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Genes & Alleles

Gabrg2;   Gabrg2tm1Geh;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Gregg Homanics,   University of Pittsburgh Generation F3N1F4p+N1 (09-NOV-05)

Strain Description
The gamma subunit of the gamma aminobutyric acid type A receptor is essential for bestowing both normal single channel conductance and sensitivity to benzoidiazepines. Alternate RNA splicing produces two alternative forms of the gamma2 subunit from Gabrg2. The two forms, gamma2L and gamma2S are expressed in different brain regions. This strain is a knockout of the gamma2L form. Mice homozygous for this exon deletion are viable and superficially indistinguishable from wild type mice. Behaviorally, gamma2L knockout mice show increased levels of anxiety in the elevated plus maze relative to controls and also longer sleep time than control mice in response to midazolam and zolpidem.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).

Strain Development
The knockout was created through the replacement of a 0.6 kb XbaI fragment which includes the 24 bp exon 9 and associated splice signals with the bicistronic selectable marker gene (PGKneoNTRtkpA). The 129X1/SvJ x 129S1/Sv-derived ES (embryonic stem) cell line was used.

Gene & Allele Details

Allele Symbol		Gabrg2tm1Geh
Allele Name		targeted mutation 1, Gregg E Homanics
Common Name(s)		Gabrgamma2Ltm1Geh;
Mutation Made By		Gregg Homanics,   University of Pittsburgh
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl+
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Gabrg2, gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
Chromosome		11
Gene Common Name(s)		AI851231; BB128510; CAE2; ECA2; GABAA-R; GEFSP3; Gabrg-2; expressed sequence AI851231; expressed sequence BB128510; gamma2;
Molecular Note		A neomycin selection cassette replaced a genomic fragment containing exon 9. RT-PCR experiments demonstrated that this mutation resulted in the elimination of the expression of the long splice variant but not the short splice variant. Radioligand binding assays confirmed that no functional long variant was expressed from this allele. [MGI Ref ID J:53823]

Control Information

Allele	Control
Gabrg2tm1Geh	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Gabrg2L^tm1Geh

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Receptor Defects (GABA receptor)

Gabrg2^tm1Geh related

Neurobiology Research
Receptor Defects

Research Tools
Neurobiology Research

References

Selected Reference(s)

Homanics GE; Harrison NL; Quinlan JJ; Krasowski MD; Rick CE; de Blas AL ; Mehta AK ; Kist F ; Mihalek RM ; Aul JJ ; Firestone LL. 1999. Normal electrophysiological and behavioral responses to ethanol in mice lacking the long splice variant of the gamma2 subunit of the gamma-aminobutyrate type A receptor. Neuropharmacology 38(2):253-65. [PubMed: 10218866]  [MGI Ref ID J:53823]

Additional References

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Price and Supply Information

Strain Name:	B6;129-Gabrg2tm1Geh/J
Stock Number:	003137

Price Details

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Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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