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| This transgene, carrying the Enhanced Green Fluorescent Protein driven by chicken beta-actin promoter and CMV intermediate early enhancer, is integrated on the X chromosome. Transgene expression is seen as early as embryonic day 2.75, as morula stage embryos begin to compact. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tissues. | |||||||||||||||||||
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Description
Strain Information
Former Names STOCK Tg(ACTB-EGFP)D4Nagy/J (Changed: 08-MAY-08 ) STOCK Tg(GFPX)4Nagy/J (Changed: 08-NOV-06 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation [F1p]+F16 (11-APR-11)
Generation DefinitionsDonating Investigator Dr. Andras Nagy, Mount Sinai Hospital Appearance
mixed
Related Genotype:Description
This transgenic strain carries the Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. The transgene was found to have integrated on the X chromosome. Mice of this strain, and cells derived from them, can be distinguished from wildtype on the basis of fluorescence. Transgene expression is seen as early as ~embryonic day 2.75, as morula stage embryos begin to compact. Expression of EGFP is spatiotemporally widespread, though levels differ between different lineages (e.g., expression is slightly higher in the heart). As the transgene is located on the X chromosome, transgenic males will exclusively transmit the EGFP transgene to their female offspring, providing the basis for a non-invasive sexing assay based on green fluorescence. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tissues.Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line D4/XEGFP were sent to The Jackson Laboratory in 1998 and used to establish the colony on a mixed genetic background. This transgene has been found to have integrated on the X chromosome.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cardiovascular Research
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: GFP with actin, beta promoter/CMV intermediate early enhancer, X-linked
Reproductive Biology Research
GFP with actin, beta promoter/CMV intermediate early enhancer, X-linked
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-EGFP)D4Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion D4, Andras Nagy | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | D4/XEGFP; Tg(ACTB-EGFP)D4Nagy; Tg(CAG-EGFP)D4Nagy; Tg(CMV-GFP)1Jae; Tg(CMV-GFP)1Nagy; Tg(GFPX)4Nagy; X-GFP; XGFP; XGFP; Xp-GFP; | ||
| Mutation Made By | Dr. Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Integration is on the X Chromosome. The transgene is expressed in a spatiotemporally ubiquitous manner, with expression being slightly higher in heart than in other tissues. Transgene expression is seen as early as ~E2.75. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note |
Transgene expression is seen as fluorescence under exposure to a 488 nm light source. In transgenic mice the reporter may be detected as early as ~E2.75, as morula stage embryos begin to compact. Expression of GFP is spatiotemporally ubiquitous, though levels differ between different lineages (e.g., expression is slightly higher in the heart). No discernible phenotype, other than green fluorescence, has been observed in any mice carrying this transgene. | ||
| Molecular Note | The transgene contains the enhanced Green Fluorescent Protein driven by chicken beta-actin promoter and CMV intermediate early enhancer. Transgenic mice were generated by embryonic stem (ES) cell-mediated transgenesis. The construct was electroporated into R1 ES cells and random integration occurred on the X chromosome just distal of Timp and Syn1. [MGI Ref ID J:69995] | ||
| Gene Symbol and Name | Tg(CAG-EGFP)D4Nagy, transgene insertion D4, Andras Nagy | ||
