-42. [PubMed: 10541315]  [MGI Ref ID J:59820]

Del Barrio MG; Taveira-Marques R; Muroyama Y; Yuk DI; Li S; Wines-Samuelson M; Shen J; Smith HK; Xiang M; Rowitch D; Richardson WD. 2007. A regulatory network involving Foxn4, Mash1 and delta-like 4/Notch1 generates V2a and V2b spinal interneurons from a common progenitor pool. Development 134(19):3427-36. [PubMed: 17728344]  [MGI Ref ID J:128001]

Dixit R; Zimmer C; Waclaw RR; Mattar P; Shaker T; Kovach C; Logan C; Campbell K; Guillemot F; Schuurmans C. 2011. Ascl1 participates in Cajal-Retzius cell development in the neocortex. Cereb Cortex 21(11):2599-611. [PubMed: 21467208]  [MGI Ref ID J:188692]

Duggan CD; Demaria S; Baudhuin A; Stafford D; Ngai J. 2008. Foxg1 is required for development of the vertebrate olfactory system. J Neurosci 28(20):5229-39. [PubMed: 18480279]  [MGI Ref ID J:136319]

Fode C; Ma Q; Casarosa S; Ang SL; Anderson DJ; Guillemot F. 2000. A role for neural determination genes in specifying the dorsoventral identity of telencephalic neurons. Genes Dev 14(1):67-80. [PubMed: 10640277]  [MGI Ref ID J:59428]

Galichet C; Guillemot F; Parras CM. 2008. Neurogenin 2 has an essential role in development of the dentate gyrus. Development 135(11):2031-41. [PubMed: 18448566]  [MGI Ref ID J:134976]

Ge W; He F; Kim KJ; Blanchi B; Coskun V; Nguyen L; Wu X; Zhao J; Heng JI; Martinowich K; Tao J; Wu H; Castro D; Sobeih MM; Corfas G; Gleeson JG; Greenberg ME; Guillemot F; Sun YE. 2006. Coupling of cell migration with neurogenesis by proneural bHLH factors. Proc Natl Acad Sci U S A 103(5):1319-24. [PubMed: 16432194]  [MGI Ref ID J:106021]

Grimaldi P; Parras C; Guillemot F; Rossi F; Wassef M. 2009. Origins and control of the differentiation of inhibitory interneurons and glia in the cerebellum. Dev Biol 328(2):422-33. [PubMed: 19217896]  [MGI Ref ID J:149261]

Guha A; Vasconcelos M; Cai Y; Yoneda M; Hinds A; Qian J; Li G; Dickel L; Johnson JE; Kimura S; Guo J; McMahon J; McMahon AP; Cardoso WV. 2012. Neuroepithelial body microenvironment is a niche for a distinct subset of Clara-like precursors in the developing airways. Proc Natl Acad Sci U S A 109(31):12592-7. [PubMed: 22797898]  [MGI Ref ID J:188519]

Guillemot F. 1995. Analysis of the role of basic-helix-loop-helix transcription factors in the development of neural lineages in the mouse. Biol Cell 84(1-2):3-6. [PubMed: 8574196]  [MGI Ref ID J:31066]

Guillemot F; Lo LC; Johnson JE; Auerbach A; Anderson DJ; Joyner AL. 1993. Mammalian achaete-scute homolog 1 is required for the early development of olfactory and autonomic neurons. Cell 75(3):463-76. [PubMed: 8221886]  [MGI Ref ID J:15850]

Hatakeyama J; Tomita K; Inoue T; Kageyama R. 2001. Roles of homeobox and bHLH genes in specification of a retinal cell type. Development 128(8):1313-22. [PubMed: 11262232]  [MGI Ref ID J:68135]

Helms AW; Battiste J; Henke RM; Nakada Y; Simplicio N; Guillemot F; Johnson JE. 2005. Sequential roles for Mash1 and Ngn2 in the generation of dorsal spinal cord interneurons. Development 132(12):2709-19. [PubMed: 15901662]  [MGI Ref ID J:98830]

