Description

Strain Information

Former Names B6;129P-Emx1tm1Jlr/J    (Changed: 18-OCT-05 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+7p   Donating Investigator John Rubenstein,   Univ of Calif, San Francisco

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Emx1 ^tm1Jlr targeted mutation are viable and fertile. They show no obvious morphological or behavioral abnormalities. However, all homozygous mice lack most or all of their corpus callosum, the principle fiber tract that connects the left and right cerebral hemispheres. Heterozygotes show partial penetrance for the corpus callosum abnormality. The cerebral cortex appears normal in both heterozygous and homozygous mice.

Development
A targeting vector containing PGKneo gene cassette was used to disrupt exon 1 and part of intron 1, encoding the N-terminus. The construct was electroporated into 129X1/SvJ derived JM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to generate homozygotes.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Emx1

005628

B6.129S2-Emx1tm1(cre)Krj/J

View Strains carrying other alleles of Emx1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Emx1^tm1Jlr/Emx1⁺

        involves: 129X1/SvJ * C57BL/6

• nervous system phenotype
• abnormal corpus callosum morphology (MGI Ref ID J:35060)
  • incomplete penetrance; observed in 5 of 15 heterozygous mice

Emx1^tm1Jlr/Emx1^tm1Jlr

        involves: 129X1/SvJ * C57BL/6

• nervous system phenotype
• absent corpus callosum (MGI Ref ID J:35060)
  • all homozygous mutant mice lack most or all of the corpus collosum; histological analysis revealed a small interhemispheric commissure, which may be residual corpus collosum

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Emx1^tm1Jlr related

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Emx1tm1Jlr
Allele Name		targeted mutation 1, John Rubenstein
Allele Type		Targeted (knock-out)
Common Name(s)		Emx-1-; Emx1null;
Mutation Made By		John Rubenstein,   Univ of Calif, San Francisco
Strain of Origin		129X1/SvJ
ES Cell Line Name		JM-1
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Emx1, empty spiracles homolog 1 (Drosophila)
Chromosome		6
Gene Common Name(s)		RGD1564002;
Molecular Note		Insertion of a PGK-neomycin resistance cassette replaced exon 1 and part of intron 1 of the Emx1 gene. Northern analysis of E14.5 embryos did not detect Emx1 transcript in homozygous mutant mice. The authors predict that the mutant allele would produce atruncated protein that lacks the N-terminus. [MGI Ref ID J:35060]

Genotyping

Genotyping Information

Genotyping Protocols

Emx1^tm1Jlr, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Qiu M; Anderson S; Chen S; Meneses JJ; Hevner R; Kuwana E; Pedersen RA; Rubenstein JL. 1996. Mutation of the Emx-1 homeobox gene disrupts the corpus callosum. Dev Biol 178(1):174-8. [PubMed: 8812118]  [MGI Ref ID J:35060]

Additional References

Emx1^tm1Jlr related

Bishop KM; Garel S; Nakagawa Y; Rubenstein JL; O'Leary DD. 2003. Emx1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J Comp Neurol 457(4):345-60. [PubMed: 12561075]  [MGI Ref ID J:82130]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain is maintained by homozyous sibling matings. Expected coat color from breeding:Black
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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