| Chromosome | X | ||
| Gene Common Name(s) | D4/XEGFP; Tg(ACTB-EGFP)D4Nagy; Tg(CMV-GFP)1Jae; Tg(CMV-GFP)1Nagy; Tg(GFPX)4Nagy; X-GFP; XGFP; Xp-GFP; transgene insertion 1, Andras Nagy; transgene insertion 1, Rudolf Jaenisch; transgene insertion 4, Andras Nagy; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hadjantonakis AK; Gertsenstein M; Ikawa M; Okabe M; Nagy A. 1998. Non-invasive sexing of preimplantation stage mammalian embryos. Nat Genet 19(3):220-2. [PubMed: 9662390] [MGI Ref ID J:69995]
Additional References
Hadjantonakis AK; Cox LL; Tam PP; Nagy A. 2001. An X-linked GFP transgene reveals unexpected paternal X-chromosome activity in trophoblastic giant cells of the mouse placenta. Genesis 29(3):133-40. [PubMed: 11252054] [MGI Ref ID J:68706]
Tg(CAG-EGFP)D4Nagy relatedDiaz-Perez S; Ouyang Y; Perez V; Cisneros R; Regelson M; Marahrens Y. 2005. The element(s) at the nontranscribed Xist locus of the active X chromosome controls chromosomal replication timing in the mouse. Genetics 171(2):663-72. [PubMed: 15972460] [MGI Ref ID J:103500]
Drenckhahn JD; Schwarz QP; Gray S; Laskowski A; Kiriazis H; Ming Z; Harvey RP; Du XJ; Thorburn DR; Cox TC. 2008. Compensatory growth of healthy cardiac cells in the presence of diseased cells restores tissue homeostasis during heart development. Dev Cell 15(4):521-33. [PubMed: 18854137] [MGI Ref ID J:143285]
Gros J; Hu JK; Vinegoni C; Feruglio PF; Weissleder R; Tabin CJ. 2010. WNT5A/JNK and FGF/MAPK pathways regulate the cellular events shaping the vertebrate limb bud. Curr Biol 20(22):1993-2002. [PubMed: 21055947] [MGI Ref ID J:166897]
Hadjantonakis AK; Cox LL; Tam PP; Nagy A. 2001. An X-linked GFP transgene reveals unexpected paternal X-chromosome activity in trophoblastic giant cells of the mouse placenta. Genesis 29(3):133-40. [PubMed: 11252054] [MGI Ref ID J:68706]
Hanson JE; Madison DV. 2007. Presynaptic FMR1 genotype influences the degree of synaptic connectivity in a mosaic mouse model of fragile X syndrome. J Neurosci 27(15):4014-8. [PubMed: 17428978] [MGI Ref ID J:143837]
Iliff AJ; Renoux AJ; Krans A; Usdin K; Sutton MA; Todd PK. 2013. Impaired activity-dependent FMRP translation and enhanced mGluR-dependent LTD in Fragile X premutation mice. Hum Mol Genet 22(6):1180-92. [PubMed: 23250915] [MGI Ref ID J:193289]
Kalantry S; Magnuson T. 2006. The Polycomb group protein EED is dispensable for the initiation of random X-chromosome inactivation. PLoS Genet 2(5):e66. [PubMed: 16680199] [MGI Ref ID J:115851]
Kalantry S; Mills KC; Yee D; Otte AP; Panning B; Magnuson T. 2006. The Polycomb group protein Eed protects the inactive X-chromosome from differentiation-induced reactivation. Nat Cell Biol 8(2):195-202. [PubMed: 16415857] [MGI Ref ID J:129406]
Kashimada K; Svingen T; Feng CW; Pelosi E; Bagheri-Fam S; Harley VR; Schlessinger D; Bowles J; Koopman P. 2011. Antagonistic regulation of Cyp26b1 by transcription factors SOX9/SF1 and FOXL2 during gonadal development in mice. FASEB J 25(10):3561-9. [PubMed: 21757499] [MGI Ref ID J:178424]
Kobayashi A; Shawlot W; Kania A; Behringer RR. 2004. Requirement of Lim1 for female reproductive tract development. Development 131(3):539-49. [PubMed: 14695376] [MGI Ref ID J:133086]
Krentz AD; Murphy MW; Kim S; Cook MS; Capel B; Zhu R; Matin A; Sarver AL; Parker KL; Griswold MD; Looijenga LH; Bardwell VJ; Zarkower D. 2009. The DM domain protein DMRT1 is a dose-sensitive regulator of fetal germ cell proliferation and pluripotency. Proc Natl Acad Sci U S A 106(52):22323-8. [PubMed: 20007774] [MGI Ref ID J:156489]
Kwon GS; Viotti M; Hadjantonakis AK. 2008. The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages. Dev Cell 15(4):509-20. [PubMed: 18854136] [MGI Ref ID J:143286]
