Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+9+N1p (12-JUN-05) Generation Definitions   Donating Investigator Dr. Manfred Kopf,   ETH Zurich

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Il5^tm1Kopf targeted mutation are viable and fertile. Surprisingly, IL5 deficient mice show no defects in conventional B (B-2) cells, T cell dependent antibody production, or cytotoxic T cell responses. Eosinophilia response to infection is lacking in homozygous mutant mice. They also exhibit impaired CD5⁺ B-1 cell development.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Il5^tm1Kopf/Il5^tm1Kopf

        C57BL/6-Il5^tm1Kopf/J

• immune system phenotype
• increased eosinophil cell number
  • knockout mice infected with parasites do not show eosinophil counts above baseline values, while 35-week old controls develop eosinophilia in the blood, bone marrow and peritoneal cavity   (MGI Ref ID J:30867)
• hematopoietic system phenotype
• increased eosinophil cell number
  • knockout mice infected with parasites do not show eosinophil counts above baseline values, while 35-week old controls develop eosinophilia in the blood, bone marrow and peritoneal cavity   (MGI Ref ID J:30867)

Il5^tm1Kopf/Il5^tm1Kopf

        C57BL/6-Il5^tm1Kopf

• respiratory system phenotype
• decreased airway responsiveness
  • airways of aeroallergen-challenged knockout mice lose hyperresponsiveness to methacholine   (MGI Ref ID J:30641)
• immune system phenotype
• granulomatous inflammation
  • pulmonary granulomas that form in knockout mice in response to schistosome eggs are almost completely devoid of eosinophils compared to granulomas in wild-type mice; the average size of granulomas in knockout mice are 40% smaller than those of wild-type   (MGI Ref ID J:107418)
  • there is a 20-25% increase in numbers of macrophages associated with granulomas in knockouts compared with wild-type; with acute or chronic parasite infection, the numbers of both fibroblasts and macrophages nearly double in knockout animals compared to wild type   (MGI Ref ID J:107418)
• increased interferon-gamma secretion
  • in mesenteric lymph nodes in culture, there is a marked increase in IFNG production in knockouts compared to wild-type after restimulation with mitogen or SEA   (MGI Ref ID J:107418)
• increased lymphocyte cell number
  • after aerosolized OVA challenge, knockout mice have increased levels of lymphocytes in BALF compared to unchallenged animals but the increase is less pronounced than in wild-type mice   (MGI Ref ID J:30641)
• increased neutrophil cell number
  • after aerosolized OVA challenge, knockout mice have increased levels of neutrophils in BALF compared to unchallenged animals   (MGI Ref ID J:30641)
• liver/biliary system phenotype
• liver fibrosis
  • hepatic fibrosis is significantly reduced in knockouts with acute or chronic helminth parasite infection   (MGI Ref ID J:107418)
• hematopoietic system phenotype
• increased lymphocyte cell number
  • after aerosolized OVA challenge, knockout mice have increased levels of lymphocytes in BALF compared to unchallenged animals but the increase is less pronounced than in wild-type mice   (MGI Ref ID J:30641)
• increased neutrophil cell number
  • after aerosolized OVA challenge, knockout mice have increased levels of neutrophils in BALF compared to unchallenged animals   (MGI Ref ID J:30641)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Il5^tm1Kopf/Il5^tm1Kopf

        either: C57BL/6-Il5^tm1Kopf or (involves: BALB/c * C57BL/6) or (involves: C57BL/6 * CBA)