Henke RM; Meredith DM; Borromeo MD; Savage TK; Johnson JE. 2009. Ascl1 and Neurog2 form novel complexes and regulate Delta-like3 (Dll3) expression in the neural tube. Dev Biol 328(2):529-40. [PubMed: 19389376]  [MGI Ref ID J:149457]

Henke RM; Savage TK; Meredith DM; Glasgow SM; Hori K; Dumas J; MacDonald RJ; Johnson JE. 2009. Neurog2 is a direct downstream target of the Ptf1a-Rbpj transcription complex in dorsal spinal cord. Development 136(17):2945-54. [PubMed: 19641016]  [MGI Ref ID J:152184]

Hirsch MR; Tiveron MC; Guillemot F; Brunet JF; Goridis C. 1998. Control of noradrenergic differentiation and Phox2a expression by MASH1 in the central and peripheral nervous system. Development 125(4):599-608. [PubMed: 9435281]  [MGI Ref ID J:46409]

Horton S; Meredith A; Richardson JA; Johnson JE. 1999. Correct coordination of neuronal differentiation events in ventral forebrain requires the bHLH factor MASH1. Mol Cell Neurosci 14(4-5):355-69. [PubMed: 10588390]  [MGI Ref ID J:58160]

Huber K; Bruhl B; Guillemot F; Olson EN; Ernsberger U; Unsicker K. 2002. Development of chromaffin cells depends on MASH1 function. Development 129(20):4729-38. [PubMed: 12361965]  [MGI Ref ID J:79390]

Hufnagel RB; Le TT; Riesenberg AL; Brown NL. 2010. Neurog2 controls the leading edge of neurogenesis in the mammalian retina. Dev Biol 340(2):490-503. [PubMed: 20144606]  [MGI Ref ID J:160260]

Ikeda K; Kageyama R; Suzuki Y; Kawakami K. 2010. Six1 is indispensable for production of functional progenitor cells during olfactory epithelial development. Int J Dev Biol 54(10):1453-64. [PubMed: 21302255]  [MGI Ref ID J:170495]

Ikeda K; Ookawara S; Sato S; Ando Z; Kageyama R; Kawakami K. 2007. Six1 is essential for early neurogenesis in the development of olfactory epithelium. Dev Biol 311(1):53-68. [PubMed: 17880938]  [MGI Ref ID J:126356]

Imayoshi I; Isomura A; Harima Y; Kawaguchi K; Kori H; Miyachi H; Fujiwara T; Ishidate F; Kageyama R. 2013. Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Science 342(6163):1203-8. [PubMed: 24179156]  [MGI Ref ID J:203419]

Ito T; Udaka N; Yazawa T; Okudela K; Hayashi H; Sudo T; Guillemot F; Kageyama R; Kitamura H. 2000. Basic helix-loop-helix transcription factors regulate the neuroendocrine differentiation of fetal mouse pulmonary epithelium Development 127(18):3913-21. [PubMed: 10952889]  [MGI Ref ID J:64088]

Jacob J; Storm R; Castro DS; Milton C; Pla P; Guillemot F; Birchmeier C; Briscoe J. 2009. Insm1 (IA-1) is an essential component of the regulatory network that specifies monoaminergic neuronal phenotypes in the vertebrate hindbrain. Development 136(14):2477-85. [PubMed: 19542360]  [MGI Ref ID J:152424]

Jacob J; Tiveron MC; Brunet JF; Guthrie S. 2000. Role of the target in the pathfinding of facial visceral motor axons. Mol Cell Neurosci 16(1):14-26. [PubMed: 10882479]  [MGI Ref ID J:63720]

Jeong Y; Dolson DK; Waclaw RR; Matise MP; Sussel L; Campbell K; Kaestner KH; Epstein DJ. 2011. Spatial and temporal requirements for sonic hedgehog in the regulation of thalamic interneuron identity. Development 138(3):531-41. [PubMed: 21205797]  [MGI Ref ID J:170540]

