Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129S-Smsttm1Ute    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation pN2p Generation Definitions   Donating Investigator Dr. Ute Hochgeschwender,   Duke Institute for Brain Science

Description
Mutant mice are healthy, fertile and of normal body size despite having three times the plasma growth hormone levels than wildtype littermates. Somatostatin is virtually absent in the adult cerebellum but is expressed during development. There is impaired motor learning and motor coordination as demonstrated in accelerating rotorod testing.

Development
An insertion of selection cassette into the preprosomatostatin locus, thereby deleting 30 basepairs of the 3' end of exon 1 and 39 basepairs of the 5' end of intron 1.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying   Sst^tm1Ute allele

008117

B6.129S4(129S6)-Ssttm1Ute/J

View Strains carrying   Sst^tm1Ute     (1 strain)

Strains carrying other alleles of Sst

010708	B6(Cg)-Ssttm1(cre/ERT2)Zjh/J
016868	B6.Cg-Ssttm1.2(tTA2)Hze/J
013044	STOCK Ssttm2.1(cre)Zjh/J

View Strains carrying other alleles of Sst     (3 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Sst^tm1Ute/Sst^tm1Ute

        129-Sst^tm1Ute/J

• behavior/neurological phenotype
• *normal* behavior/neurological phenotype
  • no overt behavioral abnormalities, including reflexes, anxiety related behaviors, sensorimotor responses, fear conditioning and exploratory activity   (MGI Ref ID J:70426)
• • abnormal motor learning
    • motor learning performance is impaired on the accelerating rotarod test; mutant mice perform similarly to controls in the first two training sessions, but do not improve from trial 2 to 3 as do control mice   (MGI Ref ID J:70426)
    • mutant mice perform similar to controls in coordination, strength and balance tests such as vertical pole and hanging wire test   (MGI Ref ID J:70426)
• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype
  • T4 plasma levels are similar to controls   (MGI Ref ID J:70426)
  • plasma glucose levels are similar to controls   (MGI Ref ID J:70426)
• • increased circulating corticosterone level
    • plasma corticosterone levels are significantly elevated compared to controls   (MGI Ref ID J:70426)
  • increased circulating growth hormone level
    • plasma growth hormone levels are 3 times greater than controls but body weight is normal   (MGI Ref ID J:70426)
• nervous system phenotype
• *normal* nervous system phenotype
  • no gross abnormalities of brain morphology, including the characteristic layering of cells in the cerebellar cortex   (MGI Ref ID J:70426)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Sst^tm1Ute/Sst^tm1Ute

        involves: 129S4/SvJae * C57BL/6

• digestive/alimentary phenotype
• abnormal gastric gland morphology   (MGI Ref ID J:97527)
• • abnormal gastric parietal cell morphology
    • increased numbers of parietal cells   (MGI Ref ID J:97527)
• abnormal stomach fundus morphology
  • thickened fundus   (MGI Ref ID J:97527)
• enlarged stomach
  • stomachs heavier than in controls   (MGI Ref ID J:97527)
• hyperchlorhydria
  • hyperacidic   (MGI Ref ID J:97527)

Sst^tm1Ute/Sst^tm1Ute

        involves: 129S4/SvJae * C57BL/6J

• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype
  • mice exhibit normal insulin sensitivity   (MGI Ref ID J:179095)
• • decreased circulating prolactin level
    • in female and male mice   (MGI Ref ID J:179095)
  • increased circulating ghrelin level
    • female and male mice exhibit increased plasma total ghrelin levels compared with wild-type mice   (MGI Ref ID J:179095)
    • however, acylated ghrelin levels are normal   (MGI Ref ID J:179095)
  • increased circulating insulin level
    • in male, but not female, mice   (MGI Ref ID J:179095)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Sst^tm1Ute related

Cancer Research
Genes Regulating Growth and Proliferation

Endocrine Deficiency Research
Hypothalamus/Pituitary Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Ssttm1Ute
Allele Name		targeted mutation 1, Ute Hochgeschwender
Allele Type		Targeted (knock-out)
Common Name(s)		Srif-; Sst KO; Sst-;
Mutation Made By		Dr. Ute Hochgeschwender,   Duke Institute for Brain Science
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Sst, somatostatin
Chromosome		16
Gene Common Name(s)		SMST; SOM; SRIF; SS-14; SS-28; Smst; preprosomatostatin;
Molecular Note		An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated thelack of protein from homozygous mutants. [MGI Ref ID J:70426]

