Strain Name:

B6.129X1-Grprtm1Jfb/J

Stock Number:

003126

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129X1-Grprtm1Jfb/J    (Changed: 30-NOV-05 )
Type Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Lori Hampton,   NIDCD-NIH

Appearance
black
Related Genotype: a/a

Description
Mice homozygous (females) or hemizygous (males) for the X-linked Grprtm1Jfb targeted mutation are viable with no gross phenotypic abnormalities observed. There is a deficiency in bombesin-induced mediation of satiety as measured by glucose intake. A modest increase in body weight is observed in older animals. At 18-20 months of age, hemizygous males (N = 8) are 44.5g ± 5.1 (mean ± std dev) and wildtype littermates (N = 8) are 38.3g ± 5.1 (L. Hampton, unpublished observation). We have recently observed head tilts and bobbing in the hemizygous males in our production colony. The donating investigator of this strain has also observed this phenotype and considers it a mutation not related to the gene. We are working to remove this phenotype, but we will continue to ship from the colony while we finish this process.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 8 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Grprtm1Jfb/Grprtm1Jfb

        involves: 129X1/SvJ * C57BL/6J
  • normal phenotype
  • no abnormal phenotype detected
    • female homozygotes are viable, fertile and developmentally normal with no pathological or histological abnormalities in all major organ systems up to 10 months of age   (MGI Ref ID J:46897)

Grprtm1Jfb/Y

        involves: 129X1/SvJ * C57BL/6J
  • behavior/neurological phenotype
  • abnormal food intake
    • male hemizygotes are viable, fertile and developmentally normal with no pathological or histological abnormalities in all major organ systems up to 10 months of age   (MGI Ref ID J:46897)
    • however, male hemizygotes lack a bombesin (BN)-induced satiety response: BN produces a significant suppression of glucose intake in wild-type mice at doses of 3.2, 10, and 32 nmol/kg but fails to suppress glucose intake at any dose with no apparent weight gain in mutant males   (MGI Ref ID J:46897)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Grprtm1Jfb related

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Diabetes and Obesity Research
Obesity Without Diabetes
      moderate, adult onset

Endocrine Deficiency Research
Gastrointestinal Defects
Pancreas Defects

Neurobiology Research
Behavioral and Learning Defects
Metabolic Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Grprtm1Jfb
Allele Name targeted mutation 1, James F Battey
Allele Type Targeted (knock-out)
Common Name(s) GRP-R-; GRPR-;
Mutation Made ByDr. Lori Hampton,   NIDCD-NIH
Strain of Origin129X1/SvJ
ES Cell Line NameRW-4
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Grpr, gastrin releasing peptide receptor
Chromosome X
Gene Common Name(s) BB2; GRP-R; bombesin receptor;
Molecular Note A neomycin resistance cassette disrupted exon 1 of the gene. [MGI Ref ID J:46897]

Genotyping

Genotyping Information

Genotyping Protocols

Grprtm1Jfb,

Separated MCA


Grprtm1Jfb, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hampton LL; Ladenheim EE; Akeson M; Way JM; Weber HC; Sutliff VE ; Jensen RT ; Wine LJ ; Arnheiter H ; Battey JF. 1998. Loss of bombesin-induced feeding suppression in gastrin-releasing peptide receptor-deficient mice. Proc Natl Acad Sci U S A 95(6):3188-92. [PubMed: 9501238]  [MGI Ref ID J:46897]

Additional References

Hampton LL; Wright CG; Alagramam KN; Battey JF; Noben-Trauth K. 2003. A new spontaneous mutation in the mouse Ames waltzer gene, Pcdh15. Hear Res 180(1-2):67-75. [PubMed: 12782354]  [MGI Ref ID J:84779]

Ladenheim EE; Hampton LL; Whitney AC; White WO; Battey JF; Moran TH. 2002. Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. J Endocrinol 174(2):273-81. [PubMed: 12176666]  [MGI Ref ID J:78753]

