Description

Strain Information

Former Names B6.NOD-c3    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6 Donor Strain NOD/Uf H2 Haplotype b Generation N7F24p   Donating Investigator Wakeland,

Appearance
black
Related Genotype: a/a

Description
This strain is congenic for a 43 cM segment of chromosome 3 extending from D3Mit132through Tshb(thyroid stimulating hormone, beta subunit) that includes the diabetes susceptibility loci Idd3and Idd10 (which has been subsequently refined into three diabetes susceptibility loci: Idd10, Idd17, and Idd18). The name given this segment in the primary reference is c3. Upon histologic examination of the pancreas, B6.NOD-(D3Mit132-Tshb) female mice exhibited somewhat higher incidence of periinsulitis (perivascular, periductal and/or periislet infiltration by mononuclear cells) than did C57BL/6J controls, but the difference was not statistically significant. Female B6.NOD-(D3Mit132-Tshb) mice had twice the incidence of periinsulitis as males; female NOD mice are more susceptible than males to both insulitis and diabetes.

Development
Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred in the laboratory of E.K. Wakeland from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate mice homozygous for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest. B6.NOD-(D3Mit132-Tshb) includes NOD alleles for both Chr 3 loci associated with insulitis and diabetes: Idd3,mapping near Il2;and Idd10,mapping near Tshb.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Idd10^NOD/ShiLt allele

003067

B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J

View Strains carrying   Idd10^NOD/ShiLt     (1 strain)

Strains carrying   Idd3^NOD allele

003067

B6.NOD-(D3Mit132-Tshb) (D17Mit21-D17Mit10)/J

View Strains carrying   Idd3^NOD     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Inflammation

Idd10^NOD/ShiLt related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ MHC Congenics)

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Idd3^NOD related

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ MHC Congenics)

Immunology and Inflammation Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Gene Symbol and Name		Tshb, thyroid stimulating hormone, beta subunit
Chromosome		3
Gene Common Name(s)		CHNG4; TSH-BETA; thyrotropin;
Gene Symbol and Name		D3Mit132, DNA segment, Chr 3, Massachusetts Institute of Technology 132
Chromosome		3
Allele Symbol		Idd10NOD/ShiLt
Allele Name		NOD/ShiLt
Allele Type		QTL
Strain of Origin		NOD/ShiLt
Gene Symbol and Name		Idd10, insulin dependent diabetes susceptibility 10
Chromosome		3
Gene Common Name(s)		Idd-10;
Allele Symbol		Idd3NOD
Allele Name		NOD
Allele Type		QTL
Strain of Origin		NOD
Gene Symbol and Name		Idd3, insulin dependent diabetes susceptibility 3
Chromosome		3
Gene Common Name(s)		Idd-3;
General Note		Idd3 is also associated with insulitis. This allele (on a C57BL/6 background) also confers defective cytokine response (IL-2, IL-4, IFN-gamma, CSF-2) when stimulated with antigens (J:85823). Il2 is a proposed candidate gene for Idd3. The electrophoretic mobility pattern of IL-2 correlates with type 1 diabetes incidence. Resistant strain C57BL/6J displays a heterogenous IL-2 mobility pattern with major protein species ranging from 17-19 kDa while susceptible strains 129 and NOD display a homogenous IL-2 mobility pattern with one major protein species at approximately 21-22 kDa.
Molecular Note		This allele confers susceptibility to insulin dependent diabetes compared to C57BL/10. This allele confers decreased Ctla4 expression in activated T-cells compared to C57BL/6. [MGI Ref ID J:106844] [MGI Ref ID J:3351]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Lyons PA; Armitage N; Argentina F; Denny P; Hill NJ; Lord CJ; Wilusz MB; Peterson LB; Wicker LS; Todd JA. 2000. Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping. Genome Res 10(4):446-53. [PubMed: 10779485]  [MGI Ref ID J:61763]

Podolin PL; Denny P; Armitage N; Lord CJ; Hill NJ; Levy ER; Peterson LB ; Todd JA ; Wicker LS ; Lyons PA. 1998. Localization of two insulin-dependent diabetes (Idd) genes to the Idd10 region on mouse chromosome 3. Mamm Genome 9(4):283-6. [PubMed: 9530623]  [MGI Ref ID J:46659]

Yui MA; Muralidharan K; Moreno-Altamirano B; Perrin G; Chestnut K; Wakeland EK. 1996. Production of congenic mouse strains carrying NOD-derived diabetogenic genetic intervals: an approach for the genetic dissection of complex traits. Mamm Genome 7(5):331-4. [PubMed: 8661724]  [MGI Ref ID J:33172]

