Strain Name:

B6.129S2-Ntrk2tm1Bbd/J

Stock Number:

003098

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S2 via D3 ES cell line
GenerationN7
Generation Definitions
 
Donating Investigator The Jackson Laboratory,  

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for this Ntrk2tm targeted mutation (commonly referred to as trkB) die by 2-3 weeks of age. Homozygous mutant mice show a complete lack of nodose, vestibular, and cochlear neurons. In addition, mice display CNS deficits including cell death in dentate gyrus, cortical layers II-III and V-VI, striatum and thalamus.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Ntrk2tm1Bbd allele
002544   B6;129S2-Ntrk2tm1Bbd/J
View Strains carrying   Ntrk2tm1Bbd     (1 strain)

Strains carrying other alleles of Ntrk2
022363   B6.129P2(SJL)-Ntrk2tm1Ddg/J
View Strains carrying other alleles of Ntrk2     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Obesity, Hyperphagia, and Developmental Delay   (NTRK2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ntrk2tm1Bbd/Ntrk2tm1Bbd

        involves: 129S2/SvPas * C57BL/6
  • mortality/aging
  • partial neonatal lethality
    • most die by P1, although a few survive into the first week   (MGI Ref ID J:56847)
  • behavior/neurological phenotype
  • abnormal vibrissae reflex
    • unresponsive to tactile stimuli in vibrissae area   (MGI Ref ID J:56847)
  • no suckling reflex
    • do not orient toward a stimulus (stroking under the chin) and do not open mouth except for an occasional gasp   (MGI Ref ID J:56847)
  • no swallowing reflex
    • homozygotes inhale milk instead of swallowing it when manually fed   (MGI Ref ID J:56847)
  • nervous system phenotype
  • abnormal cochlear ganglion morphology
    • at P1, cochlear ganglia display areas of reduced cell density and large extracellular spaces, albeit to a lesser extent than vestibular ganglia   (MGI Ref ID J:29245)
    • small cochlear ganglion
      • at P1, a 11% and 15% reduction in cochlear ganglion size and neuron number is, respectively, observed   (MGI Ref ID J:29245)
      • small-sized type II neurons (innervating OHCs) are preferentially lost (77% reduction), whereas larged-sized type I neurons (innervating IHCs) remain unaffected   (MGI Ref ID J:29245)
      • no significant changes in cochlear ganglion size or neuron number are noted at E14.5 or E18.5   (MGI Ref ID J:29245)
  • abnormal sensory neuron innervation pattern
    • at P1, homozygotes display deficient innervation in the vestibular and cochlear sensory epithelia   (MGI Ref ID J:29245)
    • in the utricular macula nerve fibers have reached the base of the hair cells, but are degenerating and retracting from their targets   (MGI Ref ID J:29245)
    • no innervation is observed in the sensory epithelia of the ampullary cristae   (MGI Ref ID J:29245)
    • at P1, vestibular nerves lack membrane vesicles and contain residual synaptic plates at their nerve endings; degenerating nerve fibers, empty perineural sheets and lack of myelination are observed   (MGI Ref ID J:29245)
    • as shown at E16.5, some vestibular and cochlear fibres initially reach their peripheral targets but fail to maintain innervation and thus degenerate   (MGI Ref ID J:29245)
    • abnormal cochlear OHC afferent innervation pattern
      • at P1, homozygotes fail to innervate the OHCs of the cochlea   (MGI Ref ID J:29245)
      • the sensory epithelium containing OHCs is generally devoid of nerve fibers, although retracting neurites with residual synaptic contacts are occasionally detected   (MGI Ref ID J:29245)
      • in contrast, the innervation pattern of IHCs appears normal   (MGI Ref ID J:29245)
  • abnormal vestibular ganglion morphology
    • at P1, vestibular ganglia display areas of reduced cell density and large extracellular spaces   (MGI Ref ID J:29245)
    • at this stage, cells with nuclei surrounded by a very small amount of cytoplasm are abundant   (MGI Ref ID J:29245)
    • small vestibular ganglion
      • at E18.5, a 38% reduction in vestibular neuron number is observed   (MGI Ref ID J:29245)
      • by P1, a 57% reduction in vestibular ganglion size and neuron number is observed   (MGI Ref ID J:29245)
      • however, no significant changes in vestibular ganglion size or neuron number are noted at E14.5   (MGI Ref ID J:29245)
  • decreased motor neuron number
    • reduction in motor neuron number in lumbar region only (L2-L5)   (MGI Ref ID J:56847)
  • small dorsal root ganglion
    • about 30% reduction in the number of neurons in the dorsal root ganglia from thoracic level 12 to the lumbar level 3 regions of the spinal cord   (MGI Ref ID J:56847)
  • small facial motor nucleus
    • up to 70% reduction in density of facial motor nucleus   (MGI Ref ID J:56847)
  • small trigeminal ganglion
    • about 40% reduction in the number of ganglion neurons, with the largest decrease in the anterior half of the ganglion, however size of neurons is normal   (MGI Ref ID J:56847)
  • hearing/vestibular/ear phenotype
  • abnormal cochlear OHC afferent innervation pattern
    • at P1, homozygotes fail to innervate the OHCs of the cochlea   (MGI Ref ID J:29245)
    • the sensory epithelium containing OHCs is generally devoid of nerve fibers, although retracting neurites with residual synaptic contacts are occasionally detected   (MGI Ref ID J:29245)
    • in contrast, the innervation pattern of IHCs appears normal   (MGI Ref ID J:29245)
  • abnormal crista ampullaris neuroepithelium morphology
    • at P1, no innervation is observed in the sensory epithelia of the ampullary cristae   (MGI Ref ID J:29245)
  • abnormal utricular macula morphology
    • at P1, nerve fibers have reached the base of hair cells in the utricular maculae but appear to degenerate and retract from their targets   (MGI Ref ID J:29245)

