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Description
Strain Information
Former Names STOCK Tg(ACTB-EGFP)D4Nagy/J (Changed: 08-MAY-08 ) STOCK Tg(GFPX)4Nagy/J (Changed: 08-NOV-06 ) Type Mutant Stock; Transgenic; Species laboratory mouse Generation F?+7p+F1p (06-NOV-07) Donating Investigator Andras Nagy, Mount Sinai Hospital Appearance
mixed
Related Genotype:Description
This transgenic strain carries the Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. The transgene was found to have integrated on the X chromosome. Mice of this strain, and cells derived from them, can be distinguished from wildtype on the basis of fluorescence. Transgene expression is seen as early as ~embryonic day 2.75, as morula stage embryos begin to compact. Expression of EGFP is spatiotemporally widespread, though levels differ between different lineages (e.g., expression is slightly higher in the heart). As the transgene is located on the X chromosome, transgenic males will exclusively transmit the EGFP transgene to their female offspring, providing the basis for a non-invasive sexing assay based on green fluorescence. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tissues.Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line D4/XEGFP were sent to The Jackson Laboratory in 1998 and used to establish the colony on a mixed genetic background. This transgene has been found to have integrated on the X chromosome.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (157 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cardiovascular Research
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: GFP with actin, beta promoter/CMV intermediate early enhancer, X-linked)
Reproductive Biology Research (GFP with actin, beta promoter/CMV intermediate early enhancer, X-linked)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-EGFP)D4Nagy | ||
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| Allele Name | transgene insertion D4, Andras Nagy | ||
| Common Name(s) | D4/XEGFP; Tg(ACTB-EGFP)D4Nagy; Tg(CAG-EGFP)D4Nagy; Tg(CMV-GFP)1Jae; Tg(CMV-GFP)1Nagy; Tg(GFPX)4Nagy; X-GFP; XGFP; Xp-GFP; | ||
| Mutation Made By | Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Integration is on the X Chromosome. The transgene is expressed in a spatiotemporally ubiquitous manner, with expression being slightly higher in heart than in other tissues. Transgene expression is seen as early as ~E2.75. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note |
Transgene expression is seen as fluorescence under exposure to a 488 nm light source. In transgenic mice the reporter may be detected as early as ~E2.75, as morula stage embryos begin to compact. Expression of GFP is spatiotemporally ubiquitous, though levels differ between different lineages (e.g., expression is slightly higher in the heart). No discernible phenotype, other than green fluorescence, has been observed in any mice carrying this transgene. | ||
| Molecular Note | The transgene contains the enhanced Green Fluorescent Protein driven by chicken beta-actin promoter and CMV intermediate early enhancer. Transgenic mice were generated by embryonic stem (ES) cell-mediated transgenesis. The construct was electroporated into R1 ES cells and random integration occurred on the X chromosome just distal of Timp and Syn1. [MGI Ref ID J:69995] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Hadjantonakis AK; Gertsenstein M; Ikawa M; Okabe M; Nagy A. 1998. Non-invasive sexing of preimplantation stage mammalian embryos. Nat Genet 19(3):220-2. [PubMed: 9662390] [MGI Ref ID J:69995]
Additional References
Hadjantonakis AK; Cox LL; Tam PP; Nagy A. 2001. An X-linked GFP transgene reveals unexpected paternal X-chromosome activity in trophoblastic giant cells of the mouse placenta. Genesis 29(3):133-40. [PubMed: 11252054] [MGI Ref ID J:68706]
Tg(CAG-EGFP)D4Nagy related
Diaz-Perez S; Ouyang Y; Perez V; Cisneros R; Regelson M; Marahrens Y. 2005. The element(s) at the nontranscribed Xist locus of the active X chromosome controls chromosomal replication timing in the mouse. Genetics 171(2):663-72. [PubMed: 15972460] [MGI Ref ID J:103500]Hadjantonakis AK; Cox LL; Tam PP; Nagy A. 2001. An X-linked GFP transgene reveals unexpected paternal X-chromosome activity in trophoblastic giant cells of the mouse placenta. Genesis 29(3):133-40. [PubMed: 11252054] [MGI Ref ID J:68706]
Kalantry S; Magnuson T. 2006. The Polycomb group protein EED is dispensable for the initiation of random X-chromosome inactivation. PLoS Genet 2(5):e66. [PubMed: 16680199] [MGI Ref ID J:115851]
Kalantry S; Mills KC; Yee D; Otte AP; Panning B; Magnuson T. 2006. The Polycomb group protein Eed protects the inactive X-chromosome from differentiation-induced reactivation. Nat Cell Biol 8(2):195-202. [PubMed: 16415857] [MGI Ref ID J:129406]
Kobayashi A; Shawlot W; Kania A; Behringer RR. 2004. Requirement of Lim1 for female reproductive tract development. Development 131(3):539-49. [PubMed: 14695376] [MGI Ref ID J:133086]
Simard AR; Rivest S. 2007. Neuroprotective effects of resident microglia following acute brain injury. J Comp Neurol 504(6):716-29. [PubMed: 17722035] [MGI Ref ID J:132520]
Watson CM; Pelka GJ; Radziewic T; Shahbazian MD; Christodoulou J; Williamson SL; Tam PP. 2005. Reduced proportion of Purkinje cells expressing paternally derived mutant Mecp2308 allele in female mouse cerebellum is not due to a skewed primary pattern of X-chromosome inactivation. Hum Mol Genet 14(13):1851-61. [PubMed: 15888476] [MGI Ref ID J:105061]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(CAG-EGFP)D4Nagy *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(CAG-EGFP)D4Nagy x Noncarrier
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(CAG-EGFP)D4Nagy *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(CAG-EGFP)D4Nagy x Noncarrier
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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