Strain Name: |
B6.129S2-Pomctm1Low/J |
|---|---|
Stock Number: |
003191 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
B6.129S2-Pomc1tm1Low/J (Changed: 07-MAY-08
) |
|
B6.129S2-Pomctm1Low (Changed: 15-DEC-04
) | |
| Genes & Alleles | Pomc; Pomctm1Low; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S2 via D3 ES cell line Donating Investigator Malcolm Low, Oregon Health Sciences University Generation N12 (29-JAN-06) Appearance
black
Related Genotype: a/aStrain Description
Mice homozygous for the Pomctm1Low targeted mutation are viable and fertile. Homozygous mutant mice display no overt developmental or behavioral abnormalities. The hypothalmic-pituitary-adrenal axis functions normally. Homozygotes do display significantly greater nonopioid analgesia in response to cold water swim stress compared with controls and display paradoxical naloxone-induced analgesia. Male mice show an altered growth curve during puberty resulting in increased body mass and white fat. This phenotype is present in both the homozygous mutant males and in wildtype males reared by homozygous parents. This strain is useful for looking at response to pain.Strain Development
The gene targeting vector POMCX*4 encoding a truncated POMC prohormone caused by a point mutation in exon 3 and containing the phosphoglycerate kinase-neo and phosphoglycerate kinase-tk selection cassettes was constructed and electroporated into D3 embryonic stem cells. Correctly targeted ES cells were microinjected into C56BL/6 blastocysts.Per the investigator:
Gene & Allele Details
| Allele Symbol | Pomctm1Low | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Malcolm J Low | ||
| Common Name(s) | End-; POMCX*4-; betaend-; | ||
| Mutation Made By | Malcolm Low, Oregon Health Sciences University | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Pomc, pro-opiomelanocortin-alpha | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | ACTH; BE; CLIP; LPH; MSH; NPP; POC; Pomc-1; Pomc1; Pomc2; adrenal corticotropic hormone; alpha-MSH; alpha-melanocyte stimulating hormone; alphaMSH; beta-MSH; beta-endorphin; beta-melanocyte stimulating hormone; gamma-MSH; gamma-melanocyte stimulating hormone; | ||
| General Note | Additional reference J:65685 identifies the ES cell line origin as 129S2/SvEv. It is more likely that the mice used in this additional reference were derived from the targeted D3 ES cells (129S2/SvPas) first generated in J:78759 and implanted in mice in J:47711. | ||
| Molecular Note | A PGK-neomycin based targeting vector was used to introduce a premature start codon into exon 3, resulting in the truncation of the translated protein. In situ hybridization on homozygous mutant animals demonstrates that the truncation event had no effect on the distribution or level of transcript. Immunoreactivity demonstrated that Homozygous mutant mice had no detectable beta endorphin activity in the hypothalamus or pituitary [MGI Ref ID J:78759] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Pomctm1Low
Colony Maintenance
| Breeding & Husbandry | This strain may be maintained by mating homozygous siblings. Male offspring of homozygote beta-endorphin deficient parents have increased weight and white adipose tissue as adults compared to rearing by wildtype mothers. Expected coat color from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Pomc
008115 B6.129X1-Pomctm2Ute/J 006421 FVB-Tg(Pomc1-hrGFP)1Lowl/J 005965 STOCK Tg(Pomc1-cre)16Lowl/J View Strains carrying other alleles of Pomc (3 strains)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:Pomctm1Low related
Diabetes and Obesity Research
Obesity Without Diabetes
Mouse/Human Gene Homologs
obesity, adrenal insufficiency
Neurobiology Research
Behavioral and Learning Defects
References
Selected Reference(s)
Additional ReferencesRubinstein M; Mogil JS; Japon M; Chan EC; Allen RG; Low MJ. 1996. Absence of opioid stress-induced analgesia in mice lacking beta-endorphin by site-directed mutagenesis. Proc Natl Acad Sci U S A 93(9):3995-4000. [PubMed: 8633004] [MGI Ref ID J:47711]
Price and Supply Information
| Strain Name: | B6.129S2-Pomctm1Low/J |
| Stock Number: | 003191 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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