Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+2 (29-AUG-04)   Donating Investigator Michael Greenberg,   Childrens Hospital

Description
Homozygous mice are viable and fertile. Pups of female homozygotes need fostering.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Fosb

003762

B6.Cg-Tg(tetFosb)4468Nes/J

View Strains carrying other alleles of Fosb     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Fosb^tm1Meg/Fosb⁺

        involves: 129S4/SvJae * 129X1/SvJ * BALB/c

• behavior/neurological phenotype
• abnormal frequency of paradoxical sleep (MGI Ref ID J:64368)
  • mice show fewer numbers of REM sleep bouts than wild-type

Fosb^tm1Meg/Fosb^tm1Meg

        involves: 129S4/SvJae * 129X1/SvJ * BALB/c

• behavior/neurological phenotype
• abnormal frequency of paradoxical sleep (MGI Ref ID J:64368)
  • mutants display a significant decrease (41.5%) in REM sleep during lights-on periods; there is a significant decrease (31.5%) in REM sleep during the lights-out period compared to wild-type

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Fosb^tm1Meg/Fosb^tm1Meg

        involves: 129S4/SvJae * 129X1/SvJ

• behavior/neurological phenotype
• electrically induced seizures (MGI Ref ID J:110995)
  • 2-3 month old mutant mice show a significant delay in development of tolerance to motor seizures compared to wild-type mice; repeated ECS treatment does eventually result in a significant reduction in seizure duration, to wild-type levels by day 6 of treatment
• nervous system phenotype
• electrically induced seizures (MGI Ref ID J:110995)
  • 2-3 month old mutant mice show a significant delay in development of tolerance to motor seizures compared to wild-type mice; repeated ECS treatment does eventually result in a significant reduction in seizure duration, to wild-type levels by day 6 of treatment
• reduced NMDA -mediated synaptic currents (MGI Ref ID J:110995)
  • in layer II/III pyramidal cells, repeated ECS treatments does not alter 25 um NMDA-induced inward current but currents in wild-type cells are significantly enhanced in brain slices from 2-3 month old mutants

Fosb^tm1Meg/Fosb^tm1Meg

        either: (involves: 129S4/SvJae * 129X1/SvJ) or (involves: 129S4/SvJae * 129X1/SvJ * BALB/c)

• behavior/neurological phenotype
• abnormal parental behavior (MGI Ref ID J:34461)
  • young homozygous males display defective nurturing behavior illustrated by impaired pup retrieval compared to wild-type males
• abnormal maternal nurturing (MGI Ref ID J:34461)
  • majority of pups die within 1 to 2 days of birth regardless of their genotype
  • homozygous pups will survive if fostered by wild-type or heterozygous females
  • if homozygous females are mated to homozygous, heterozygous or wild-type males on a 129 background, 0-1 pups on average survive to weaning regardless of pup genotype; if homozygous males are mated to heterozygous or wild-type males, 3-5 pups survive to weaning regardless of pup genotype
  • if homozygous females are mated to homozygous, heterozygous or wild-type males on 129/B6 background, only 1-2 pups survive to weaning regardless of pup genotype; if homozygous males are mated to heterozygous or wild-type males, 7-9 pups survive to weaning regardless of genotype
• abnormal nursing (MGI Ref ID J:34461)
  • homozygous females do not nurse their pups
• abnormal pup retrieval (MGI Ref ID J:34461)
  • if 3 pups are removed from the cage then replaced shortly after, homozygous females take 50 times longer to retrieve the first pup compared to wild-type or heterozygotes and only 50% retrieve even one pup
• growth/size phenotype
• decreased body size (MGI Ref ID J:34461)
  • homozygotes are about ~10% smaller than wild-type

