Description

Strain Information

Former Names B6;129X1-Grprtm1Jfb/J    (Changed: 30-NOV-05 ) Type Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Lori Hampton,   NIDCD-NIH

Appearance
black
Related Genotype: a/a

Description
Mice homozygous (females) or hemizygous (males) for the X-linked Grpr^tm1Jfb targeted mutation are viable with no gross phenotypic abnormalities observed. There is a deficiency in bombesin-induced mediation of satiety as measured by glucose intake. A modest increase in body weight is observed in older animals. At 18-20 months of age, hemizygous males (N = 8) are 44.5g ± 5.1 (mean ± std dev) and wildtype littermates (N = 8) are 38.3g ± 5.1 (L. Hampton, unpublished observation). We have recently observed head tilts and bobbing in the hemizygous males in our production colony. The donating investigator of this strain has also observed this phenotype and considers it a mutation not related to the gene. We are working to remove this phenotype, but we will continue to ship from the colony while we finish this process.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 8 generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Grpr^tm1Jfb/Grpr^tm1Jfb

        involves: 129X1/SvJ * C57BL/6J

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:46897)
  • female homozygotes are viable, fertile and developmentally normal with no pathological or histological abnormalities in all major organ systems up to 10 months of age

Grpr^tm1Jfb/Y

        involves: 129X1/SvJ * C57BL/6J

• behavior/neurological phenotype
• abnormal food intake (MGI Ref ID J:46897)
  • male hemizygotes are viable, fertile and developmentally normal with no pathological or histological abnormalities in all major organ systems up to 10 months of age
  • however, male hemizygotes lack a bombesin (BN)-induced satiety response: BN produces a significant suppression of glucose intake in wild-type mice at doses of 3.2, 10, and 32 nmol/kg but fails to suppress glucose intake at any dose with no apparent weight gain in mutant males

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Grpr^tm1Jfb related

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Diabetes and Obesity Research
Obesity Without Diabetes (moderate, adult onset)

Endocrine Deficiency Research
Gastrointestinal Defects
Pancreas Defects

Neurobiology Research
Behavioral and Learning Defects
Metabolic Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Grprtm1Jfb
Allele Name		targeted mutation 1, James F Battey
Allele Type		Targeted (knock-out)
Common Name(s)		GRP-R-;
Mutation Made By		Lori Hampton,   NIDCD-NIH
Strain of Origin		129X1/SvJ
ES Cell Line Name		RW-4
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Grpr, gastrin releasing peptide receptor
Chromosome		X
Gene Common Name(s)		GRP-R; bombesin receptor;
Molecular Note		A neomycin resistance cassette disrupted exon 1 of the gene. [MGI Ref ID J:46897]

Genotyping

Genotyping Information

Genotyping Protocols

Grpr^tm1Jfb, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Hampton LL; Ladenheim EE; Akeson M; Way JM; Weber HC; Sutliff VE ; Jensen RT ; Wine LJ ; Arnheiter H ; Battey JF. 1998. Loss of bombesin-induced feeding suppression in gastrin-releasing peptide receptor-deficient mice. Proc Natl Acad Sci U S A 95(6):3188-92. [PubMed: 9501238]  [MGI Ref ID J:46897]

Additional References

Hampton LL; Wright CG; Alagramam KN; Battey JF; Noben-Trauth K. 2003. A new spontaneous mutation in the mouse Ames waltzer gene, Pcdh15. Hear Res 180(1-2):67-75. [PubMed: 12782354]  [MGI Ref ID J:84779]

Ladenheim EE; Hampton LL; Whitney AC; White WO; Battey JF; Moran TH. 2002. Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. J Endocrinol 174(2):273-81. [PubMed: 12176666]  [MGI Ref ID J:78753]

Grpr^tm1Jfb related

Carroll RE; Matkowskyj K; Saunthararajah Y; Sekosan M; Battey JF; Benya RV. 2002. Contribution of gastrin-releasing peptide and its receptor to villus development in the murine and human gastrointestinal tract. Mech Dev 113(2):121-30. [PubMed: 11960700]  [MGI Ref ID J:76548]

Carroll RE; Matkowskyj KA; Tretiakova MS; Battey JF; Benya RV. 2000. Gastrin-releasing peptide is a mitogen and a morphogen in murine colon cancer Cell Growth Differ 11(7):385-93. [PubMed: 10939592]  [MGI Ref ID J:63661]

Ladenheim EE; Hampton LL; Whitney AC; White WO; Battey JF; Moran TH. 2002. Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. J Endocrinol 174(2):273-81. [PubMed: 12176666]  [MGI Ref ID J:78753]

Shumyatsky GP; Tsvetkov E; Malleret G; Vronskaya S; Hatton M; Hampton L; Battey JF; Dulac C; Kandel ER; Bolshakov VY. 2002. Identification of a signaling network in lateral nucleus of amygdala important for inhibiting memory specifically related to learned fear. Cell 111(6):905-18. [PubMed: 12526815]  [MGI Ref ID J:80849]

Sun YG; Chen ZF. 2007. A gastrin-releasing peptide receptor mediates the itch sensation in the spinal cord. Nature 448(7154):700-3. [PubMed: 17653196]  [MGI Ref ID J:123810]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred by crossing heterozygous or homozygous females and hemizygous males. This is a targeted mutation of an X-linked gene. Expected coat color from breeding: Black.
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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