Ntrk2tm1Bbd/Ntrk2tm1Bbd

        involves: 129S2/SvPas
  • mortality/aging
  • complete postnatal lethality
    • average lifespan is 4.48 days (all survive past the first day), however those that survive past 5 days, die at an average age of 15.5 days and the maximum life span is 21 days   (MGI Ref ID J:104489)
  • nervous system phenotype
  • abnormal Purkinje cell dendrite morphology
    • dendritic trees of Purkinje cells are reduced by 22% in length   (MGI Ref ID J:49472)
  • abnormal barrel cortex morphology
    • delay in barrel formation through P6, but the deficiency disappears by P8   (MGI Ref ID J:103935)
  • abnormal cerebellum development
    • defects in cerebellar development, with the intercrural, prepyramidal, and uvular fissures shallower at P12   (MGI Ref ID J:49472)
  • abnormal somatic nervous system morphology   (MGI Ref ID J:104489)
    • abnormal sensory neuron innervation pattern
      • severe loss of radial fibers in the apical region of the cochlea that becomes less severe toward the base   (MGI Ref ID J:104489)
      • innervation into layer IV of the cortex by thalamic axons is uniform (rather than segregated into individual barrels) and exhibits a reduced branching within this layer compared to controls at P6, but becomes less apparent by P8   (MGI Ref ID J:103935)
    • small L4 dorsal root ganglion
      • 27% reduction of neuron number in L4 dorsal root ganglia   (MGI Ref ID J:104489)
    • small cochlear ganglion
      • 13% reduction of neuron number in cochlear ganglia   (MGI Ref ID J:104489)
    • small geniculate ganglion
      • 95% reduction of neuron number in geniculate ganglia   (MGI Ref ID J:104489)
    • small nodose ganglion
      • 66% reduction of neuron number in nodose/petrosal ganglia   (MGI Ref ID J:104489)
      • 87% of nodose neurons are lost by P0   (MGI Ref ID J:49472)
    • small petrosal ganglion
      • 66% reduction of neuron number in nodose/petrosal ganglia   (MGI Ref ID J:104489)
    • small trigeminal ganglion
      • 27% reduction of neuron number in trigeminal ganglia   (MGI Ref ID J:104489)
    • small vestibular ganglion
      • 83% reduction of neuron number in vestibular ganglia   (MGI Ref ID J:104489)
      • 60% of vestibular neurons are lost by birth   (MGI Ref ID J:49472)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Ntrk2tm1Bbd related

Cancer Research
Growth Factors/Receptors/Cytokines

Neurobiology Research
Hearing Defects
Neurotrophic Factor Defects
Receptor Defects