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Fosb^tm1Meg related

Neurobiology Research
Behavioral and Learning Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Fosbtm1Meg
Allele Name		targeted mutation 1, Michael E Greenburg
Allele Type		Targeted (knock-out)
Common Name(s)		B-; FosB-;
Mutation Made By		Michael Greenberg,   Childrens Hospital
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Fosb, FBJ osteosarcoma oncogene B
Chromosome		7
Gene Common Name(s)		AP-1; DKFZp686C0818; G0S3; GOS3; GOSB; MGC42291; fra-2;
Molecular Note		A neomycin selection cassette was inserted into exon 2, 5' to the sequences encoding the basic region and adjacent to the sequences encoding the transactivation domain. Northern blot analysis on RNA derived from serum-starved, stimulated embryonic fibroblasts derived from homozygous mice demonstrated that no detectable transcript is produced from this allele. Western blot analysis on these same cells confirmed that no detectable protein is encoded by this allele. [MGI Ref ID J:34461]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Brown JR; Ye H; Bronson RT; Dikkes P; Greenberg ME. 1996. A defect in nurturing in mice lacking the immediate early gene fosB. Cell 86(2):297-309. [PubMed: 8706134]  [MGI Ref ID J:34461]

Additional References

Fosb^tm1Meg related

Brown JR; Nigh E; Lee RJ; Ye H; Thompson MA; Saudou F; Pestell RG; Greenberg ME. 1998. Fos family members induce cell cycle entry by activating cyclin D1. Mol Cell Biol 18(9):5609-19. [PubMed: 9710644]  [MGI Ref ID J:111015]

Hiroi N; Brown JR; Haile CN; Ye H; Greenberg ME; Nestler EJ. 1997. FosB mutant mice: loss of chronic cocaine induction of Fos-related proteins and heightened sensitivity to cocaine's psychomotor and rewarding effects. Proc Natl Acad Sci U S A 94(19):10397-402. [PubMed: 9294222]  [MGI Ref ID J:42926]

Hiroi N; Marek GJ; Brown JR; Ye H; Saudou F; Vaidya VA; Duman RS; Greenberg ME; Nestler EJ. 1998. Essential role of the fosB gene in molecular, cellular, and behavioral actions of chronic electroconvulsive seizures. J Neurosci 18(17):6952-62. [PubMed: 9712664]  [MGI Ref ID J:110995]

Korkosz A; Kolomanska P; Kowalska K; Rogowski A; Radwanska K; Kaczmarek L; Mierzejewski P; Scinska A; Kostowski W; Bienkowski P. 2004. Dissociation of ethanol and saccharin preference in fosB knockout mice. Physiol Behav 82(2-3):391-5. [PubMed: 15276803]  [MGI Ref ID J:102266]

Kuroda KO; Meaney MJ; Uetani N; Fortin Y; Ponton A; Kato T. 2007. ERK-FosB signaling in dorsal MPOA neurons plays a major role in the initiation of parental behavior in mice. Mol Cell Neurosci 36(2):121-31. [PubMed: 17707653]  [MGI Ref ID J:126793]

Kuroda KO; Meaney MJ; Uetani N; Kato T. 2008. Neurobehavioral basis of the impaired nurturing in mice lacking the immediate early gene FosB. Brain Res 1211:57-71. [PubMed: 18423429]  [MGI Ref ID J:136816]

Malik MT; Peng YJ; Kline DD; Adhikary G; Prabhakar NR. 2005. Impaired ventilatory acclimatization to hypoxia in mice lacking the immediate early gene fos B. Respir Physiol Neurobiol 145(1):23-31. [PubMed: 15652785]  [MGI Ref ID J:101879]

Shiromani PJ; Basheer R; Thakkar J; Wagner D; Greco MA; Charness ME. 2000. Sleep and wakefulness in c-fos and fos B gene knockout mice Brain Res Mol Brain Res 80(1):75-87. [PubMed: 11039731]  [MGI Ref ID J:64368]

Zhu H; Lee M; Agatsuma S; Hiroi N. 2007. Pleiotropic impact of constitutive fosB inactivation on nicotine-induced behavioral alterations and stress-related traits in mice. Hum Mol Genet 16(7):820-36. [PubMed: 17468183]  [MGI Ref ID J:121766]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	None Available
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.