Strain Name: |
STOCK Tg(CAG-EGFP)B5Nagy/J |
|---|---|
Stock Number: |
003115 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
STOCK Tg(ACTB-EGFP)B5Nagy/J (Changed: 08-MAY-08
) |
|
STOCK Tg(GFPU)5Nagy/J (Changed: 08-NOV-06
) | |
| Genes & Alleles | ACTB; GFP; Tg(CAG-EGFP)B5Nagy; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Andras Nagy, Mount Sinai Hospital Generation F?+22 (08-JAN-08) Appearance
white-bellied agouti
Related Genotype: Aw/?
black
Related Genotype: a/aStrain Description
This transgenic strain expresses an Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. Mice, and cells derived from them, are distinguished from wildtype on the basis of fluorescence. The transgene is expressed in all nucleated embryonic tissues. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. In newborn and adult mice, the entire organ system expresses EGFP. Though widespread, expression levels vary between different organs. This strain can be used as a source of fluorescently marked cells or tissues.Strain Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line B5/EGFP were sent to The Jackson Laboratory in 1998 and used to establish the colony on a mixed genetic background. Of note, some of these mice were used to generate a congenic colony (see Stock No. 003516).
Gene & Allele Details
| Allele Symbol | Tg(CAG-EGFP)B5Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion B5, Andras Nagy | ||
| Common Name(s) | B5/EGFP; Efgp; Tg (GFPU) 5Nagy; Tg(ACTB-EGFP)B5Nagy; Tg(GFPU)5Nagy; TgN(GFPU)5Nagy; | ||
| Mutation Made By | Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | The transgene is expressed in all nucleated embryonic tissues, Newborn and adult mice exhibit expression unbiquitously, though the level of expression varies in different organs. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | In transgenic newborn and adult mice, the entire organ system expresses GFP. While transgene expression is ubiquitous, expression levels vary between organs. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. | ||
| Molecular Note | The transgene carries the Green Fluorescent Protein driven by chicken beta actin promoter and CMV intermediate early enhancer. The construct was electroporated into R1 ES cells, for which the strain of origin is (129X1/SvJ x 129S1/Sv)F1-Kitl+.Random integration occurred on an unidentified autosome. The transgene is expressed in all nucleated embryonic tissues. [MGI Ref ID J:92926] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | This strain is currently maintained by breeding hemizygotes to wildtype siblings. Hemizygotes are reportedly distinguishable from homozygotes on the basis of fluorescence intensity using a Hungarian flashlight. Please refer to the primary reference for more information. Expected coat color from breeding:Black and White Bellied Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
003516 FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX? Mice Strains Carrying Fluorescent Proteins.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedHematological Research
Hemoglobin Defects
Research Tools
Cardiovascular Research
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesHadjantonakis AK; Gertsenstein M; Ikawa M; Okabe M; Nagy A. 1998. Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev 76(1-2):79-90. [PubMed: 9867352] [MGI Ref ID J:92926]
Price and Supply Information
| Strain Name: | STOCK Tg(CAG-EGFP)B5Nagy/J |
| Stock Number: | 003115 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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