• embryogenesis phenotype
• enlarged placenta
  • knockout females have a significant increase in placenta size in matings with CBA males and the fetal:placental ratio is reduced   (MGI Ref ID J:65853)
• reproductive system phenotype
• prolonged estrous cycle
  • duration of oestrus in knockouts is moderately extended (5.6 days versus 5 days in controls)   (MGI Ref ID J:65853)
• behavior/neurological phenotype
• abnormal mating receptivity
  • duration of mating interval to achieve mating with syngeneic (B6) versus allogeneic (CBA or BALB/c) males is increased   (MGI Ref ID J:65853)
  • after correction for male background, knockout females have significantly lower mating intervals than control females   (MGI Ref ID J:65853)
• growth/size phenotype
• increased body weight
  • allogeneic matings produce larger pups than syngeneic pups; in CBA F1 pups, at weaning, female and male weights are increased 9.5 and 10%, while in C57BL/6 F1 pups, female and male weights at 6 weeks are increased by 7.9 and 12.2%   (MGI Ref ID J:65853)
  • Il5 deficient pups have an increased weight gain trajectory compared to wild-type;   (MGI Ref ID J:65853)
  • knockout pups are significantly larger than wild-type pups from birth to adulthood   (MGI Ref ID J:65853)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Il5^tm1Kopf related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Il5tm1Kopf
Allele Name		targeted mutation 1, Manfred A Kopf
Allele Type		Targeted (knock-out)
Common Name(s)		IL-5-; IL-5null;
Mutation Made By		Dr. Manfred Kopf,   ETH Zurich
Strain of Origin		C57BL/6J
ES Cell Line Name		BL/6-III
ES Cell Line Strain		C57BL/6J
Gene Symbol and Name		Il5, interleukin 5
Chromosome		11
Gene Common Name(s)		EDF; IL-5; Il-5; TRF;
Molecular Note		A neomycin resistance cassette was inserted into exon 3 of the gene. [MGI Ref ID J:30867]

Genotyping

Genotyping Information

Genotyping Protocols

Il5^tm1Kopf, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kopf M; Brombacher F; Hodgkin PD; Ramsay AJ; Milbourne EA; Dai WJ; Ovington KS; Behm CA; Kohler G; Young IG; Matthaei KI. 1996. IL-5-deficient mice have a developmental defect in CD5+ B-1 cells and lack eosinophilia but have normal antibody and cytotoxic T cell responses. Immunity 4(1):15-24. [PubMed: 8574848]  [MGI Ref ID J:30867]

Additional References

Coyle AJ; Kohler G; Tsuyuki S; Brombacher F; Kopf M. 1998. Eosinophils are not required to induce airway hyperresponsiveness after nematode infection. Eur J Immunol 28(9):2640-7. [PubMed: 9754552]  [MGI Ref ID J:50280]

Mishra A; Hogan SP; Brandt EB; Rothenberg ME. 2001. An etiological role for aeroallergens and eosinophils in experimental esophagitis. J Clin Invest 107(1):83-90. [PubMed: 11134183]  [MGI Ref ID J:66723]

Noffz G; Qin Z; Kopf M; Blankenstein T. 1998. Neutrophils but not eosinophils are involved in growth suppression of IL-4-secreting tumors. J Immunol 160(1):345-50. [PubMed: 9551990]  [MGI Ref ID J:46311]

Il5^tm1Kopf related

Ankathatti Munegowda M; Xu S; Freywald A; Xiang J. 2012. CD4+ Th2 cells function alike effector Tr1 and Th1 cells through the deletion of a single cytokine IL-6 and IL-10 gene. Mol Immunol 51(2):143-9. [PubMed: 22424785]  [MGI Ref ID J:184874]

Apostolopoulos V; McKenzie IF; Lees C; Matthaei KI; Young IG. 2000. A role for IL-5 in the induction of cytotoxic T lymphocytes in vivo Eur J Immunol 30(6):1733-9. [PubMed: 10898511]  [MGI Ref ID J:62734]

Arras M; Louahed J; Heilier JF; Delos M; Brombacher F; Renauld JC; Lison D; Huaux F. 2005. IL-9 protects against bleomycin-induced lung injury: involvement of prostaglandins. Am J Pathol 166(1):107-15. [PubMed: 15632004]  [MGI Ref ID J:95244]

Babayan SA; Read AF; Lawrence RA; Bain O; Allen JE. 2010. Filarial parasites develop faster and reproduce earlier in response to host immune effectors that determine filarial life expectancy. PLoS Biol 8(10):e1000525. [PubMed: 20976099]  [MGI Ref ID J:167364]

Bao S; Beagley KW; Murray AM; Caristo V; Matthaei KI; Young IG; Husband AJ. 1998. Intestinal IgA plasma cells of the B1 lineage are IL-5 dependent. Immunology 94(2):181-8. [PubMed: 9741339]  [MGI Ref ID J:112427]

Binder CJ; Hartvigsen K; Chang MK; Miller M; Broide D; Palinski W; Curtiss LK; Corr M; Witztum JL. 2004. IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis. J Clin Invest 114(3):427-37. [PubMed: 15286809]  [MGI Ref ID J:118092]

