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Former Names C57BL/6J-Epha4rb-2J/J (Changed: 22-JUN-09 ) C57BL/6J-rb2J/+ (Changed: 15-DEC-04 ) Type Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse H2 Haplotype b Generation F200+F23+N1p Appearance
black, hopping gait
Related Genotype: a/a Epha4rb-2J/Epha4rb-2J
black, unaffected
Related Genotype: a/a Epha4rb-2J/+ or a/a +/?Important Note
The C57BL/6J background strain is homozygous for the age related hearing loss mutation Cdh23ahl, which on this background causes progressive hearing loss with onset after 10 months of age.Description
Mice homozygous for the Epha4rb-2J allele can be visibly identified by 2.5 - 3 weeks of age by a constant hopping gait of the hind limbs; the forelimbs may show a hopping gait or move normally. When picked up by the tail mutants clasp their hindlimbs and show reduced ability to hold on to an edge; forelimbs show normal strength. More severely affected homozygous mutant mice also lean frequently to either side, a phenotype not reported in mice homozygous for the rb allele of Epha4. Homozygous females may deliver 2-3 pups per litter but often exhibit poor nurturing.Development
The rb2J allele of the Epha4 gene arose on the C57BL/6J inbred strain as a spontaneous recessive mutation at the Jackson Laboratory in 1997 when that inbred was at F200. This strain has been maintained primarily by breeding homozygous females with heterozygous males. However, because homozygous females are not good breeders and often do not care for their pups well, ovarian transplantation has been used on occasion to maintain this strain. In 2004 this strain reached F200+F18.
| Control | ||
|---|---|---|
| Heterozygote from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying Cdh23ahl allele
001137 129P1/ReJ 000690 129P3/J 000691 129X1/SvJ 000646 A/J 000647 A/WySnJ 003070 ALR/LtJ 003072 ALS/LtJ 004502 B6;AKR-Lxl2/GrsrJ 001026 BALB/cByJ 000653 BUB/BnJ 005494 C3.129S1(B6)-Grm1rcw/J 000664 C57BL/6J 004764 C57BL/6J-Cdh23v-8J/J 004820 C57BL/6J-Kcne12J/J 004703 C57BL/6J-Kcnq2Nmf134/J 004811 C57BL/6J-nmf110/J 004812 C57BL/6J-nmf111/J 004747 C57BL/6J-nmf118/J 004656 C57BL/6J-nmf88/J 004391 C57BL/6J-Chr 13A/J/NaJ 004385 C57BL/6J-Chr 7A/J/NaJ 000662 C57BLKS/J 000667 C57BR/cdJ 000668 C57L/J 000669 C58/J 000657 CE/J 000670 DBA/1J 001140 DBA/1LacJ 000671 DBA/2J 007048 DBA/2J-Gpnmb+/SjJ 002106 KK/HlJ 000675 LG/J 000676 LP/J 000677 MA/MyJ 001976 NOD/ShiLtJ 002050 NOR/LtJ 000679 P/J 002747 SENCARB/PtJ 002335 SKH2/J 003392 STOCK Crb1rd8/J View Strains carrying Cdh23ahl (40 strains)
Strains carrying other alleles of Cdh23
002756 B6.CAST-Cdh23Ahl+/Kjn 002432 B6J x B6.C-H2-Kbm1/ByJ-Cdh23v-J/J 002552 C57BL/6J-Cdh23v-2J/J 004764 C57BL/6J-Cdh23v-8J/J 004819 C57BL/6J-Cdh23v-9J/J 005016 CByJ;B6-Cdh23v-10J/J 000275 V/LeJ View Strains carrying other alleles of Cdh23 (7 strains)
Strains carrying other alleles of Epha4
000938 B6 x STOCK Epha4rb/J 001502 C3Sn.B6-Epha4rb/EiGrsrJ View Strains carrying other alleles of Epha4 (2 strains)
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
Epha4rb-2J/Epha4rb-2J
C57BL/6J
- behavior/neurological phenotype
- abnormal grip strength (MGI Ref ID J:87289)
- mutants show a reduced ability to hold onto an edge
- forelimbs show normal strength
- abnormal locomotor activity (MGI Ref ID J:87289)
- unlike the less severe rb mutant, rb-2J mutant mice lean frequently to either side
- abnormal gait (MGI Ref ID J:87289)
- mutant mice can be identified by 2.5-3 weeks of age by a constant hopping gait of the hindlimbs
- the forelimbs may show a hopping gait or move normally
- abnormal maternal nurturing (MGI Ref ID J:87289)
- females are poor at nurturing their young
- limb grasping (MGI Ref ID J:87289)
- mice grasp their hind limbs when picked up by the tail
- endocrine/exocrine gland phenotype
- cryptorchism (MGI Ref ID J:87289)
- males sometimes have undescended testes and fail to breed
- reproductive system phenotype
- cryptorchism (MGI Ref ID J:87289)
- males sometimes have undescended testes and fail to breed
- decreased litter size (MGI Ref ID J:87289)
- females produce small litters
- reduced male fertility (MGI Ref ID J:87289)
- due to undescended testes
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Cdh23ahl related
Epha4rb-2J relatedNeurobiology Research
Vestibular and Hearing Defects
Age related hearing loss
Sensorineural Research
Vestibular and Hearing Defects
Age related hearing loss
Neurobiology Research
Ataxia (Movement) Defects
| Allele Symbol | Epha4rb-2J | ||
|---|---|---|---|
| Allele Name | rabbit 2 Jackson | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Epha4, Eph receptor A4 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | 2900005C20Rik; AI385584; Cek8; HEK8; RGD1560587; RIKEN cDNA 2900005C20 gene; SEK; Sek; Sek1; TYRO1; expressed sequence AI385584; rabbit; rb; segmentation receptor tyrosine kinase; | ||
| Molecular Note | The rb2J mouse arose in 1997 at the Jackson Laboratory. This mouse was identified as an allele at the Epha4 locus based complementation analysis using the Epha4Gt(pGT1TM)38Wcs allele. The mutation at the level of the sequence has not been reported. [MGI Ref ID J:87289] | ||
| Allele Symbol | Cdh23ahl | ||
| Allele Name | age related hearing loss 1 | ||
| Allele Type | QTL | ||
| Common Name(s) | Cdh23753A; mdfw; | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
Cook SA; Eicher EM; Samples RM; Bronson RT; Davisson MT. 2004. Two spontaneous mutant alleles, rb and rb-2J, of the Epha4 gene on mouse Chromosome 1. Mouse Mutant Resources Web Site, The Jackson Laboratory, Bar Harbor, Maine (http://www.jax.org/mmr) MGI Direct Data Submission :. [MGI Ref ID J:87289]
Colony Maintenance
Breeding & Husbandry Homozygous males do not breed well. Most homozygous females do not breed well or rear their young well.
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Important Note | |
| The C57BL/6J background strain is homozygous for the age related hearing loss mutation Cdh23ahl, which on this background causes progressive hearing loss with onset after 10 months of age. | |
| Control | ||
|---|---|---|
| Heterozygote from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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