Strain Name: |
NOD/ShiLt-Tg(Ins2-GAD2)1Lt/LtJ |
|---|---|
Stock Number: |
003074 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD/Lt-Tg(Ins2-GAD2)1Lt/LtJ (Changed: 23-FEB-07
) |
|
NOD/Lt-Tg(Ins2-GAD2)1Lt/Lt (Changed: 17-JAN-07
) | |
|
NOD/Lt-Tg(GAD2)1Lt (Changed: 21-JUL-06
) | |
|
GAD65;YLin (Changed: 15-DEC-04
) | |
| Genes & Alleles | GAD2; Ins2; Tg(Ins2-GAD2)1Lt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Edward Leiter, The Jackson Laboratory H2 Haplotype g7 Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Transgenic mice are characterized by pancreatic beta cells that express a rat insulin promoter 7, Ins2, regulated transgene encoding a human B-cell autoantigen, GAD2. These mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. This NOD/Lt transgenic demonstrates a Y Chr. integration-site as only male progeny are transgenic. Expression of the transgene in the islets has been confirmed by RT-PCR and Western blot analysis. Southern analysis confirms 17 copies of the transgene are present, compared to the A-line that has 4-5 copies (Stock No. 5870). Elevated levels of GAD2 expression in transgenic B-cells do not influence non-fasting plasma levels of insulin and glucagon. At 50 weeks of age 55% of the transgenic males were diabetic, this is not significantly different from NOD/Lt male cohorts. Insulitis in 9 week old females is not significantly different between transgenics and controls.This model provides a tool for studying at the role of autoantigens, specifically GAD2, in diabetes.
Strain Development
Glutamic acid decarboxylase 2, GAD2 (commonly referred to as GAD65) and located on Chr. 2, is highly expressed in human and rat beta cells, but little to no expression in mouse beta cells. Because GAD2 is a major candidate autoantigen in human T1D, a human GAD2 transgene under control of the Ins2 promoter was injected directly into NOD/Lt zygotes. In 2006, the T1DR received this transgenic strain at generation F?+33.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-GAD2)1Lt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Edward H Leiter | ||
| Common Name(s) | TgN(RIP7hGAD65)Lt4 Y-line; | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | GAD2, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), human | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | The transgene comprises the cDNA sequence encoding human glutamic acid decarboxylase 2 under control of a modified rat insulin 2 promoter composed of 9.5 kb of genomic DNA from upstream of the transcription initiation site fused to the first intron of Ins2. Transmission from the founder male only to male progeny demonstrated integration into the Y chromosome. Densitrometric Southern blot analysis indicates presence of approximately 17 copies of the transgene. Expression in the pancreatic islets of transgenic mRNA has been demonstrated by reverse transcription followed by PCR (RT-PCR), and of GAD2 protein by western blot analysis. [MGI Ref ID J:100251] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier female | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-GAD2)
Colony Maintenance
| Breeding & Husbandry | A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction. |
|---|
Related Strains
Strains carrying Tg(Ins2-GAD2)1Lt allele
003843 NOD.Cg-Prkdcscid Tg(Ins2-GAD2)1Lt/LtJ View Strains carrying Tg(Ins2-GAD2)1Lt (1 strain)
003844 NOD.Cg-Prkdcscid Tg(Ins2-GAD2)2Lt/LtJ 005870 NOD/ShiLt(Cg)-Tg(Ins2-GAD2)2Lt/J
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
References
Selected Reference(s)
Bridgett M; Cetkovic-Cvrlje M; O'Rourke R; Shi Y; Narayanswami S; Lambert J; Ramiya V; Baekkeskov S; Leiter EH. 1998. Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes 47(12):1848-56. [PubMed: 9836515] [MGI Ref ID J:100251]
Price and Supply Information
| Strain Name: | NOD/ShiLt-Tg(Ins2-GAD2)1Lt/LtJ |
| Stock Number: | 003074 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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