Mugford JW; Yee D; Magnuson T. 2012. Failure of extra-embryonic progenitor maintenance in the absence of dosage compensation. Development 139(12):2130-8. [PubMed: 22573614] [MGI Ref ID J:185555]
Namekawa SH; Payer B; Huynh KD; Jaenisch R; Lee JT. 2010. Two-step imprinted X inactivation: repeat versus genic silencing in the mouse. Mol Cell Biol 30(13):3187-205. [PubMed: 20404085] [MGI Ref ID J:162680]
Patel AB; Hays SA; Bureau I; Huber KM; Gibson JR. 2013. A target cell-specific role for presynaptic Fmr1 in regulating glutamate release onto neocortical fast-spiking inhibitory neurons. J Neurosci 33(6):2593-604. [PubMed: 23392687] [MGI Ref ID J:194272]
Polanco JC; Wilhelm D; Davidson TL; Knight D; Koopman P. 2010. Sox10 gain-of-function causes XX sex reversal in mice: implications for human 22q-linked disorders of sex development. Hum Mol Genet 19(3):506-16. [PubMed: 19933217] [MGI Ref ID J:155544]
Senner CE; Nesterova TB; Norton S; Dewchand H; Godwin J; Mak W; Brockdorff N. 2011. Disruption of a conserved region of Xist exon 1 impairs Xist RNA localisation and X-linked gene silencing during random and imprinted X chromosome inactivation. Development 138(8):1541-50. [PubMed: 21389056] [MGI Ref ID J:171502]
Seong IS; Woda JM; Song JJ; Lloret A; Abeyrathne PD; Woo CJ; Gregory G; Lee JM; Wheeler VC; Walz T; Kingston RE; Gusella JF; Conlon RA; Macdonald ME. 2009. Huntingtin facilitates polycomb repressive complex 2. Hum Mol Genet :. [PubMed: 19933700] [MGI Ref ID J:155886]
Simard AR; Rivest S. 2007. Neuroprotective effects of resident microglia following acute brain injury. J Comp Neurol 504(6):716-29. [PubMed: 17722035] [MGI Ref ID J:132520]
Smrt RD; Pfeiffer RL; Zhao X. 2011. Age-dependent expression of MeCP2 in a heterozygous mosaic mouse model. Hum Mol Genet 20(9):1834-43. [PubMed: 21330301] [MGI Ref ID J:170472]
Watson CM; Pelka GJ; Radziewic T; Shahbazian MD; Christodoulou J; Williamson SL; Tam PP. 2005. Reduced proportion of Purkinje cells expressing paternally derived mutant Mecp2308 allele in female mouse cerebellum is not due to a skewed primary pattern of X-chromosome inactivation. Hum Mol Genet 14(13):1851-61. [PubMed: 15888476] [MGI Ref ID J:105061]
Williams LH; Kalantry S; Starmer J; Magnuson T. 2011. Transcription precedes loss of Xist coating and depletion of H3K27me3 during X-chromosome reprogramming in the mouse inner cell mass. Development 138(10):2049-57. [PubMed: 21471155] [MGI Ref ID J:171449]
Yen WW; Williams M; Periasamy A; Conaway M; Burdsal C; Keller R; Lu X; Sutherland A. 2009. PTK7 is essential for polarized cell motility and convergent extension during mouse gastrulation. Development 136(12):2039-48. [PubMed: 19439496] [MGI Ref ID J:158149]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry The strain is maintained by mating hemizygous females with wildtype males. This transgene is X-linked: therefore, this mating scheme generates hemizygous males (Tg/Y) and hemizygous females (Tg/+) along with wildtype controls (+/+ females and +/Y males). Reproduction is very good. Although our mating scheme does not produce them, homozygous female transgenic mice are viable, and, reportedly, can be distinguished from hemizygous littermates on the basis of fluorescence intensity. Please refer to the primary reference for more information. Expected coat colors from breeding: multiple Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(CAG-EGFP)D4Nagy
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(CAG-EGFP)D4Nagy x Noncarrier Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(CAG-EGFP)D4Nagy
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(CAG-EGFP)D4Nagy x Noncarrier Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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