Strain Name:

B10.Cg-Tg(Igh2k3-83)1Nemz/J

Stock Number:

003183

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B10.D2-Tg(Igh2k3-83)1Nemz/J    (Changed: 30-OCT-09 )
B10.D2-TgN(Igh2Kk3-83)1Nemz    (Changed: 15-DEC-04 )
B10.D2-TgN(Igh2Kk3-83    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Transgenic;
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. David Nemazee,   The Scripps Research Institute

Appearance
black
Related Genotype: a/a

Description
Hemizygous mice carry the immunoglobulin transgene Tg(Igh2k3-83)1Nemz. Results indicate that B cells specific for major histocompatibility complex class I antigen can be deleted if they encounter membrane-bound antigen at a post-bone-marrow stage of development. This deletion may be necessary to prevent organ-specific autoimmunity. The 3-83 transgenic only produce B-lymphocytes that make a single type of antibody with a defined specificity whose presence or absence can be easily monitored with several different reagents. Thus, they are invaluable to investigators who wish to determine how the development of a specific antibody specificity is genetically controlled. This is an important question in determining both how immune responses are generated against infectious agents, while normally preventing the development of antibodies against self-proteins which can lead to serious autoimmune diseases such as lupus. The 3-83 mice can also be be used to dissect the antibody production role of B-lymphocytes from their antigen presenting cell functions.

Development
Transgenic constructs containing rearranged functional immunoglobulin Igk-V, -J, -C and Igh-V,-D,-J,-6 gene segments from the BALB/c hybridoma 3-83 were injected into DBA/2 x B10D2F2 oocytes in the lab of Dr. David Nemazee at The Scripps Research Institute. The founder mouse was bred to B10.D2/nSnJ mice for an unknown number of generations before arriving at The Jackson Laboratory in 1997, where it was backcrossed to C57BL/10SnJ (Stock No. 000666) five times before embryos were cryopreserved and live mice removed from the shelf. The donor lab reports that approximately 4 copies of each construct cointegrated at the same locus.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Igh2k3-83)1Nemz allele
003172   NOD.D2(B10)-Tg(Igh2k3-83)1Nemz/Dvs
View Strains carrying   Tg(Igh2k3-83)1Nemz     (1 strain)

Phenotype

Phenotype Information

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Igh2k3-83)1Nemz
Allele Name transgene insertion 3-83, David Nemazee
Allele Type Transgenic (random, expressed)
Common Name(s) 3-83; 3-83mudelta; 3-83mudelta-TG; mudelta TG;
Strain of OriginDBA/2 X (C57BL/10 x DBA/2)F2
Expressed Gene Igh-Ia, immunoglobulin heavy chain Ia, mouse, laboratory
Promoter Igh-Ia, immunoglobulin heavy chain Ia, mouse, laboratory
Molecular Note This immunoglobulin transgene (3-83 mu delta, anti-K-k,K-b) encodes antibody reactive with the membrane-bound H-2K(b) antigen. [MGI Ref ID J:116735] [MGI Ref ID J:76119]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Igh2k3-83)1Nemz/J, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Russell DM; Dembic Z; Morahan G; Miller JF; Burki K; Nemazee D. 1991. Peripheral deletion of self-reactive B cells. Nature 354(6351):308-11. [PubMed: 1956380]  [MGI Ref ID J:76119]

Additional References

Kench JA; Russell DM; Nemazee D. 1998. Efficient peripheral clonal elimination of B lymphocytes in MRL/lpr mice bearing autoantibody transgenes. J Exp Med 188(5):909-17. [PubMed: 9730892]  [MGI Ref ID J:50289]

Tg(Igh2k3-83)1Nemz related

Carrasco YR; Batista FD. 2006. B-cell activation by membrane-bound antigens is facilitated by the interaction of VLA-4 with VCAM-1. EMBO J 25(4):889-99. [PubMed: 16456548]  [MGI Ref ID J:106020]

Caucheteux SM; Gendron MC; Kanellopoulos-Langevin C. 2005. Pregnancy-induced alterations of B cell maturation and survival are differentially affected by Fas and Bcl-2, independently of BcR expression. Int Immunol 17(1):55-63. [PubMed: 15569774]  [MGI Ref ID J:94796]

Caucheteux SM; Vernochet C; Wantyghem J; Gendron MC; Kanellopoulos-Langevin C. 2008. Tolerance induction to self-MHC antigens in fetal and neonatal mouse B cells. Int Immunol 20(1):11-20. [PubMed: 18032373]  [MGI Ref ID J:130195]

Depoil D; Fleire S; Treanor BL; Weber M; Harwood NE; Marchbank KL; Tybulewicz VL; Batista FD. 2008. CD19 is essential for B cell activation by promoting B cell receptor-antigen microcluster formation in response to membrane-bound ligand. Nat Immunol 9(1):63-72. [PubMed: 18059271]  [MGI Ref ID J:130478]

Edry E; Azulay-Debby H; Melamed D. 2008. TOLL-like receptor ligands stimulate aberrant class switch recombination in early B cell precursors. Int Immunol 20(12):1575-85. [PubMed: 18974086]  [MGI Ref ID J:143931]

Fokko van Loo P; Dingjan GM; Maas A; Hendriks RW. 2007. Surrogate-light-chain silencing is not critical for the limitation of pre-B cell expansion but is for the termination of constitutive signaling. Immunity 27(3):468-80. [PubMed: 17869135]  [MGI Ref ID J:125321]

