Strain Name:

B6.Cg-Myo7ash1-8J/J

Stock Number:

003184

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain B6;SJL-TgN(c17lacZ)226Bri

Appearance
black, tremors
Related Genotype: a/a Myo7ash1-8J/Myo7ash1-8J

black, unaffected
Related Genotype: a/a Myo7ash1-8J/+

Description
Myo7ash1-8J homozygous mice circle and bob their heads; heterozygotes are behaviorally normal. Homozygotes of both sexes are viable and fertile (Samples 2000). The hearing of Myo7ash1-8J mutants has not been examined. However, other Myo7a mutations, whose behavioral phenotypes are virtually identical to that of Myo7ash1-J, are associated with deafness.

Mutations in the human ortholog of Myo7a, MYO7A, have been characterized in pedigrees of patients having Usher syndrome type 1 (USH1), an autosomal recessive disorder characterized by profound deafness, vestibular dysfunction and progressive retinitis pigmentosa (Weil et al., 1995; Weston et al., 1996; Adato et al., 1997; Levy et al., 1997; Liu et al., 1998; Espinos et al., 1998; Cuevas et al., 1998, 1999; Janecke et al., 1999). MYO7A mutations have also been identified in patients with autosomal dominant non-syndromic deafness (Liu et al., 1997) and autosomal recessive isolated deafness (Liu et al., 1997; Weil et al., 1997).

Development
The Myo7ash1-8J mutation occurred spontaneously on B6,SJL-TgN(c177lacZ)226Bri while the latter was being bred in the Importation facility at The Jackson Laboratory. Myo7ash1-8J was transferred to the C57BL/6J background by seven rounds of backcross-intercross breeding; homozygous males produced by mating heterozygous sibs were bred to C57BL/6J females to yield the next generation of heterozygotes. The colony was genotyped for TgN(c177lacZ)226Bri in March, 2000 and verified free of the transgene.

Control Information

  Control
   Heterozygote from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myo7a
005468   C57BL/6J-Myo7ash1-11J/J
000619   FS/EiJ
000271   SH1/LeJ
002919   STOCK Myo7ash1-7J/J
View Strains carrying other alleles of Myo7a     (4 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Deafness, Autosomal Dominant 11; DFNA11   (MYO7A)
Deafness, Autosomal Recessive 2; DFNB2   (MYO7A)
Usher Syndrome, Type I; USH1   (MYO7A)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Myo7ash1-8J/Myo7a+

        B6.Cg-Myo7ash1-8J/J
  • hearing/vestibular/ear phenotype
  • impaired hearing
    • slight but significant impairment compared to C57BL/6J controls only in older mice   (MGI Ref ID J:183898)

Myo7ash1-8J/Myo7ash1-8J

        B6.Cg-Myo7ash1-8J/J
  • hearing/vestibular/ear phenotype
  • abnormal cochlear hair cell stereociliary bundle morphology
    • hair bundles are disorganized at P2 and P6   (MGI Ref ID J:183898)
  • abnormal utricle morphology
    • hair bundles are disorganized at P2 and P6   (MGI Ref ID J:183898)
  • abnormal vestibular saccule morphology
    • hair bundles are disorganized at P2 and P6   (MGI Ref ID J:183898)
  • increased or absent threshold for auditory brainstem response
    • lack ABR response even at the highest stimulus intensity (100 dB sound pressure level)   (MGI Ref ID J:183898)
  • behavior/neurological phenotype
  • circling   (MGI Ref ID J:183898)
  • pigmentation phenotype
  • *normal* pigmentation phenotype
    • mice exhibit wild-type iris pigmentation   (MGI Ref ID J:141035)
  • nervous system phenotype
  • abnormal cochlear hair cell stereociliary bundle morphology
    • hair bundles are disorganized at P2 and P6   (MGI Ref ID J:183898)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Myo7ash1-8J/Myo7ash1-8J

        involves: C57BL/6J * SJL
  • behavior/neurological phenotype
  • abnormal parental behavior
    • homozygotes of both sexes breed, but are not good parents   (MGI Ref ID J:77937)
  • circling
    • mice walk in a circling motion   (MGI Ref ID J:77937)
  • head bobbing   (MGI Ref ID J:77937)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Myo7ash1-8J related

