Strain Name:

C.129S1-Il12rb1tm1Jm/J

Stock Number:

003017

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain BALB/c
Donor Strain 129S1 via W9.5 ES (+Kitl-SlJ) ES cell line
 
Donating InvestigatorDr. Jeanne Magram,   Boehringer Ingelheim Pharmaceuticals,Inc

Appearance
albino
Related Genotype: A/A Tyrp1b/Tyrp1b Tyrc/Tyrc

Description
Mice homozygous for the Il12rbtm1Jm targeted mutation are viable and fertile. Con A-activated splenocytes from homozygous mutant mice express only low affinity IL12-binding sites and fail to proliferate or produce interferon gamma in response to IL12. In addition, NK lytic activity in response to IL12 is defective. In vivo immune responses to endotoxin are also impaired.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Il12rb1tm1Jm allele
002984   B6.129S1-Il12rb1tm1Jm/J
View Strains carrying   Il12rb1tm1Jm     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Atypical Mycobacteriosis, Familial   (IL12RB1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Il12rb1tm1Jm/Il12rb1tm1Jm

        involves: 129S1/Sv * C57BL/6J
  • immune system phenotype
  • abnormal lymphocyte physiology
    • Con A-activated splenocytes fail to proliferate in response to IL12 at concentrations as high as 67 nM but proliferate as well as wild-type cells in response to IL2 and IL7   (MGI Ref ID J:42105)
  • decreased circulating interferon-gamma level
    • IFNG is undetectable in sera from recombinant IL12-treated mice and very low in endotoxin LPS-treated mice, unlike in wild-type mice   (MGI Ref ID J:42105)
  • decreased interferon-gamma secretion
    • absence of IL12-stimulated Ifng production in Con A-activated splenocytes   (MGI Ref ID J:42105)
    • splenocytes are profoundly impaired in production of IFNG in response to anti-CD antibody or Con A   (MGI Ref ID J:42105)
  • homeostasis/metabolism phenotype
  • decreased circulating interferon-gamma level
    • IFNG is undetectable in sera from recombinant IL12-treated mice and very low in endotoxin LPS-treated mice, unlike in wild-type mice   (MGI Ref ID J:42105)
  • hematopoietic system phenotype
  • abnormal lymphocyte physiology
    • Con A-activated splenocytes fail to proliferate in response to IL12 at concentrations as high as 67 nM but proliferate as well as wild-type cells in response to IL2 and IL7   (MGI Ref ID J:42105)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il12rb1tm1Jm related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Il12rb1tm1Jm
Allele Name targeted mutation 1, Jeanne Magram
Allele Type Targeted (Null/Knockout)
Common Name(s) IL-12Rbeta1-; IL-12Rbeta1KO;
Mutation Made ByDr. Jeanne Magram,   Boehringer Ingelheim Pharmaceuticals,Inc
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameW9.5/W95
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Il12rb1, interleukin 12 receptor, beta 1
Chromosome 8
Gene Common Name(s) CD212; IL-12R-BETA1; IL-12R[b]; IL12RB;
Molecular Note A neomycin resistance cassette replaced exons 1 to 3 of the gene, deleting the ATG translation initiation codon and causing a frame-shift in the translation reading frame. [MGI Ref ID J:42105]

Genotyping

Genotyping Information

Genotyping Protocols

Il12rb1tm1Jm, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wu C; Ferrante J; Gately MK; Magram J. 1997. Characterization of IL-12 receptor beta1 chain (IL-12Rbeta1)-deficient mice: IL-12Rbeta1 is an essential component of the functional mouse IL-12 receptor. J Immunol 159(4):1658-65. [PubMed: 9257825]  [MGI Ref ID J:42105]

Additional References

Il12rb1tm1Jm related

Afanasyeva M; Wang Y; Kaya Z; Stafford EA; Dohmen KM; Sadighi Akha AA; Rose NR. 2001. Interleukin-12 receptor/STAT4 signaling is required for the development of autoimmune myocarditis in mice by an interferon-gamma-independent pathway. Circulation 104(25):3145-51. [PubMed: 11748115]  [MGI Ref ID J:103357]

