Description

Strain Information

Former Names BPH/2@J    (Changed: 15-DEC-04 ) Blood Pressure High    (Changed: 15-DEC-04 ) Type Inbred Strain; Additional information on Inbred Strains. Mating System Sibling x Sibling         (Female x Male) Species laboratory mouse H2 Haplotype d Generation F66+21 (22-JAN-08)

Appearance
albino
Related Genotype: Tyr^c/Tyr^c

Description
The hypertensive BPH/2 mice have elevated systolic blood pressures at five weeks and by 150 days differ from the BPL/1 by 60 mmHg. The BPH/2 strain has a higher heart rate, larger hearts and kidneys, and higher hematocrits than the BPL/1. It also has lower renin, aldosterone and angiotensin I levels compared to the BPL/1 and BPN/3. The original HBP (high blood pressure) and LBP (low blood pressure) selected lines showed a number of biochemical and physiological differences which have not been reexamined in the inbred strains. These include differences in brain catecholamines, calmodulin concentrations, heat sensitivity, alcohol preference, and longevity. The differences in longevity were striking. The hypotensive selected lines lived two to three hundred days longer, on average, than the hypertensive selected line. Biometrical genetic analysis suggested that three to five genes are responsible for the difference in blood pressure between the BPH/2 and BPL/1. A recent genome scan of an F2 from a BPH/2 x BPL/1 cross found three chromosome locations that cosegregated with blood pressure. Two of these sites were verified by candidate gene cosegregation: angiotensinogen on chromosome 8 and mouse kallikrein binding protein on chromosome 12.

Development
In 1964, a two-way selection program was initiated at the Jackson Laboratory to produce hypertensive and hypotensive mice. These colonies were moved to the University of Kansas in 1970, where the selection program continued for a total of 30 generations. The maximum divergence between the two selection lines, HBP and LBP, was 60 mmHg. Brother-sister matings followed the selection program to produce the inbred hypertensive BPH/2 and hypotensive BPL/1 strains. A non-selected control was maintained throughout the selection program; brother-sister matings from this line produced the BPN/3 normotensive strain. These three strains were returned to The Jackson Laboratory in 1997.

Control Information

	Control
	003004 BPN/3J
Considerations for Choosing Controls

Related Strains

Blood Pressure High, Low and Normal Strains

003006	BPL/1J
003004	BPN/3J

View Blood Pressure High, Low and Normal Strains     (2 strains)

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Mouse Phenome Database
Festing Inbred Strain Characteristics: BPH/2

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

cardiovascular system phenotype

hypertension (MGI Ref ID J:106578)

• an increase in kallikrein-like prorenin converting enzyme expression, heightened rennin activity, and diminished angiotensin I activity are found in the submandibular gland of BPH/2J relative to BPN/3J

increased heart rate (MGI Ref ID J:41893)

• BHP/2J mice have a heart rate more than 100 beats per minute faster than BPL/1J and the heart rate of BPN/3J is intermediate compared with these two related strains

increased heart weight (MGI Ref ID J:41893)

• BPN/3J mice have similar heart weight as BPH/2J mice, but increased heart weight compared with BPL/1J

increased systolic blood pressure (MGI Ref ID J:106577)

• elevated systolic blood pressure at 5 months of age in BPH/2J (142 +/- 5 mm Hg) relative to BPN/3J (94 +/- 6 mm Hg)
• higher systolic blood pressure than BPN/3J or BPL/1J mice with 40mm Hg higher than BPL/1J mice at 7 weeks of age and 60mm HG higher by 21 weeks of age

cellular phenotype

oxidative stress (MGI Ref ID J:106577)

• increased malondialdehyde levels in the aorta of BPH/2J relative to BPN/3J and greater release of hydrogen peroxide from the aorta in a 1 hour incubation are found, despite similar SOD1 activity in the aortas of both strains. aortic SOD2 and SOD3 activity
• is increased and aortic catalase activity and protein expression is decreased in BPH/2J relative to BPN/3J
• thiobarbituric acid treatment of homogenized aortas from BPH/2J mice show greater lipid peroxidation than those from BPN/3J

hematopoietic system phenotype

increased hematocrit (MGI Ref ID J:41893)

• BPH/2J mice have a higher hematocrit than BPN/3J or BPL/1J

renal/urinary system phenotype

*normal* renal/urinary system phenotype (MGI Ref ID J:41893)

• BPH/2J have kidney weights that are greater than BPL/1J, but less than BPN/3J

behavior/neurological phenotype

abnormal behavior (MGI Ref ID J:49976)

• strain specific variations in intake of NaCl, KCl, CaCl2, and plain water are found between females of BPL/1J, BPH/2J, and BPN/3J inbred strains, but these differences fail to draw a correlation between hypertension and fluid or salt consumption

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Hypertension

Genes & Alleles

Gene & Allele Information

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Bachmanov AA; Schlager G; Tordoff MG; Beauchamp GK. 1998. Consumption of electrolytes and quinine by mouse strains with different blood pressures. Physiol Behav 64(3):323-30. [PubMed: 9748100]  [MGI Ref ID J:49976]

Schlager G; Sides J. 1997. Characterization of hypertensive and hypotensive inbred strains of mice. Lab Anim Sci 47(3):288-92. [PubMed: 9241632]  [MGI Ref ID J:41893]

Uddin M; Harris-Nelson N. 2004. Renin activity and angiotensin I concentration in genetically selective inbred line of hypertensive mice. Biochem Biophys Res Commun 316(3):842-4. [PubMed: 15033477]  [MGI Ref ID J:89313]

Uddin M; Yang H; Shi M; Polley-Mandal M; Guo Z. 2003. Elevation of oxidative stress in the aorta of genetically hypertensive mice. Mech Ageing Dev 124(7):811-7. [PubMed: 12875744]  [MGI Ref ID J:106577]

Additional References

Schlager G. 1994. Biometrical genetic analysis of blood pressure level in the genetically hypertensive mouse. Clin Exp Hypertens 16(6):809-24. [PubMed: 7858561]  [MGI Ref ID J:21671]

Schlager G. 1974. Selection for blood pressure levels in mice. Genetics 76(3):537-49. [PubMed: 4833575]  [MGI Ref ID J:24609]

Uddin M; Polley-Mandal M; Beg OU. 2003. Kallikrein-like prorenin-converting enzymes in inbred hypertensive mice. Biochem Biophys Res Commun 304(4):724-8. [PubMed: 12727215]  [MGI Ref ID J:106578]

Wong C; Mahapatra NR; Chitbangonsyn S; Mahboubi P; Mahata M; Mahata SK; O'Connor DT. 2003. The angiotensin II receptor (Agtr1a): functional regulatory polymorphisms in a locus genetically linked to blood pressure variation in the mouse. Physiol Genomics 14(1):83-93. [PubMed: 12697907]  [MGI Ref ID J:86190]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Mating System	Sibling x Sibling         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and six weeks of age. This strain is included in the Special Mutant Stock Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	003004 BPN/3J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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