Strain Name:

STOCK Cdh2tm1Hyn/J

Stock Number:

003179

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Richard Hynes,   Massachusetts Institute of Technology

Description
Mice that are heterozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. However, homozygous mice have an embryonic lethal phenotype, and die by embryonic day 10. The somites of the mutant embryos are small, irregularly shaped, and less cohesive compared with those of their wild-type littermates, and the epithelial organization of the somites is partially disrupted. Undulation of the neural tube is also observed in the mutant embryos. The mesodermal and endodermal cell layers of the yolk sac are separated in the mutants. The most dramatic cell adhesion defect is observed in the primitive heart; although myocardial tissue forms initially, the myocytes subsequently dissociate and the heart tube fails to develop normally.

Development
A BamHI-BamHI 8.3 kb fragment was isolated from lambda clone, MNG16. The BamHI genomic DNA fragment was subcloned into a plasmid containing the PMC1-HSVTK cassette. The neo expression cassette, PGKneobpA, was digested with XhoI/SalI, and subcloned into a unique SalI site in exon 10. The construct was linearized with NotI and electroporated into 129S2/SvPas-derived D3 ES cells, and colonies were selected with G418 and gancyclovir. A 2.4 kb SmaI-HindIII genomic DNA fragment located immediately 3' to the short arm of the targeting vector was used as a probe. The targeted ES clones were injected into blastocysts from C57BL/6J mice.

Related Strains

Strains carrying other alleles of Cdh2
007611   B6.129S6(SJL)-Cdh2tm1Glr/J
View Strains carrying other alleles of Cdh2     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cdh2tm1Hyn/Cdh2tm1Hyn

        involves: 129S2/SvPas
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • mutant embryos die by E10   (MGI Ref ID J:37888)
  • embryogenesis phenotype
  • abnormal embryo turning
    • rarely turn completely from the lordotic to the fetal position   (MGI Ref ID J:37888)
  • abnormal somite shape
    • at E8.5, somites are small, irregularly shaped, and less cohesive   (MGI Ref ID J:37888)
    • at E9.0, epithelial somites are smaller and irregular in size and shape   (MGI Ref ID J:58409)
    • epithelial somites are cleaved into rostral and caudal halves immediately after somite formation   (MGI Ref ID J:58409)
    • however, most of these fragmented somites maintain epithelial, vesicle-like structures   (MGI Ref ID J:58409)
  • abnormal visceral yolk sac morphology
    • exhibit separation of the mesodermal and endodermal cell layers of the yolk sac; however, points of contact are still maintained between the two cell layers   (MGI Ref ID J:37888)
  • abnormal vitelline vasculature morphology
    • blood vessels in the yolk sac are not appropriately formed   (MGI Ref ID J:37888)
  • embryonic growth retardation
    • significantly delayed by E9.5 and are equivalent in size to normal E9 embryos   (MGI Ref ID J:37888)
  • wavy neural tube
    • undulation of the neural tube   (MGI Ref ID J:37888)
  • cardiovascular system phenotype
  • abnormal heart tube morphology
    • all embryos examined between E9 and E10 exhibit a malformed heart tube   (MGI Ref ID J:37888)
    • myocytes dissociated from heart tubes form loose aggregates that contract weakly in vitro compared to strong beating aggregates in wild-type   (MGI Ref ID J:37888)
  • abnormal myocardium layer morphology
    • cardiac myoocytes surround the endocardium and are unable to develop a normal myocardium   (MGI Ref ID J:37888)
    • abnormal myocardial fiber morphology
      • cardiac myocytes tend to disaggregate   (MGI Ref ID J:37888)
  • abnormal vitelline vasculature morphology
    • blood vessels in the yolk sac are not appropriately formed   (MGI Ref ID J:37888)
  • distended pericardium
    • expanded pericardial cavity occupies much of the amniotic cavity   (MGI Ref ID J:37888)
  • enlarged heart
    • seen as early as E8.5   (MGI Ref ID J:37888)
  • growth/size/body phenotype
  • embryonic growth retardation
    • significantly delayed by E9.5 and are equivalent in size to normal E9 embryos   (MGI Ref ID J:37888)
  • nervous system phenotype
  • wavy neural tube
    • undulation of the neural tube   (MGI Ref ID J:37888)
  • muscle phenotype
  • abnormal myocardial fiber morphology
    • cardiac myocytes tend to disaggregate   (MGI Ref ID J:37888)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cdh2tm1Hyn related

Cardiovascular Research
Heart Abnormalities

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
Internal/Organ Defects
      heart

