Strain Name:

B6.NOD-(D3Mit132-Tshb)/J

Stock Number:

003059

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Availability:

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.NOD-c3    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain NOD/Uf
H2 Haplotypeb
 
Donating Investigator Wakeland,  

Appearance
black
Related Genotype: a/a

Description
This strain is congenic for a 43 cM segment of chromosome 3 extending from D3Mit132through Tshb(thyroid stimulating hormone, beta subunit) that includes the diabetes susceptibility loci Idd3and Idd10 (which has been subsequently refined into three diabetes susceptibility loci: Idd10, Idd17, and Idd18). The name given this segment in the primary reference is c3. Upon histologic examination of the pancreas, B6.NOD-(D3Mit132-Tshb) female mice exhibited somewhat higher incidence of periinsulitis (perivascular, periductal and/or periislet infiltration by mononuclear cells) than did C57BL/6J controls, but the difference was not statistically significant. Female B6.NOD-(D3Mit132-Tshb) mice had twice the incidence of periinsulitis as males; female NOD mice are more susceptible than males to both insulitis and diabetes.

Development
Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred in the laboratory of E.K. Wakeland from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate mice homozygous for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest. B6.NOD-(D3Mit132-Tshb) includes NOD alleles for both Chr 3 loci associated with insulitis and diabetes: Idd3,mapping near Il2;and Idd10,mapping near Tshb.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Idd10NOD/ShiLt allele
003067   B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J
View Strains carrying   Idd10NOD/ShiLt     (1 strain)

Strains carrying   Idd3NOD allele
003067   B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J
View Strains carrying   Idd3NOD     (1 strain)

Strains carrying other alleles of D3Mit132
003067   B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J
View Strains carrying other alleles of D3Mit132     (1 strain)

View Strains carrying other alleles of Idd10     (8 strains)

View Strains carrying other alleles of Idd3     (10 strains)

Strains carrying other alleles of Tshb
003067   B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J
003857   NOD.B6(PL)-(D3Mit267-D3Mit75)/Lt
View Strains carrying other alleles of Tshb     (2 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Inflammation

Idd10NOD/ShiLt related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
      NOD/ShiLtJ MHC Congenics

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Idd3NOD related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
      NOD/ShiLtJ MHC Congenics

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information provided by MGI

 
Gene Symbol and Name D3Mit132, DNA segment, Chr 3, Massachusetts Institute of Technology 132
Chromosome 3
 
Gene Symbol and Name Tshb, thyroid stimulating hormone, beta subunit
Chromosome 3
Gene Common Name(s) TSH-B; TSH-BETA; thyrotropin;
 
Allele Symbol Idd10NOD/ShiLt
Allele Name NOD/ShiLt
Allele Type QTL
Strain of OriginNOD/ShiLt
Gene Symbol and Name Idd10, insulin dependent diabetes susceptibility 10
Chromosome 3
Gene Common Name(s) Idd-10;
 
Allele Symbol Idd3NOD
Allele Name NOD
Allele Type QTL
Strain of OriginNOD
Gene Symbol and Name Idd3, insulin dependent diabetes susceptibility 3
Chromosome 3
Gene Common Name(s) Idd-3;
General Note Idd3 is also associated with insulitis.

This allele (on a C57BL/6 background) also confers defective cytokine response (IL-2, IL-4, IFN-gamma, CSF-2) when stimulated with antigens (J:85823).

Il2 is a proposed candidate gene for Idd3. The electrophoretic mobility pattern of IL-2 correlates with type 1 diabetes incidence. Resistant strain C57BL/6J displays a heterogeneous IL-2 mobility pattern with major protein species ranging from 17-19 kDa while susceptible strains 129 and NOD display a homogeneous IL-2 mobility pattern with one major protein species at approximately 21-22 kDa.

Molecular Note This allele confers susceptibility to insulin dependent diabetes compared to C57BL/10. This allele confers decreased Ctla4 expression in activated T-cells compared to C57BL/6. [MGI Ref ID J:106844] [MGI Ref ID J:3351]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Lyons PA; Armitage N; Argentina F; Denny P; Hill NJ; Lord CJ; Wilusz MB; Peterson LB; Wicker LS; Todd JA. 2000. Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping. Genome Res 10(4):446-53. [PubMed: 10779485]  [MGI Ref ID J:61763]

Podolin PL; Denny P; Armitage N; Lord CJ; Hill NJ; Levy ER; Peterson LB ; Todd JA ; Wicker LS ; Lyons PA. 1998. Localization of two insulin-dependent diabetes (Idd) genes to the Idd10 region on mouse chromosome 3. Mamm Genome 9(4):283-6. [PubMed: 9530623]  [MGI Ref ID J:46659]

Yui MA; Muralidharan K; Moreno-Altamirano B; Perrin G; Chestnut K; Wakeland EK. 1996. Production of congenic mouse strains carrying NOD-derived diabetogenic genetic intervals: an approach for the genetic dissection of complex traits. Mamm Genome 7(5):331-4. [PubMed: 8661724]  [MGI Ref ID J:33172]

Idd10NOD/ShiLt related

Ghosh S; Palmer SM; Rodrigues NR; Cordell HJ; Hearne CM; Cornall RJ; Prins JB; McShane P; Lathrop GM; Peterson LB; Wicker LS; Todd JA. 1993. Polygenic control of autoimmune diabetes in nonobese diabetic mice. Nat Genet 4(4):404-9. [PubMed: 8401590]  [MGI Ref ID J:13557]

Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418]  [MGI Ref ID J:85823]

Mcaleer MA; Reifsnyder P; Palmer SM; Prochazka M; Love JM; Copeman JB; Powell EE; Rodrigues NR; Prins JB; Serreze DV; Delarato NH; Wicker LS; Peterson LB; Schork NJ; Todd JA; Leiter EH. 1995. Crosses of NOD mice with the related NON strain: A polygenic model for IDDM. Diabetes 44(10):1186-1195. [PubMed: 7556956]  [MGI Ref ID J:28947]

Podolin PL; Denny P; Armitage N; Lord CJ; Hill NJ; Levy ER; Peterson LB ; Todd JA ; Wicker LS ; Lyons PA. 1998. Localization of two insulin-dependent diabetes (Idd) genes to the Idd10 region on mouse chromosome 3. Mamm Genome 9(4):283-6. [PubMed: 9530623]  [MGI Ref ID J:46659]

Podolin PL; Denny P; Lord CJ; Hill NJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 1997. Congenic mapping of the insulin-dependent diabetes (Idd) gene, Idd10, localizes two gene mediating the Idd10 effect and eliminates the candidate Fcgr1. J Immunol 159(4):1835-1843. [PubMed: 9257847]  [MGI Ref ID J:42103]

Idd3NOD related

Brayer J; Lowry J; Cha S; Robinson CP; Yamachika S; Peck AB; Humphreys-Beher MG. 2000. Alleles from chromosomes 1 and 3 of NOD mice combine to influence Sjogren's syndrome-like autoimmune exocrinopathy. J Rheumatol 27(8):1896-904. [PubMed: 10955330]  [MGI Ref ID J:71276]

Cornall RJ. 1993. Genetics of a multifactorial disease: autoimmune type 1 diabetes mellitus. Clin Sci (Colch) 84(3):257-62. [PubMed: 8384947]  [MGI Ref ID J:17906]

Encinas JA; Wicker LS; Peterson LB; Mukasa A; Teuscher C; Sobel R; Weiner HL; Seidman CE; Seidman JG; Kuchroo VK. 1999. QTL influencing autoimmune diabetes and encephalomyelitis map to a 0.15-cM region containing Il2. Nat Genet 21(2):158-60. [PubMed: 9988264]  [MGI Ref ID J:52572]

Lundholm M; Mayans S; Motta V; Lofgren-Burstrom A; Danska J; Holmberg D. 2010. Variation in the Cd3zeta (Cd247) gene correlates with altered T cell activation and is associated with autoimmune diabetes. J Immunol 184(10):5537-44. [PubMed: 20400699]  [MGI Ref ID J:160996]

Lundholm M; Motta V; Lofgren-Burstrom A; Duarte N; Bergman ML; Mayans S; Holmberg D. 2006. Defective induction of CTLA-4 in the NOD mouse is controlled by the NOD allele of Idd3/IL-2 and a novel locus (Ctex) telomeric on chromosome 1. Diabetes 55(2):538-44. [PubMed: 16443792]  [MGI Ref ID J:106844]

Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418]  [MGI Ref ID J:85823]

Nguyen CQ; Kim H; Cornelius JG; Peck AB. 2007. Development of Sjogren's syndrome in nonobese diabetic-derived autoimmune-prone C57BL/6.NOD-Aec1Aec2 mice is dependent on complement component-3. J Immunol 179(4):2318-29. [PubMed: 17675493]  [MGI Ref ID J:151221]

Nguyen CQ; Sharma A; She JX; McIndoe RA; Peck AB. 2009. Differential gene expressions in the lacrimal gland during development and onset of keratoconjunctivitis sicca in Sjogren's syndrome (SJS)-like disease of the C57BL/6.NOD-Aec1Aec2 mouse. Exp Eye Res 88(3):398-409. [PubMed: 19103199]  [MGI Ref ID J:146559]

Nguyen CQ; Yin H; Lee BH; Chiorini JA; Peck AB. 2011. IL17: potential therapeutic target in Sjogren's syndrome using adenovirus-mediated gene transfer. Lab Invest 91(1):54-62. [PubMed: 20856230]  [MGI Ref ID J:167186]

Podolin PL; Denny P; Lord CJ; Hill NJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 1997. Congenic mapping of the insulin-dependent diabetes (Idd) gene, Idd10, localizes two gene mediating the Idd10 effect and eliminates the candidate Fcgr1. J Immunol 159(4):1835-1843. [PubMed: 9257847]  [MGI Ref ID J:42103]

Podolin PL; Wilusz MB; Cubbon RM; Pajvani U; Lord CJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 2000. Differential glycosylation of interleukin 2, the molecular basis for the NOD Idd3 type 1 diabetes gene? Cytokine 12(5):477-82. [PubMed: 10857762]  [MGI Ref ID J:108210]

Todd JA; Aitman TJ; Cornall RJ; Ghosh S; Hall JR; Hearne CM; Knight AM; Love JM; McAleer MA; Prins JB; Rodrigues N; Lathrop M; Pressey A; DeLarato NH; Peterson LB; Wicker LS. 1991. Genetic analysis of autoimmune type 1 diabetes mellitus in mice [see comments] Nature 351(6327):542-7. [PubMed: 1675432]  [MGI Ref ID J:3351]

Wilson EB; Livingstone AM. 2008. Cutting edge: CD4+ T cell-derived IL-2 is essential for help-dependent primary CD8+ T cell responses. J Immunol 181(11):7445-8. [PubMed: 19017930]  [MGI Ref ID J:142211]

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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