Strain Name:

B6.D2-Vac14ingls/GrsrJ

Stock Number:

003095

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.D2-Vac14ingls/J    (Changed: 22-JUN-09 )
B6.D2-ingls/J    (Changed: 14-JAN-09 )
Type Congenic; Mutant Stock; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain DBA/2J
H2 Haplotypeb

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the recessive Vac14ingls mutation are significantly smaller than their littermates, and their coat color is dilute with white underfur both dorsally and ventrally (Sweet 1991; Samples 2003). Most develop hydrocephalus with onset by one week of age. Homozygotes are weak and uncoordinated and have difficulty righting themselves. They fail to gain weight during the second week of life, and most die by three weeks of age. (Bronson et al. 2003) Length of survival appears to correlate with the severity of hydrocephalus, which varies from absent to severe; however, all homozygotes die by four weeks, whether or not they are hydrocephalic (Samples et al. 2003).

Hydrocephalus affects the lateral ventricles most severely; serial sections reveal an intact cerebral aqueduct. Diffuse astrocytic hypertrophy and hyperplasia are observed in brains and spinal cords of mutant mice. A few mutants develop status spongiosis, and a few exhibit gliosis alone. Astrocytes cultured from affected mice stop dividing within three weeks, but survive for up to 12 weeks. They do not develop into tumors when implanted into the brains of normal mice. (Bronson et al. 2003)

Development
ingls arose spontaneously in a breeding colony of the inbred strain DBA/2J at The Jackson Laboratory in 1991. It has been backcrossed onto C57BL/6J and is maintained by crossing hosts of homozygous ovarian transplants to C57BL/6J males, then intercrossing the resultant obligate heterozygotes. In October 2003 this strain had reached generation N19F1.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Vac14ingls/Vac14ingls

        B6.D2-Vac14ingls/GrsrJ
  • mortality/aging
  • complete postnatal lethality
    • die by 22 days of age   (MGI Ref ID J:81516)
    • mice die between P1 and P21   (MGI Ref ID J:142703)
  • nervous system phenotype
  • enlarged brain ventricles
  • gliosis
    • astrocytic hypertrophy and hyperplasia in brain stem and spinal cord and to a much lesser extent in the cerebral cortex, thalamus, and midbrain, within the first weeks of life with most homozygotes developing hydrocephalus and a few developing status spongiosis   (MGI Ref ID J:81516)
    • astrocytosis   (MGI Ref ID J:142703)
      • astrocytes from affected mice cease dividing shortly after being put in culture   (MGI Ref ID J:81516)
  • hydroencephaly
    • often by 1 week of age and usually severe with lateral ventricles most severely affected, the third ventricle less severely affected, and the fourth ventricle normal   (MGI Ref ID J:81516)
    • severe at P16   (MGI Ref ID J:142703)
  • spongiform encephalopathy   (MGI Ref ID J:81516)
    • in several regions of the brain including thalamus, brain stem and cerebellar nucleus   (MGI Ref ID J:142703)
    • however, spinal motor neurons and sensory neurons remain intact   (MGI Ref ID J:142703)
  • behavior/neurological phenotype
  • abnormal motor coordination/ balance
    • at P14, mice exhibit impaired motor function   (MGI Ref ID J:142703)
  • tremors
  • growth/size/body phenotype
  • decreased body size   (MGI Ref ID J:142703)
  • pigmentation phenotype
  • diluted coat color   (MGI Ref ID J:142703)
  • integument phenotype
  • diluted coat color   (MGI Ref ID J:142703)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Vac14ingls related

Neurobiology Research
Astrocyte Defects
      astrocytosis

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Vac14ingls
Allele Name infantile gliosis
Allele Type Spontaneous (Hypomorph)
Common Name(s) Vac14L156R; ingls;
Strain of OriginDBA/2J
Gene Symbol and Name Vac14, Vac14 homolog (S. cerevisiae)
Chromosome 8
Gene Common Name(s) AA959718; ArPIKfyve; BC032215; D8Wsu151e; DNA segment, Chr 8, Wayne State University 151, expressed; MGC:38230; TAX1BP2; TRX; Tax1 (human T-cell leukemia virus type I) binding protein 2; Tax1bp2; cDNA sequence BC032215; expressed sequence AA959718; infantile gliosis; ingls;
General Note This spontaneous mutation arose in 1991 at The Jackson Laboratory.
Molecular Note Exon 4 contains a thymidine to guanosine transition at position 467 (c.467T>G) that results in an amino acid substitution of arginine for leucine at position 156 (L156R). This allele is hypomorphic. [MGI Ref ID J:142703]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bronson R; Perides G; Sweet H; Ward-Bailey PF; Davisson M. 2003. Infantile gliosis (ingls) a new spontaneous mutation in the mouse. Mouse Mutant Resources Web Site, The Jackson Laboratory, Bar Harbor, Maine MGI Direct Data Submission :.  [MGI Ref ID J:81516]

Additional References

Vac14ingls related

Ferguson CJ; Lenk GM; Meisler MH. 2009. Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2. Hum Mol Genet 18(24):4868-4878. [PubMed: 19793721]  [MGI Ref ID J:154296]

Jin N; Chow CY; Liu L; Zolov SN; Bronson R; Davisson M; Petersen JL; Zhang Y; Park S; Duex JE; Goldowitz D; Meisler MH; Weisman LS. 2008. VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse. EMBO J 27(24):3221-34. [PubMed: 19037259]  [MGI Ref ID J:142703]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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