Sensorineural Research
Hearing Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ntrk2tm1Bbd
Allele Name targeted mutation 1, Mariano Barbacid
Allele Type Targeted (knock-out)
Common Name(s) gp145trkB; trkB-; trkBTK(-); trkBnull;
Mutation Made ByDr. Mariano Barbacid,   Centro Nacional de Investigaciones Oncol
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Ntrk2, neurotrophic tyrosine kinase, receptor, type 2
Chromosome 13
Gene Common Name(s) AI848316; C030027L06Rik; GP145-TrkB; RATTRKB1; RIKEN cDNA C030027L06 gene; TRKB; TRKB1; Tkrb; expressed sequence AI848316; trk-B; tyrosine protein kinase receptor;
Molecular Note Gene disruption by insertion of a PGK-neomycin cassette into exon K2, which encodes subdomains III - V of the tyrosine kinase domain. [MGI Ref ID J:56847]

Genotyping

Genotyping Information

Genotyping Protocols

NEOTD (Generic Neo), Standard PCR
Ntrk2tm1Bbd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Klein R; Smeyne RJ; Wurst W; Long LK; Auerbach BA; Joyner AL; Barbacid M. 1993. Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death. Cell 75(1):113-22. [PubMed: 8402890]  [MGI Ref ID J:56847]

Additional References

Fagan AM; Zhang H; Landis S; Smeyne RJ; Silos-Santiago I; Barbacid M. 1996. TrkA, but not TrkC, receptors are essential for survival of sympathetic neurons in vivo. J Neurosci 16(19):6208-18. [PubMed: 8815902]  [MGI Ref ID J:35563]

Minichiello L; Piehl F; Vazquez E; Schimmang T; Hokfelt T; Represa J; Klein R. 1995. Differential effects of combined trk receptor mutations on dorsal root ganglion and inner ear sensory neurons. Development 121(12):4067-75. [PubMed: 8575307]  [MGI Ref ID J:30344]

Pollock GS; Robichon R; Boyd KA; Kerkel KA; Kramer M; Lyles J; Ambalavanar R; Khan A; Kaplan DR; Williams RW; Frost DO. 2003. TrkB receptor signaling regulates developmental death dynamics, but not final number, of retinal ganglion cells. J Neurosci 23(31):10137-45. [PubMed: 14602830]  [MGI Ref ID J:88208]

Schimmang T; Minichiello L; Vazquez E; San Jose I; Giraldez F; Klein R; Represa J. 1995. Developing inner ear sensory neurons require TrkB and TrkC receptors for innervation of their peripheral targets. Development 121(10):3381-91. [PubMed: 7588071]  [MGI Ref ID J:29245]

Schimmang T; Tan J; Muller M; Zimmermann U; Rohbock K; Kopschall I; Limberger A; Minichiello L; Knipper M. 2003. Lack of Bdnf and TrkB signalling in the postnatal cochlea leads to a spatial reshaping of innervation along the tonotopic axis and hearing loss. Development 130(19):4741-50. [PubMed: 12925599]  [MGI Ref ID J:84871]

Ntrk2tm1Bbd related

Alcantara S; Frisen J; del Rio JA; Soriano E; Barbacid M; Silos-Santiago I. 1997. TrkB signaling is required for postnatal survival of CNS neurons and protects hippocampal and motor neurons from axotomy-induced cell death. J Neurosci 17(10):3623-33. [PubMed: 9133385]  [MGI Ref ID J:79303]

Allen GC; Qu X; Earnest DJ. 2005. TrkB-deficient mice show diminished phase shifts of the circadian activity rhythm in response to light. Neurosci Lett 378(3):150-5. [PubMed: 15781149]  [MGI Ref ID J:104930]

Bonanomi D; Chivatakarn O; Bai G; Abdesselem H; Lettieri K; Marquardt T; Pierchala BA; Pfaff SL. 2012. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell 148(3):568-82. [PubMed: 22304922]  [MGI Ref ID J:180801]

Calella AM; Nerlov C; Lopez RG; Sciarretta C; von Bohlen und Halbach O; Bereshchenko O; Minichiello L. 2007. Neurotrophin/Trk receptor signaling mediates C/EBPalpha, -beta and NeuroD recruitment to immediate-early gene promoters in neuronal cells and requires C/EBPs to induce immediate-early gene transcription. Neural Dev 2:4. [PubMed: 17254333]  [MGI Ref ID J:160655]