Brunet LR; Sabin EA; Cheever AW; Kopf MA; Pearce EJ. 1999. Interleukin 5 (IL-5) is not required for expression of a Th2 response or host resistance mechanisms during murine schistosomiasis mansoni but does play a role in development of IL-4-producing non-T, non-B cells. Infect Immun 67(6):3014-8. [PubMed: 10338513]  [MGI Ref ID J:55454]

Cenci E; Mencacci A; Del Sero G; Bacci A; Montagnoli C; d'Ostiani CF; Mosci P; Bachmann M; Bistoni F; Kopf M; Romani L. 1999. Interleukin-4 causes susceptibility to invasive pulmonary aspergillosis through suppression of protective type I responses. J Infect Dis 180(6):1957-68. [PubMed: 10558953]  [MGI Ref ID J:119841]

Cho JY; Miller M; Baek KJ; Han JW; Nayar J; Lee SY; McElwain K; McElwain S; Friedman S; Broide DH. 2004. Inhibition of airway remodeling in IL-5-deficient mice. J Clin Invest 113(4):551-60. [PubMed: 14966564]  [MGI Ref ID J:88171]

Cohn L; Homer RJ; MacLeod H; Mohrs M; Brombacher F; Bottomly K. 1999. Th2-induced airway mucus production is dependent on IL-4Ralpha, but not on eosinophils. J Immunol 162(10):6178-83. [PubMed: 10229862]  [MGI Ref ID J:119786]

Denkinger CM; Denkinger MD; Forsthuber TG. 2007. Pertussis toxin-induced cytokine differentiation and clonal expansion of T cells is mediated predominantly via costimulation. Cell Immunol 246(1):46-54. [PubMed: 17601518]  [MGI Ref ID J:123551]

Dixon H; Blanchard C; Deschoolmeester ML; Yuill NC; Christie JW; Rothenberg ME; Else KJ. 2006. The role of Th2 cytokines, chemokines and parasite products in eosinophil recruitment to the gastrointestinal mucosa during helminth infection. Eur J Immunol 36(7):1753-63. [PubMed: 16783848]  [MGI Ref ID J:115793]

Domachowske JB; Bonville CA; Easton AJ; Rosenberg HF. 2002. Pulmonary eosinophilia in mice devoid of interleukin-5. J Leukoc Biol 71(6):966-72. [PubMed: 12050181]  [MGI Ref ID J:124313]

Forbes E; Murase T; Yang M; Matthaei KI; Lee JJ; Lee NA; Foster PS; Hogan SP. 2004. Immunopathogenesis of experimental ulcerative colitis is mediated by eosinophil peroxidase. J Immunol 172(9):5664-75. [PubMed: 15100311]  [MGI Ref ID J:89683]

Foster PS; Hogan SP; Ramsay AJ; Matthaei KI; Young IG. 1996. Interleukin 5 deficiency abolishes eosinophilia, airways hyperreactivity, and lung damage in a mouse asthma model [see comments] J Exp Med 183(1):195-201. [PubMed: 8551223]  [MGI Ref ID J:30641]

Hamelmann E; Takeda K; Haczku A; Cieslewicz G; Shultz L; Hamid Q; Xing Z; Gauldie J; Gelfand EW. 2000. Interleukin (IL)-5 but not immunoglobulin E reconstitutes airway inflammation and airway hyperresponsiveness in IL-4-deficient mice. Am J Respir Cell Mol Biol 23(3):327-34. [PubMed: 10970823]  [MGI Ref ID J:114176]

Hao H; Cohen DA; Jennings CD; Bryson JS; Kaplan AM. 2000. Bleomycin-induced pulmonary fibrosis is independent of eosinophils J Leukoc Biol 68(4):515-21. [PubMed: 11037973]  [MGI Ref ID J:65583]

Herbert DR; Lee JJ; Lee NA; Nolan TJ; Schad GA; Abraham D. 2000. Role of IL-5 in innate and adaptive immunity to larval Strongyloides stercoralis in mice. J Immunol 165(8):4544-51. [PubMed: 11035095]  [MGI Ref ID J:119216]

Hofstetter HH; Sewell DL; Liu F; Sandor M; Forsthuber T; Lehmann PV; Fabry Z. 2003. Autoreactive T cells promote post-traumatic healing in the central nervous system. J Neuroimmunol 134(1-2):25-34. [PubMed: 12507769]  [MGI Ref ID J:119263]

