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Strain Name:

C57BL/6-Tg(TRAMP)8247Ng/J

Stock Number:

003135

Availability:

Repository- Live

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Genes & Alleles   Pb;   TAg;   Tg(TRAMP)8247Ng;

Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHemizygote x Inbred         (Female x Male)
Specieslaboratory mouse
Donating Investigator Norman Greenberg,   Fred Hutchinson Cancer Research Center
GenerationN?+12pN2 (08-JAN-08)

Appearance
black
Related Genotype: a/a

Strain Description
Mice carrying the (TRAMP) transgene develop progressive forms of prostate cancer with distant site metastasis. Transgenic mice exhibit various forms of disease from mild intraepithelial hyperplasia to large multinodular malignant neoplasia. Tumors have been detected in the prostate as early as 10 weeks of age. The tumors have elevated levels of nuclear TRP53 and decreased androgen receptor expression.

Strain Development
This mutant strain was developed in the laboratory of Dr. Norman M. Greenberg at Baylor College of Medicine. SV40 early region transgene was injected into C57BL/6 embryos. Rat probasin is the promoter.

Related Disease (OMIM) Terms

Prostate Cancer
Mammalian Phenotype Terms assigned by genotype

Tg(TRAMP)8247Ng/0

        involves: C57BL/6
  • tumorigenesis
  • prostate tumor (MGI Ref ID J:65303)
    • males display focal and invasive adenocarcinoma of the prostate by 20 weeks
  • reproductive system phenotype
  • prostate gland hyperplasia (MGI Ref ID J:65303)
    • intraepithelial hyperplasia can be detected in males by 10 weeks of age and by 22 weeks of age, almost all prostatic glands are hyperplastic
  • endocrine/exocrine gland phenotype
  • prostate gland hyperplasia (MGI Ref ID J:65303)
    • intraepithelial hyperplasia can be detected in males by 10 weeks of age and by 22 weeks of age, almost all prostatic glands are hyperplastic

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(TRAMP)8247Ng/0

        involves: 129S1/Sv * C57BL/6
  • tumorigenesis
  • prostate tumor (MGI Ref ID J:108849)

Tg(TRAMP)8247Ng/0

        involves: C3H * C57BL/6
  • endocrine/exocrine gland phenotype
  • increased prostate weight (MGI Ref ID J:125633)
    • prostates weigh an average of >6g, while in transgenic mice expressing the Tg(ACTB-SAC/EGFP)35Rang transgene, most (78.5%) prostate glands weigh <2g and remaining 21.5% weigh between 2 and 3 grams
  • reproductive system phenotype
  • increased prostate weight (MGI Ref ID J:125633)
    • prostates weigh an average of >6g, while in transgenic mice expressing the Tg(ACTB-SAC/EGFP)35Rang transgene, most (78.5%) prostate glands weigh <2g and remaining 21.5% weigh between 2 and 3 grams
  • tumorigenesis
  • prostate tumor (MGI Ref ID J:125633)
    • majority (62.5-83.3%) of mice develop high-grade prostatic intraepithelial neoplasia by 3 months of age and 12-16% of animals show adenocarcinoma of the prostate; by 6 months, all mice develop adenocarcinoma of the prostate

Tg(TRAMP)8247Ng/0

        involves: 129/Sv * C57BL/6
  • tumorigenesis
  • prostate tumor (MGI Ref ID J:133908)
    • mice are moribund with prostrate tumors on average at 31.4 weeks of age
    • intraepithelial neoplasia and adenocarcinomas at different stages of differentiation are observed in the prostrate

Gene & Allele Details

Allele Symbol Tg(TRAMP)8247Ng
Allele Name transgene insertion 8247, Norman M Greenberg
Common Name(s) TRAMP;
Mutation Made By Norman Greenberg,   Fred Hutchinson Cancer Research Center
Strain of OriginC57BL/6
Expressed Gene TAg, SV40 large T-antigen, SV40
Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter Pb, probasin, rat
General Note TRAMP stands for TRansgenic Adenocarcinoma Mouse Prostate. Lines 5666 and 8079 were also produced.

Transgenic mice on a C57BL6 background develop progressive forms of prostate cancer with distant site metastasis. Forms ofdisease range from mild intraepithelial hyperplasia to large multinodular malignant neoplasia. Tumors are detected in the prostate as early as 10 weeks of age. The tumors have elevated levels of nuclear TRP53 and decreased androgen receptor expression.

Molecular Note Expression of the SV40 early region tumor antigen was driven in mouse prostrate epithelium via a rat probasin promoter region fragment (-426/+28 bp). [MGI Ref ID J:65303] [MGI Ref ID J:73560]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(TRAMP)8247Ng

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Tg(TRAMP)8247Ng allele
008215   (C57BL/6-Tg(TRAMP)8247Ng/J X FVB/NJ)F1/J
View Strains carrying   Tg(TRAMP)8247Ng     (1 strain)

View Strains carrying other alleles of TAg     (12 strains)

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Prostate Tumors)

Reproductive Biology Research
Prostate Tumors

TAg related

Apoptosis Research
Extracellular Modulators

References

Selected Reference(s)

Greenberg NM; DeMayo F; Finegold MJ; Medina D; Tilley WD; Aspinall JO; Cunha GR; Donjacour AA; Matusik RJ; Rosen JM. 1995. Prostate cancer in a transgenic mouse. Proc Natl Acad Sci U S A 92(8):3439-43. [PubMed: 7724580]  [MGI Ref ID J:65303]

Additional References

Price and Supply Information

Strain Name: C57BL/6-Tg(TRAMP)8247Ng/J
Stock Number: 003135

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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