Neurobiology Research
Hearing Defects
Tremor Defects
Vestibular Defects

Sensorineural Research
Hearing Defects
Vestibular Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Myo7ash1-8J
Allele Name shaker 1, 8 Jackson
Allele Type Spontaneous
Strain of OriginB6;SJL-Tg(c177-lacZ)226Bri/J
Gene Symbol and Name Myo7a, myosin VIIA
Chromosome 7
Gene Common Name(s) DFNA11; DFNB2; Hdb; MYOVIIA; MYU7A; Myo7; NSRD2; USH1B; headbanger; myosin VII; neuroscience mutagenesis facility, 371; nmf371; polka; sh-1; sh1; shaker 1;
General Note

Genetic Background:

The 266Bri stock on which Myo7ash1-8J arose was homozygous for a lacZ transgene. The mutation was not the result of the transgene integration, as not all animals exhibited the neurological phenotype.
Molecular Note Two genomic regions encompassing a total of about 6.4 kb was deleted n this mutation. One region spans about 3.6 kb and covers exons 38 through 40. The other region spans about 2.8 kb and covers exons 46 and 47. Immunoblot analysis failed to detect protein in extracts from homozygous mice. [MGI Ref ID J:183898] [MGI Ref ID J:77937]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Gibson F; Walsh J; Mburu P; Varela A; Brown KA; Antonio M; Beisel KW; Steel KP; Brown SD. 1995. A type VII myosin encoded by the mouse deafness gene shaker-1. Nature 374(6517):62-4. [PubMed: 7870172]  [MGI Ref ID J:23257]

Weil D; Blanchard S; Kaplan J; Guilford P; Gibson F; Walsh J; Mburu P; Varela A; Levilliers J; Weston MD; Kelley PM; Kimberling WJ; Wagenaar M; Levi-Acobas F; Larget-Piet D; Munnich A; Steel KP; Brown SDM; Petit C.. 1995. Defective myosin VIIA gene responsible for Usher syndrome type 1B. Nature 374(6517):60-1. [PubMed: 7870171]  [MGI Ref ID J:23239]

Weil D; Kussel P; Blanchard S; Levy G; Levi-Acobas F; Drira M ; Ayadi H ; Petit C. 1997. The autosomal recessive isolated deafness, DFNB2, and the Usher 1B syndrome are allelic defects of the myosin-VIIA gene. Nat Genet 16(2):191-3. [PubMed: 9171833]  [MGI Ref ID J:40770]

Myo7ash1-8J related

Anderson MG; Hawes NL; Trantow CM; Chang B; John SW. 2008. Iris phenotypes and pigment dispersion caused by genes influencing pigmentation. Pigment Cell Melanoma Res 21(5):565-78. [PubMed: 18715234]  [MGI Ref ID J:141035]

Osterweil E; Wells DG; Mooseker MS. 2005. A role for myosin VI in postsynaptic structure and glutamate receptor endocytosis. J Cell Biol 168(2):329-38. [PubMed: 15657400]  [MGI Ref ID J:95877]

Samples RM; Ward-Bailey P; Washburn LL; Donahue LR. 2002. A new shaker 1 mutation of the Myo7 gene in the mouse MGI Direct Data Submission :.  [MGI Ref ID J:77937]

Zheng QY; Scarborough JD; Zheng Y; Yu H; Choi D; Gillespie PG. 2012. Digenic inheritance of deafness caused by 8J allele of myosin-VIIA and mutations in other Usher I genes. Hum Mol Genet 21(11):2588-98. [PubMed: 22381527]  [MGI Ref ID J:183898]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   Heterozygote from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)