Hogan SP; Matthaei KI; Young JM; Koskinen A; Young IG; Foster PS. 1998. A novel T cell-regulated mechanism modulating allergen-induced airways hyperreactivity in BALB/c mice independently of IL-4 and IL-5. J Immunol 161(3):1501-9. [PubMed: 9686617]  [MGI Ref ID J:48882]

Hogan SP; Mishra A; Brandt EB; Foster PS; Rothenberg ME. 2000. A critical role for eotaxin in experimental oral antigen-induced eosinophilic gastrointestinal allergy. Proc Natl Acad Sci U S A 97(12):6681-6. [PubMed: 10841566]  [MGI Ref ID J:62718]

Huaux F; Liu T; McGarry B; Ullenbruch M; Xing Z; Phan SH. 2003. Eosinophils and T lymphocytes possess distinct roles in bleomycin-induced lung injury and fibrosis. J Immunol 171(10):5470-81. [PubMed: 14607953]  [MGI Ref ID J:119211]

Hung K; Hayashi R; Lafond-Walker A; Lowenstein C; Pardoll D; Levitsky H. 1998. The central role of CD4(+) T cells in the antitumor immune response. J Exp Med 188(12):2357-68. [PubMed: 9858522]  [MGI Ref ID J:51677]

Jacobsen EA; Zellner KR; Colbert D; Lee NA; Lee JJ. 2011. Eosinophils regulate dendritic cells and Th2 pulmonary immune responses following allergen provocation. J Immunol 187(11):6059-68. [PubMed: 22048766]  [MGI Ref ID J:179701]

MacDonald AS; Loke P; Allen JE. 1999. Suppressive antigen-presenting cells in Helminth infection. Pathobiology 67(5-6):265-8. [PubMed: 10725799]  [MGI Ref ID J:61096]

Mattes J; Yang M; Mahalingam S; Kuehr J; Webb DC; Simson L; Hogan SP; Koskinen A; McKenzie AN; Dent LA; Rothenberg ME; Matthaei KI; Young IG; Foster PS. 2002. Intrinsic defect in T cell production of interleukin (IL)-13 in the absence of both IL-5 and eotaxin precludes the development of eosinophilia and airways hyperreactivity in experimental asthma. J Exp Med 195(11):1433-44. [PubMed: 12045241]  [MGI Ref ID J:116794]

McKee AS; Munks MW; MacLeod MK; Fleenor CJ; Van Rooijen N; Kappler JW; Marrack P. 2009. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183(7):4403-14. [PubMed: 19734227]  [MGI Ref ID J:152787]

Mishra A; Hogan SP; Brandt EB; Rothenberg ME. 2002. IL-5 promotes eosinophil trafficking to the esophagus. J Immunol 168(5):2464-9. [PubMed: 11859139]  [MGI Ref ID J:74724]

Mishra A; Wang M; Pemmaraju VR; Collins MH; Fulkerson PC; Abonia JP; Blanchard C; Putnam PE; Rothenberg ME. 2008. Esophageal remodeling develops as a consequence of tissue specific IL-5-induced eosinophilia. Gastroenterology 134(1):204-14. [PubMed: 18166354]  [MGI Ref ID J:135588]

Moon BG; Takaki S; Miyake K; Takatsu K. 2004. The role of IL-5 for mature B-1 cells in homeostatic proliferation, cell survival, and Ig production. J Immunol 172(10):6020-9. [PubMed: 15128785]  [MGI Ref ID J:89849]

Munks MW; McKee AS; Macleod MK; Powell RL; Degen JL; Reisdorph NA; Kappler JW; Marrack P. 2010. Aluminum adjuvants elicit fibrin-dependent extracellular traps in vivo. Blood 116(24):5191-9. [PubMed: 20876456]  [MGI Ref ID J:167390]

Noffz G; Qin Z; Kopf M; Blankenstein T. 1998. Neutrophils but not eosinophils are involved in growth suppression of IL-4-secreting tumors. J Immunol 160(1):345-50. [PubMed: 9551990]  [MGI Ref ID J:46311]

Ovington KS; McKie K; Matthaei KI; Young IG; Behm CA. 1998. Regulation of primary Strongyloides ratti infections in mice: a role for interleukin-5. Immunology 95(3):488-93. [PubMed: 9824515]  [MGI Ref ID J:51185]

