Strain Name: |
B6;129-Gabrg2tm1Geh/J |
|---|---|
Stock Number: |
003137 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Gabrg2; Gabrg2tm1Geh; |
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Gregg Homanics, University of Pittsburgh Generation F3N1F4p+N1 (09-NOV-05) Strain Description
The gamma subunit of the gamma aminobutyric acid type A receptor is essential for bestowing both normal single channel conductance and sensitivity to benzoidiazepines. Alternate RNA splicing produces two alternative forms of the gamma2 subunit from Gabrg2. The two forms, gamma2L and gamma2S are expressed in different brain regions. This strain is a knockout of the gamma2L form. Mice homozygous for this exon deletion are viable and superficially indistinguishable from wild type mice. Behaviorally, gamma2L knockout mice show increased levels of anxiety in the elevated plus maze relative to controls and also longer sleep time than control mice in response to midazolam and zolpidem.Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
Strain Development
The knockout was created through the replacement of a 0.6 kb XbaI fragment which includes the 24 bp exon 9 and associated splice signals with the bicistronic selectable marker gene (PGKneoNTRtkpA). The 129X1/SvJ x 129S1/Sv-derived ES (embryonic stem) cell line was used.
| Allele Symbol | Gabrg2tm1Geh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Gregg E Homanics | ||
| Common Name(s) | Gabrgamma2Ltm1Geh; | ||
| Mutation Made By | Gregg Homanics, University of Pittsburgh | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gabrg2, gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI851231; BB128510; CAE2; ECA2; GABAA-R; GEFSP3; Gabrg-2; expressed sequence AI851231; expressed sequence BB128510; gamma2; | ||
| Molecular Note | A neomycin selection cassette replaced a genomic fragment containing exon 9. RT-PCR experiments demonstrated that this mutation resulted in the elimination of the expression of the long splice variant but not the short splice variant. Radioligand binding assays confirmed that no functional long variant was expressed from this allele. [MGI Ref ID J:53823] | ||
| Allele | Control | |
|---|---|---|
| Gabrg2tm1Geh | 101045 B6129SF2/J | (approximate) |
| Considerations for Choosing Controls | ||
Gabrg2Ltm1Geh
Gabrg2tm1Geh relatedNeurobiology Research
Receptor Defects (GABA receptor)
Neurobiology Research
Receptor Defects
Research Tools
Neurobiology Research
Selected Reference(s)
Additional ReferencesHomanics GE; Harrison NL; Quinlan JJ; Krasowski MD; Rick CE; de Blas AL ; Mehta AK ; Kist F ; Mihalek RM ; Aul JJ ; Firestone LL. 1999. Normal electrophysiological and behavioral responses to ethanol in mice lacking the long splice variant of the gamma2 subunit of the gamma-aminobutyrate type A receptor. Neuropharmacology 38(2):253-65. [PubMed: 10218866] [MGI Ref ID J:53823]
| Strain Name: | B6;129-Gabrg2tm1Geh/J |
| Stock Number: | 003137 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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