Carmona MA; Martinez A; Soler A; Blasi J; Soriano E; Aguado F. 2003. Ca(2+)-evoked synaptic transmission and neurotransmitter receptor levels are impaired in the forebrain of trkb (-/-) mice. Mol Cell Neurosci 22(2):210-26. [PubMed: 12676531]  [MGI Ref ID J:82756]

Carmona MA; Pozas E; Martinez A; Espinosa-Parrilla JF; Soriano E; Aguado F. 2006. Age-dependent spontaneous hyperexcitability and impairment of GABAergic function in the hippocampus of mice lacking trkB. Cereb Cortex 16(1):47-63. [PubMed: 15829735]  [MGI Ref ID J:174492]

Fagan AM; Zhang H; Landis S; Smeyne RJ; Silos-Santiago I; Barbacid M. 1996. TrkA, but not TrkC, receptors are essential for survival of sympathetic neurons in vivo. J Neurosci 16(19):6208-18. [PubMed: 8815902]  [MGI Ref ID J:35563]

Fan G; Copray S; Huang EJ; Jones K; Yan Q; Walro J; Jaenisch R; Kucera J. 2000. Formation of a full complement of cranial proprioceptors requires multiple neurotrophins. Dev Dyn 218(2):359-70. [PubMed: 10842362]  [MGI Ref ID J:62766]

Fritzsch B; Barbacid M; Silos-Santiago I. 1998. The combined effects of trkB and trkC mutations on the innervation of the inner ear. Int J Dev Neurosci 16(6):493-505. [PubMed: 9881298]  [MGI Ref ID J:52165]

Fritzsch B; Sarai PA; Barbacid M; Silos-Santiago I. 1997. Mice with a targeted disruption of the neurotrophin receptor trkB lose their gustatory ganglion cells early but do develop taste buds. Int J Dev Neurosci 15(4-5):563-76. [PubMed: 9263033]  [MGI Ref ID J:42663]

Fundin BT; Silos-Santiago I; Ernfors P; Fagan AM; Aldskogius H ; DeChiara TM ; Phillips HS ; Barbacid M ; Yancopoulos GD ; Rice FL. 1997. Differential dependency of cutaneous mechanoreceptors on neurotrophins, trk receptors, and P75 LNGFR. Dev Biol 190(1):94-116. [PubMed: 9331334]  [MGI Ref ID J:43425]

Garcia-Suarez O; Blanco-Gelaz MA; Lopez ML; Germana A; Cabo R; Diaz-Esnal B; Silos-Santiago I; Ciriaco E; Vega JA. 2002. Massive lymphocyte apoptosis in the thymus of functionally deficient TrkB mice. J Neuroimmunol 129(1-2):25-34. [PubMed: 12161017]  [MGI Ref ID J:102958]

Garcia-Suarez O; Perez-Pinera P; Laura R; Germana A; Esteban I; Cabo R; Silos-Santiago I; Cobo JL; Vega JA. 2009. TrkB is necessary for the normal development of the lung. Respir Physiol Neurobiol 167(3):281-91. [PubMed: 19523540]  [MGI Ref ID J:155643]

Gates MA; Tai CC; Macklis JD. 2000. Neocortical neurons lacking the protein-tyrosine kinase B receptor display abnormal differentiation and process elongation in vitro and in vivo. Neuroscience 98(3):437-47. [PubMed: 10869838]  [MGI Ref ID J:119169]

Gonzalez M; Ruggiero FP; Chang Q; Shi YJ; Rich MM; Kraner S; Balice-Gordon RJ. 1999. Disruption of Trkb-mediated signaling induces disassembly of postsynaptic receptor clusters at neuromuscular junctions [see comments] Neuron 24(3):567-83. [PubMed: 10595510]  [MGI Ref ID J:58563]

Gonzalez-Martinez T; Germana GP; Monjil DF; Silos-Santiago I; de Carlos F; Germana G; Cobo J; Vega JA. 2004. Absence of Meissner corpuscles in the digital pads of mice lacking functional TrkB. Brain Res 1002(1-2):120-8. [PubMed: 14988041]  [MGI Ref ID J:88786]

