Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6JEi-KitlSl-18H    (Changed: 08-APR-08 ) Type Congenic; Radiation Induced Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10

Development
The steel 18 Harwell mutation, then on a mixed C3H and 101 background, was imported in 1988 from Harwell into NCI, Frederick. There, Peter Donovan maintained it by backrossing to C3H/HeN and C3H/HeJ. In 1993 this mutation was imported from NCI to the laboratory of Eva Eicher at The Jackson Laboratory and the estimated backcross generation at that time was greater than N10. The Eicher laboratory then backcrossed this mutation onto a B6 background using both C57BL/6JEi and C57BL/6J.

Related Strains

Strains carrying other alleles of Kitl

000090	129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
008656	B6.Cg-KitlSl-gb/MbeJ
000124	B6.Cg-KitlSl Krt71Ca/J
000160	B6.D2-KitlSl-d/J
000291	C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001380	C3Sn.Cg-KitlSl-con/J
003252	C57BL/6J-KitlSl-20J/J
000979	STOCK KitlSl-16J/J
000161	WB.D2-KitlSl-d/J
000693	WC/ReJ KitlSl/J
100401	WCB6F1/J KitlSl KitlSl-d

View Strains carrying other alleles of Kitl     (11 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Kitl^Sl-18H/Kitl⁺

        involves: 101/H * C3H/HeH

• pigmentation phenotype
• diluted coat color (MGI Ref ID J:18280)
  • greyish coat
• head blaze (MGI Ref ID J:18280)
  • in combination with Kitl^Sl-con there is a prominent white head blaze
• hypopigmentation (MGI Ref ID J:18280)
  • ears and tail are pale
• white spotting (MGI Ref ID J:18280)
  • head spotting and occasional nose and belly spotting
• belly spot (MGI Ref ID J:18280)
  • occasional belly spot
• head spot (MGI Ref ID J:18280)
• lethality-postnatal
• *normal* lethality-postnatal (MGI Ref ID J:18280)
  • small, pale young seen at birth likely comprise the 40% loss of heterozygotes from heterozygous matings
• skin/coat/nails phenotype
• diluted coat color (MGI Ref ID J:18280)
  • greyish coat
• head blaze (MGI Ref ID J:18280)
  • in combination with Kitl^Sl-con there is a prominent white head blaze
• pallor (MGI Ref ID J:18280)
• white spotting (MGI Ref ID J:18280)
  • head spotting and occasional nose and belly spotting
• belly spot (MGI Ref ID J:18280)
  • occasional belly spot
• head spot (MGI Ref ID J:18280)
• hematopoietic system phenotype
• anemia (MGI Ref ID J:18280)
  • pale offspring are presumed to be slightly anemic

Kitl^Sl-18H/Kitl^Sl-18H

        involves: 101/H * C3H/HeH

• lethality-prenatal/perinatal
• embryonic lethality (MGI Ref ID J:18280)
• embryonic lethality before somite formation (MGI Ref ID J:18280)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      urogenital

Reproductive Biology Research

Kitl^Sl-18H related

Cancer Research
Growth Factors/Receptors/Cytokines

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Embryonic Lethality (Homozygous)
Perinatal Lethality
Postnatal Mortality

Hematological Research

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information

Allele Symbol		KitlSl-18H
Allele Name		steel 18 Harwell
Allele Type		Radiation induced
Strain of Origin		(C3H/HeH x 101/H)F1
Gene Symbol and Name		Kitl, kit ligand
Chromosome		10
Gene Common Name(s)		Clo; Con; DKFZp686F2250; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
Molecular Note		This allele comprises a deletion that is cytologically apparent, removing ~2.5% of Chr 10. Southern blot analysis and pulsed field gel electrophoresis showed that this deletion is greater than 810kb. [MGI Ref ID J:10748] [MGI Ref ID J:31566] [MGI Ref ID J:3564]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Cattanach BM; Rasberry C. 1988. A new steel allele with early post-implantation homozygous lethality Mouse News Lett 80:157-58.  [MGI Ref ID J:18280]

Additional References

Kitl^Sl-18H related

Bedell MA; Cleveland LS; O'Sullivan TN; Copeland NG; Jenkins NA. 1996. Deletion and interallelic complementation analysis of Steel mutant mice. Genetics 142(3):935-44. [PubMed: 8849899]  [MGI Ref ID J:31566]

Cattanach BM; Burtenshaw MD; Rasberry C; Evans EP. 1993. Large deletions and other gross forms of chromosome imbalance compatible with viability and fertility in the mouse. Nat Genet 3(1):56-61. [PubMed: 8490655]  [MGI Ref ID J:3564]

Cattanach BM; Raspberry C; Beechey CV. 1995. XY sex reversal associated with autosomal deletions Mouse Genome 93:426.  [MGI Ref ID J:133266]

Copeland NG; Gilbert DJ; Cho BC; Donovan PJ; Jenkins NA; Cosman D; Anderson D; Lyman SD; Williams DE. 1990. Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles. Cell 63(1):175-83. [PubMed: 1698554]  [MGI Ref ID J:10748]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery of Strains Needing Progeny Testing. At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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