Description

Strain Information

Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N8F15p   Donating Investigator David Serreze,   The Jackson Laboratory

Control Information

	Control
	Multiple; Inquire
Considerations for Choosing Controls

Related Strains

Strains carrying   Cd4^tm1Knw allele

002269	B6.129S6-Cd4tm1Knw/J
002268	B6;129S6-Cd4tm1Knw/J

View Strains carrying   Cd4^tm1Knw     (2 strains)

Strains carrying other alleles of Cd4

002447	B10.129S2(B6)-Cd4tm1Litt/J
002663	B6.129S2-Cd4tm1Mak/J
002664	B6;129S-Cd4tm1Mak Cd8atm1Mak/J
006483	CBy.129S2(B6)-Cd4tm1Mak/J
005328	NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J

View Strains carrying other alleles of Cd4     (5 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

NOT	Diabetes Mellitus, Insulin-Dependent; IDDM - No similarity to the expected human disease phenotype was found.4

4 One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of those genes, but the phenotype did not resemble the disease.

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cd4^tm1Knw/Cd4^tm1Knw

        NOD.Cg-Cd4^tm1Knw

• immune system phenotype
• decreased susceptibility to autoimmune diabetes (MGI Ref ID J:93553)
  • Cd4-deficient mice are diabetes resistant (diabetes observed in 0/18 females by 30 weeks)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)

Cd4^tm1Knw related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd4tm1Knw
Allele Name		targeted mutation 1, Barbara B Knowles
Allele Type		Targeted (knock-out)
Common Name(s)		CD4-; CD4D;
Mutation Made By		Barbara Knowles,   The Jackson Laboratory
Strain of Origin		129S/SvEv-Gpi1
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Cd4, CD4 antigen
Chromosome		6
Gene Common Name(s)		CD4mut; L3T4; Ly-4; W3/25; lymphocyte antigen 4; p55;
Molecular Note		A neomycin resistance gene was inserted into exon 5. [MGI Ref ID J:48775]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cd4, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754]  [MGI Ref ID J:93553]

Cd4^tm1Knw related

Bommireddy R; Engle SJ; Ormsby I; Boivin GP; Babcock GF; Doetschman T. 2004. Elimination of both CD4(+) and CD8(+) T cells but not B cells eliminates inflammation and prolongs the survival of TGFbeta1-deficient mice. Cell Immunol 232(1-2):96-104. [PubMed: 15922720]  [MGI Ref ID J:99033]

Borchers MT; Crosby J; Justice P; Farmer S; Hines E; Lee JJ; Lee NA. 2001. Intrinsic AHR in IL-5 transgenic mice is dependent on CD4(+) cells and CD49d-mediated signaling. Am J Physiol Lung Cell Mol Physiol 281(3):L653-9. [PubMed: 11504693]  [MGI Ref ID J:132284]

Crosby JR; Shen HH; Borchers MT; Justice JP; Ansay T; Lee JJ; Lee NA. 2002. Ectopic expression of IL-5 identifies an additional CD4(+) T cell mechanism of airway eosinophil recruitment. Am J Physiol Lung Cell Mol Physiol 282(1):L99-108. [PubMed: 11741821]  [MGI Ref ID J:107840]

Ganta RR; Cheng C; Wilkerson MJ; Chapes SK. 2004. Delayed clearance of Ehrlichia chaffeensis infection in CD4+ T-cell knockout mice. Infect Immun 72(1):159-67. [PubMed: 14688093]  [MGI Ref ID J:87828]

Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754]  [MGI Ref ID J:93553]

Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890]  [MGI Ref ID J:125272]

Hancock WW; Tsai TL; Madaio MP; Gasser DL. 2003. Cutting Edge: Multiple autoimmune pathways in kd/kd mice. J Immunol 171(6):2778-81. [PubMed: 12960297]  [MGI Ref ID J:85380]

Justice JP; Crosby J; Borchers MT; Tomkinson A; Lee JJ; Lee NA. 2002. CD4(+) T cell-dependent airway mucus production occurs in response to IL-5 expression in lung. Am J Physiol Lung Cell Mol Physiol 282(5):L1066-74. [PubMed: 11943672]  [MGI Ref ID J:108354]

Kassiotis G; Bauer J; Akassoglou K; Lassmann H; Kollias G; Probert L. 1999. A tumor necrosis factor-induced model of human primary demyelinating diseases develops in immunodeficient mice. Eur J Immunol 29(3):912-7. [PubMed: 10092095]  [MGI Ref ID J:53471]

Kobrynski LJ; Sousa AO; Nahmias AJ; Lee FK. 2005. Cutting edge: antibody production to pneumococcal polysaccharides requires CD1 molecules and CD8+ T cells. J Immunol 174(4):1787-90. [PubMed: 15699104]  [MGI Ref ID J:96541]

Lau LL; Spain LM. 2000. Altered aging-related thymic involution in T cell receptor transgenic, MHC-deficient, and CD4-deficient mice. Mech Ageing Dev 114(2):101-21. [PubMed: 10799708]  [MGI Ref ID J:111547]

McCarrick JW 3d; Parnes JR; Seong RH; Solter D; Knowles BB. 1993. Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2(4):183-90. [PubMed: 8364601]  [MGI Ref ID J:48775]

Morgan DJ; Nugent CT; Raveney BJ; Sherman LA. 2004. In a transgenic model of spontaneous autoimmune diabetes, expression of a protective class II MHC molecule results in thymic deletion of diabetogenic CD8+ T cells. J Immunol 172(2):1000-8. [PubMed: 14707073]  [MGI Ref ID J:100010]

Murphy TJ; Choileain NN; Zang Y; Mannick JA; Lederer JA. 2005. CD4+CD25+ regulatory T cells control innate immune reactivity after injury. J Immunol 174(5):2957-63. [PubMed: 15728508]  [MGI Ref ID J:97723]

Petrofsky M; Bermudez LE. 2005. CD4+ T cells but Not CD8+ or gammadelta+ lymphocytes are required for host protection against Mycobacterium avium infection and dissemination through the intestinal route. Infect Immun 73(5):2621-7. [PubMed: 15845464]  [MGI Ref ID J:97613]

VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356]  [MGI Ref ID J:72616]

Yang R; Murillo FM; Delannoy MJ; Blosser RL; Yutzy WH th; Uematsu S; Takeda K; Akira S; Viscidi RP; Roden RB. 2005. B lymphocyte activation by human papillomavirus-like particles directly induces Ig class switch recombination via TLR4-MyD88. J Immunol 174(12):7912-9. [PubMed: 15944297]  [MGI Ref ID J:100870]

Yang R; Wheeler CM; Chen X; Uematsu S; Takeda K; Akira S; Pastrana DV; Viscidi RP; Roden RB. 2005. Papillomavirus capsid mutation to escape dendritic cell-dependent innate immunity in cervical cancer. J Virol 79(11):6741-50. [PubMed: 15890912]  [MGI Ref ID J:98389]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Multiple; Inquire
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.