Holm PC; Rodriguez FJ; Kresse A; Canals JM; Silos-Santiago I; Arenas E. 2003. Crucial role of TrkB ligands in the survival and phenotypic differentiation of developing locus coeruleus noradrenergic neurons. Development 130(15):3535-45. [PubMed: 12810600]  [MGI Ref ID J:83661]

Ichikawa H; Matsuo S; Silos-Santiago I; Jacquin MF; Sugimoto T. 2001. Developmental dependency of Merkel endings on trks in the palate. Brain Res Mol Brain Res 88(1-2):171-5. [PubMed: 11295244]  [MGI Ref ID J:109307]

Ichikawa H; Matsuo S; Silos-Santiago I; Jacquin MF; Sugimoto T. 2004. The development of myelinated nociceptors is dependent upon trks in the trigeminal ganglion. Acta Histochem 106(5):337-43. [PubMed: 15530548]  [MGI Ref ID J:101950]

Ichikawa H; Matsuo S; Silos-Santiago I; Sugimoto T. 2000. Developmental dependency of Meissner corpuscles on trkB but not trkA or trkC Neuroreport 11(2):259-62. [PubMed: 10674466]  [MGI Ref ID J:60480]

Kraemer R; Baker PJ; Kent KC; Ye Y; Han JJ; Tejada R; Silane M; Upmacis R; Deeb R; Chen Y; Levine DM; Hempstead B. 2005. Decreased neurotrophin TrkB receptor expression reduces lesion size in the apolipoprotein E-null mutant mouse. Circulation 112(23):3644-53. [PubMed: 16330706]  [MGI Ref ID J:116881]

Kwapiszewska G; Chwalek K; Marsh LM; Wygrecka M; Wilhelm J; Best J; Egemnazarov B; Weisel FC; Osswald SL; Schermuly RT; Olschewski A; Seeger W; Weissmann N; Eickelberg O; Fink L. 2012. BDNF/TrkB signaling augments smooth muscle cell proliferation in pulmonary hypertension. Am J Pathol 181(6):2018-29. [PubMed: 23058367]  [MGI Ref ID J:190727]

Luikart BW; Nef S; Shipman T; Parada LF. 2003. In vivo role of truncated trkb receptors during sensory ganglion neurogenesis. Neuroscience 117(4):847-58. [PubMed: 12654337]  [MGI Ref ID J:104489]

Lush ME; Ma L; Parada LF. 2005. TrkB signaling regulates the developmental maturation of the somatosensory cortex. Int J Dev Neurosci 23(6):523-36. [PubMed: 16009525]  [MGI Ref ID J:103935]

Martinez A; Alcantara S; Borrell V; Del Rio JA; Blasi J; Otal R; Campos N; Boronat A; Barbacid M; Silos-Santiago I; Soriano E. 1998. TrkB and TrkC signaling are required for maturation and synaptogenesis of hippocampal connections. J Neurosci 18(18):7336-50. [PubMed: 9736654]  [MGI Ref ID J:49747]

Minichiello L; Casagranda F; Tatche RS; Stucky CL; Postigo A; Lewin GR; Davies AM; Klein R. 1998. Point mutation in trkB causes loss of NT4-dependent neurons without major effects on diverse BDNF responses. Neuron 21(2):335-45. [PubMed: 9728915]  [MGI Ref ID J:49472]

Minichiello L; Klein R. 1996. TrkB and TrkC neurotrophin receptors cooperate in promoting survival of hippocampal and cerebellar granule neurons. Genes Dev 10(22):2849-58. [PubMed: 8918886]  [MGI Ref ID J:36756]

Minichiello L; Korte M; Wolfer D; Kuhn R; Unsicker K; Cestari V; Rossi-Arnaud C; Lipp HP; Bonhoeffer T; Klein R. 1999. Essential role for TrkB receptors in hippocampus-mediated learning. Neuron 24(2):401-14. [PubMed: 10571233]  [MGI Ref ID J:58229]

Minichiello L; Piehl F; Vazquez E; Schimmang T; Hokfelt T; Represa J; Klein R. 1995. Differential effects of combined trk receptor mutations on dorsal root ganglion and inner ear sensory neurons. Development 121(12):4067-75. [PubMed: 8575307]  [MGI Ref ID J:30344]

Otal R; Martinez A; Soriano E. 2005. Lack of TrkB and TrkC signaling alters the synaptogenesis and maturation of mossy fiber terminals in the hippocampus. Cell Tissue Res 319(3):349-58. [PubMed: 15726425]  [MGI Ref ID J:105391]

