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Description
Strain Information
Former Names 129S3/-etc (Changed: 15-DEC-04 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N1 Donating Investigator Michael Meyer, Ludwig-Maximilians-University Munich Description
Heterozygous mice exhibit expression of beta-galactosidase in populations of large sensory neurons. Mice homozygous for the Bcl2tm1Mpin targeted mutation do not produce either the a or b form of the Bcl2 protein. Bcl2 is a major regulator of programmed cell death, a critical process in shaping the developing nervous system. The absence of the Bcl2 does not significantly influence the development of motor neurons before or during the main period of physiological cell death. Rather, Bcl2 exerts its influence beyond this period, subsequent to the phase where the majority of neuronal loss normally takes place. Polycystic kidney disease is less severe in this strain compared to the Bcl2tm1Sjk targeted mutation (Stock No. 002265).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (178 strains)
002265 B6;129S2-Bcl2tm1Sjk/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Bcl2tm1Mpin/Bcl2tm1Mpin
involves: 129S2/SvPas * C57BL/6
- lethality-postnatal
- postnatal lethality (MGI Ref ID J:34326)
- onset of mortality ranges from 2 to 24 weeks
- life span-post-weaning/aging
- premature death (MGI Ref ID J:34326)
- onset of mortality ranges from 2 to 24 weeks, with a clustering at 7-8 weeks; some live substantially longer
- growth/size phenotype
- decreased body size (MGI Ref ID J:34326)
- weight becomes progressively smaller than wild-type, with no differences seen at P9 but not at P3
- renal/urinary system phenotype
- polycystic kidney (MGI Ref ID J:34326)
- nervous system phenotype
- abnormal L3 dorsal root ganglion morphology (MGI Ref ID J:34326)
- exhibit a progressive decrease in the number of neurons present in the third lumbar dorsal root ganglion, amounting to 90% of wild-type at P9 and 56% at P44
- subpopulation of large sensory neurons is more severely affected than the small sensory neurons
- abnormal facial motor nucleus morphology (MGI Ref ID J:34326)
- degeneration of motoneurons occurs predominately in the lateral part of the facial nucleus
- abnormal optic nerve morphology (MGI Ref ID J:54876)
- show decreased cross-sectional area of optic nerve, reflecting a 29% loss of retinal ganglion cell axons, at P15 but not at P10, the time after the period of naturally occurring cell death
- abnormal superior cervical ganglion morphology (MGI Ref ID J:34326)
- exhibit a progressive decrease in the number of superior cervical ganglia neurons, amounting to 60% of wild-type at P10 and 58% at P44
- abnormal sympathetic neuron morphology (MGI Ref ID J:34326)
- progressive degeneration of sympathetic neurons during early postnatal development
- decreased sensory neuron number (MGI Ref ID J:34326)
- progressive degeneration of sensory neurons during early postnatal development
- motor neuron degeneration (MGI Ref ID J:34326)
- progressive degeneration of facial motoneurons during early postnatal development, before P9
- at P9, P28, and P44, the number of facial motoneurons is only 71%, 68%, and 67%, respectively, of the number present in wild-type
- although exhibit motoneuron degeneration, motoneurons are not more vulnerable than wild-type to injury at P28
- retinal ganglion cell degeneration (MGI Ref ID J:54876)
- retinal ganglion cell loss after the period of naturally occurring cell death
- homeostasis/metabolism phenotype
- increased blood urea nitrogen level (MGI Ref ID J:34326)
- exhibit a 2-fold increase at 10 weeks of age, but not at P13
- increased circulating creatinine level (MGI Ref ID J:34326)
- exhibit a 1.7-fold increase at 10 weeks of age, but not at P13
- vision/eye phenotype
- abnormal optic nerve morphology (MGI Ref ID J:54876)
- show decreased cross-sectional area of optic nerve, reflecting a 29% loss of retinal ganglion cell axons, at P15 but not at P10, the time after the period of naturally occurring cell death
- retinal ganglion cell degeneration (MGI Ref ID J:54876)
- retinal ganglion cell loss after the period of naturally occurring cell death
This mouse can be used to support research in many areas including:
Bcl2tm1Mpin relatedDevelopmental Biology Research
Neurodevelopmental Defects
Internal/Organ Research
Kidney Defects (polycystic kidney disease)
Neurobiology Research
lacZ expression in neural tissue
Neurodevelopmental Defects
Research Tools
lacZ Expression
Apoptosis Research
Neurobiology Research (cell marker)
Apoptosis Research
Endogenous Regulators
Cancer Research
Tumor Suppressor Genes
Developmental Biology Research
Growth Defects
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Immunodeficiency
Intracellular Signaling Molecules
Internal/Organ Research
