Strain Name:

C57BL/6J-Tg(WapTAg)1Knw/J

Stock Number:

003188

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Common Names: WAPT1;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6J-Tg(WapTAg)1Knw    (Changed: 23-FEB-11 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Barbara Knowles,   Institute of Medical Biology

Description
Multiparous female mice of the WapTag1 lineage develop mammary adenocarcinomas with an average latency of 13 months. The histopathological phenotype is heterogeneous; papillary, ductal, tubular, and sometimes solid phenotypes are observed. Those tumors with a papillary morphology closely resemble human papillary carcinomas. Tumors arise adjacent to morphologically normal mammary epithelium or hyperplastic lesions. Mammary carcinomas are extremely rare in virgin female mice. Male mice, and those female mice that do not develop mammary carcinoma develop undifferentiated soft tissue sarcomas. Multiparous females of the WapTag3 lineage have hyperplastic mammary glands and occasionally develop mammary adenocarcinomas by 6 months of age. At this age, both males and females of this lineage develop osteosarcomas arising from the os petrosum and renal adenocarcinomas.

Development
A 1,600bp mouse whey acidic protein promoter (BglII-KpnI) driving the SV40 early region coding sequences (BglII-BamHI) was microinjected into the male pronucleus of C57BL/6J zygotes. This transgene has been maintained on the C57BL/6J background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of TAg     (16 strains)

View Strains carrying other alleles of Wap     (12 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(WapTAg)1Knw/0

        C57BL/6J-Tg(WapTAg)1Knw/J
  • tumorigenesis
  • increased mammary adenocarcinoma incidence
    • mammary adenocarcinoma is found in multiparous females but not males or virgin females, with an average latency of 13 months   (MGI Ref ID J:53175)
  • increased sarcoma incidence
    • in males and virgin females, but not multiparous females, poorly differentiated soft cell sarcomas are observed frequently, usually on the trunk or extremities, with average latency of 13 months   (MGI Ref ID J:53175)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors
      Mammary Gland Tumors: females only

TAg related

Apoptosis Research
Extracellular Modulators

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(WapTAg)1Knw
Allele Name transgene insertion 1, Barbara B Knowles
Allele Type Transgenic (random, expressed)
Common Name(s) WAPTag 1;
Strain of OriginC57BL/6J
Expressed Gene TAg, SV40 large T-antigen, SV40
Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter Wap, whey acidic protein, mouse, laboratory
Molecular Note The transgene contains the mouse Wap (whey acidic protein) promoter and the wild-type allele of the SV40 large T antigen (Tag) gene. [MGI Ref ID J:53175]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

TAg, SV, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Husler MR; Kotopoulis KA; Sundberg JP; Tennent BJ; Kunig SV; Knowles BB. 1998. Lactation-induced WAP-SV40 Tag transgene expression in C57BL/6J mice leads to mammary carcinoma. Transgenic Res 7(4):253-63. [PubMed: 9859214]  [MGI Ref ID J:53175]

Additional References

Tg(WapTAg)1Knw related

Cardiff RD; Anver MR; Gusterson BA; Hennighausen L; Jensen RA; Merino MJ; Rehm S; Russo J; Tavassoli FA; Wakefield LM; Ward JM; Green JE. 2000. The mammary pathology of genetically engineered mice: the consensus report and recommendations from the Annapolis meeting [see comments] Oncogene 19(8):968-88. [PubMed: 10713680]  [MGI Ref ID J:61035]

Herschkowitz JI; Simin K; Weigman VJ; Mikaelian I; Usary J; Hu Z; Rasmussen KE; Jones LP; Assefnia S; Chandrasekharan S; Backlund MG; Yin Y; Khramtsov AI; Bastein R; Quackenbush J; Glazer RI; Brown PH; Green JE; Kopelovich L; Furth PA; Palazzo JP; Olopade OI; Bernard PS; Churchill GA; Van Dyke T; Perou CM. 2007. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol 8(5):R76. [PubMed: 17493263]  [MGI Ref ID J:160877]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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