Pinon LG; Minichiello L; Klein R; Davies AM. 1996. Timing of neuronal death in trkA, trkB and trkC mutant embryos reveals developmental changes in sensory neuron dependence on Trk signalling. Development 122(10):3255-61. [PubMed: 8898237]  [MGI Ref ID J:36236]

Polleux F; Whitford KL; Dijkhuizen PA; Vitalis T; Ghosh A. 2002. Control of cortical interneuron migration by neurotrophins and PI3-kinase signaling. Development 129(13):3147-60. [PubMed: 12070090]  [MGI Ref ID J:111369]

Pollock GS; Robichon R; Boyd KA; Kerkel KA; Kramer M; Lyles J; Ambalavanar R; Khan A; Kaplan DR; Williams RW; Frost DO. 2003. TrkB receptor signaling regulates developmental death dynamics, but not final number, of retinal ganglion cells. J Neurosci 23(31):10137-45. [PubMed: 14602830]  [MGI Ref ID J:88208]

Rice FL; Albers KM; Davis BM; Silos-Santiago I; Wilkinson GA; LeMaster AM; Ernfors P; Smeyne RJ; Aldskogius H; Phillips HS; Barbacid M; DeChiara TM; Yancopoulos GD; Dunne CE; Fundin BT. 1998. Differential dependency of unmyelinated and A delta epidermal and upper dermal innervation on neurotrophins, trk receptors, and p75LNGFR. Dev Biol 198(1):57-81. [PubMed: 9640332]  [MGI Ref ID J:107715]

Rose CR; Blum R; Pichler B; Lepier A; Kafitz KW; Konnerth A. 2003. Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells. Nature 426(6962):74-8. [PubMed: 14603320]  [MGI Ref ID J:180482]

Schimmang T; Alvarez-Bolado G; Minichiello L; Vazquez E; Giraldez F ; Klein R ; Represa J. 1997. Survival of inner ear sensory neurons in trk mutant mice. Mech Dev 64(1-2):77-85. [PubMed: 9232598]  [MGI Ref ID J:41896]

Schimmang T; Minichiello L; Vazquez E; San Jose I; Giraldez F; Klein R; Represa J. 1995. Developing inner ear sensory neurons require TrkB and TrkC receptors for innervation of their peripheral targets. Development 121(10):3381-91. [PubMed: 7588071]  [MGI Ref ID J:29245]

Schimmang T; Tan J; Muller M; Zimmermann U; Rohbock K; Kopschall I; Limberger A; Minichiello L; Knipper M. 2003. Lack of Bdnf and TrkB signalling in the postnatal cochlea leads to a spatial reshaping of innervation along the tonotopic axis and hearing loss. Development 130(19):4741-50. [PubMed: 12925599]  [MGI Ref ID J:84871]

Schober A; Minichiello L; Keller M; Huber K; Layer PG; Roig-Lopez JL ; Garcia-Arraras JE ; Klein R ; Unsicker K. 1997. Reduced acetylcholinesterase (AChE) activity in adrenal medulla and loss of sympathetic preganglionic neurons in TrkA-deficient, but not TrkB-deficient, mice. J Neurosci 17(3):891-903. [PubMed: 8994044]  [MGI Ref ID J:38394]

Schober A; Wolf N; Huber K; Hertel R; Krieglstein K; Minichiello L; Kahane N; Widenfalk J; Kalcheim C; Olson L; Klein R; Lewin GR; Unsicker K. 1998. TrkB and neurotrophin-4 are important for development and maintenance of sympathetic preganglionic neurons innervating the adrenal medulla. J Neurosci 18(18):7272-84. [PubMed: 9736648]  [MGI Ref ID J:120428]

Sciarretta C; Fritzsch B; Beisel K; Rocha-Sanchez SM; Buniello A; Horn JM; Minichiello L. 2010. PLCgamma-activated signalling is essential for TrkB mediated sensory neuron structural plasticity. BMC Dev Biol 10:103. [PubMed: 20932311]  [MGI Ref ID J:166067]

Sedy J; Szeder V; Walro JM; Ren ZG; Nanka O; Tessarollo L; Sieber-Blum M; Grim M; Kucera J. 2004. Pacinian corpuscle development involves multiple Trk signaling pathways. Dev Dyn 231(3):551-63. [PubMed: 15376326]  [MGI Ref ID J:93853]