Padilla J; Daley E; Chow A; Robinson K; Parthasarathi K; McKenzie AN; Tschernig T; Kurup VP; Donaldson DD; Grunig G. 2005. IL-13 regulates the immune response to inhaled antigens. J Immunol 174(12):8097-105. [PubMed: 15944318]  [MGI Ref ID J:100867]

Peebles RS Jr; Hashimoto K; Sheller JR; Moore ML; Morrow JD; Ji S; Elias JA; Goleniewska K; O'neal J; Mitchell DB; Graham BS; Zhou W. 2005. Allergen-induced airway hyperresponsiveness mediated by cyclooxygenase inhibition is not dependent on 5-lipoxygenase or IL-5, but is IL-13 dependent. J Immunol 175(12):8253-9. [PubMed: 16339565]  [MGI Ref ID J:122257]

Pochanke V; Hatak S; Hengartner H; Zinkernagel RM; McCoy KD. 2006. Induction of IgE and allergic-type responses in fur mite-infested mice. Eur J Immunol 36(9):2434-45. [PubMed: 16909433]  [MGI Ref ID J:116736]

Pope SM; Brandt EB; Mishra A; Hogan SP; Zimmermann N; Matthaei KI; Foster PS; Rothenberg ME. 2001. IL-13 induces eosinophil recruitment into the lung by an IL-5- and eotaxin-dependent mechanism. J Allergy Clin Immunol 108(4):594-601. [PubMed: 11590387]  [MGI Ref ID J:96752]

Ramalingam T; Ganley-Leal L; Porte P; Rajan TV. 2003. Impaired clearance of primary but not secondary Brugia infections in IL-5 deficient mice. Exp Parasitol 105(2):131-9. [PubMed: 14969690]  [MGI Ref ID J:102607]

Reiman RM; Thompson RW; Feng CG; Hari D; Knight R; Cheever AW; Rosenberg HF; Wynn TA. 2006. Interleukin-5 (IL-5) augments the progression of liver fibrosis by regulating IL-13 activity. Infect Immun 74(3):1471-9. [PubMed: 16495517]  [MGI Ref ID J:107418]

Roa J; Morikawa H; Crawford L; Baatjes A; Duong M; Denburg JA. 2007. The effects of montelukast on tissue inflammatory and bone marrow responses in murine experimental allergic rhinitis: interaction with interleukin-5 deficiency. Immunology 122(3):438-44. [PubMed: 17627772]  [MGI Ref ID J:127184]

Robertson SA; Mau VJ; Young IG; Matthaei KI. 2000. Uterine eosinophils and reproductive performance in interleukin 5-deficient mice. J Reprod Fertil 120(2):423-32. [PubMed: 11058459]  [MGI Ref ID J:65853]

Saito H; Matsumoto K; Denburg AE; Crawford L; Ellis R; Inman MD; Sehmi R; Takatsu K; Matthaei KI; Denburg JA. 2002. Pathogenesis of murine experimental allergic rhinitis: a study of local and systemic consequences of IL-5 deficiency. J Immunol 168(6):3017-23. [PubMed: 11884474]  [MGI Ref ID J:126473]

Schwarze J; Cieslewicz G; Hamelmann E; Joetham A; Shultz LD; Lamers MC; Gelfand EW. 1999. IL-5 and eosinophils are essential for the development of airway hyperresponsiveness following acute respiratory syncytial virus infection. J Immunol 162(5):2997-3004. [PubMed: 10072551]  [MGI Ref ID J:110844]

Sharkhuu T; Matthaei KI; Forbes E; Mahalingam S; Hogan SP; Hansbro PM; Foster PS. 2006. Mechanism of interleukin-25 (IL-17E)-induced pulmonary inflammation and airways hyper-reactivity. Clin Exp Allergy 36(12):1575-83. [PubMed: 17177681]  [MGI Ref ID J:135913]

Simons JE; Rothenberg ME; Lawrence RA. 2005. Eotaxin-1-regulated eosinophils have a critical role in innate immunity against experimental Brugia malayi infection. Eur J Immunol 35(1):189-97. [PubMed: 15593125]  [MGI Ref ID J:95225]

