Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.D2-Vac14ingls/J    (Changed: 22-JUN-09 ) B6.D2-ingls/J    (Changed: 14-JAN-09 ) Type Congenic; Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6J Donor Strain DBA/2J H2 Haplotype b Generation N23F1pN1 Generation Definitions

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the recessive Vac14^ingls mutation are significantly smaller than their littermates, and their coat color is dilute with white underfur both dorsally and ventrally (Sweet 1991; Samples 2003). Most develop hydrocephalus with onset by one week of age. Homozygotes are weak and uncoordinated and have difficulty righting themselves. They fail to gain weight during the second week of life, and most die by three weeks of age. (Bronson et al. 2003) Length of survival appears to correlate with the severity of hydrocephalus, which varies from absent to severe; however, all homozygotes die by four weeks, whether or not they are hydrocephalic (Samples et al. 2003).

Hydrocephalus affects the lateral ventricles most severely; serial sections reveal an intact cerebral aqueduct. Diffuse astrocytic hypertrophy and hyperplasia are observed in brains and spinal cords of mutant mice. A few mutants develop status spongiosis, and a few exhibit gliosis alone. Astrocytes cultured from affected mice stop dividing within three weeks, but survive for up to 12 weeks. They do not develop into tumors when implanted into the brains of normal mice. (Bronson et al. 2003)

Development
ingls arose spontaneously in a breeding colony of the inbred strain DBA/2J at The Jackson Laboratory in 1991. It has been backcrossed onto C57BL/6J and is maintained by crossing hosts of homozygous ovarian transplants to C57BL/6J males, then intercrossing the resultant obligate heterozygotes. In October 2003 this strain had reached generation N19F1.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Vac14^ingls/Vac14^ingls

        B6.D2-Vac14^ingls/GrsrJ

• mortality/aging
• complete postnatal lethality
  • die by 22 days of age   (MGI Ref ID J:81516)
  • mice die between P1 and P21   (MGI Ref ID J:142703)
• nervous system phenotype
• enlarged brain ventricle
  • enlarged   (MGI Ref ID J:142703)
• gliosis
  • astrocytic hypertrophy and hyperplasia in brain stem and spinal cord and to a much lesser extent in the cerebral cortex, thalamus, and midbrain, within the first weeks of life with most homozygotes developing hydrocephalus and a few developing status spongiosis   (MGI Ref ID J:81516)
• • astrocytosis   (MGI Ref ID J:142703)
    • astrocytes from affected mice cease dividing shortly after being put in culture   (MGI Ref ID J:81516)
• hydroencephaly
  • often by 1 week of age and usually severe with lateral ventricles most severely affected, the third ventricle less severely affected, and the fourth ventricle normal   (MGI Ref ID J:81516)
  • severe at P16   (MGI Ref ID J:142703)
• spongiform encephalopathy   (MGI Ref ID J:81516)
  • in several regions of the brain including thalamus, brain stem and cerebellar nucleus   (MGI Ref ID J:142703)
  • however, spinal motor neurons and sensory neurons remain intact   (MGI Ref ID J:142703)
• behavior/neurological phenotype
• abnormal motor coordination/ balance
  • at P14, mice exhibit impaired motor function   (MGI Ref ID J:142703)
• • ataxia   (MGI Ref ID J:81516)
• tremors
  • at P14   (MGI Ref ID J:142703)
• growth/size phenotype
• decreased body size   (MGI Ref ID J:142703)
• pigmentation phenotype
• diluted coat color   (MGI Ref ID J:142703)
• integument phenotype
• diluted coat color   (MGI Ref ID J:142703)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Vac14^ingls related

Neurobiology Research
Astrocyte Defects
      astrocytosis

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Vac14ingls
Allele Name		infantile gliosis
Allele Type		Spontaneous
Common Name(s)		Vac14L156R; ingls;
Strain of Origin		DBA/2J
Gene Symbol and Name		Vac14, Vac14 homolog (S. cerevisiae)
Chromosome		8
Gene Common Name(s)		AA959718; ArPIKfyve; BC032215; D8Wsu151e; DNA segment, Chr 8, Wayne State University 151, expressed; MGC:38230; TAX1BP2; TRX; Tax1 (human T-cell leukemia virus type I) binding protein 2; Tax1bp2; cDNA sequence BC032215; expressed sequence AA959718; infantile gliosis; ingls;
General Note		This spontaneous mutation arose in 1991 at The Jackson Laboratory.
Molecular Note		Exon 4 contains a thymidine to guanosine transition at position 467 (c.467T>G) that results in an amino acid substitution of arginine for leucine at position 156 (L156R). This allele is hypomorphic. [MGI Ref ID J:142703]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bronson R; Perides G; Sweet H; Ward-Bailey PF; Davisson M. 2003. Infantile gliosis (ingls) a new spontaneous mutation in the mouse. Mouse Mutant Resources Web Site, The Jackson Laboratory, Bar Harbor, Maine MGI Direct Data Submission :.  [MGI Ref ID J:81516]

Additional References

Vac14^ingls related

Ferguson CJ; Lenk GM; Meisler MH. 2009. Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2. Hum Mol Genet 18(24):4868-4878. [PubMed: 19793721]  [MGI Ref ID J:154296]

Jin N; Chow CY; Liu L; Zolov SN; Bronson R; Davisson M; Petersen JL; Zhang Y; Park S; Duex JE; Goldowitz D; Meisler MH; Weisman LS. 2008. VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse. EMBO J 27(24):3221-34. [PubMed: 19037259]  [MGI Ref ID J:142703]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• View the complete collection of spontaneous mutants in the Mouse Mutant Resource.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.