Bielinska AU; Makidon PE; Janczak KW; Blanco LP; Swanson B; Smith DM; Pham T; Szabo Z; Kukowska-Latallo JF; Baker JR Jr. 2014. Distinct pathways of humoral and cellular immunity induced with the mucosal administration of a nanoemulsion adjuvant. J Immunol 192(6):2722-33. [PubMed: 24532579]  [MGI Ref ID J:209908]

Brahmachari S; Pahan K. 2009. Suppression of regulatory T cells by IL-12p40 homodimer via nitric oxide. J Immunol 183(3):2045-58. [PubMed: 19587012]  [MGI Ref ID J:151706]

Camoglio L; Juffermans NP; Peppelenbosch M; te Velde AA; ten Kate FJ; van Deventer SJ; Kopf M. 2002. Contrasting roles of IL-12p40 and IL-12p35 in the development of hapten-induced colitis. Eur J Immunol 32(1):261-9. [PubMed: 11782017]  [MGI Ref ID J:73931]

Chang J; Cho JH; Lee SW; Choi SY; Ha SJ; Sung YC. 2004. IL-12 priming during in vitro antigenic stimulation changes properties of CD8 T cells and increases generation of effector and memory cells. J Immunol 172(5):2818-26. [PubMed: 14978082]  [MGI Ref ID J:88236]

Codarri L; Gyulveszi G; Tosevski V; Hesske L; Fontana A; Magnenat L; Suter T; Becher B. 2011. RORgammat drives production of the cytokine GM-CSF in helper T cells, which is essential for the effector phase of autoimmune neuroinflammation. Nat Immunol 12(6):560-7. [PubMed: 21516112]  [MGI Ref ID J:172802]

Coers J; Vance RE; Fontana MF; Dietrich WF. 2007. Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5/Ipaf signalling pathways. Cell Microbiol 9(10):2344-57. [PubMed: 17506816]  [MGI Ref ID J:148669]

Collison LW; Delgoffe GM; Guy CS; Vignali KM; Chaturvedi V; Fairweather D; Satoskar AR; Garcia KC; Hunter CA; Drake CG; Murray PJ; Vignali DA. 2012. The composition and signaling of the IL-35 receptor are unconventional. Nat Immunol 13(3):290-9. [PubMed: 22306691]  [MGI Ref ID J:181328]

Del Nagro CJ; Kolla RV; Rickert RC. 2005. A critical role for complement C3d and the B cell coreceptor (CD19/CD21) complex in the initiation of inflammatory arthritis. J Immunol 175(8):5379-89. [PubMed: 16210644]  [MGI Ref ID J:119107]

Eriksson U; Kurrer MO; Sebald W; Brombacher F; Kopf M. 2001. Dual role of the IL-12/IFN-gamma axis in the development of autoimmune myocarditis: induction by IL-12 and protection by IFN-gamma. J Immunol 167(9):5464-9. [PubMed: 11673566]  [MGI Ref ID J:72665]

Fairweather D; Frisancho-Kiss S; Yusung SA; Barrett MA; Davis SE; Gatewood SJ; Njoku DB; Rose NR. 2004. Interferon-gamma protects against chronic viral myocarditis by reducing mast cell degranulation, fibrosis, and the profibrotic cytokines transforming growth factor-beta 1, interleukin-1 beta, and interleukin-4 in the heart. Am J Pathol 165(6):1883-94. [PubMed: 15579433]  [MGI Ref ID J:94973]

Fairweather D; Yusung S; Frisancho S; Barrett M; Gatewood S; Steele R; Rose NR. 2003. IL-12 Receptor beta1 and Toll-Like Receptor 4 Increase IL-1beta- and IL-18-Associated Myocarditis and Coxsackievirus Replication. J Immunol 170(9):4731-7. [PubMed: 12707353]  [MGI Ref ID J:83020]

