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Strain Name:

B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J

Stock Number:

003147

Availability:

Repository-Cryopreserved

Price and Supply Information

General Terms and Conditions

Former Name      B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Loh/J    (Changed: 15-DEC-04 )
Genes & Alleles   H2-T18;   H2;   H2d;   Hc;   Hc1;   Tcra;   Tcrb;   Tg(DO11.10)10Dlo;   ;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Major Histocompatibility Congenic
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Background Strain B10.D2-Hc1 H2d H2-T18c/nSnJ
Donor Strain BALB/c-TgN(DO11.10)10Loh
Donating Investigator Dennis Loh,   Hoffman LaRoche
GenerationN16p

Appearance
black
Related Genotype: a/a

Strain Description
Clonal deletion of autoreactive T cells in vivo was studied using a peptide antigen to induce deletion of antigen-reactive thymocytes. Mice transgenic for a T cell receptor that reacts to this peptide (Tg(DO11.10)10Loh) contain thymocytes that progress from the immature to the mature phenotype. Intraperitoneal administration of the peptide antigen to transgenic mice results in a rapid deletion of the immature CD4+ CD8+ TCRlo thymocytes. Apoptosis of cortical thymocytes can be seen within 20 hours of treatment. These results provide direct evidence for the in vivo role of apoptosis in the development of antigen-induced tolerance.

Strain Development
A transgenic construct containing the rearranged Vbeta 8.1 and Valpha 13 T cell receptor genes of chicken ovalbumin–specific T cell hybridoma DO11.10 was injected into fertilized B6C3F1 x BALB/c oocytes by Dr. Ken Murphy in the laboratory of Dr. Dennis Loh at Washington University, St. Louis in 1989. The founder animal was backcrossed to BALB/c 5-6 times before being transferred to NIH and crossed to B10.D2 for seven generations. The strain was donated to The Jackson Laboratory in 1998 where it was crossed once more with an NIH-supplied B10.D2 mouse. The offspring were mated to B10.D2-Hc1 H2d H2-T18c/nSnJ (Stock No. 000463) for 4 additional generations before being made homozygous.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(DO11.10)10Dlo/?

        Background Not Specified
  • immune system phenotype
  • abnormal thymus cellularity (MGI Ref ID J:70572)
    • 20 hours after administration of OVA323-339 peptide, apoptosis, DNA degradation, chromatin condensation, and cell shrinkage occur in the majority of cortical thymocytes
    • apoptosis occurs throughout the thymus including the subcapsular and deep cortical areas
    • 3 days after administration of peptide the cortical thymus is acellular; however, normal cellularity exists in the medulla
    • peptide administration does not effect small number of thymocytes that express only the beta chain transgene Vb8
  • decreased CD4-positive T cell number (MGI Ref ID J:70572)
    • CD4+ CD8- TCR thymocytes are reduced in absolute numbers after administration of OVA 323-339 peptide
  • decreased double-positive T cell number (MGI Ref ID J:70572)
    • CD4+CD8+TCR cells in thymic cortex are reduced by 55% after administration of OVA 323-339 peptide
  • hematopoietic system phenotype
  • abnormal thymus cellularity (MGI Ref ID J:70572)
    • 20 hours after administration of OVA323-339 peptide, apoptosis, DNA degradation, chromatin condensation, and cell shrinkage occur in the majority of cortical thymocytes
    • apoptosis occurs throughout the thymus including the subcapsular and deep cortical areas
    • 3 days after administration of peptide the cortical thymus is acellular; however, normal cellularity exists in the medulla
    • peptide administration does not effect small number of thymocytes that express only the beta chain transgene Vb8
  • decreased CD4-positive T cell number (MGI Ref ID J:70572)
    • CD4+ CD8- TCR thymocytes are reduced in absolute numbers after administration of OVA 323-339 peptide
  • decreased double-positive T cell number (MGI Ref ID J:70572)
    • CD4+CD8+TCR cells in thymic cortex are reduced by 55% after administration of OVA 323-339 peptide