Kala K; Haugas M; Lillevali K; Guimera J; Wurst W; Salminen M; Partanen J. 2009. Gata2 is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons. Development 136(2):253-62. [PubMed: 19088086]  [MGI Ref ID J:143527]

Kameda Y. 2005. Mash1 is required for glomus cell formation in the mouse carotid body. Dev Biol 283(1):128-39. [PubMed: 15878769]  [MGI Ref ID J:99328]

Kameda Y; Nishimaki T; Miura M; Jiang SX; Guillemot F. 2007. Mash1 regulates the development of C cells in mouse thyroid glands. Dev Dyn 236(1):262-70. [PubMed: 17103415]  [MGI Ref ID J:116627]

Kokubu H; Ohtsuka T; Kageyama R. 2008. Mash1 is required for neuroendocrine cell development in the glandular stomach. Genes Cells 13(1):41-51. [PubMed: 18173746]  [MGI Ref ID J:138261]

Kriks S; Lanuza GM; Mizuguchi R; Nakafuku M; Goulding M. 2005. Gsh2 is required for the repression of Ngn1 and specification of dorsal interneuron fate in the spinal cord. Development 132(13):2991-3002. [PubMed: 15930101]  [MGI Ref ID J:98802]

Li S; Misra K; Matise MP; Xiang M. 2005. Foxn4 acts synergistically with Mash1 to specify subtype identity of V2 interneurons in the spinal cord. Proc Natl Acad Sci U S A 102(30):10688-93. [PubMed: 16020526]  [MGI Ref ID J:100187]

Lo L; Tiveron MC; Anderson DJ. 1998. MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity. Development 125(4):609-20. [PubMed: 9435282]  [MGI Ref ID J:46371]

Long JE; Cobos I; Potter GB; Rubenstein JL. 2009. Dlx1&2 and Mash1 transcription factors control MGE and CGE patterning and differentiation through parallel and overlapping pathways. Cereb Cortex 19 Suppl 1:i96-106. [PubMed: 19386638]  [MGI Ref ID J:173286]

Long JE; Garel S; Alvarez-Dolado M; Yoshikawa K; Osumi N; Alvarez-Buylla A; Rubenstein JL. 2007. Dlx-dependent and -independent regulation of olfactory bulb interneuron differentiation. J Neurosci 27(12):3230-43. [PubMed: 17376983]  [MGI Ref ID J:119448]

Long JE; Swan C; Liang WS; Cobos I; Potter GB; Rubenstein JL. 2009. Dlx1&2 and Mash1 transcription factors control striatal patterning and differentiation through parallel and overlapping pathways. J Comp Neurol 512(4):556-72. [PubMed: 19030180]  [MGI Ref ID J:173287]

Lopez-Bendito G; Cautinat A; Sanchez JA; Bielle F; Flames N; Garratt AN; Talmage DA; Role LW; Charnay P; Marin O; Garel S. 2006. Tangential neuronal migration controls axon guidance: a role for neuregulin-1 in thalamocortical axon navigation. Cell 125(1):127-42. [PubMed: 16615895]  [MGI Ref ID J:144312]

McNay DE; Pelling M; Claxton S; Guillemot F; Ang SL. 2006. Mash1 is required for generic and subtype differentiation of hypothalamic neuroendocrine cells. Mol Endocrinol 20(7):1623-32. [PubMed: 16469766]  [MGI Ref ID J:110059]

Mizuguchi R; Kriks S; Cordes R; Gossler A; Ma Q; Goulding M. 2006. Ascl1 and Gsh1/2 control inhibitory and excitatory cell fate in spinal sensory interneurons. Nat Neurosci 9(6):770-8. [PubMed: 16715081]  [MGI Ref ID J:110261]

