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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
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Description
Strain Information
Former Names B6.Cg-TgN(DBHnlacZ)8Rpk (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6J Donor Strain B6;SJL Generation N?+10pN1 Donating Investigator Raj Kapur, University of Washington Medical Center Appearance
black
Related Genotype: a/aDescription
Transgenic mice carry a beta-galactosidase reporter gene driven by dopamine beta hydroxylase promotor. LacZ expression is seen in neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. LacZ expression is also observed in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei.Development
A DBH-lac fusion gene was made and tested intransgenic mice. A fragment of the human DBH gene, extending from an Smal site 5.8 kb upstream from the transcription start to an Hpall site 41 bp into the 5' untranslated region of exon 1, was fused to the cloned E. coli lacZ gene of placF. This strain is identified as line DBH-nlacZ(2860-8)in the primary publication.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (178 strains)
004996 B6.Cg-Tg(DBH-Gal)1923Stei/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
lacZ relatedNeurobiology Research
lacZ expression in neural tissue
Parkinson's Disease
Research Tools
lacZ Expression
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(DBHn-lacZ)8Rpk | ||
|---|---|---|---|
| Allele Name | transgene insertion 8, Raj P Kapur | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | DBH-lacZ; DBH-nlacZ(2860-8); | ||
| Strain of Origin | C57BL/6 x SJL | ||
| Site of Expression | CNS expression. Specifically, neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. Also in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | DBH, dopamine beta-hydroxylase (dopamine beta-monooxygenase), human | ||
| General Note |
This line was generated from founder 2860-8. LacZ expression is seen in neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. LacZ expression is also observed in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei. | ||
| Molecular Note | A fragment of the human dopamine beta hydroxylase (DBH) gene, extending from a Sma1 site 5.8 kb upstream from the transcription start to an HpaII site 41 bp into the 5' untranslated region of exon 1, was fused to a bacterial beta-galactosidase reporter gene. [MGI Ref ID J:92929] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Mercer EH; Hoyle GW; Kapur RP; Brinster RL; Palmiter RD. 1991. The dopamine beta-hydroxylase gene promoter directs expression of E. coli lacZ to sympathetic and other neurons in adult transgenic mice. Neuron 7(5):703-16. [PubMed: 1742021] [MGI Ref ID J:92929]
Additional References
Tg(DBHn-lacZ)8Rpk relatedAnderson RB; Stewart AL; Young HM. 2006. Phenotypes of neural-crest-derived cells in vagal and sacral pathways. Cell Tissue Res 323(1):11-25. [PubMed: 16133146] [MGI Ref ID J:105292]
Anderson RB; Turner KN; Nikonenko AG; Hemperly J; Schachner M; Young HM. 2006. The cell adhesion molecule l1 is required for chain migration of neural crest cells in the developing mouse gut. Gastroenterology 130(4):1221-32. [PubMed: 16618414] [MGI Ref ID J:124919]
Bates MD; Dunagan DT; Welch LC; Kaul A; Harvey RP. 2006. The Hlx homeobox transcription factor is required early in enteric nervous system development. BMC Dev Biol 6:33. [PubMed: 16854219] [MGI Ref ID J:119681]
Van de Putte T; Francis A; Nelles L; van Grunsven LA; Huylebroeck D. 2007. Neural crest-specific removal of Zfhx1b in mouse leads to a wide range of neurocristopathies reminiscent of Mowat-Wilson syndrome. Hum Mol Genet 16(12):1423-36. [PubMed: 17478475] [MGI Ref ID J:125106]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;SJL background and is maintained by mating heterozygotes to C57BL/6J. Reproduction is excellent. Coat color expected from breeding:Black Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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