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Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 25-MAY-15


Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   12-DEC-14
Specieslaboratory mouse
Donating InvestigatorDr. David Serreze,   The Jackson Laboratory

Control Information

   001976 NOD/ShiLtJ (approximate)
  Considerations for Choosing Controls

Related Strains

Strains carrying   Cd4tm1Knw allele
002269   B6.129S6-Cd4tm1Knw/J
002268   B6;129S6-Cd4tm1Knw/J
View Strains carrying   Cd4tm1Knw     (2 strains)

View Strains carrying other alleles of Cd4     (13 strains)


Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- No similarity to the expected human disease phenotype was found. One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of these genes, but the phenotype did not resemble the disease.
Diabetes Mellitus, Insulin-Dependent; IDDM
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Okt4 Epitope Deficiency   (CD4)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype


  • immune system phenotype
  • decreased susceptibility to autoimmune diabetes
    • Cd4-deficient mice are diabetes resistant (diabetes observed in 0/18 females by 30 weeks)   (MGI Ref ID J:93553)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Cd4tm1Knw related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Cd4tm1Knw
Allele Name targeted mutation 1, Barbara B Knowles
Allele Type Targeted (Null/Knockout)
Common Name(s) CD4-; CD4D;
Mutation Made ByDr. Barbara Knowles,   Institute of Medical Biology
Strain of Origin129S/SvEv-Gpi1
ES Cell Line NameCCE/EK.CCE
ES Cell Line Strain129S/SvEv-Gpi1
Gene Symbol and Name Cd4, CD4 antigen
Chromosome 6
Gene Common Name(s) CD4mut; L3T4; Ly-4; W3/25; lymphocyte antigen 4; p55;
Molecular Note A neomycin resistance gene was inserted into exon 5. [MGI Ref ID J:48775]


Genotyping Information

Genotyping Protocols

Generic Cd4, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Additional References

Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754]  [MGI Ref ID J:93553]

Cd4tm1Knw related

Bommireddy R; Engle SJ; Ormsby I; Boivin GP; Babcock GF; Doetschman T. 2004. Elimination of both CD4(+) and CD8(+) T cells but not B cells eliminates inflammation and prolongs the survival of TGFbeta1-deficient mice. Cell Immunol 232(1-2):96-104. [PubMed: 15922720]  [MGI Ref ID J:99033]

Borchers MT; Crosby J; Justice P; Farmer S; Hines E; Lee JJ; Lee NA. 2001. Intrinsic AHR in IL-5 transgenic mice is dependent on CD4(+) cells and CD49d-mediated signaling. Am J Physiol Lung Cell Mol Physiol 281(3):L653-9. [PubMed: 11504693]  [MGI Ref ID J:132284]

Brochard V; Combadiere B; Prigent A; Laouar Y; Perrin A; Beray-Berthat V; Bonduelle O; Alvarez-Fischer D; Callebert J; Launay JM; Duyckaerts C; Flavell RA; Hirsch EC; Hunot S. 2009. Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease. J Clin Invest 119(1):182-92. [PubMed: 19104149]  [MGI Ref ID J:144702]

Chan T; Pek EA; Huth K; Ashkar AA. 2011. CD4(+) T-cells are important in regulating macrophage polarization in C57BL/6 wild-type mice. Cell Immunol 266(2):180-6. [PubMed: 21040907]  [MGI Ref ID J:167012]

Crosby JR; Shen HH; Borchers MT; Justice JP; Ansay T; Lee JJ; Lee NA. 2002. Ectopic expression of IL-5 identifies an additional CD4(+) T cell mechanism of airway eosinophil recruitment. Am J Physiol Lung Cell Mol Physiol 282(1):L99-108. [PubMed: 11741821]  [MGI Ref ID J:107840]

Ganta RR; Cheng C; Wilkerson MJ; Chapes SK. 2004. Delayed clearance of Ehrlichia chaffeensis infection in CD4+ T-cell knockout mice. Infect Immun 72(1):159-67. [PubMed: 14688093]  [MGI Ref ID J:87828]

Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754]  [MGI Ref ID J:93553]

Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890]  [MGI Ref ID J:125272]

Hancock WW; Tsai TL; Madaio MP; Gasser DL. 2003. Cutting Edge: Multiple autoimmune pathways in kd/kd mice. J Immunol 171(6):2778-81. [PubMed: 12960297]  [MGI Ref ID J:85380]

Justice JP; Crosby J; Borchers MT; Tomkinson A; Lee JJ; Lee NA. 2002. CD4(+) T cell-dependent airway mucus production occurs in response to IL-5 expression in lung. Am J Physiol Lung Cell Mol Physiol 282(5):L1066-74. [PubMed: 11943672]  [MGI Ref ID J:108354]