Morikawa Y; Dai YS; Hao J; Bonin C; Hwang S; Cserjesi P. 2005. The basic helix-loop-helix factor Hand 2 regulates autonomic nervous system development. Dev Dyn 234(3):613-21. [PubMed: 16145670]  [MGI Ref ID J:119859]

Morimoto M; Nishinakamura R; Saga Y; Kopan R. 2012. Different assemblies of Notch receptors coordinate the distribution of the major bronchial Clara, ciliated and neuroendocrine cells. Development 139(23):4365-73. [PubMed: 23132245]  [MGI Ref ID J:190886]

Murray RC; Navi D; Fesenko J; Lander AD; Calof AL. 2003. Widespread defects in the primary olfactory pathway caused by loss of Mash1 function. J Neurosci 23(5):1769-80. [PubMed: 12629181]  [MGI Ref ID J:82270]

Nakagawa Y; Johnson JE; O'Leary DD. 1999. Graded and areal expression patterns of regulatory genes and cadherins in embryonic neocortex independent of thalamocortical input. J Neurosci 19(24):10877-85. [PubMed: 10594069]  [MGI Ref ID J:58815]

Nakatani H; Martin E; Hassani H; Clavairoly A; Maire CL; Viadieu A; Kerninon C; Delmasure A; Frah M; Weber M; Nakafuku M; Zalc B; Thomas JL; Guillemot F; Nait-Oumesmar B; Parras C. 2013. Ascl1/Mash1 promotes brain oligodendrogenesis during myelination and remyelination. J Neurosci 33(23):9752-68. [PubMed: 23739972]  [MGI Ref ID J:198643]

Nelson BR; Hartman BH; Ray CA; Hayashi T; Bermingham-McDonogh O; Reh TA. 2009. Acheate-scute like 1 (Ascl1) is required for normal delta-like (Dll) gene expression and notch signaling during retinal development. Dev Dyn 238(9):2163-2178. [PubMed: 19191219]  [MGI Ref ID J:151457]

Nieto M; Schuurmans C; Britz O; Guillemot F. 2001. Neural bHLH genes control the neuronal versus glial fate decision in cortical progenitors. Neuron 29(2):401-13. [PubMed: 11239431]  [MGI Ref ID J:70193]

Niquille M; Garel S; Mann F; Hornung JP; Otsmane B; Chevalley S; Parras C; Guillemot F; Gaspar P; Yanagawa Y; Lebrand C. 2009. Transient neuronal populations are required to guide callosal axons: a role for semaphorin 3C. PLoS Biol 7(10):e1000230. [PubMed: 19859539]  [MGI Ref ID J:154587]

Ohsawa R; Ohtsuka T; Kageyama R. 2005. Mash1 and Math3 are required for development of branchiomotor neurons and maintenance of neural progenitors. J Neurosci 25(25):5857-65. [PubMed: 15976074]  [MGI Ref ID J:99297]

Oishi K; Watatani K; Itoh Y; Okano H; Guillemot F; Nakajima K; Gotoh Y. 2009. Selective induction of neocortical GABAergic neurons by the PDK1-Akt pathway through activation of Mash1. Proc Natl Acad Sci U S A 106(31):13064-9. [PubMed: 19549840]  [MGI Ref ID J:151894]

Pacary E; Heng J; Azzarelli R; Riou P; Castro D; Lebel-Potter M; Parras C; Bell DM; Ridley AJ; Parsons M; Guillemot F. 2011. Proneural transcription factors regulate different steps of cortical neuron migration through Rnd-mediated inhibition of RhoA signaling. Neuron 69(6):1069-84. [PubMed: 21435554]  [MGI Ref ID J:174732]

Parras CM; Galli R; Britz O; Soares S; Galichet C; Battiste J; Johnson JE; Nakafuku M; Vescovi A; Guillemot F. 2004. Mash1 specifies neurons and oligodendrocytes in the postnatal brain. EMBO J 23(22):4495-505. [PubMed: 15496983]  [MGI Ref ID J:94386]

