Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1 Generation Definitions   Donating Investigator Dr. Caroline Damsky,   Univ of California, San Francisco

Description
Mice homozygous for the Itgb1^tmlLscd targeted mutation die during early postimplantation development. Beta1-null embryos form normal-looking blastocysts and initiate implantation at E4.5. By E5.5, Beta1-null embryos degenerate extensively. The inner cell mass region of blastocyst outgrowths show highly retarded growth and defective extra-embryonic endoderm morphogenesis and migration.

Control Information

	Control
	See control note:	Wildtype mice from the colony may be used as controls. This strain is a mixture of C57BL/6 and 129 inbred strains and the Swiss Webster Black random bred strain.
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Itgb1

004605

B6;129-Itgb1tm1Efu/J

View Strains carrying other alleles of Itgb1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Itgb1^tm1Lscd/Itgb1^tm1Lscd

        involves: 129X1/SvJ * Black Swiss

• mortality/aging
• complete embryonic lethality between implantation and somite formation
  • lethality occurs between E4.5 and E5.5 due to inner cell mass defects   (MGI Ref ID J:28266)
• embryogenesis phenotype
• abnormal blastocoele morphology
  • E4.5 embryos have collapsed blastocoeles   (MGI Ref ID J:28266)
• abnormal inner cell mass morphology
  • isolated inner cell masses attach, spread and differentiate very poorly on a fibronectin substrate   (MGI Ref ID J:28266)
• • inner cell mass degeneration
    • inner cell mass of cultured blastocytes have highly restricted growth and defective endoderm morphogenesis and migration   (MGI Ref ID J:28266)
    • 48 hours of blastocyst culture in serum leads to inner cell masses that are significantly smaller and appear disorganized compared to controls   (MGI Ref ID J:28266)
• abnormal trophoblast layer morphology
  • the trophoblasts of E4.5 embryos fails to extensively invade the maternal decidua   (MGI Ref ID J:28266)
  • trophoblast outgrowth from cultured blastocytes is defective when laminin is provided as a substrate   (MGI Ref ID J:28266)
• abnormal visceral endoderm morphology
  • the monolayer of visceral endoderm is not formed in these inner cell masses despite the presence of laminin-positive cell clusters   (MGI Ref ID J:28266)
  • only 3 of 17 inner masses demonstrate any morphogenesis of the endoderm under this conditions compared to the 100% morphogenesis that occurs to controls   (MGI Ref ID J:28266)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Itgb1^tm1Lscd related

Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
Postnatal Lethality

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Itgb1tm1Lscd
Allele Name		targeted mutation 1, Caroline H Damsky
Allele Type		Targeted (knock-out)
Common Name(s)		Itgbeta1-; beta1-null;
Mutation Made By		Dr. Laurie Stephens,   Univ of California, San Francisco
Strain of Origin		129X1/SvJ
ES Cell Line Name		JM-1
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Itgb1, integrin beta 1 (fibronectin receptor beta)
Chromosome		8
Gene Common Name(s)		4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; FNRB; Fnrb; GPIIA; Gm9863; MDF2; MSK12; RIKEN cDNA 4633401G24 gene; VLA-BETA; VLAB; beta1 integrin; expressed sequence AA409975; expressed sequence AA960159; fibronectin receptor beta (integrin); predicted gene 9863; predicted gene, ENSMUSG00000051907;
General Note		Phenotypic Similarity to Human Syndrome: Hirschsprung disease in mice heterozygous for Itgb1tm1Lscd and Itgb1tm1Ref and hemizygous for Tg(PLAT-cre)116Sdu (J:108439)
Molecular Note		A promoterless neomycin resistance gene fusion was inserted in-frame into exon 2 of the gene. [MGI Ref ID J:28266]

Genotyping

Genotyping Information

Genotyping Protocols

Itgb1^tm1Lscd, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Stephens LE; Sutherland AE; Klimanskaya IV; Andrieux A; Meneses J; Pedersen RA; Damsky CH. 1995. Deletion of beta 1 integrins in mice results in inner cell mass failure and peri-implantation lethality. Genes Dev 9(15):1883-95. [PubMed: 7544312]  [MGI Ref ID J:28266]

