Description

Strain Information

Former Names B10.PL-H2u H2-T18a (73NS)/Sn-TgN(TCRA)B1Jg    (Changed: 15-DEC-04 ) B10.PL-H2u H2-T18a/(73NS)Sn-TgN(TCRA)B1Jg    (Changed: 15-DEC-04 ) Type Congenic; Major Histocompatibility Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain B10.PL-H2u H2-T18a (73NS)/Sn Donor Strain B6D2F1 Generation +N1   Donating Investigator Joan Goverman,   University of Washington

Appearance
black
Related Genotype: a/a

Description
TCR-transgenic mice exhibit the generation of an unusual population of CD4^-CD8^-TCR⁺ thymocytes and the absence of gamma delta cells. Transgenic TCR alpha chain causes thymocytes to differentiate into a CD4^-CD8^-TCR⁺ lineage at an early developmental stage, depleting the number of thymocytes that differentiate into the alpha-beta lineage. This transgene is also associated with the development of T cell lymphosarcoma. In combination with the transgenic strain TCR beta, it can be used as a model for Multiple Sclerosis.

Control Information

	Control
	Wild-type from the colony
	000458 B10.PL-H2u H2-T18a/(73NS)SnJ
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Tcra related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Research Tools
Cancer Research (specific T cell deficiency)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(TCRA)B1Jg
Allele Name		transgene insertion B1, Joan M Goverman
Allele Type		Transgenic (random, expressed)
Mutation Made By		Joan Goverman,   University of Washington
Strain of Origin		C57BL/6 x DBA/2
Expressed Gene		Tcra, T-cell receptor alpha chain, mouse, laboratory
Promoter		Tcra, T-cell receptor alpha chain, mouse, laboratory
General Note		Thymocytes in transgenic mice differentiate into a CD4-CD8-TCR+ lineage at an early developmental stage, depleting the number of thymocytes that differentiate into the alpha-beta lineage. Gamma delta cells are absent in these mice. The transgene is also associated with the development of T cell lymphosarcoma. Crossing this line with the Tg(TCRB)C14Jg line produces a T cell with a transgenic TCR that has affinity for the first 11 amino acids of myelin basic protein (MBP).
Molecular Note		The transgene contains the T-cell receptor alpha chain gene. [MGI Ref ID J:92991]
Gene Symbol and Name		H2-T18, histocompatibility 2, T region locus 18
Chromosome		17
Gene Common Name(s)		H-2T18; TL Ag; Tla; thymus leukemia antigen;
Gene Symbol and Name		H2, histocompatibility-2, MHC
Chromosome		17
Gene Common Name(s)		H-2; MHC-II;

Genotyping

Genotyping Information

Genotyping Protocols

Tg(TCRA)B1Jg, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Brabb T; Rubicz R; Mannikko V; Goverman J. 1997. Separately expressed T cell receptor alpha and beta chain transgenes exert opposite effects on T cell differentiation and neoplastic transformation. Eur J Immunol 27(11):3039-48. [PubMed: 9394835]  [MGI Ref ID J:92992]

Additional References

Tg(TCRA)B1Jg related

Brabb T; Goldrath AW; von Dassow P; Paez A; Liggitt HD; Goverman J. 1997. Triggers of autoimmune disease in a murine TCR-transgenic model for multiple sclerosis. J Immunol 159(1):497-507. [PubMed: 9200491]  [MGI Ref ID J:92993]

Brabb T; von Dassow P; Ordonez N; Schnabel B; Duke B; Goverman J. 2000. In situ tolerance within the central nervous system as a mechanism for preventing autoimmunity. J Exp Med 192(6):871-80. [PubMed: 10993917]  [MGI Ref ID J:107210]

Carrithers MD; Carrithers LM; Czyzyk J; Henegariu O. 2007. Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. J Neuroimmunol 187(1-2):31-43. [PubMed: 17512611]  [MGI Ref ID J:128862]

Deng C; Minguela A; Hussain RZ; Lovett-Racke AE; Radu C; Ward ES; Racke MK. 2002. Expression of the tyrosine phosphatase SRC homology 2 domain-containing protein tyrosine phosphatase 1 determines T cell activation threshold and severity of experimental autoimmune encephalomyelitis. J Immunol 168(9):4511-8. [PubMed: 11970996]  [MGI Ref ID J:125454]

Goverman J; Woods A; Larson L; Weiner LP; Hood L; Zaller DM. 1993. Transgenic mice that express a myelin basic protein-specific T cell receptor develop spontaneous autoimmunity. Cell 72(4):551-60. [PubMed: 7679952]  [MGI Ref ID J:92991]

Song F; Gienapp IE; Wang X; Whitacre CC. 2002. Activation of Vbeta8 T cells affects spontaneous EAE in MBP TCR transgenic mice. J Neuroimmunol 123(1-2):112-22. [PubMed: 11880156]  [MGI Ref ID J:132051]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	000458 B10.PL-H2u H2-T18a/(73NS)SnJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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