Kassiotis G; Bauer J; Akassoglou K; Lassmann H; Kollias G; Probert L. 1999. A tumor necrosis factor-induced model of human primary demyelinating diseases develops in immunodeficient mice. Eur J Immunol 29(3):912-7. [PubMed: 10092095]  [MGI Ref ID J:53471]

Kobrynski LJ; Sousa AO; Nahmias AJ; Lee FK. 2005. Cutting edge: antibody production to pneumococcal polysaccharides requires CD1 molecules and CD8+ T cells. J Immunol 174(4):1787-90. [PubMed: 15699104]  [MGI Ref ID J:96541]

Lau LL; Spain LM. 2000. Altered aging-related thymic involution in T cell receptor transgenic, MHC-deficient, and CD4-deficient mice. Mech Ageing Dev 114(2):101-21. [PubMed: 10799708]  [MGI Ref ID J:111547]

McCarrick JW 3rd; Parnes JR; Seong RH; Solter D; Knowles BB. 1993. Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2(4):183-90. [PubMed: 8364601]  [MGI Ref ID J:48775]

Morgan DJ; Nugent CT; Raveney BJ; Sherman LA. 2004. In a transgenic model of spontaneous autoimmune diabetes, expression of a protective class II MHC molecule results in thymic deletion of diabetogenic CD8+ T cells. J Immunol 172(2):1000-8. [PubMed: 14707073]  [MGI Ref ID J:100010]

Murphy TJ; Choileain NN; Zang Y; Mannick JA; Lederer JA. 2005. CD4+CD25+ regulatory T cells control innate immune reactivity after injury. J Immunol 174(5):2957-63. [PubMed: 15728508]  [MGI Ref ID J:97723]

Petrofsky M; Bermudez LE. 2005. CD4+ T cells but Not CD8+ or gammadelta+ lymphocytes are required for host protection against Mycobacterium avium infection and dissemination through the intestinal route. Infect Immun 73(5):2621-7. [PubMed: 15845464]  [MGI Ref ID J:97613]

Russell MS; Dudani R; Krishnan L; Sad S. 2009. IFN-gamma expressed by T cells regulates the persistence of antigen presentation by limiting the survival of dendritic cells. J Immunol 183(12):7710-8. [PubMed: 19923462]  [MGI Ref ID J:157498]

Stolp J; Chen YG; Cox SL; Henck V; Zhang W; Tsaih SW; Chapman H; Stearns T; Serreze DV; Silveira PA. 2012. Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice. J Immunol 189(3):1406-17. [PubMed: 22732593]  [MGI Ref ID J:189784]

VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356]  [MGI Ref ID J:72616]

Venet F; Chung CS; Huang X; Lomas-Neira J; Chen Y; Ayala A. 2009. Lymphocytes in the development of lung inflammation: a role for regulatory CD4+ T cells in indirect pulmonary lung injury. J Immunol 183(5):3472-80. [PubMed: 19641139]  [MGI Ref ID J:151875]

Vieyra M; Leisman S; Raedler H; Kwan WH; Yang M; Strainic MG; Medof ME; Heeger PS. 2011. Complement regulates CD4 T-cell help to CD8 T cells required for murine allograft rejection. Am J Pathol 179(2):766-74. [PubMed: 21704012]  [MGI Ref ID J:174402]

Yang R; Murillo FM; Delannoy MJ; Blosser RL; Yutzy WH 4th; Uematsu S; Takeda K; Akira S; Viscidi RP; Roden RB. 2005. B lymphocyte activation by human papillomavirus-like particles directly induces Ig class switch recombination via TLR4-MyD88. J Immunol 174(12):7912-9. [PubMed: 15944297]  [MGI Ref ID J:100870]

Yang R; Wheeler CM; Chen X; Uematsu S; Takeda K; Akira S; Pastrana DV; Viscidi RP; Roden RB. 2005. Papillomavirus capsid mutation to escape dendritic cell-dependent innate immunity in cervical cancer. J Virol 79(11):6741-50. [PubMed: 15890912]  [MGI Ref ID J:98389]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   12-DEC-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 25-MAY-15

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Cd4tm1Knw  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Cd4tm1Knw x Homozygous for Cd4tm1Knw  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Cd4tm1Knw  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Cd4tm1Knw x Homozygous for Cd4tm1Knw  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

General Supply Notes

Control Information

   001976 NOD/ShiLtJ (approximate)
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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