Freudenberg MA; Merlin T; Kalis C; Chvatchko Y; Stubig H; Galanos C. 2002. Cutting edge: a murine, IL-12-independent pathway of IFN-gamma induction by gram-negative bacteria based on STAT4 activation by Type I IFN and IL-18 signaling. J Immunol 169(4):1665-8. [PubMed: 12165484]  [MGI Ref ID J:120706]

Gyulveszi G; Haak S; Becher B. 2009. IL-23-driven encephalo-tropism and Th17 polarization during CNS-inflammation in vivo. Eur J Immunol 39(7):1864-1869. [PubMed: 19544494]  [MGI Ref ID J:150273]

Jana M; Mondal S; Jana A; Pahan K. 2014. Interleukin-12 (IL-12), but not IL-23, induces the expression of IL-7 in microglia and macrophages: implications for multiple sclerosis. Immunology 141(4):549-63. [PubMed: 24224652]  [MGI Ref ID J:211495]

Kano S; Sato K; Morishita Y; Vollstedt S; Kim S; Bishop K; Honda K; Kubo M; Taniguchi T. 2008. The contribution of transcription factor IRF1 to the interferon-gamma-interleukin 12 signaling axis and TH1 versus TH-17 differentiation of CD4+ T cells. Nat Immunol 9(1):34-41. [PubMed: 18059273]  [MGI Ref ID J:130476]

Kim DJ; Youn JI; Seo SH; Jin HT; Sung YC. 2008. Differential regulation of antigen-specific CD8+ T cell responses by IL-12p40 in a dose-dependent manner. J Immunol 180(11):7167-74. [PubMed: 18490715]  [MGI Ref ID J:136348]

Kurokawa M; Konno S; Takahashi A; Plunkett B; Rittling SR; Matsui Y; Kon S; Morimoto J; Uede T; Matsukura S; Kokubu F; Adachi M; Nishimura M; Huang SK. 2009. Regulatory role of DC-derived osteopontin in systemic allergen sensitization. Eur J Immunol 39(12):3323-30. [PubMed: 19830723]  [MGI Ref ID J:155466]

Langowski JL; Zhang X; Wu L; Mattson JD; Chen T; Smith K; Basham B; McClanahan T; Kastelein RA; Oft M. 2006. IL-23 promotes tumour incidence and growth. Nature 442(7101):461-5. [PubMed: 16688182]  [MGI Ref ID J:111568]

Lochner M; Peduto L; Cherrier M; Sawa S; Langa F; Varona R; Riethmacher D; Si-Tahar M; Di Santo JP; Eberl G. 2008. In vivo equilibrium of proinflammatory IL-17+ and regulatory IL-10+ Foxp3+ RORgamma t+ T cells. J Exp Med 205(6):1381-93. [PubMed: 18504307]  [MGI Ref ID J:137020]

Miller HE; Robinson RT. 2012. Early Control of Mycobacterium tuberculosis Infection Requires il12rb1 Expression by rag1-Dependent Lineages. Infect Immun 80(11):3828-41. [PubMed: 22907814]  [MGI Ref ID J:187984]

Oh JZ; Kurche JS; Burchill MA; Kedl RM. 2011. TLR7 enables cross-presentation by multiple dendritic cell subsets through a type I IFN-dependent pathway. Blood 118(11):3028-38. [PubMed: 21813451]  [MGI Ref ID J:177064]

Piccotti JR; Li K; Chan SY; Eichwald EJ; Bishop DK. 1999. Interleukin-12 (IL-12)-driven alloimmune responses in vitro and in vivo: requirement for beta1 subunit of the IL-12 receptor. Transplantation 67(11):1453-60. [PubMed: 10385085]  [MGI Ref ID J:55696]