Idd10^NOD/ShiLt related

Ghosh S; Palmer SM; Rodrigues NR; Cordell HJ; Hearne CM; Cornall RJ; Prins JB; McShane P; Lathrop GM; Peterson LB; Wicker LS; Todd JA. 1993. Polygenic control of autoimmune diabetes in nonobese diabetic mice. Nat Genet 4(4):404-9. [PubMed: 8401590]  [MGI Ref ID J:13557]

Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418]  [MGI Ref ID J:85823]

Mcaleer MA; Reifsnyder P; Palmer SM; Prochazka M; Love JM; Copeman JB; Powell EE; Rodrigues NR; Prins JB; Serreze DV; Delarato NH; Wicker LS; Peterson LB; Schork NJ; Todd JA; Leiter EH. 1995. Crosses of NOD mice with the related NON strain: A polygenic model for IDDM. Diabetes 44(10):1186-1195. [PubMed: 7556956]  [MGI Ref ID J:28947]

Podolin PL; Denny P; Armitage N; Lord CJ; Hill NJ; Levy ER; Peterson LB ; Todd JA ; Wicker LS ; Lyons PA. 1998. Localization of two insulin-dependent diabetes (Idd) genes to the Idd10 region on mouse chromosome 3. Mamm Genome 9(4):283-6. [PubMed: 9530623]  [MGI Ref ID J:46659]

Podolin PL; Denny P; Lord CJ; Hill NJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 1997. Congenic mapping of the insulin-dependent diabetes (Idd) gene, Idd10, localizes two gene mediating the Idd10 effect and eliminates the candidate Fcgr1. J Immunol 159(4):1835-1843. [PubMed: 9257847]  [MGI Ref ID J:42103]

Idd3^NOD related

Brayer J; Lowry J; Cha S; Robinson CP; Yamachika S; Peck AB; Humphreys-Beher MG. 2000. Alleles from chromosomes 1 and 3 of NOD mice combine to influence Sjogren's syndrome-like autoimmune exocrinopathy. J Rheumatol 27(8):1896-904. [PubMed: 10955330]  [MGI Ref ID J:71276]

Cornall RJ. 1993. Genetics of a multifactorial disease: autoimmune type 1 diabetes mellitus. Clin Sci (Colch) 84(3):257-62. [PubMed: 8384947]  [MGI Ref ID J:17906]

Encinas JA; Wicker LS; Peterson LB; Mukasa A; Teuscher C; Sobel R; Weiner HL; Seidman CE; Seidman JG; Kuchroo VK. 1999. QTL influencing autoimmune diabetes and encephalomyelitis map to a 0.15-cM region containing Il2. Nat Genet 21(2):158-60. [PubMed: 9988264]  [MGI Ref ID J:52572]

Lundholm M; Motta V; Lofgren-Burstrom A; Duarte N; Bergman ML; Mayans S; Holmberg D. 2006. Defective induction of CTLA-4 in the NOD mouse is controlled by the NOD allele of Idd3/IL-2 and a novel locus (Ctex) telomeric on chromosome 1. Diabetes 55(2):538-44. [PubMed: 16443792]  [MGI Ref ID J:106844]

Matsuki N; Stanic AK; Embers ME; Van Kaer L; Morel L; Joyce S. 2003. Genetic dissection of V alpha 14J alpha 18 natural T cell number and function in autoimmune-prone mice. J Immunol 170(11):5429-37. [PubMed: 12759418]  [MGI Ref ID J:85823]

Podolin PL; Denny P; Lord CJ; Hill NJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 1997. Congenic mapping of the insulin-dependent diabetes (Idd) gene, Idd10, localizes two gene mediating the Idd10 effect and eliminates the candidate Fcgr1. J Immunol 159(4):1835-1843. [PubMed: 9257847]  [MGI Ref ID J:42103]

Podolin PL; Wilusz MB; Cubbon RM; Pajvani U; Lord CJ; Todd JA; Peterson LB; Wicker LS; Lyons PA. 2000. Differential glycosylation of interleukin 2, the molecular basis for the NOD Idd3 type 1 diabetes gene? Cytokine 12(5):477-82. [PubMed: 10857762]  [MGI Ref ID J:108210]

Todd JA; Aitman TJ; Cornall RJ; Ghosh S; Hall JR; Hearne CM; Knight AM; Love JM; McAleer MA; Prins JB; Rodrigues N; Lathrop M; Pressey A; DeLarato NH; Peterson LB; Wicker LS. 1991. Genetic analysis of autoimmune type 1 diabetes mellitus in mice [see comments] Nature 351(6327):542-7. [PubMed: 1675432]  [MGI Ref ID J:3351]

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37. [PubMed: 17277778]  [MGI Ref ID J:120349]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
fax:	207-288-6655

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