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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129S3/-etc (Changed: 15-DEC-04 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Michael Meyer, Ludwig-Maximilians-University Munich Description
Heterozygous mice exhibit expression of beta-galactosidase in populations of large sensory neurons. Mice homozygous for the Bcl2tm1Mpin targeted mutation do not produce either the a or b form of the Bcl2 protein. Bcl2 is a major regulator of programmed cell death, a critical process in shaping the developing nervous system. The absence of the Bcl2 does not significantly influence the development of motor neurons before or during the main period of physiological cell death. Rather, Bcl2 exerts its influence beyond this period, subsequent to the phase where the majority of neuronal loss normally takes place. Polycystic kidney disease is less severe in this strain compared to the Bcl2tm1Sjk targeted mutation (Stock No. 002265).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
018430 B6.129X1(Cg)-Bcl2tm1.1Sjk/J 003069 B6.NOD-(D1Mit3-Bcl2) (D17Mit21-D17Mit10)/LtJ 003062 B6.NOD-(D1Mit3-Bcl2)/J 002265 B6;129S2-Bcl2tm1Sjk/J 008882 STOCK Bcl2tm1Irt/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. B-Cell Cll/Lymphoma 2; BCL2 (BCL2)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Bcl2tm1Mpin/Bcl2tm1Mpin
involves: 129S2/SvPas * C57BL/6
- mortality/aging
- premature death
- onset of mortality ranges from 2 to 24 weeks, with a clustering at 7-8 weeks; some live substantially longer (MGI Ref ID J:34326)
- growth/size phenotype
- decreased body size
- weight becomes progressively smaller than wild-type, with no differences seen at P9 but not at P3 (MGI Ref ID J:34326)
- renal/urinary system phenotype
- polycystic kidney (MGI Ref ID J:34326)
- nervous system phenotype
- abnormal L3 dorsal root ganglion morphology
- abnormal facial motor nucleus morphology
- degeneration of motoneurons occurs predominately in the lateral part of the facial nucleus (MGI Ref ID J:34326)
- abnormal optic nerve morphology
- show decreased cross-sectional area of optic nerve, reflecting a 29% loss of retinal ganglion cell axons, at P15 but not at P10, the time after the period of naturally occurring cell death (MGI Ref ID J:54876)
- abnormal superior cervical ganglion morphology
- exhibit a progressive decrease in the number of superior cervical ganglia neurons, amounting to 60% of wild-type at P10 and 58% at P44 (MGI Ref ID J:34326)
- abnormal sympathetic neuron morphology
- progressive degeneration of sympathetic neurons during early postnatal development (MGI Ref ID J:34326)
- decreased sensory neuron number
- progressive degeneration of sensory neurons during early postnatal development (MGI Ref ID J:34326)
- motor neuron degeneration
- progressive degeneration of facial motoneurons during early postnatal development, before P9 (MGI Ref ID J:34326)
- at P9, P28, and P44, the number of facial motoneurons is only 71%, 68%, and 67%, respectively, of the number present in wild-type (MGI Ref ID J:34326)
- although exhibit motoneuron degeneration, motoneurons are not more vulnerable than wild-type to injury at P28 (MGI Ref ID J:34326)
- retinal ganglion cell degeneration
- retinal ganglion cell loss after the period of naturally occurring cell death (MGI Ref ID J:54876)
- homeostasis/metabolism phenotype
- increased blood urea nitrogen level
- exhibit a 2-fold increase at 10 weeks of age, but not at P13 (MGI Ref ID J:34326)
- increased circulating creatinine level
- exhibit a 1.7-fold increase at 10 weeks of age, but not at P13 (MGI Ref ID J:34326)
- vision/eye phenotype
- abnormal optic nerve morphology
- show decreased cross-sectional area of optic nerve, reflecting a 29% loss of retinal ganglion cell axons, at P15 but not at P10, the time after the period of naturally occurring cell death (MGI Ref ID J:54876)
- retinal ganglion cell degeneration
- retinal ganglion cell loss after the period of naturally occurring cell death (MGI Ref ID J:54876)
This mouse can be used to support research in many areas including:
Bcl2tm1Mpin relatedDevelopmental Biology Research
Neurodevelopmental Defects
Internal/Organ Research
Kidney Defects
polycystic kidney disease
Neurobiology Research
lacZ expression in neural tissue
Neurodevelopmental Defects
Research Tools
lacZ Expression
Apoptosis Research
Neurobiology Research
cell marker
Apoptosis Research
Endogenous Regulators
Cancer Research
Tumor Suppressor Genes
Developmental Biology Research
Growth Defects
Hematological Research
Hematopoietic Defects
Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
Intracellular Signaling Molecules
Internal/Organ Research
Kidney Defects
polycystic kidney disease