Parras CM; Hunt C; Sugimori M; Nakafuku M; Rowitch D; Guillemot F. 2007. The proneural gene Mash1 specifies an early population of telencephalic oligodendrocytes. J Neurosci 27(16):4233-42. [PubMed: 17442807]  [MGI Ref ID J:121096]

Parras CM; Schuurmans C; Scardigli R; Kim J; Anderson DJ; Guillemot F. 2002. Divergent functions of the proneural genes Mash1 and Ngn2 in the specification of neuronal subtype identity. Genes Dev 16(3):324-38. [PubMed: 11825874]  [MGI Ref ID J:74525]

Pattyn A; Guillemot F; Brunet JF. 2006. Delays in neuronal differentiation in Mash1/Ascl1 mutants. Dev Biol 295(1):67-75. [PubMed: 16677628]  [MGI Ref ID J:110707]

Pattyn A; Simplicio N; van Doorninck JH; Goridis C; Guillemot F; Brunet JF. 2004. Ascl1/Mash1 is required for the development of central serotonergic neurons. Nat Neurosci 7(6):589-95. [PubMed: 15133515]  [MGI Ref ID J:91076]

Peltopuro P; Kala K; Partanen J. 2010. Distinct requirements for Ascl1 in subpopulations of midbrain GABAergic neurons. Dev Biol 343(1-2):63-70. [PubMed: 20417196]  [MGI Ref ID J:162166]

Petryniak MA; Potter GB; Rowitch DH; Rubenstein JL. 2007. Dlx1 and Dlx2 control neuronal versus oligodendroglial cell fate acquisition in the developing forebrain. Neuron 55(3):417-33. [PubMed: 17678855]  [MGI Ref ID J:132162]

Pla P; Hirsch MR; Le Crom S; Reiprich S; Harley VR; Goridis C. 2008. Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors. Neural Dev 3:14. [PubMed: 18565209]  [MGI Ref ID J:160742]

Satow T; Bae SK; Inoue T; Inoue C; Miyoshi G; Tomita K; Bessho Y; Hashimoto N; Kageyama R. 2001. The basic helix-loop-helix gene hesr2 promotes gliogenesis in mouse retina. J Neurosci 21(4):1265-73. [PubMed: 11160397]  [MGI Ref ID J:109366]

Schuurmans C; Armant O; Nieto M; Stenman JM; Britz O; Klenin N; Brown C; Langevin LM; Seibt J; Tang H; Cunningham JM; Dyck R; Walsh C; Campbell K; Polleux F; Guillemot F. 2004. Sequential phases of cortical specification involve Neurogenin-dependent and -independent pathways. EMBO J 23(14):2892-902. [PubMed: 15229646]  [MGI Ref ID J:93057]

Seibt J; Armant O; Le Digarcher A; Castro D; Ramesh V; Journot L; Guillemot F; Vanderhaeghen P; Bouschet T. 2012. Expression at the imprinted dlk1-gtl2 locus is regulated by proneural genes in the developing telencephalon. PLoS One 7(11):e48675. [PubMed: 23139813]  [MGI Ref ID J:195360]

Seta Y; Oda M; Kataoka S; Toyono T; Toyoshima K. 2011. Mash1 is required for the differentiation of AADC-positive type III cells in mouse taste buds. Dev Dyn 240(4):775-84. [PubMed: 21322090]  [MGI Ref ID J:169536]

Sommer L; Shah N; Rao M; Anderson DJ. 1995. The cellular function of MASH1 in autonomic neurogenesis. Neuron 15(6):1245-58. [PubMed: 8845150]  [MGI Ref ID J:30246]

Sugimori M; Nagao M; Parras CM; Nakatani H; Lebel M; Guillemot F; Nakafuku M. 2008. Ascl1 is required for oligodendrocyte development in the spinal cord. Development 135(7):1271-81. [PubMed: 18287202]  [MGI Ref ID J:135661]

