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Strain Name:

B6;129S-Apobtm2Sgy Ldlrtm1Her/J

Stock Number:

003000

Availability:

Repository-Cryopreserved

Price and Supply Information

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Genes & Alleles   Apob;   Apobtm2Sgy;   Ldlr;   Ldlrtm1Her;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Donating Investigator Stephen Young,   Gladstone Institutes UCSF
GenerationF?+12p

Strain Description
This strain expresses only APO-B100 (normally expressed in the liver and yolk sac) and is deficient in low density lipoprotein receptor (Ldlrtm1Her/Ldlrtm1Her).

Strain Development
Gene targeting in ES cells can be used to introduce subtle mutations into mice, making it possible to perform "site-directed mutagenesis" in a whole animal. This approach was used to introduce point mutations into the apo-B48-editing codon of the mouse apo-B gene to generate mice that synthesize exclusively one of the two forms of apo-B found in mammals, apo-B48 and apo-B100.

Gene & Allele Details

Allele Symbol Apobtm2Sgy
Allele Name targeted mutation 2, Stephen G Young
Common Name(s) apo-B100;
Mutation Made By Stephen Young,   Gladstone Institutes UCSF
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Apob, apolipoprotein B
Chromosome 12
Gene Common Name(s) AI315052; Aa1064; Ac1-060; ApoB-100; ApoB-48; FLDB; expressed sequence AI315052;
Molecular Note A "hit and run"-type vector was used to create a CTA to TTA mutation in codon 2153 in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB100 isoform is unaffected by this mutation, while no ApoB48 isoform is produced from this allele. [MGI Ref ID J:33830]
 
Allele Symbol Ldlrtm1Her
Allele Name targeted mutation 1, Joachim Herz
Common Name(s) LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her;
Mutation Made By Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Ldlr, low density lipoprotein receptor
Chromosome 9
Gene Common Name(s) FH; FHC;
General Note When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777)
Molecular Note Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394]

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Apobtm2Sgy
Ldlr tm1Her

Colony Maintenance

Breeding & HusbandryThis strain is maintained by homozygous sibling matings. This strain was generated on a 129/SvJae background and is currently on a B6,129 background. The lab regenerates founders every 6 months. Reproduction is good. Homozygous mice are viable and fertile. Coat color expected from breeding:Black
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Apobtm2Sgy allele
002879   B6;129-Apobtm2Sgy Apoetm1Unc/J
002877   B6;129S7-Apobtm2Sgy/J
004192   STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
View Strains carrying   Apobtm2Sgy     (3 strains)

View Strains carrying   Ldlrtm1Her     (11 strains)

Strains carrying other alleles of Apob
002053   B6.129P2-Apobtm1Unc/J
007682   B6.129X1-Apobtm1.1Zc/J
002878   B6;129-Apobtm1Sgy Apoetm1Unc/J
002876   B6;129S-Apobtm1Sgy/J
007683   CByJ.129X1(Cg)-Apobtm1.1Zc/J
007679   SWR.129X1(B6)-Apobtm1.1Zc/J
View Strains carrying other alleles of Apob     (6 strains)

Strains carrying other alleles of Ldlr
005061   C57BL/6J-LdlrHlb301/J
View Strains carrying other alleles of Ldlr     (1 strain)

Research Applications

This mouse can be used to support research in many areas including:

Apobtm2Sgy related

Cardiovascular Research
Atherosclerosis
Hypocholesterolemia
Hypotriglyceridemia

Developmental Biology Research
Neural Tube Defects

Mouse/Human Gene Homologs
hypobetalipoproteinemia, familial

Neurobiology Research
Neural Tube Defects

Ldlrtm1Her related

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia

Metabolism Research
Lipid Metabolism

Mouse/Human Gene Homologs
hypercholesterolemia, familial

References

Selected Reference(s)

Farese RVJr; Veniant MM; Cham CM; Flynn LM; Pierotti V; Loring JF; Traber M; Ruland S; Stokowski RS; Huszar D; Young SG. 1996. Phenotypic analysis of mice expressing exclusively apolipoprotein B48 or apolipoprotein B100. Proc Natl Acad Sci U S A 93(13):6393-8. [PubMed: 8692825]  [MGI Ref ID J:33830]

Additional References

Price and Supply Information

Strain Name: B6;129S-Apobtm2Sgy Ldlrtm1Her/J
Stock Number: 003000

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)
Cryorecovery Fee $2470.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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