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Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6 Donor Strain 129x1 x 129S1 via R1 (+Kitl-SlJ) ES cell line Generation N5F8N1p Donating Investigator Michael Meyer, Ludwig-Maximilians-University Munich Appearance
black, ataxic
Related Genotype: a/a Calb1tm1Mpin/Calb 1tm1MpinDescription
Mice homozygous for the Calb1tm1Mpin targeted mutation are viable and fertile. Homozygous mutant mice show severe impairment in tests of motor coordination and exhibit ataxia when challenged on a runway. These results suggest functional deficits in cerebellar pathways. Confocal calcium imaging of cerebellar Purkinje cells reveals marked changes of synaptically-evoked postsynaptic calcium transients.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Congenic Nomenclature
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Calb1tm1Mpin/Calb1+
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- behavior/neurological phenotype
- ataxia (MGI Ref ID J:38599)
- 2-month old heterozygotes show significant deficits in initial trials in a thin horizontal rod test compared to controls but are significantly better than homozygotes; after first 2 sessions, no significant difference remains between mutant groups
- nervous system phenotype
- abnormal nervous system electrophysiology (MGI Ref ID J:38599)
- heterozygotes show a significant increase in dendritic calcium transients upon climbing fiber stimulation compared to controls
Calb1tm1Mpin/Calb1tm1Mpin
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- behavior/neurological phenotype
- ataxia (MGI Ref ID J:38599)
- mutants do not display ataxia in standard environments; when challenged (with a runway test or rotarod test) coordination is severely disturbed compared to controls
- mice display a shaky tremor which usually worsens during movement; controls do not show any tremors
- nervous system phenotype
- abnormal nervous system electrophysiology (MGI Ref ID J:38599)
- dendritic calcium transients associated with complex spikes (synaptic response) upon climbing fiber stimulation is altered in mutants compared to controls; peak amplitudes are enhanced by ~80% over wild-type
- hearing/vestibular/ear phenotype
- *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:60520)
- at 4-8 months, homozygotes exhibit normal cochlear morphology, as well as normal hearing and cochlear OHC function, as assessed by baseline auditory brainstem evoked responses and DPOAEs, respectively
- at 3 weeks after exposure to a moderate noise trauma, homozygotes exhibit a similar loss of cochlear hair cells to wild-type mice, with no significant differences in permanent threshold shifts of ABR or DPOAE amplitudes
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Calb1tm1Mpin related
Apoptosis Research
Endogenous Regulators
Metabolism Research
Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Metabolic Defects
Neurodegeneration
| Allele Symbol | Calb1tm1Mpin | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Max-Planck-Institute for Neurobiology | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | CaBP-; Cb-; calbindin D28K -; | ||
| Mutation Made By | Michael Meyer, Ludwig-Maximilians-University Munich | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Calb1, calbindin 1 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | Brain-2; CALB; CB; CaBP28K; Calb; Calb-1; MGC93326; calbindin-D28K; | ||
| Molecular Note | A genomic fragment containing the promoter and first exon was replaced with a neomycin selection cassette. Western blot and immunohistochemistry analysis on tissues derived from homozygous mice demonstrated that no detectable protein was produced from this allele. [MGI Ref ID J:38599] | ||
Genotyping Protocols
Calb1tm1Mpin, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Airaksinen MS; Thoenen H; Meyer M. 1997. Vulnerability of midbrain dopaminergic neurons in calbindin-D28k-deficient mice: lack of evidence for a neuroprotective role of endogenous calbindin in MPTP-treated and weaver mice. Eur J Neurosci 9(1):120-7. [PubMed: 9042576] [MGI Ref ID J:40696]
Fletcher C; Norman DJ; Heintz N. 1991. Genetic mapping of meander tail, a mouse mutation affecting cerebellar development. Genomics 9(4):647-55. [PubMed: 2037292] [MGI Ref ID J:11192]
Frantz GD; Tobin AJ. 1994. Cellular distribution of calbindin D28K mRNAs in the adult mouse brain. J Neurosci Res 37(3):287-302. [PubMed: 8176753] [MGI Ref ID J:16904]
Schiffmann SN; Cheron G; Lohof A; d'Alcantara P; Meyer M; Parmentier M; Schurmans S. 1999. Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. Proc Natl Acad Sci U S A 96(9):5257-62. [PubMed: 10220453] [MGI Ref ID J:54517]
Sooy K; Schermerhorn T; Noda M; Surana M; Rhoten WB; Meyer M; Fleischer N; Sharp GW; Christakos S. 1999. Calbindin-D(28k) controls [Ca(2+)](i) and insulin release. Evidence obtained from calbindin-d(28k) knockout mice and beta cell lines. J Biol Chem 274(48):34343-9. [PubMed: 10567411] [MGI Ref ID J:58854]