Silos-Santiago I; Fagan AM; Garber M; Fritzsch B; Barbacid M. 1997. Severe sensory deficits but normal CNS development in newborn mice lacking TrkB and TrkC tyrosine protein kinase receptors. Eur J Neurosci 9(10):2045-56. [PubMed: 9421165]  [MGI Ref ID J:44795]

Spears N; Molinek MD; Robinson LL; Fulton N; Cameron H; Shimoda K; Telfer EE; Anderson RA; Price DJ. 2003. The role of neurotrophin receptors in female germ-cell survival in mouse and human. Development 130(22):5481-91. [PubMed: 14507777]  [MGI Ref ID J:85731]

Stankovski L; Alvarez C; Ouimet T; Vitalis T; El-Hachimi KH; Price D; Deneris E; Gaspar P; Cases O. 2007. Developmental cell death is enhanced in the cerebral cortex of mice lacking the brain vesicular monoamine transporter. J Neurosci 27(6):1315-24. [PubMed: 17287506]  [MGI Ref ID J:118338]

Stenqvist A; Agerman K; Marmigere F; Minichiello L; Ernfors P. 2005. Genetic evidence for selective neurotrophin 3 signalling through TrkC but not TrkB in vivo. EMBO Rep 6(10):973-8. [PubMed: 16142215]  [MGI Ref ID J:101705]

Suzuki K; Sato M; Morishima Y; Nakanishi S. 2005. Neuronal depolarization controls brain-derived neurotrophic factor-induced upregulation of NR2C NMDA receptor via calcineurin signaling. J Neurosci 25(41):9535-43. [PubMed: 16221864]  [MGI Ref ID J:101618]

Vitalis T; Cases O; Gillies K; Hanoun N; Hamon M; Seif I; Gaspar P; Kind P; Price DJ. 2002. Interactions between TrkB signaling and serotonin excess in the developing murine somatosensory cortex: a role in tangential and radial organization of thalamocortical axons. J Neurosci 22(12):4987-5000. [PubMed: 12077195]  [MGI Ref ID J:77515]

Wagner N; Wagner KD; Theres H; Englert C; Schedl A; Scholz H. 2005. Coronary vessel development requires activation of the TrkB neurotrophin receptor by the Wilms' tumor transcription factor Wt1. Genes Dev 19(21):2631-42. [PubMed: 16264195]  [MGI Ref ID J:102417]

Zhang C; Brandemihl A; Lau D; Lawton A; Oakley B. 1997. BDNF is required for the normal development of taste neurons in vivo. Neuroreport 8(4):1013-7. [PubMed: 9141083]  [MGI Ref ID J:40246]

de Carlos F; Cobo J; Germana G; Silos-Santiago I; Laura R; Haro JJ; Farinas I; Vega JA. 2006. Abnormal development of pacinian corpuscles in double trkB;trkC knockout mice. Neurosci Lett 410(3):157-61. [PubMed: 17101216]  [MGI Ref ID J:119011]

von Bohlen und Halbach O; Minichiello L; Unsicker K. 2005. Haploin-sufficiency for trkB and trkC receptors induces cell loss and accumulation of alpha-synuclein in the substantia nigra. FASEB J 19(12):1740-2. [PubMed: 16037097]  [MGI Ref ID J:102679]

von Bohlen und Halbach O; Minichiello L; Unsicker K. 2003. Haploinsufficiency in trkB and/or trkC neurotrophin receptors causes structural alterations in the aged hippocampus and amygdala. Eur J Neurosci 18(8):2319-25. [PubMed: 14622193]  [MGI Ref ID J:89676]

von Bohlen und Halbach O; Minichiello L; Unsicker K. 2008. TrkB but not trkC receptors are necessary for postnatal maintenance of hippocampal spines. Neurobiol Aging 29(8):1247-55. [PubMed: 17442456]  [MGI Ref ID J:140913]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain was generated on a 129 genetic background. It was subsequently bred to C57BL/6 mice. It is maintained either by mating heterozygous siblings or by mating heterozygous mice with normal wildtype siblings. It is currently being backcrossed to C57BL/6J. Expected coat color from breeding:Black
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3000.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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