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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B10.PL-H2u H2-T18a (73NS)/Sn-TgN(TCRA)B1Jg (Changed: 15-DEC-04 ) B10.PL-H2u H2-T18a/(73NS)Sn-TgN(TCRA)B1Jg (Changed: 15-DEC-04 ) Type Congenic; Major Histocompatibility Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain B10.PL-H2u H2-T18a (73NS)/Sn Donor Strain B6D2F1 Generation +N1
Generation DefinitionsDonating Investigator Joan Goverman, University of Washington Appearance
black
Related Genotype: a/aDescription
TCR-transgenic mice exhibit the generation of an unusual population of CD4-CD8-TCR+ thymocytes and the absence of gamma delta cells. Transgenic TCR alpha chain causes thymocytes to differentiate into a CD4-CD8-TCR+ lineage at an early developmental stage, depleting the number of thymocytes that differentiate into the alpha-beta lineage. This transgene is also associated with the development of T cell lymphosarcoma. In combination with the transgenic strain TCR beta, it can be used as a model for Multiple Sclerosis.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000458 B10.PL-H2u H2-T18a/(73NS)SnJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of H2-T18
View Strains carrying other alleles of H2-T18 (32 strains)
Phenotype
Phenotype Information
assigned by genotype
H2u/H2u Tg(TCRA)B1Jg/0
B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J
- hematopoietic system phenotype
- abnormal thymus cell ratio
- the ratio of double negative to double-positive thymocytes is significantly higher (0.281) in these mice than in controls (0.016) (MGI Ref ID J:92992)
- decreased CD4-positive T cell number
- the absolute number of single-positive CD4 thymocytes is decreased (MGI Ref ID J:92992)
- decreased double-positive T cell number
- double-positive thymocyte numbers are decreased to 61.8% of all thymocytes compared to 75.3% for controls (MGI Ref ID J:92992)
- decreased thymocyte number
- thymus cellularity is reduced by 4 fold compared to controls (MGI Ref ID J:92992)
- increased double-negative T cell number
- immune system phenotype
- abnormal thymus cell ratio
- the ratio of double negative to double-positive thymocytes is significantly higher (0.281) in these mice than in controls (0.016) (MGI Ref ID J:92992)
- decreased CD4-positive T cell number
- the absolute number of single-positive CD4 thymocytes is decreased (MGI Ref ID J:92992)
- decreased double-positive T cell number
- double-positive thymocyte numbers are decreased to 61.8% of all thymocytes compared to 75.3% for controls (MGI Ref ID J:92992)
- decreased thymocyte number
- thymus cellularity is reduced by 4 fold compared to controls (MGI Ref ID J:92992)
- increased double-negative T cell number
- tumorigenesis
- T cell derived lymphoma
- cellular phenotype
- decreased double-positive T cell number
- double-positive thymocyte numbers are decreased to 61.8% of all thymocytes compared to 75.3% for controls (MGI Ref ID J:92992)
- decreased thymocyte number
- thymus cellularity is reduced by 4 fold compared to controls (MGI Ref ID J:92992)
- increased double-negative T cell number
This mouse can be used to support research in many areas including:
Research Tools
Immunology and Inflammation Research
T Cell Receptor Transgenics
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(TCRA)B1Jg | ||
|---|---|---|---|
| Allele Name | transgene insertion B1, Joan M Goverman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Joan Goverman, University of Washington | ||
| Strain of Origin | C57BL/6 x DBA/2 | ||
| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Molecular Note | A functionally rearranged Tcra gene was used in the construction of this transgene. A recombined Tcra gene with Valpha2.3 and JalphaTA39 gene segments was cloned from the B10.PL hybridoma "172.10". This hybridoma was specific for the 11 amino-terminal residues of myelin basic protein (MBP). Crossing this line with the Tg(TCRB)C14Jg line produces a T cell with a transgenic TCR that has high affinity for the first 11 amino acids of MBP. In these doubly transgenic mice, 50-95% of CD3+ cells expressed the transgenic Tcra gene. [MGI Ref ID J:92991] | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| Gene Symbol and Name | H2-T18, histocompatibility 2, T region locus 18 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2T18; TL Ag; Tla; thymus leukemia antigen; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TCRA)B1Jg, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Brabb T; Rubicz R; Mannikko V; Goverman J. 1997. Separately expressed T cell receptor alpha and beta chain transgenes exert opposite effects on T cell differentiation and neoplastic transformation. Eur J Immunol 27(11):3039-48. [PubMed: 9394835] [MGI Ref ID J:92992]
Additional References
Tg(TCRA)B1Jg relatedBrabb T; Goldrath AW; von Dassow P; Paez A; Liggitt HD; Goverman J. 1997. Triggers of autoimmune disease in a murine TCR-transgenic model for multiple sclerosis. J Immunol 159(1):497-507. [PubMed: 9200491] [MGI Ref ID J:92993]
Brabb T; von Dassow P; Ordonez N; Schnabel B; Duke B; Goverman J. 2000. In situ tolerance within the central nervous system as a mechanism for preventing autoimmunity. J Exp Med 192(6):871-80. [PubMed: 10993917] [MGI Ref ID J:107210]
Carrithers MD; Carrithers LM; Czyzyk J; Henegariu O. 2007. Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. J Neuroimmunol 187(1-2):31-43. [PubMed: 17512611] [MGI Ref ID J:128862]
Deng C; Minguela A; Hussain RZ; Lovett-Racke AE; Radu C; Ward ES; Racke MK. 2002. Expression of the tyrosine phosphatase SRC homology 2 domain-containing protein tyrosine phosphatase 1 determines T cell activation threshold and severity of experimental autoimmune encephalomyelitis. J Immunol 168(9):4511-8. [PubMed: 11970996] [MGI Ref ID J:125454]
Goverman J; Woods A; Larson L; Weiner LP; Hood L; Zaller DM. 1993. Transgenic mice that express a myelin basic protein-specific T cell receptor develop spontaneous autoimmunity. Cell 72(4):551-60. [PubMed: 7679952] [MGI Ref ID J:92991]
Huss DJ; Winger RC; Peng H; Yang Y; Racke MK; Lovett-Racke AE. 2010. TGF-beta enhances effector Th1 cell activation but promotes self-regulation via IL-10. J Immunol 184(10):5628-36. [PubMed: 20393141] [MGI Ref ID J:160999]
Li J; Vandal O; Sant'Angelo DB. 2011. TCR affinity for self-ligands influences the development and function of encephalitogenic T cells. PLoS One 6(3):e17702. [PubMed: 21437282] [MGI Ref ID J:171686]
Song F; Gienapp IE; Wang X; Whitacre CC. 2002. Activation of Vbeta8 T cells affects spontaneous EAE in MBP TCR transgenic mice. J Neuroimmunol 123(1-2):112-22. [PubMed: 11880156] [MGI Ref ID J:132051]
Yang Y; Weiner J; Liu Y; Smith AJ; Huss DJ; Winger R; Peng H; Cravens PD; Racke MK; Lovett-Racke AE. 2009. T-bet is essential for encephalitogenicity of both Th1 and Th17 cells. J Exp Med 206(7):1549-64. [PubMed: 19546248] [MGI Ref ID J:150271]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000458 B10.PL-H2u H2-T18a/(73NS)SnJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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