Gene & Allele Details

Allele Symbol H2d
Allele Name d variant
Gene Symbol and Name H2, histocompatibility-2, MHC
Chromosome 17
Gene Common Name(s) H-2; MHC-II;
General Note The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB
 
Allele Symbol Hc1
Allele Name sufficient
Common Name(s) C5 sufficient; C5-s; C5-suf;
Gene Symbol and Name Hc, hemolytic complement
Chromosome 2
Gene Common Name(s) C5; C5a; CPAMD4; FLJ17816; FLJ17822; He; MGC142298; RGD1561905;
General Note

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The C3H/HeJ allele does not have the 2 bp deletion and is associated with resistance to allergen-induced bronchial hyperresponsiveness. (J:108211)

Molecular Note This allele does not contain the 2 base "TA" deletion found in the Hc0 allele and is found in the following mouse strains BALB/cJ, C57BL/6J, DBA/1J, and B10.D2/nSnJ. This is equivalent to the wild-type allele. [MGI Ref ID J:23983]
 
Allele Symbol Tg(DO11.10)10Dlo
Allele Name transgene insertion 10, Dennis Y Loh
Common Name(s) CO11.10 TCR-alphabeta; DO-11.10; DO-Tg; DO.10 Tg; DO.11; DO.11.10 TCR; DO11; DO11 TCR; DO11.10; DO11.10 TCR; DO11.10 TCR Tg; DO11.10 alphabeta-TCR; DO11.10TCRalphabeta; Do11.10 alphabeta TCR; Do11.10 alphabetaTCR; Tg(DO11.10)Tcra/Tcrb; TgN(DO11.10)10Dlo; TgN(DO11.10)10Loh;
Mutation Made By Dennis Loh,   Hoffman LaRoche
Expressed Gene Tcrb, T-cell receptor beta chain, mouse, laboratory
Expressed Gene Tcra, T-cell receptor alpha chain, mouse, laboratory
General Note

Mice carrying this MHC class II restricted rearranged T cell receptor transgene on a H2d background react to ovalbumin (OVA) peptide antigen. Intraperitoneal administration of OVA to transgenic mice results in a rapid deletion of the immature CD4+ CD8+ TCRlo thymocytes with progression to mature thymocytes. Apoptosis of cortical thymocytes in these mice occurs within 20 hours of treatment.

Molecular Note The transgene contains rearranged Tcra and Tcrb genes and is expressed in the majority of T cells. These rearranged transgenes encode a chicken ovalbumin-specific MHC class II (I-Ad)-restricted T cell receptor. [MGI Ref ID J:25536] [MGI Ref ID J:61321]
Gene Symbol and Name H2-T18, histocompatibility 2, T region locus 18
Chromosome 17
Gene Common Name(s) H-2T18; TL Ag; Tla; thymus leukemia antigen;

Control Information

  Control
   Noncarrier
   000463 B10.D2-Hc1 H2d H2-T18c/nSnJ
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Tg(DO11.10)10Dlo

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   H2d     (13 strains)

Strains carrying   Hc1 allele
000470   AK.M-H2m H2-T18a/nSnJ
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
000463   B10.D2-Hc1 H2d H2-T18c/nSnJ
004306   NOD.CBALs-Hc1/LtJ
View Strains carrying   Hc1     (4 strains)

Strains carrying   Tg(DO11.10)10Dlo allele
003303   C.Cg-Tg(DO11.10)10Dlo/J
View Strains carrying   Tg(DO11.10)10Dlo     (1 strain)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes (class II restricted)

Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Tcra related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Research Tools
Cancer Research (specific T cell deficiency)

Tcrb related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

H2d related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Hc1 related
Immunodeficiency (specific complement deficiency) (control)

Research Tools
Immunology and Inflammation Research (specific complement deficiency) (C5 complement) (control)

References

Selected Reference(s)

Murphy KM; Heimberger AB; Loh DY. 1990. Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo. Science 250(4988):1720-3. [PubMed: 2125367]  [MGI Ref ID J:70572]

Additional References

Price and Supply Information

Strain Name: B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
Stock Number: 003147

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)
Cryorecovery Fee $1900.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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