| |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B10.PL-H2u H2-T18a (73NS)/Sn-TgN(TCRB)C14Jg (Changed: 15-DEC-04 ) B10.PL-H2u H2-T18a/(73NS)Sn-TgN(TCRB)C14Jg (Changed: 15-DEC-04 ) Type Congenic; Major Histocompatibility Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain B10.PL-H2u H2-T18a (73NS)/Sn Donor Strain mix of C57BL/6 x DBA/2 Generation N16 Donating Investigator Joan Goverman, University of Washington Appearance
black
Related Genotype: a/aDescription
TCR-transgenic mice exhibit the generation of an unusual population of CD4-CD8-TCR+ thymocytes and the absence of gama delta cells. Transgenic TCR beta chain causes immature T cells to accelerate differentiation into the alpha-beta lineage and thus inhibits the generation of gamma-delta cells. In combination with the transgenic strain TCR alpha, it can be used as a model for Multiple Sclerosis.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000458 B10.PL-H2u H2-T18a/(73NS)SnJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of H2-T18
View Strains carrying other alleles of H2-T18 (33 strains)
Additional Web Information
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
H2u/H2u Tg(TCRB)C14Jg/0
B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J
- immune system phenotype
- abnormal double-negative T cell morphology (MGI Ref ID J:92992)
- a greater proportion of the double-negative thymocytes present in E16 and E17 thymi are in the most mature (c-kit-CD44-CD25-) stage compared to controls
- abnormal double-positive T cell morphology (MGI Ref ID J:92992)
- the appearance of double-positive T cells in the thymus first occurs on E15 compared to E17 for controls
- absent gamma-delta T cells (MGI Ref ID J:92992)
- gamma-delta T cells are not detected in the thymus of E18 mice
- hematopoietic system phenotype
- abnormal double-negative T cell morphology (MGI Ref ID J:92992)
- a greater proportion of the double-negative thymocytes present in E16 and E17 thymi are in the most mature (c-kit-CD44-CD25-) stage compared to controls
- abnormal double-positive T cell morphology (MGI Ref ID J:92992)
- the appearance of double-positive T cells in the thymus first occurs on E15 compared to E17 for controls
- absent gamma-delta T cells (MGI Ref ID J:92992)
- gamma-delta T cells are not detected in the thymus of E18 mice
This mouse can be used to support research in many areas including:
Tcrb relatedResearch Tools
Immunology and Inflammation Research
T Cell Receptor Transgenics
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TCRB)C14Jg | ||
|---|---|---|---|
| Allele Name | transgene insertion C14, Joan M Goverman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Joan Goverman, University of Washington | ||
| Strain of Origin | C57BL/6 x DBA/2 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Promoter | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Molecular Note | A functionally rearranged Tcrb gene was used in the construction of this transgene A recombined Tcrb gene with Vbeta8.2/Dbeta2/Jbeta2.6 gene segments was cloned from the B10.PL hybridoma "172.10". This hybridoma was specific for the 11 amino-terminal residues of myelin basic protein (MBP). Crossing this line with the Tg(TCRA)B1Jg line produces a T cell with a transgenic TCR that has affinity for the first 11 amino acids of MBP. In these doubly transgenic mice, almost all of the CD3+ cells express the transgenic Tcrb gene. [MGI Ref ID J:92991] | ||
| Gene Symbol and Name | H2-T18, histocompatibility 2, T region locus 18 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2T18; TL Ag; Tla; thymus leukemia antigen; | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TCRB)C14Jg, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Brabb T; Rubicz R; Mannikko V; Goverman J. 1997. Separately expressed T cell receptor alpha and beta chain transgenes exert opposite effects on T cell differentiation and neoplastic transformation. Eur J Immunol 27(11):3039-48. [PubMed: 9394835] [MGI Ref ID J:92992]
Additional References
Tg(TCRB)C14Jg relatedBrabb T; Goldrath AW; von Dassow P; Paez A; Liggitt HD; Goverman J. 1997. Triggers of autoimmune disease in a murine TCR-transgenic model for multiple sclerosis. J Immunol 159(1):497-507. [PubMed: 9200491] [MGI Ref ID J:92993]
Brabb T; von Dassow P; Ordonez N; Schnabel B; Duke B; Goverman J. 2000. In situ tolerance within the central nervous system as a mechanism for preventing autoimmunity. J Exp Med 192(6):871-80. [PubMed: 10993917] [MGI Ref ID J:107210]
Carrithers MD; Carrithers LM; Czyzyk J; Henegariu O. 2007. Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. J Neuroimmunol 187(1-2):31-43. [PubMed: 17512611] [MGI Ref ID J:128862]
Dao T; Guo D; Ploss A; Stolzer A; Saylor C; Boursalian TE; Im JS; Sant'Angelo DB. 2004. Development of CD1d-restricted NKT cells in the mouse thymus. Eur J Immunol 34(12):3542-52. [PubMed: 15549774] [MGI Ref ID J:94598]
Deng C; Minguela A; Hussain RZ; Lovett-Racke AE; Radu C; Ward ES; Racke MK. 2002. Expression of the tyrosine phosphatase SRC homology 2 domain-containing protein tyrosine phosphatase 1 determines T cell activation threshold and severity of experimental autoimmune encephalomyelitis. J Immunol 168(9):4511-8. [PubMed: 11970996] [MGI Ref ID J:125454]
Goverman J; Woods A; Larson L; Weiner LP; Hood L; Zaller DM. 1993. Transgenic mice that express a myelin basic protein-specific T cell receptor develop spontaneous autoimmunity. Cell 72(4):551-60. [PubMed: 7679952] [MGI Ref ID J:92991]
Song F; Gienapp IE; Wang X; Whitacre CC. 2002. Activation of Vbeta8 T cells affects spontaneous EAE in MBP TCR transgenic mice. J Neuroimmunol 123(1-2):112-22. [PubMed: 11880156] [MGI Ref ID J:132051]
Yang Y; Weiner J; Liu Y; Smith AJ; Huss DJ; Winger R; Peng H; Cravens PD; Racke MK; Lovett-Racke AE. 2009. T-bet is essential for encephalitogenicity of both Th1 and Th17 cells. J Exp Med 206(7):1549-64. [PubMed: 19546248] [MGI Ref ID J:150271]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000458 B10.PL-H2u H2-T18a/(73NS)SnJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.