Genotyping

Genotyping Information

Genotyping Protocols

Sst^tm1Ute, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zeyda T; Diehl N; Paylor R; Brennan MB; Hochgeschwender U. 2001. Impairment in motor learning of somatostatin null mutant mice. Brain Res 906(1-2):107-14. [PubMed: 11430867]  [MGI Ref ID J:70426]

Additional References

Sst^tm1Ute related

Cordoba-Chacon J; Gahete MD; Castano JP; Kineman RD; Luque RM. 2011. Somatostatin and its receptors contribute in a tissue-specific manner to the sex-dependent metabolic (fed/fasting) control of growth hormone axis in mice. Am J Physiol Endocrinol Metab 300(1):E46-54. [PubMed: 20943754]  [MGI Ref ID J:172331]

Cordoba-Chacon J; Gahete MD; Pozo-Salas AI; Martinez-Fuentes AJ; de Lecea L; Gracia-Navarro F; Kineman RD; Castano JP; Luque RM. 2011. Cortistatin is not a somatostatin analogue but stimulates prolactin release and inhibits GH and ACTH in a gender-dependent fashion: potential role of ghrelin. Endocrinology 152(12):4800-12. [PubMed: 21971153]  [MGI Ref ID J:179095]

Laz EV; Holloway MG; Chen CS; Waxman DJ. 2007. Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liver. Endocrinology 148(7):3327-37. [PubMed: 17412818]  [MGI Ref ID J:129617]

Luque RM; Gahete MD; Hochgeschwender U; Kineman RD. 2006. Evidence that endogenous SST inhibits ACTH and ghrelin expression by independent pathways. Am J Physiol Endocrinol Metab 291(2):E395-403. [PubMed: 16825606]  [MGI Ref ID J:127795]

Luque RM; Soares BS; Peng XD; Krishnan S; Cordoba-Chacon J; Frohman LA; Kineman RD. 2009. Use of the metallothionein promoter-human growth hormone-releasing hormone (GHRH) mouse to identify regulatory pathways that suppress pituitary somatotrope hyperplasia and adenoma formation due to GHRH-receptor hyperactivation. Endocrinology 150(7):3177-85. [PubMed: 19342460]  [MGI Ref ID J:158164]

Meyer RD; Laz EV; Su T; Waxman DJ. 2009. Male-specific hepatic Bcl6: growth hormone-induced block of transcription elongation in females and binding to target genes inversely coordinated with STAT5. Mol Endocrinol 23(11):1914-26. [PubMed: 19797429]  [MGI Ref ID J:153887]

Saito T; Iwata N; Tsubuki S; Takaki Y; Takano J; Huang SM; Suemoto T; Higuchi M; Saido TC. 2005. Somatostatin regulates brain amyloid beta peptide Abeta(42) through modulation of proteolytic degradation. Nat Med 11(4):434-9. [PubMed: 15778722]  [MGI Ref ID J:97539]

Videau C; Hochgeschwender U; Kreienkamp HJ; Brennan MB; Viollet C; Richter D; Epelbaum J. 2003. Characterisation of [125I]-Tyr0DTrp8-somatostatin binding in sst1- to sst4- and SRIF-gene-invalidated mouse brain. Naunyn Schmiedebergs Arch Pharmacol 367(6):562-71. [PubMed: 12759718]  [MGI Ref ID J:95635]

Zavros Y; Eaton KA; Kang W; Rathinavelu S; Katukuri V; Kao JY; Samuelson LC; Merchant JL. 2005. Chronic gastritis in the hypochlorhydric gastrin-deficient mouse progresses to adenocarcinoma. Oncogene 24(14):2354-66. [PubMed: 15735748]  [MGI Ref ID J:97527]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	The originating investigator maintains the strain by mating heterozygous siblings. Litter size averages 6 pups. Homozygous animals are viable and fertile. Expected coat color from breeding:White Bellied Agouti

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.