Spergel JM; Mizoguchi E; Oettgen H; Bhan AK; Geha RS. 1999. Roles of TH1 and TH2 cytokines in a murine model of allergic dermatitis. J Clin Invest 103(8):1103-11. [PubMed: 10207161]  [MGI Ref ID J:119611]

Swartz JM; Bystrom J; Dyer KD; Nitto T; Wynn TA; Rosenberg HF. 2004. Plasminogen activator inhibitor-2 (PAI-2) in eosinophilic leukocytes. J Leukoc Biol 76(4):812-9. [PubMed: 15277569]  [MGI Ref ID J:92977]

Temann UA; Laouar Y; Eynon EE; Homer R; Flavell RA. 2007. IL9 leads to airway inflammation by inducing IL13 expression in airway epithelial cells. Int Immunol 19(1):1-10. [PubMed: 17101709]  [MGI Ref ID J:118163]

Vallance BA; Blennerhassett PA; Deng Y; Matthaei KI; Young IG; Collins SM. 1999. IL-5 contributes to worm expulsion and muscle hypercontractility in a primary T. spiralis infection. Am J Physiol 277(2 Pt 1):G400-8. [PubMed: 10444455]  [MGI Ref ID J:56839]

Vallance BA; Matthaei KI; Sanovic S; Young IG; Collins SM. 2000. Interleukin-5 deficient mice exhibit impaired host defence against challenge Trichinella spiralis infections. Parasite Immunol 22(10):487-92. [PubMed: 11012974]  [MGI Ref ID J:103984]

Vink A; Warnier G; Brombacher F; Renauld JC. 1999. Interleukin 9-induced in vivo expansion of the B-1 lymphocyte population. J Exp Med 189(9):1413-23. [PubMed: 10224281]  [MGI Ref ID J:136446]

Wang J; Palmer K; Lotvall J; Milan S; Lei XF; Matthaei KI; Gauldie J; Inman MD; Jordana M; Xing Z. 1998. Circulating, but not local lung, IL-5 is required for the development of antigen-induced airways eosinophilia. J Clin Invest 102(6):1132-41. [PubMed: 9739047]  [MGI Ref ID J:115210]

Webb DC; Mahalingam S; Cai Y; Matthaei KI; Donaldson DD; Foster PS. 2003. Antigen-specific production of interleukin (IL)-13 and IL-5 cooperate to mediate IL-4Ralpha-independent airway hyperreactivity. Eur J Immunol 33(12):3377-85. [PubMed: 14635046]  [MGI Ref ID J:87139]

Weir C; Bernard CC; Backstrom BT. 2003. IL-5-deficient mice are susceptible to experimental autoimmune encephalomyelitis. Int Immunol 15(11):1283-9. [PubMed: 14565926]  [MGI Ref ID J:110934]

Wensky AK; Furtado GC; Marcondes MC; Chen S; Manfra D; Lira SA; Zagzag D; Lafaille JJ. 2005. IFN-gamma determines distinct clinical outcomes in autoimmune encephalomyelitis. J Immunol 174(3):1416-23. [PubMed: 15661899]  [MGI Ref ID J:110017]

Wu CA; Peluso JJ; Zhu L; Lingenheld EG; Walker ST; Puddington L. 2010. Bronchial epithelial cells produce IL-5: implications for local immune responses in the airways. Cell Immunol 264(1):32-41. [PubMed: 20494340]  [MGI Ref ID J:162110]

Yang M; Hogan SP; Henry PJ; Matthaei KI; McKenzie AN; Young IG; Rothenberg ME; Foster PS. 2001. Interleukin-13 mediates airways hyperreactivity through the IL-4 receptor-alpha chain and STAT-6 independently of IL-5 and eotaxin. Am J Respir Cell Mol Biol 25(4):522-30. [PubMed: 11694459]  [MGI Ref ID J:114421]

Zarei S; Schwenter F; Luy P; Aurrand-Lions M; Morel P; Kopf M; Dranoff G; Mach N. 2009. Role of GM-CSF signaling in cell-based tumor immunization. Blood 113(26):6658-68. [PubMed: 19282460]  [MGI Ref ID J:150155]

Zhang Y; Denkers EY. 1999. Protective role for interleukin-5 during chronic Toxoplasma gondii infection. Infect Immun 67(9):4383-92. [PubMed: 10456878]  [MGI Ref ID J:57116]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.