Grprtm1Jfb related

Balci F; Papachristos EB; Gallistel CR; Brunner D; Gibson J; Shumyatsky GP. 2008. Interval timing in genetically modified mice: a simple paradigm. Genes Brain Behav 7(3):373-84. [PubMed: 17696995]  [MGI Ref ID J:147479]

Carroll RE; Matkowskyj K; Saunthararajah Y; Sekosan M; Battey JF; Benya RV. 2002. Contribution of gastrin-releasing peptide and its receptor to villus development in the murine and human gastrointestinal tract. Mech Dev 113(2):121-30. [PubMed: 11960700]  [MGI Ref ID J:76548]

Carroll RE; Matkowskyj KA; Tretiakova MS; Battey JF; Benya RV. 2000. Gastrin-releasing peptide is a mitogen and a morphogen in murine colon cancer Cell Growth Differ 11(7):385-93. [PubMed: 10939592]  [MGI Ref ID J:63661]

Chaperon F; Fendt M; Kelly PH; Lingenhoehl K; Mosbacher J; Olpe HR; Schmid P; Sturchler C; McAllister KH; van der Putten PH; Gee CE. 2012. Gastrin-releasing peptide signaling plays a limited and subtle role in amygdala physiology and aversive memory. PLoS One 7(4):e34963. [PubMed: 22509372]  [MGI Ref ID J:187094]

Ladenheim EE; Hampton LL; Whitney AC; White WO; Battey JF; Moran TH. 2002. Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. J Endocrinol 174(2):273-81. [PubMed: 12176666]  [MGI Ref ID J:78753]

Lagerstrom MC; Rogoz K; Abrahamsen B; Persson E; Reinius B; Nordenankar K; Olund C; Smith C; Mendez JA; Chen ZF; Wood JN; Wallen-Mackenzie A; Kullander K. 2010. VGLUT2-dependent sensory neurons in the TRPV1 population regulate pain and itch. Neuron 68(3):529-42. [PubMed: 21040852]  [MGI Ref ID J:167752]

Liu XY; Liu ZC; Sun YG; Ross M; Kim S; Tsai FF; Li QF; Jeffry J; Kim JY; Loh HH; Chen ZF. 2011. Unidirectional Cross-Activation of GRPR by MOR1D Uncouples Itch and Analgesia Induced by Opioids. Cell 147(2):447-58. [PubMed: 22000021]  [MGI Ref ID J:177501]

Martel G; Hevi C; Wong A; Zushida K; Uchida S; Shumyatsky GP. 2012. Murine GRPR and stathmin control in opposite directions both cued fear extinction and neural activities of the amygdala and prefrontal cortex. PLoS One 7(2):e30942. [PubMed: 22312434]  [MGI Ref ID J:185238]

Shumyatsky GP; Tsvetkov E; Malleret G; Vronskaya S; Hatton M; Hampton L; Battey JF; Dulac C; Kandel ER; Bolshakov VY. 2002. Identification of a signaling network in lateral nucleus of amygdala important for inhibiting memory specifically related to learned fear. Cell 111(6):905-18. [PubMed: 12526815]  [MGI Ref ID J:80849]

Sun YG; Chen ZF. 2007. A gastrin-releasing peptide receptor mediates the itch sensation in the spinal cord. Nature 448(7154):700-3. [PubMed: 17653196]  [MGI Ref ID J:123810]

Sun YG; Zhao ZQ; Meng XL; Yin J; Liu XY; Chen ZF. 2009. Cellular basis of itch sensation. Science 325(5947):1531-4. [PubMed: 19661382]  [MGI Ref ID J:152477]

Zhao ZQ; Huo FQ; Jeffry J; Hampton L; Demehri S; Kim S; Liu XY; Barry DM; Wan L; Liu ZC; Li H; Turkoz A; Ma K; Cornelius LA; Kopan R; Battey JF Jr; Zhong J; Chen ZF. 2013. Chronic itch development in sensory neurons requires BRAF signaling pathways. J Clin Invest 123(11):4769-80. [PubMed: 24216512]  [MGI Ref ID J:205122]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred by crossing heterozygous or homozygous females and hemizygous males. This is a targeted mutation of an X-linked gene. Expected coat color from breeding: Black.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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