Kidney Defects (polycystic kidney disease)
Spleen Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Bcl2tm1Mpin | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Max-Planck-Institute for Neurobiology | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | bcl-2 -; | ||
| Mutation Made By | Michael Meyer, Ludwig-Maximilians-University Munich | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Site of Expression | large sensory neurons; Bcl2 (B-cell leukemia/lymphoma 2) gene products not produced | ||
| Gene Symbol and Name | Bcl2, B-cell leukemia/lymphoma 2 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | AW986256; Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik; RIKEN cDNA C430015F12 gene; RIKEN cDNA D630044D05 gene; RIKEN cDNA D830018M01 gene; expressed sequence AW986256; | ||
| Molecular Note | Exon 2 was disrupted by the insertion of a lacZ gene and neomycin selection cassette. Western blot analysis on hippocampal lysates from homozygous mice demonstrated that no stable Bcl2 protein was made from this allele. A beta-galactosidase protein wasproduced from this allele under the control of the endogenous promoter. [MGI Ref ID J:34326] [MGI Ref ID J:86581] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Bcl2tm1Mpin, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Michaelidis TM; Sendtner M; Cooper JD; Airaksinen MS; Holtmann B; Meyer M; Thoenen H. 1996. Inactivation of bcl-2 results in progressive degeneration of motoneurons, sympathetic and sensory neurons during early postnatal development. Neuron 17(1):75-89. [PubMed: 8755480] [MGI Ref ID J:34326]
Additional References
Boot-Handford RP; Michaelidis TM; Hillarby MC; Zambelli A; Denton J; Hoyland JA; Freemont AJ; Grant ME; Wallis GA. 1998. The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype. Int J Exp Pathol 79(5):329-35. [PubMed: 10193316] [MGI Ref ID J:50619]
Cellerino A; Michaelidis T; Barski JJ; Bahr M; Thoenen H; Meyer M. 1999. Retinal ganglion cell loss after the period of naturally occurring cell death in bcl-2-/- mice. Neuroreport 10(5):1091-5. [PubMed: 10321489] [MGI Ref ID J:54876]
Gillardon F; Moll I; Meyer M; Michaelidis TM. 1999. Alterations in cell death and cell cycle progression in the UV-irradiated epidermis of bcl-2-deficient mice. Cell Death Differ 6(1):55-60. [PubMed: 10200548] [MGI Ref ID J:52893]
Hata R; Gillardon F; Michaelidis TM; Hossmann KA. 1999. Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury. Metab Brain Dis 14(2):117-24. [PubMed: 10488913] [MGI Ref ID J:59492]
Bcl2tm1Mpin relatedBoot-Handford RP; Michaelidis TM; Hillarby MC; Zambelli A; Denton J; Hoyland JA; Freemont AJ; Grant ME; Wallis GA. 1998. The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype. Int J Exp Pathol 79(5):329-35. [PubMed: 10193316] [MGI Ref ID J:50619]
Brunner C; Marinkovic D; Klein J; Samardzic T; Nitschke L; Wirth T. 2003. B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice. J Exp Med 197(9):1205-11. [PubMed: 12732662] [MGI Ref ID J:83281]
Cellerino A; Michaelidis T; Barski JJ; Bahr M; Thoenen H; Meyer M. 1999. Retinal ganglion cell loss after the period of naturally occurring cell death in bcl-2-/- mice. Neuroreport 10(5):1091-5. [PubMed: 10321489] [MGI Ref ID J:54876]
Einat H; Yuan P; Manji HK. 2005. Increased anxiety-like behaviors and mitochondrial dysfunction in mice with targeted mutation of the Bcl-2 gene: further support for the involvement of mitochondrial function in anxiety disorders. Behav Brain Res 165(2):172-80. [PubMed: 16095731] [MGI Ref ID J:115732]
Fedorov LM; Schmittwolf C; Amann K; Thomas WH; Muller AM; Schubert H; Domen J; Kneitz B. 2006. Renal failure causes early death of bcl-2 deficient mice. Mech Ageing Dev 127(7):600-9. [PubMed: 16620920] [MGI Ref ID J:110243]
Fedorov LM; Tyrsin OY; Papadopoulos T; Camarero G; Gotz R; Rapp UR. 2002. Bcl-2 Determines Susceptibility to Induction of Lung Cancer by Oncogenic CRaf. Cancer Res 62(21):6297-303. [PubMed: 12414660] [MGI Ref ID J:79696]
Gillardon F; Moll I; Meyer M; Michaelidis TM. 1999. Alterations in cell death and cell cycle progression in the UV-irradiated epidermis of bcl-2-deficient mice. Cell Death Differ 6(1):55-60. [PubMed: 10200548] [MGI Ref ID J:52893]
Hata R; Gillardon F; Michaelidis TM; Hossmann KA. 1999. Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury. Metab Brain Dis 14(2):117-24. [PubMed: 10488913] [MGI Ref ID J:59492]
Tzimagiorgis G; Michaelidis TM; Lindholm D; Thoenen H. 1996. Introduction of the negative selection marker into replacement vectors by a single ligation step. Nucleic Acids Res 24(17):3476-7. [PubMed: 8811108] [MGI Ref ID J:86581]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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|
|
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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