Additional References

Itgb1^tm1Lscd related

Barros CS; Nguyen T; Spencer KS; Nishiyama A; Colognato H; Muller U. 2009. Beta1 integrins are required for normal CNS myelination and promote AKT-dependent myelin outgrowth. Development 136(16):2717-24. [PubMed: 19633169]  [MGI Ref ID J:152914]

Blaess S; Graus-Porta D; Belvindrah R; Radakovits R; Pons S; Littlewood-Evans A; Senften M; Guo H; Li Y; Miner JH; Reichardt LF; Muller U. 2004. Beta1-integrins are critical for cerebellar granule cell precursor proliferation. J Neurosci 24(13):3402-12. [PubMed: 15056720]  [MGI Ref ID J:96910]

Breau MA; Pietri T; Eder O; Blanche M; Brakebusch C; Fassler R; Thiery JP; Dufour S. 2006. Lack of {beta}1 integrins in enteric neural crest cells leads to a Hirschsprung-like phenotype. Development 133(9):1725-34. [PubMed: 16571628]  [MGI Ref ID J:108439]

Feltri ML; Graus Porta D; Previtali SC; Nodari A; Migliavacca B; Cassetti A; Littlewood-Evans A; Reichardt LF; Messing A; Quattrini A; Mueller U; Wrabetz L. 2002. Conditional disruption of beta 1 integrin in Schwann cells impedes interactions with axons. J Cell Biol 156(1):199-209. [PubMed: 11777940]  [MGI Ref ID J:73874]

Graus-Porta D; Blaess S; Senften M; Littlewood-Evans A; Damsky C; Huang Z; Orban P; Klein R; Schittny JC; Muller U. 2001. Beta1-class integrins regulate the development of laminae and folia in the cerebral and cerebellar cortex. Neuron 31(3):367-79. [PubMed: 11516395]  [MGI Ref ID J:71122]

Huang Z; Shimazu K; Woo NH; Zang K; Muller U; Lu B; Reichardt LF. 2006. Distinct roles of the beta 1-class integrins at the developing and the mature hippocampal excitatory synapse. J Neurosci 26(43):11208-19. [PubMed: 17065460]  [MGI Ref ID J:114964]

Pietri T; Eder O; Breau MA; Topilko P; Blanche M; Brakebusch C; Fassler R; Thiery JP; Dufour S. 2004. Conditional beta1-integrin gene deletion in neural crest cells causes severe developmental alterations of the peripheral nervous system. Development 131(16):3871-83. [PubMed: 15253938]  [MGI Ref ID J:92060]

Schwander M; Leu M; Stumm M; Dorchies OM; Ruegg UT; Schittny J; Muller U. 2003. Beta1 integrins regulate myoblast fusion and sarcomere assembly. Dev Cell 4(5):673-85. [PubMed: 12737803]  [MGI Ref ID J:129360]

Schwander M; Shirasaki R; Pfaff SL; Muller U. 2004. Beta1 integrins in muscle, but not in motor neurons, are required for skeletal muscle innervation. J Neurosci 24(37):8181-91. [PubMed: 15371519]  [MGI Ref ID J:146417]

Turlo KA; Gallaher SD; Vora R; Laski FA; Iruela-Arispe ML. 2010. When cre-mediated recombination in mice does not result in protein loss. Genetics 186(3):959-67. [PubMed: 20813881]  [MGI Ref ID J:165707]

Zovein AC; Luque A; Turlo KA; Hofmann JJ; Yee KM; Becker MS; Fassler R; Mellman I; Lane TF; Iruela-Arispe ML. 2010. Beta1 integrin establishes endothelial cell polarity and arteriolar lumen formation via a Par3-dependent mechanism. Dev Cell 18(1):39-51. [PubMed: 20152176]  [MGI Ref ID J:157920]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	See control note:	Wildtype mice from the colony may be used as controls. This strain is a mixture of C57BL/6 and 129 inbred strains and the Swiss Webster Black random bred strain.
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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