Description

Strain Information

Type Recombinant Congenic (Rc); Additional information on Recombinant Congenic Mice. Species laboratory mouse Background Strain CBA/JLsLt Donor Strain NOD/Shi H2 Haplotype k Generation F25+33p (14-NOV-04)   Donating Investigator Edward Leiter,   The Jackson Laboratory

Appearance
agouti
Related Genotype: A/A

Description
Neither spontaneous IDDM nor significant insulitis has been observed in any of the NOcCB or CBcNO recombinant congenic strains. Histologic analysis revealed leukocyte infiltration of the submandibular salivary glands (sialitis) of aging mice. Mice of these strains also developed pancreatic infiltrates, but these were limited to the perivascular and periductal regions of the pancreas (peri-insulitis) and did not focus on the islets. All strains exhibited strong resistance to induction of both IDDM and insulitis by a cyclophosphamide treatment regimen that causes diabetes within two weeks of the second dose in 90% of female NOD mice.

Development
The CBcNO6 recombinant congenic strain was derived from an initial cross between mice of strains CBA/JLsLt and NOD/Shi. The former is "a substrain of CBA/J selected for high incidence of spontaneous...pancreatic necrosis." An F1 female from this mating was backcrossed to a CBA/JLsLt male, and the resultant N2F1 animals were sib-mated. "At F13...CBcNO6 was split into two separate lines, with the new subline designated CBcNO7....At F20, preliminary genome scan analysis of CBcNO7 mice...revealed a small amount of residual heterozygosity on Chrs 2 and 3, permitting selection of four sublines (CBcNO7A, B, C and D) based upon their genotype at D2Mit77 (a marker for the Idd13 region in NOD) and D3Mit100 (a marker for the Idd10/Idd17 region in NOD)....Residual heterozygosity at other loci...was fixed to homozygosity in random fashion." CBcNO7C/Lt is homozygous for the CBA alleles at D2Mit77, on Chr 2, and the Chr 3 loci D3Mit22, Fcgr1 and Amy1.

Control Information

		NOD/LtJ (001976) and CBA/J (000656) may be used as donor and host strain controls, respectively; the latter is only an approximate control, as it is not of the subline employed in constructing these recombinant congenic strains.
Considerations for Choosing Controls

Related Strains

CBcNO Strains

002349	CBcNO6/LtJ
003052	CBcNO7A/LtJ
003053	CBcNO7B/LtJ
003055	CBcNO7D/LtJ

View CBcNO Strains     (4 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (Recombinant Congenic Strains)

Genes & Alleles

Gene & Allele Information

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Reifsnyder PC; Flynn JC; Gavin AL; Simone EA; Sharp JJ; Herberg L ; Leiter EH. 1999. Genotypic and phenotypic characterization of six new recombinant congenic strains derived from NOD/Shi and CBA/J genomes. Mamm Genome 10(2):161-7. [PubMed: 9922397]  [MGI Ref ID J:52864]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

		NOD/LtJ (001976) and CBA/J (000656) may be used as donor and host strain controls, respectively; the latter is only an approximate control, as it is not of the subline employed in constructing these recombinant congenic strains.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.