Tiveron MC; Pattyn A; Hirsch MR; Brunet JF. 2003. Role of Phox2b and Mash1 in the generation of the vestibular efferent nucleus. Dev Biol 260(1):46-57. [PubMed: 12885554]  [MGI Ref ID J:107718]

Tiveron MC; Rossel M; Moepps B; Zhang YL; Seidenfaden R; Favor J; Konig N; Cremer H. 2006. Molecular interaction between projection neuron precursors and invading interneurons via stromal-derived factor 1 (CXCL12)/CXCR4 signaling in the cortical subventricular zone/intermediate zone. J Neurosci 26(51):13273-8. [PubMed: 17182777]  [MGI Ref ID J:116684]

Tomita K; Moriyoshi K; Nakanishi S; Guillemot F; Kageyama R. 2000. Mammalian achaete-scute and atonal homologs regulate neuronal versus glial fate determination in the central nervous system EMBO J 19(20):5460-72. [PubMed: 11032813]  [MGI Ref ID J:65410]

Torii Ma; Matsuzaki F; Osumi N; Kaibuchi K; Nakamura S; Casarosa S; Guillemot F; Nakafuku M. 1999. Transcription factors Mash-1 and Prox-1 delineate early steps in differentiation of neural stem cells in the developing central nervous system. Development 126(3):443-56. [PubMed: 9876174]  [MGI Ref ID J:53737]

Tucker ES; Lehtinen MK; Maynard T; Zirlinger M; Dulac C; Rawson N; Pevny L; Lamantia AS. 2010. Proliferative and transcriptional identity of distinct classes of neural precursors in the mammalian olfactory epithelium. Development 137(15):2471-81. [PubMed: 20573694]  [MGI Ref ID J:163859]

Tuttle R; Nakagawa Y; Johnson JE; O'Leary DD. 1999. Defects in thalamocortical axon pathfinding correlate with altered cell domains in Mash-1-deficient mice. Development 126(9):1903-16. [PubMed: 10101124]  [MGI Ref ID J:52751]

Ueno T; Ito J; Hoshikawa S; Ohori Y; Fujiwara S; Yamamoto S; Ohtsuka T; Kageyama R; Akai M; Nakamura K; Ogata T. 2012. The identification of transcriptional targets of Ascl1 in oligodendrocyte development. Glia 60(10):1495-505. [PubMed: 22714260]  [MGI Ref ID J:186420]

Virolainen SM; Achim K; Peltopuro P; Salminen M; Partanen J. 2012. Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres. Development 139(20):3795-805. [PubMed: 22991444]  [MGI Ref ID J:188110]

Wang B; Waclaw RR; Allen ZJ 2nd; Guillemot F; Campbell K. 2009. Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon. Neural Dev 4:5. [PubMed: 19208224]  [MGI Ref ID J:147161]

Wildner H; Muller T; Cho SH; Brohl D; Cepko CL; Guillemot F; Birchmeier C. 2006. dILA neurons in the dorsal spinal cord are the product of terminal and non-terminal asymmetric progenitor cell divisions, and require Mash1 for their development. Development 133(11):2105-13. [PubMed: 16690754]  [MGI Ref ID J:109482]

Yun K; Fischman S; Johnson J; De Angelis MH; Weinmaster G; Rubenstein JL. 2002. Modulation of the notch signaling by Mash1 and Dlx1/2 regulates sequential specification and differentiation of progenitor cell types in the subcortical telencephalon. Development 129(21):5029-40. [PubMed: 12397111]  [MGI Ref ID J:79854]

Zimmer C; Tiveron MC; Bodmer R; Cremer H. 2004. Dynamics of Cux2 expression suggests that an early pool of SVZ precursors is fated to become upper cortical layer neurons. Cereb Cortex 14(12):1408-20. [PubMed: 15238450]  [MGI Ref ID J:157752]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

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Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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