Spleen Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Bcl2tm1Mpin | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Max-Planck-Institute for Neurobiology | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | bcl-2 -; | ||
| Mutation Made By | Dr. Michael Meyer, Ludwig-Maximilians-University Munich | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Site of Expression | large sensory neurons; Bcl2 (B-cell leukemia/lymphoma 2) gene products not produced | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | Exon 2 was disrupted by the insertion of a lacZ gene and neomycin selection cassette. Western blot analysis on hippocampal lysates from homozygous mice demonstrated that no stable Bcl2 protein was made from this allele. A beta-galactosidase protein wasproduced from this allele under the control of the endogenous promoter. [MGI Ref ID J:34326] [MGI Ref ID J:86581] | ||
| Gene Symbol and Name | Bcl2, B cell leukemia/lymphoma 2 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | AW986256; Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50; RIKEN cDNA C430015F12 gene; RIKEN cDNA D630044D05 gene; RIKEN cDNA D830018M01 gene; expressed sequence AW986256; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Bcl2tm1Mpin, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Michaelidis TM; Sendtner M; Cooper JD; Airaksinen MS; Holtmann B; Meyer M; Thoenen H. 1996. Inactivation of bcl-2 results in progressive degeneration of motoneurons, sympathetic and sensory neurons during early postnatal development. Neuron 17(1):75-89. [PubMed: 8755480] [MGI Ref ID J:34326]
Additional References
Boot-Handford RP; Michaelidis TM; Hillarby MC; Zambelli A; Denton J; Hoyland JA; Freemont AJ; Grant ME; Wallis GA. 1998. The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype. Int J Exp Pathol 79(5):329-35. [PubMed: 10193316] [MGI Ref ID J:50619]
Cellerino A; Michaelidis T; Barski JJ; Bahr M; Thoenen H; Meyer M. 1999. Retinal ganglion cell loss after the period of naturally occurring cell death in bcl-2-/- mice. Neuroreport 10(5):1091-5. [PubMed: 10321489] [MGI Ref ID J:54876]
Gillardon F; Moll I; Meyer M; Michaelidis TM. 1999. Alterations in cell death and cell cycle progression in the UV-irradiated epidermis of bcl-2-deficient mice. Cell Death Differ 6(1):55-60. [PubMed: 10200548] [MGI Ref ID J:52893]
Hata R; Gillardon F; Michaelidis TM; Hossmann KA. 1999. Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury. Metab Brain Dis 14(2):117-24. [PubMed: 10488913] [MGI Ref ID J:59492]
Bcl2tm1Mpin relatedBoot-Handford RP; Michaelidis TM; Hillarby MC; Zambelli A; Denton J; Hoyland JA; Freemont AJ; Grant ME; Wallis GA. 1998. The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype. Int J Exp Pathol 79(5):329-35. [PubMed: 10193316] [MGI Ref ID J:50619]
Brunner C; Marinkovic D; Klein J; Samardzic T; Nitschke L; Wirth T. 2003. B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice. J Exp Med 197(9):1205-11. [PubMed: 12732662] [MGI Ref ID J:83281]
Cellerino A; Michaelidis T; Barski JJ; Bahr M; Thoenen H; Meyer M. 1999. Retinal ganglion cell loss after the period of naturally occurring cell death in bcl-2-/- mice. Neuroreport 10(5):1091-5. [PubMed: 10321489] [MGI Ref ID J:54876]
Einat H; Yuan P; Manji HK. 2005. Increased anxiety-like behaviors and mitochondrial dysfunction in mice with targeted mutation of the Bcl-2 gene: further support for the involvement of mitochondrial function in anxiety disorders. Behav Brain Res 165(2):172-80. [PubMed: 16095731] [MGI Ref ID J:115732]
Fedorov LM; Schmittwolf C; Amann K; Thomas WH; Muller AM; Schubert H; Domen J; Kneitz B. 2006. Renal failure causes early death of bcl-2 deficient mice. Mech Ageing Dev 127(7):600-9. [PubMed: 16620920] [MGI Ref ID J:110243]
Fedorov LM; Tyrsin OY; Papadopoulos T; Camarero G; Gotz R; Rapp UR. 2002. Bcl-2 Determines Susceptibility to Induction of Lung Cancer by Oncogenic CRaf. Cancer Res 62(21):6297-303. [PubMed: 12414660] [MGI Ref ID J:79696]
Gillardon F; Moll I; Meyer M; Michaelidis TM. 1999. Alterations in cell death and cell cycle progression in the UV-irradiated epidermis of bcl-2-deficient mice. Cell Death Differ 6(1):55-60. [PubMed: 10200548] [MGI Ref ID J:52893]
Hata R; Gillardon F; Michaelidis TM; Hossmann KA. 1999. Targeted disruption of the bcl-2 gene in mice exacerbates focal ischemic brain injury. Metab Brain Dis 14(2):117-24. [PubMed: 10488913] [MGI Ref ID J:59492]
Tzimagiorgis G; Michaelidis TM; Lindholm D; Thoenen H. 1996. Introduction of the negative selection marker into replacement vectors by a single ligation step. Nucleic Acids Res 24(17):3476-7. [PubMed: 8811108] [MGI Ref ID J:86581]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
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