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cdh2tm1Hyn
Allele Name targeted mutation 1, Richard Hynes
Allele Type Targeted (Null/Knockout)
Common Name(s) Cdh2-; N-cadherin-;
Mutation Made ByDr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Cdh2, cadherin 2
Chromosome 18
Gene Common Name(s) CD325; CDHN; CDw325; N-cadherin; NCAD; Ncad; cadherin, N-;
Molecular Note A neomycin cassette was inserted into the exon 10, which encodes part of the extracellular domain of the protein. The protein was untectectable in Western blots of protein lysates derived from homozygous mutant embryos. [MGI Ref ID J:37888]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Radice GL; Rayburn H; Matsunami H; Knudsen KA; Takeichi M; Hynes RO. 1997. Developmental defects in mouse embryos lacking N-cadherin. Dev Biol 181(1):64-78. [PubMed: 9015265]  [MGI Ref ID J:37888]

Additional References

Cdh2tm1Hyn related

Broders-Bondon F; Paul-Gilloteaux P; Carlier C; Radice GL; Dufour S. 2012. N-cadherin and beta1-integrins cooperate during the development of the enteric nervous system. Dev Biol 364(2):178-91. [PubMed: 22342243]  [MGI Ref ID J:183947]

Edsbagge J; Johansson JK; Esni F; Luo Y; Radice GL; Semb H. 2005. Vascular function and sphingosine-1-phosphate regulate development of the dorsal pancreatic mesenchyme. Development 132(5):1085-92. [PubMed: 15689381]  [MGI Ref ID J:97177]

Esni F; Johansson BR; Radice GL; Semb H. 2001. Dorsal pancreas agenesis in N-cadherin- deficient mice. Dev Biol 238(1):202-12. [PubMed: 11784004]  [MGI Ref ID J:72022]

Horikawa K; Radice G; Takeichi M; Chisaka O. 1999. Adhesive subdivisions intrinsic to the epithelial somites. Dev Biol 215(2):182-9. [PubMed: 10545229]  [MGI Ref ID J:58409]

Kostetskii I; Li J; Xiong Y; Zhou R; Ferrari VA; Patel VV; Molkentin JD; Radice GL. 2005. Induced deletion of the N-cadherin gene in the heart leads to dissolution of the intercalated disc structure. Circ Res 96(3):346-54. [PubMed: 15662031]  [MGI Ref ID J:98828]

Lai CF; Cheng SL; Mbalaviele G; Donsante C; Watkins M; Radice GL; Civitelli R. 2006. Accentuated ovariectomy-induced bone loss and altered osteogenesis in heterozygous N-cadherin null mice. J Bone Miner Res 21(12):1897-906. [PubMed: 17002573]  [MGI Ref ID J:128076]

Luccardini C; Hennekinne L; Viou L; Yanagida M; Murakami F; Kessaris N; Ma X; Adelstein RS; Mege RM; Metin C. 2013. N-cadherin sustains motility and polarity of future cortical interneurons during tangential migration. J Neurosci 33(46):18149-60. [PubMed: 24227724]  [MGI Ref ID J:204170]

Luo Y; Ferreira-Cornwell M; Baldwin H; Kostetskii I; Lenox J; Lieberman M; Radice G. 2001. Rescuing the N-cadherin knockout by cardiac-specific expression of N- or E-cadherin. Development 128(4):459-69. [PubMed: 11171330]  [MGI Ref ID J:67158]

Luo Y; High FA; Epstein JA; Radice GL. 2006. N-cadherin is required for neural crest remodeling of the cardiac outflow tract. Dev Biol 299(2):517-28. [PubMed: 17014840]  [MGI Ref ID J:115264]

Luo Y; Radice GL. 2003. Cadherin-mediated adhesion is essential for myofibril continuity across the plasma membrane but not for assembly of the contractile apparatus. J Cell Sci 116(Pt 8):1471-9. [PubMed: 12640032]  [MGI Ref ID J:118482]

Plageman TF Jr; Zacharias AL; Gage PJ; Lang RA. 2011. Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis. Dev Biol 357(1):227-34. [PubMed: 21726547]  [MGI Ref ID J:175443]

Pontoriero GF; Smith AN; Miller LA; Radice GL; West-Mays JA; Lang RA. 2009. Co-operative roles for E-cadherin and N-cadherin during lens vesicle separation and lens epithelial cell survival. Dev Biol 326(2):403-17. [PubMed: 18996109]  [MGI Ref ID J:145191]

Smith AN; Miller LA; Radice G; Ashery-Padan R; Lang RA. 2009. Stage-dependent modes of Pax6-Sox2 epistasis regulate lens development and eye morphogenesis. Development 136(17):2977-85. [PubMed: 19666824]  [MGI Ref ID J:158133]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe strain exists on a mixed B6,129,CD1 background and can be maintained by heterozygous matings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)