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Strain Name:

C.129S4-Il2rgtm1Wjl/J

Stock Number:

003169

Availability:

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Genes & Alleles   Il2rg;   Il2rgtm1Wjl;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Background Strain BALB/c
Donor Strain 129S4 via J1 ES cell line
Donating Investigator Warren Leonard,   NHLBI, NIH
GenerationF8p (30-MAY-04)

Appearance
albino
Related Genotype: Tyrc/Tyrc Tyrp1b/Tyrp1b A/A

Strain Description
Mice homozygous for the Il2rgtm1Wjl targeted mutation are viable and fertile. Mutant mice have hypoplastic thymuses with a 10-fold reduction in the absolute number of lymphocytes. They have a limited number of mature splenic B and T cells, lack NK cells and Peyer's patches, and development of gut-associated intraepithelial lymphocytes is diminished. In vitro studies show defects in NK activity, IL4-directed Ig class switching of thymocytes, and thymocyte mitogenic responses.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Il2rgtm1Wjl/Il2rgtm1Wjl

        B6.129S4-Il2rgtm1Wjl/J
  • immune system phenotype
  • abnormal dendritic cell physiology (MGI Ref ID J:123902)
    • in culture, dendritic cells are resistant to cytokine withdrawal-induced apoptosis
    • bone marrow-derived dendritic cells overproduce IL12 and IL10
  • abnormal spleen cellularity (MGI Ref ID J:123902)
    • reduced numbers of splenocytes
    • decrease in the proportion and absolute number of CD11c+ dendritic cells in the spleen
  • decreased myeloid dendritic cell number (MGI Ref ID J:123902)
    • the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen
  • hematopoietic system phenotype
  • abnormal spleen cellularity (MGI Ref ID J:123902)
    • reduced numbers of splenocytes
    • decrease in the proportion and absolute number of CD11c+ dendritic cells in the spleen
  • decreased myeloid dendritic cell number (MGI Ref ID J:123902)
    • the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen

Il2rgtm1Wjl/Y

        involves: 129S4/SvJae * C57BL/6
  • immune system phenotype
  • abnormal cell-mediated immunity (MGI Ref ID J:108209)
    • abnormal basophil physiology (MGI Ref ID J:24117)
      • IL-4 production by basophil/mast cell incubation is increased 10- to 45- fold
    • abnormal mast cell physiology (MGI Ref ID J:24117)
      • IL-4 production by basophil/mast cell incubation is increased 10- to 45- fold
    • abnormal thymocyte activation (MGI Ref ID J:24117)
      • marked reduction in proliferation of thymocytes in response to PMA and IL-4 stimulation
    • decreased T cell proliferation (MGI Ref ID J:24117)
      • less potent proliferation of splenic T cells to mitogen stimulation
    • decreased immunoglobulin level (MGI Ref ID J:24117)
      • concentrations of all immunoglobulins but IgM are diminished in sera of 5-week old mice
    • impaired NK cell cytolysis (MGI Ref ID J:24117)
      • no NK cytolytic acitivity detected
  • abnormal immune serum protein physiology (MGI Ref ID J:108209)
    • decreased immunoglobulin level (MGI Ref ID J:24117)
      • concentrations of all immunoglobulins but IgM are diminished in sera of 5-week old mice
    • decreased interferon-gamma secretion (MGI Ref ID J:24117)
      • IFN-gamma production by stimulated T cells is reduced by a 1000 fold
    • decreased interleukin-2 secretion (MGI Ref ID J:24117)
      • production of IL-2 by stimulated T cells is reduced by about 40%
    • decreased interleukin-4 secretion (MGI Ref ID J:24117)
      • production of IL-4 by stimulated T cells is reduced by about 40%
  • abnormal immune system organ morphology (MGI Ref ID J:108209)
    • abnormal gut-associated lymphoid tissue morphology (MGI Ref ID J:24117)
      • gut associated lymphoid tissue was absent in the small and large intestine
    • abnormal mesenteric lymph node morphology (MGI Ref ID J:24117)
      • small, with few follicles and no germinal centers
    • abnormal thymus cellularity (MGI Ref ID J:108209)
      • after 1 week of daily injections of anti-Tpte, thymic cellularity is increased 5-10 fold in null mice; injections had no effect on cellularity in double knockouts
      • thymic cortex hypoplasia (MGI Ref ID J:24117)
        • cortex is reduced in size
    • small lymph nodes (MGI Ref ID J:24117)
      • peripheral lymph nodes are not identifiable
    • small thymus (MGI Ref ID J:24117)
      • thymus is very small
      • thymic cortex hypoplasia (MGI Ref ID J:24117)
        • cortex is reduced in size
    • spleen hyperplasia (MGI Ref ID J:24117)
      • about a 2 fold increase in cellularity at nine weeks of age
    • spleen hypoplasia (MGI Ref ID J:24117)
      • about a 10 fold reduction in cellularity at three weeks of age
  • abnormal lymphocyte morphology (MGI Ref ID J:108209)
    • abnormal B cell number (MGI Ref ID J:108209)
      • after treatment with anti-Tpte, null mice show increased numbers of B cells in the spleen which contribute to the increased splenic cellularity
      • decreased immature B cell number (MGI Ref ID J:24117)
        • absolute numbers are reduced in the spleen
    • abnormal CD4-positive T cell morphology (MGI Ref ID J:108209)
      • when Tpte-deficient CD4+ T cells are injected into irradiated knockouts, transferred cells exhibit less expansion compared to wild type CD4+ T cells
    • abnormal thymocyte activation (MGI Ref ID J:24117)
      • marked reduction in proliferation of thymocytes in response to PMA and IL-4 stimulation
    • absent NK cells (MGI Ref ID J:24117)
      • NK1.1 positive cells are not detected
    • absent gamma-delta T cells (MGI Ref ID J:24117)
      • no gamma-delta T cells are found in the thymus or the skin
      • absent gamma-delta intraepithelial T cell (MGI Ref ID J:24117)
        • no gamma-delta IEL are found in the intestine
    • decreased T cell proliferation (MGI Ref ID J:24117)
      • less potent proliferation of splenic T cells to mitogen stimulation
    • decreased double-negative T cell number (MGI Ref ID J:24117)
      • there is a reduced number of double-positive T cells in the thymus
    • decreased double-positive T cell number (MGI Ref ID J:24117)
      • there is a reduced number of double-negative T cells in the thymus
    • decreased single-positive T cell number (MGI Ref ID J:24117)
      • there is a reduced number of single-postive T cells in the thymus
      • decreased CD8-positive T cell number (MGI Ref ID J:24117)
        • 10- to 20- fold drop in percentage of CD8 positive T cells found in three week old spleen
        • percentages of CD8 positive T cells in spleen return to normal by 9 weeks of age
  • digestive/alimentary phenotype
  • abnormal cecum morphology (MGI Ref ID J:24117)
    • crypt height is increased and inflammation is visible in the lamina propia
  • hematopoietic system phenotype
  • abnormal lymphocyte morphology (MGI Ref ID J:108209)
    • abnormal B cell number (MGI Ref ID J:108209)
      • after treatment with anti-Tpte, null mice show increased numbers of B cells in the spleen which contribute to the increased splenic cellularity
      • decreased immature B cell number (MGI Ref ID J:24117)
        • absolute numbers are reduced in the spleen
    • abnormal CD4-positive T cell morphology (MGI Ref ID J:108209)
      • when Tpte-deficient CD4+ T cells are injected into irradiated knockouts, transferred cells exhibit less expansion compared to wild type CD4+ T cells
    • abnormal thymocyte activation (MGI Ref ID J:24117)
      • marked reduction in proliferation of thymocytes in response to PMA and IL-4 stimulation
    • absent NK cells (MGI Ref ID J:24117)
      • NK1.1 positive cells are not detected
    • absent gamma-delta T cells (MGI Ref ID J:24117)
      • no gamma-delta T cells are found in the thymus or the skin
      • absent gamma-delta intraepithelial T cell (MGI Ref ID J:24117)
        • no gamma-delta IEL are found in the intestine
    • decreased T cell proliferation (MGI Ref ID J:24117)
      • less potent proliferation of splenic T cells to mitogen stimulation
    • decreased double-negative T cell number (MGI Ref ID J:24117)
      • there is a reduced number of double-positive T cells in the thymus
    • decreased double-positive T cell number (MGI Ref ID J:24117)
      • there is a reduced number of double-negative T cells in the thymus
    • decreased single-positive T cell number (MGI Ref ID J:24117)
      • there is a reduced number of single-postive T cells in the thymus
      • decreased CD8-positive T cell number (MGI Ref ID J:24117)
        • 10- to 20- fold drop in percentage of CD8 positive T cells found in three week old spleen
        • percentages of CD8 positive T cells in spleen return to normal by 9 weeks of age
  • abnormal thymus cellularity (MGI Ref ID J:108209)
    • after 1 week of daily injections of anti-Tpte, thymic cellularity is increased 5-10 fold in null mice; injections had no effect on cellularity in double knockouts
    • thymic cortex hypoplasia (MGI Ref ID J:24117)
      • cortex is reduced in size
  • small thymus (MGI Ref ID J:24117)
    • thymus is very small
    • thymic cortex hypoplasia (MGI Ref ID J:24117)
      • cortex is reduced in size
  • spleen hyperplasia (MGI Ref ID J:24117)
    • about a 2 fold increase in cellularity at nine weeks of age
  • spleen hypoplasia (MGI Ref ID J:24117)
    • about a 10 fold reduction in cellularity at three weeks of age

Gene & Allele Details

Allele Symbol Il2rgtm1Wjl
Allele Name targeted mutation 1, Warren J Leonard
Common Name(s) CD132-; IL2Rgammanull; [KO]gammac; gammac-;
Mutation Made By Warren Leonard,   NHLBI, NIH
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Il2rg, interleukin 2 receptor, gamma chain
Chromosome X
Gene Common Name(s) Ab2-183; CD132; IMD4; SCIDX; SCIDX1; [g]c; common cytokine receptor gamma chain; common gamma chain; gamma C receptor; gamma(c);
Molecular Note A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein. [MGI Ref ID J:24117]

Control Information

  Control
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Il2rgtm1Wjl

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Il2rgtm1Wjl allele
003174   B6.129S4-Il2rgtm1Wjl/J
005557   NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
007799   NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ
View Strains carrying   Il2rgtm1Wjl     (3 strains)

Strains carrying other alleles of Il2rg
002479   STOCK Il2rgtm1Cgn/J
View Strains carrying other alleles of Il2rg     (1 strain)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Inflammation (Inflammatory bowel disease)

Research Tools
Immunology and Inflammation Research (NK Cell Deficiency)

Il2rgtm1Wjl related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

References

Selected Reference(s)

Cao X; Shores EW; Hu-Li J; Anver MR; Kelsall BL; Russell SM; Drago J; Noguchi M; Grinberg A; Bloom ET; Paul WE; Katz SI; Love PE; Leonard WJ. 1995. Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain. Immunity 2(3):223-38. [PubMed: 7697543]  [MGI Ref ID J:24117]

Additional References

Price and Supply Information

Strain Name: C.129S4-Il2rgtm1Wjl/J
Stock Number: 003169

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryopreserved Embryos
This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page.
Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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