Calb1tm1Mpin relatedAiraksinen L; Virkkala J; Aarnisalo A; Meyer M; Ylikoski J; Airaksinen MS. 2000. Lack of calbindin-D28k does not affect hearing level or survival of hair cells in acoustic trauma. ORL J Otorhinolaryngol Relat Spec 62(1):9-12. [PubMed: 10654311] [MGI Ref ID J:60520]
Airaksinen MS; Eilers J; Garaschuk O; Thoenen H; Konnerth A; Meyer M. 1997. Ataxia and altered dendritic calcium signaling in mice carrying a targeted null mutation of the calbindin D28k gene. Proc Natl Acad Sci U S A 94(4):1488-93. [PubMed: 9037080] [MGI Ref ID J:38599]
Airaksinen MS; Thoenen H; Meyer M. 1997. Vulnerability of midbrain dopaminergic neurons in calbindin-D28k-deficient mice: lack of evidence for a neuroprotective role of endogenous calbindin in MPTP-treated and weaver mice. Eur J Neurosci 9(1):120-7. [PubMed: 9042576] [MGI Ref ID J:40696]
Bouilleret V; Schwaller B; Schurmans S; Celio MR; Fritschy JM. 2000. Neurodegenerative and morphogenic changes in a mouse model of temporal lobe epilepsy do not depend on the expression of the calcium-binding proteins parvalbumin, calbindin, or calretinin Neuroscience 97(1):47-58. [PubMed: 10771338] [MGI Ref ID J:62357]
Bouslama-Oueghlani L; Wehrle R; Sotelo C; Dusart I. 2003. The developmental loss of the ability of Purkinje cells to regenerate their axons occurs in the absence of myelin: an in vitro model to prevent myelination. J Neurosci 23(23):8318-29. [PubMed: 12967994] [MGI Ref ID J:85604]
Chen G; Racay P; Bichet S; Celio MR; Eggli P; Schwaller B. 2006. Deficiency in parvalbumin, but not in calbindin D-28k upregulates mitochondrial volume and decreases smooth endoplasmic reticulum surface selectively in a peripheral, subplasmalemmal region in the soma of Purkinje cells. Neuroscience 142(1):97-105. [PubMed: 16860487] [MGI Ref ID J:113147]
Cheron G; Gall D; Servais L; Dan B; Maex R; Schiffmann SN. 2004. Inactivation of calcium-binding protein genes induces 160 Hz oscillations in the cerebellar cortex of alert mice. J Neurosci 24(2):434-41. [PubMed: 14724241] [MGI Ref ID J:87450]
Gkika D; Hsu YJ; van der Kemp AW; Christakos S; Bindels RJ; Hoenderop JG. 2006. Critical role of the epithelial Ca2+ channel TRPV5 in active Ca2+ reabsorption as revealed by TRPV5/calbindin-D28K knockout mice. J Am Soc Nephrol 17(11):3020-7. [PubMed: 17005931] [MGI Ref ID J:135840]
Kriegsfeld LJ; Mei DF; Yan L; Witkovsky P; Lesauter J; Hamada T; Silver R. 2008. Targeted mutation of the calbindin D28K gene disrupts circadian rhythmicity and entrainment. Eur J Neurosci 27(11):2907-21. [PubMed: 18588531] [MGI Ref ID J:137158]
Lalonde R; Strazielle C. 2007. Spontaneous and induced mouse mutations with cerebellar dysfunctions: behavior and neurochemistry. Brain Res 1140:51-74. [PubMed: 16499884] [MGI Ref ID J:120621]
Muller A; Kukley M; Uebachs M; Beck H; Dietrich D. 2007. Nanodomains of single Ca2+ channels contribute to action potential repolarization in cortical neurons. J Neurosci 27(3):483-95. [PubMed: 17234581] [MGI Ref ID J:117436]
Servais L; Bearzatto B; Schwaller B; Dumont M; De Saedeleer C; Dan B; Barski JJ; Schiffmann SN; Cheron G. 2005. Mono- and dual-frequency fast cerebellar oscillation in mice lacking parvalbumin and/or calbindin D-28k. Eur J Neurosci 22(4):861-70. [PubMed: 16115209] [MGI Ref ID J:100990]
Servais L; Cheron G. 2005. Purkinje cell rhythmicity and synchronicity during modulation of fast cerebellar oscillation. Neuroscience 134(4):1247-59. [PubMed: 16054763] [MGI Ref ID J:104422]
Sooy K; Schermerhorn T; Noda M; Surana M; Rhoten WB; Meyer M; Fleischer N; Sharp GW; Christakos S. 1999. Calbindin-D(28k) controls [Ca(2+)](i) and insulin release. Evidence obtained from calbindin-d(28k) knockout mice and beta cell lines. J Biol Chem 274(48):34343-9. [PubMed: 10567411] [MGI Ref ID J:58854]
Turnbull CI; Looi K; Mangum JE; Meyer M; Sayer RJ; Hubbard MJ. 2004. Calbindin independence of calcium transport in developing teeth contradicts the calcium ferry dogma. J Biol Chem 279(53):55850-4. [PubMed: 15494408] [MGI Ref ID J:95037]
Vecellio M; Schwaller B; Meyer M; Hunziker W; Celio MR. 2000. Alterations in Purkinje cell spines of calbindin D-28 k and parvalbumin knock-out mice. Eur J Neurosci 12(3):945-54. [PubMed: 10762324] [MGI Ref ID J:89904]
Westerink RH; Rook MB; Beekwilder JP; Wadman WJ. 2006. Dual role of calbindin-D28K in vesicular catecholamine release from mouse chromaffin cells. J Neurochem 99(2):628-40. [PubMed: 16824046] [MGI Ref ID J:119287]
Zheng W; Xie Y; Li G; Kong J; Feng JQ; Li YC. 2004. Critical role of calbindin-D28k in calcium homeostasis revealed by mice lacking both vitamin D receptor and calbindin-D28k. J Biol Chem 279(50):52406-13. [PubMed: 15456794] [MGI Ref ID J:95170]
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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