Rangachari M; Mauermann N; Marty RR; Dirnhofer S; Kurrer MO; Komnenovic V; Penninger JM; Eriksson U. 2006. T-bet negatively regulates autoimmune myocarditis by suppressing local production of interleukin 17. J Exp Med 203(8):2009-19. [PubMed: 16880257]  [MGI Ref ID J:124393]

Richer MJ; Nolz JC; Harty JT. 2013. Pathogen-specific inflammatory milieux tune the antigen sensitivity of CD8(+) T cells by enhancing T cell receptor signaling. Immunity 38(1):140-52. [PubMed: 23260194]  [MGI Ref ID J:192617]

Robinson RT; Khader SA; Martino CA; Fountain JJ; Teixeira-Coelho M; Pearl JE; Smiley ST; Winslow GM; Woodland DL; Walter MJ; Conejo-Garcia JR; Gubler U; Cooper AM. 2010. Mycobacterium tuberculosis infection induces il12rb1 splicing to generate a novel IL-12Rbeta1 isoform that enhances DC migration. J Exp Med 207(3):591-605. [PubMed: 20212068]  [MGI Ref ID J:158809]

Rowe JH; Ertelt JM; Way SS. 2012. Innate IFN-gamma is essential for programmed death ligand-1-mediated T cell stimulation following Listeria monocytogenes infection. J Immunol 189(2):876-84. [PubMed: 22711893]  [MGI Ref ID J:189796]

Russell TD; Yan Q; Fan G; Khalifah AP; Bishop DK; Brody SL; Walter MJ. 2003. IL-12 p40 homodimer-dependent macrophage chemotaxis and respiratory viral inflammation are mediated through IL-12 receptor beta1. J Immunol 171(12):6866-74. [PubMed: 14662893]  [MGI Ref ID J:86929]

Sandau MM; Winstead CJ; Jameson SC. 2007. IL-15 is required for sustained lymphopenia-driven proliferation and accumulation of CD8 T cells. J Immunol 179(1):120-5. [PubMed: 17579029]  [MGI Ref ID J:149423]

Satoh-Takayama N; Vosshenrich CA; Lesjean-Pottier S; Sawa S; Lochner M; Rattis F; Mention JJ; Thiam K; Cerf-Bensussan N; Mandelboim O; Eberl G; Di Santo JP. 2008. Microbial flora drives interleukin 22 production in intestinal NKp46+ cells that provide innate mucosal immune defense. Immunity 29(6):958-70. [PubMed: 19084435]  [MGI Ref ID J:142654]

Slutter B; Pewe LL; Kaech SM; Harty JT. 2013. Lung airway-surveilling CXCR3(hi) memory CD8(+) T cells are critical for protection against influenza A virus. Immunity 39(5):939-48. [PubMed: 24238342]  [MGI Ref ID J:208985]

Vom Berg J; Prokop S; Miller KR; Obst J; Kalin RE; Lopategui-Cabezas I; Wegner A; Mair F; Schipke CG; Peters O; Winter Y; Becher B; Heppner FL. 2012. Inhibition of IL-12/IL-23 signaling reduces Alzheimer's disease-like pathology and cognitive decline. Nat Med 18(12):1812-9. [PubMed: 23178247]  [MGI Ref ID J:194214]

Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566]  [MGI Ref ID J:126126]

Zhang GX; Gran B; Yu S; Li J; Siglienti I; Chen X; Kamoun M; Rostami A. 2003. Induction of experimental autoimmune encephalomyelitis in IL-12 receptor-beta 2-deficient mice: IL-12 responsiveness is not required in the pathogenesis of inflammatory demyelination in the central nervous system. J Immunol 170(4):2153-60. [PubMed: 12574388]  [MGI Ref ID J:126707]

Zhang GX; Yu S; Gran B; Li J; Siglienti I; Chen X; Calida D; Ventura E; Kamoun M; Rostami A. 2003. Role of IL-12 receptor beta1 in regulation of T cell response by APC in experimental autoimmune encephalomyelitis. J Immunol 171(9):4485-92. [PubMed: 14568921]  [MGI Ref ID J:86184]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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