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Former Names 129S-Smsttm1Ute (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation pN2p Donating Investigator Ute Hochgeschwender, Oklahoma Medical Research Foundation Description
Mutant mice are healthy, fertile and of normal body size despite having three times the plasma growth hormone levels than wildtype littermates. Somatostatin is virtually absent in the adult cerebellum but is expressed during development. There is impaired motor learning and motor coordination as demonstrated in accelerating rotorod testing.Development
An insertion of selection cassette into the preprosomatostatin locus, thereby deleting 30 basepairs of the 3' end of exon 1 and 39 basepairs of the 5' end of intron 1.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Strains carrying Ssttm1Ute allele
008117 B6.129S4(129S6)-Ssttm1Ute/J View Strains carrying Ssttm1Ute (1 strain)
New 129 Nomenclature Bulletin
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ssttm1Ute/Ssttm1Ute
involves: 129S4/SvJae * C57BL/6
- digestive/alimentary phenotype
- abnormal gastric gland (MGI Ref ID J:97527)
- abnormal gastric parietal cell morphology (MGI Ref ID J:97527)
- increased numbers of parietal cells
- enlarged stomach (MGI Ref ID J:97527)
- thickened fundus but no tumors
- stomachs heavier than in controls
- hyperchlorhydria (MGI Ref ID J:97527)
- hyperacidic
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Ssttm1Ute related
Cancer Research
Genes Regulating Growth and Proliferation
Endocrine Deficiency Research
Hypothalamus/Pituitary Defects
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Inflammation
Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects
| Allele Symbol | Ssttm1Ute | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ute Hochgeschwender | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Srif-; Sst KO; Sst-; | ||
| Mutation Made By | Ute Hochgeschwender, Oklahoma Medical Research Foundation | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Sst, somatostatin | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | SMST; SOM; SRIF; SS-14; SS-28; Smst; preprosomatostatin; | ||
| Molecular Note | An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated thelack of protein from homozygous mutants. [MGI Ref ID J:70426] | ||
Genotyping Protocols
Ssttm1Ute, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Zeyda T; Diehl N; Paylor R; Brennan MB; Hochgeschwender U. 2001. Impairment in motor learning of somatostatin null mutant mice. Brain Res 906(1-2):107-14. [PubMed: 11430867] [MGI Ref ID J:70426]
Ssttm1Ute relatedLaz EV; Holloway MG; Chen CS; Waxman DJ. 2007. Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liver. Endocrinology 148(7):3327-37. [PubMed: 17412818] [MGI Ref ID J:129617]
Luque RM; Gahete MD; Hochgeschwender U; Kineman RD. 2006. Evidence that endogenous SST inhibits ACTH and ghrelin expression by independent pathways. Am J Physiol Endocrinol Metab 291(2):E395-403. [PubMed: 16825606] [MGI Ref ID J:127795]
Saito T; Iwata N; Tsubuki S; Takaki Y; Takano J; Huang SM; Suemoto T; Higuchi M; Saido TC. 2005. Somatostatin regulates brain amyloid beta peptide Abeta(42) through modulation of proteolytic degradation. Nat Med 11(4):434-9. [PubMed: 15778722] [MGI Ref ID J:97539]
Videau C; Hochgeschwender U; Kreienkamp HJ; Brennan MB; Viollet C; Richter D; Epelbaum J. 2003. Characterisation of [125I]-Tyr0DTrp8-somatostatin binding in sst1- to sst4- and SRIF-gene-invalidated mouse brain. Naunyn Schmiedebergs Arch Pharmacol 367(6):562-71. [PubMed: 12759718] [MGI Ref ID J:95635]
Zavros Y; Eaton KA; Kang W; Rathinavelu S; Katukuri V; Kao JY; Samuelson LC; Merchant JL. 2005. Chronic gastritis in the hypochlorhydric gastrin-deficient mouse progresses to adenocarcinoma. Oncogene 24(14):2354-66. [PubMed: 15735748] [MGI Ref ID J:97527]
Colony Maintenance
Breeding & Husbandry The originating investigator maintains the strain by mating heterozygous siblings. Litter size averages 6 pups. Homozygous animals are viable and fertile. Expected coat color from breeding:White Bellied Agouti
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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