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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names WAPT1 (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Barbara Knowles, The Jackson Laboratory Description
Multiparous female mice of the WapTag1 lineage develop mammary adenocarcinomas with an average latency of 13 months. The histopathological phenotype is heterogeneous; papillary, ductal, tubular, and sometimes solid phenotypes are observed. Those tumors with a papillary morphology closely resemble human papillary carcinomas. Tumors arise adjacent to morphologically normal mammary epithelium or hyperplastic lesions. Mammary carcinomas are extremely rare in virgin female mice. Male mice, and those female mice that do not develop mammary carcinoma develop undifferentiated soft tissue sarcomas. Multiparous females of the WapTag3 lineage have hyperplastic mammary glands and occasionally develop mammary adenocarcinomas by 6 months of age. At this age, both males and females of this lineage develop osteosarcomas arising from the os petrosum and renal adenocarcinomas.Development
A 1,600bp mouse whey acidic protein promoter (BglII-KpnI) driving the SV40 early region coding sequences (BglII-BamHI) was microinjected into the male pronucleus of C57BL/6J zygotes. This transgene has been maintained on the C57BL/6J background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of TAg
008215 (C57BL/6-Tg(TRAMP)8247Ng/J X FVB/NJ)F1/J 003382 B10.D2-Tg(C3-1-TAg)cJeg/J 008247 B6.Cg-Tg(Ela1-TAg*)289Mjt/J 003380 B6.FVB-Tg(C3-1-TAg)cJeg/J 003135 C57BL/6-Tg(TRAMP)8247Ng/J 003445 C57BL/6J-Tg(Amy1TAg)354Knw/J 003446 C57BL/6J-Tg(Amy1TAg)501Knw/J 003477 C57BL/6J-Tg(SV)419Bri/J 003476 C57BL/6J-Tg(SV)427Bri/J 002233 C57BL/6J-Tg(SV)7Bri/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 005564 FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ 003381 FVB-Tg(C3-1-TAg)cJeg/J 002380 NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ 002033 NOD/ShiLt-Tg(RipTAg)1Lt/J View Strains carrying other alleles of TAg (15 strains)
003552 B6129-Tg(Wap-cre)11738Mam/J 002409 B6;SJL-Tg(Wap-HRAS)69Lln Chr YSJL/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 002500 C57BL/6J-Tg(WapIgf1)39Dlr/J 002410 FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL/J 003078 FVB.Cg-Tg(WapIgf1)39Dlr/J 002678 FVB.Cg-Tg(WapTgfa)215Bri/J 002755 FVB/N-Tg(WapNotch4)10Rnc/J
Phenotype
Phenotype Information
assigned by genotype
Tg(WapTAg)1Knw/0
C57BL/6J-Tg(WapTAg)1Knw
- tumorigenesis
- mammary adenocarcinoma (MGI Ref ID J:53175)
- mammary adenocarcinoma is found in multiparous females but not males or virgin females, with an average latency of 13 months
- sarcoma (MGI Ref ID J:53175)
- in males and virgin females, but not multiparous females, poorly differentiated soft cell sarcomas are observed frequently, usually on the trunk or extremities, with average latency of 13 months
This mouse can be used to support research in many areas including:
TAg relatedCancer Research
Increased Tumor Incidence (Mammary Gland Tumors: females only)
Apoptosis Research
Extracellular Modulators
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(WapTAg)1Knw | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Barbara B Knowles | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | WAPTag 1; | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | TAg, SV40 large T-antigen, SV40 | ||
| Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer. | |||
| Promoter | Wap, whey acidic protein, mouse, laboratory | ||
| General Note |
Line 3 was also produced. Multiparous female transgenic mice develop mammary adenocarcinomas with an average latency of 13 months. The histopathological phenotype is heterogeneous; papillary, ductal, tubular, and sometimes solid phenotypes are observed. Tumors with a papillary morphology closely resemble human papillary carcinomas. Tumors arise adjacent to morphologically normal mammary epithelium or hyperplastic lesions. Mammary carcinomas are extremely rare in virgin female transgenic mice. Male transgenic mice, and those female transgenic mice that do not develop mammary carcinomas develop undifferentiated soft tissue sarcomas. | ||
| Molecular Note | The transgene contains the mouse Wap (whey acidic protein) promoter and the wild-type allele of the SV40 large T antigen (Tag) gene. [MGI Ref ID J:53175] | ||
Genotyping
Genotyping Information
Genotyping Protocols
TAg, SV, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Husler MR; Kotopoulis KA; Sundberg JP; Tennent BJ; Kunig SV; Knowles BB. 1998. Lactation-induced WAP-SV40 Tag transgene expression in C57BL/6J mice leads to mammary carcinoma. Transgenic Res 7(4):253-63. [PubMed: 9859214] [MGI Ref ID J:53175]
Additional References
Tg(WapTAg)1Knw relatedCardiff RD; Anver MR; Gusterson BA; Hennighausen L; Jensen RA; Merino MJ; Rehm S; Russo J; Tavassoli FA; Wakefield LM; Ward JM; Green JE. 2000. The mammary pathology of genetically engineered mice: the consensus report and recommendations from the Annapolis meeting [see comments] Oncogene 19(8):968-88. [PubMed: 10713680] [MGI Ref ID J:61035]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $89.30 Female or Male Homozygous for Tg(WapTAg)1Knw *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $178.60 Homozygous for Tg(WapTAg)1Knw x Homozygous for Tg(WapTAg)1Knw
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $116.10 Female or Male Homozygous for Tg(WapTAg)1Knw *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $232.20 Homozygous for Tg(WapTAg)1Knw x Homozygous for Tg(WapTAg)1Knw
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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| fax: | 207-288-6655 |
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