Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation ?pN1   Donating Investigator Caroline Damsky,   Univ of California, San Francisco

Description
Mice homozygous for the Itgb1^tmlLscd targeted mutation die during early postimplantation development. Beta1-null embryos form normal-looking blastocysts and initiate implantation at E4.5. By E5.5, Beta1-null embryos degenerate extensively. The inner cell mass region of blastocyst outgrowths show highly retarded growth and defective extra-embryonic endoderm morphogenesis and migration.

Control Information

		Wildtype mice from the colony may be used as controls. This strain is a mixture of C57BL/6 and 129 inbred strains and the Swiss Webster Black random bred strain.
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Itgb1

004605

B6;129-Itgb1tm1Efu/J

View Strains carrying other alleles of Itgb1     (1 strain)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Itgb1^tm1Lscd related

Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
Postnatal Mortality

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Itgb1tm1Lscd
Allele Name		targeted mutation 1, Caroline H Damsky
Allele Type		Targeted (knock-out)
Common Name(s)		Itgbeta1-; beta1-null;
Mutation Made By		Laurie Stephens,   Univ of California, San Francisco
Strain of Origin		129X1/SvJ
ES Cell Line Name		JM-1
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Itgb1, integrin beta 1 (fibronectin receptor beta)
Chromosome		8
Gene Common Name(s)		4633401G24Rik; AA409975; AA960159; CD29; FNRB; Fnrb; GPIIA; MDF2; MSK12; RIKEN cDNA 4633401G24 gene; VLA-BETA; VLAB; beta1 integrin; expressed sequence AA409975; expressed sequence AA960159; fibronectin receptor beta (integrin);
Molecular Note		A promoterless neomycin resistance gene fusion was inserted in-frame into exon 2 of the gene. [MGI Ref ID J:28266]

Genotyping

Genotyping Information

Genotyping Protocols

Itgb1^tm1Lscd, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Stephens LE; Sutherland AE; Klimanskaya IV; Andrieux A; Meneses J; Pedersen RA; Damsky CH. 1995. Deletion of beta 1 integrins in mice results in inner cell mass failure and peri-implantation lethality. Genes Dev 9(15):1883-95. [PubMed: 7544312]  [MGI Ref ID J:28266]

Additional References

Itgb1^tm1Lscd related

Blaess S; Graus-Porta D; Belvindrah R; Radakovits R; Pons S; Littlewood-Evans A; Senften M; Guo H; Li Y; Miner JH; Reichardt LF; Muller U. 2004. Beta1-integrins are critical for cerebellar granule cell precursor proliferation. J Neurosci 24(13):3402-12. [PubMed: 15056720]  [MGI Ref ID J:96910]

Breau MA; Pietri T; Eder O; Blanche M; Brakebusch C; Fassler R; Thiery JP; Dufour S. 2006. Lack of {beta}1 integrins in enteric neural crest cells leads to a Hirschsprung-like phenotype. Development 133(9):1725-34. [PubMed: 16571628]  [MGI Ref ID J:108439]

Feltri ML; Graus Porta D; Previtali SC; Nodari A; Migliavacca B; Cassetti A; Littlewood-Evans A; Reichardt LF; Messing A; Quattrini A; Mueller U; Wrabetz L. 2002. Conditional disruption of beta 1 integrin in Schwann cells impedes interactions with axons. J Cell Biol 156(1):199-209. [PubMed: 11777940]  [MGI Ref ID J:73874]

Graus-Porta D; Blaess S; Senften M; Littlewood-Evans A; Damsky C; Huang Z; Orban P; Klein R; Schittny JC; Muller U. 2001. Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex. Neuron 31(3):367-79. [PubMed: 11516395]  [MGI Ref ID J:71122]

Huang Z; Shimazu K; Woo NH; Zang K; Muller U; Lu B; Reichardt LF. 2006. Distinct roles of the beta 1-class integrins at the developing and the mature hippocampal excitatory synapse. J Neurosci 26(43):11208-19. [PubMed: 17065460]  [MGI Ref ID J:114964]

Pietri T; Eder O; Breau MA; Topilko P; Blanche M; Brakebusch C; Fassler R; Thiery JP; Dufour S. 2004. Conditional beta1-integrin gene deletion in neural crest cells causes severe developmental alterations of the peripheral nervous system. Development 131(16):3871-83. [PubMed: 15253938]  [MGI Ref ID J:92060]

Schwander M; Leu M; Stumm M; Dorchies OM; Ruegg UT; Schittny J; Muller U. 2003. Beta1 integrins regulate myoblast fusion and sarcomere assembly. Dev Cell 4(5):673-85. [PubMed: 12737803]  [MGI Ref ID J:129360]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

		Wildtype mice from the colony may be used as controls. This strain is a mixture of C57BL/6 and 129 inbred strains and the Swiss Webster Black random bred strain.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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