Kench JA; Russell DM; Nemazee D. 1998. Efficient peripheral clonal elimination of B lymphocytes in MRL/lpr mice bearing autoantibody transgenes. J Exp Med 188(5):909-17. [PubMed: 9730892]  [MGI Ref ID J:50289]

Keren Z; Averbuch D; Shahaf G; Zisman-Rozen S; Golan K; Itkin T; Lapidot T; Mehr R; Melamed D. 2011. Chronic B cell deficiency from birth prevents age-related alterations in the B lineage. J Immunol 187(5):2140-7. [PubMed: 21810615]  [MGI Ref ID J:179123]

Kersseboom R; Kil L; Flierman R; van der Zee M; Dingjan GM; Middendorp S; Maas A; Hendriks RW. 2010. Constitutive activation of Bruton's tyrosine kinase induces the formation of autoreactive IgM plasma cells. Eur J Immunol 40(9):2643-54. [PubMed: 20623551]  [MGI Ref ID J:165753]

Lamoureux JL; Watson LC; Cherrier M; Skog P; Nemazee D; Feeney AJ. 2007. Reduced receptor editing in lupus-prone MRL/lpr mice. J Exp Med 204(12):2853-64. [PubMed: 17967905]  [MGI Ref ID J:128433]

Llorian M; Stamataki Z; Hill S; Turner M; Martensson IL. 2007. The PI3K p110delta is required for down-regulation of RAG expression in immature B cells. J Immunol 178(4):1981-5. [PubMed: 17277100]  [MGI Ref ID J:144002]

Maddox DM; Vessey KA; Yarbrough GL; Invergo BM; Cantrell DR; Inayat S; Balannik V; Hicks WL; Hawes NL; Byers S; Smith RS; Hurd R; Howell D; Gregg RG; Chang B; Naggert JK; Troy JB; Pinto LH; Nishina PM; McCall MA. 2008. Allelic variance between GRM6 mutants, Grm6nob3 and Grm6nob4 results in differences in retinal ganglion cell visual responses. J Physiol 586(Pt 18):4409-24. [PubMed: 18687716]  [MGI Ref ID J:152828]

Meade J; Tybulewicz VL; Turner M. 2004. The tyrosine kinase Syk is required for light chain isotype exclusion but dispensable for the negative selection of B cells. Eur J Immunol 34(4):1102-10. [PubMed: 15048721]  [MGI Ref ID J:131486]

Melamed D; Kench JA; Grabstein K; Rolink A; Nemazee D. 1997. A functional B cell receptor transgene allows efficient IL-7-independent maturation of B cell precursors. J Immunol 159(3):1233-9. [PubMed: 9233618]  [MGI Ref ID J:107402]

Nemazee DA; Burki K. 1989. Clonal deletion of B lymphocytes in a transgenic mouse bearing anti-MHC class I antibody genes. Nature 337(6207):562-6. [PubMed: 2783762]  [MGI Ref ID J:116735]

Rubio CF; Kench J; Russell DM; Yawger R; Nemazee D. 1996. Analysis of central B cell tolerance in autoimmune-prone MRL/lpr mice bearing autoantibody transgenes. J Immunol 157(1):65-71. [PubMed: 8683157]  [MGI Ref ID J:107233]

Saez de Guinoa J; Barrio L; Mellado M; Carrasco YR. 2011. CXCL13/CXCR5 signaling enhances BCR-triggered B-cell activation by shaping cell dynamics. Blood 118(6):1560-9. [PubMed: 21659539]  [MGI Ref ID J:176933]

Shivtiel S; Leider N; Melamed D. 2002. Receptor editing in CD45-deficient immature B cells. Eur J Immunol 32(8):2264-73. [PubMed: 12209639]  [MGI Ref ID J:136398]

Ta VB; de Haan AB; de Bruijn MJ; Dingjan GM; Hendriks RW. 2011. Pre-B-cell leukemias in Btk/Slp65-deficient mice arise independently of ongoing V(D)J recombination activity. Leukemia 25(1):48-56. [PubMed: 21030983]  [MGI Ref ID J:167638]

Verkoczy L; Ait-Azzouzene D; Skog P; Martensson A; Lang J; Duong B; Nemazee D. 2005. A role for nuclear factor kappa b/rel transcription factors in the regulation of the recombinase activator genes. Immunity 22(4):519-31. [PubMed: 15845455]  [MGI Ref ID J:97978]

Verkoczy L; Duong B; Skog P; Ait-Azzouzene D; Puri K; Vela JL; Nemazee D. 2007. Basal B cell receptor-directed phosphatidylinositol 3-kinase signaling turns off RAGs and promotes B cell-positive selection. J Immunol 178(10):6332-41. [PubMed: 17475862]  [MGI Ref ID J:146117]

Watson LC; Moffatt-Blue CS; McDonald RZ; Kompfner E; Ait-Azzouzene D; Nemazee D; Theofilopoulos AN; Kono DH; Feeney AJ. 2006. Paucity of V-D-D-J rearrangements and VH replacement events in lupus prone and nonautoimmune TdT-/- and TdT+/+ mice. J Immunol 177(2):1120-8. [PubMed: 16818769]  [MGI Ref ID J:134996]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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