Strain Name:

129S-Csktm1Sor/J

Stock Number:

003201

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129/Sv-Csktm1Sor/J    (Changed: 14-NOV-12 )
Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Philippe Soriano,   Mount Sinai School of Medicine

Description
Mice homozygous for disruption of the csk gene die in utero at 9 - 10 days of gestation, exhibiting defects in neurulation. Mutant embryos are anatomically indistinguishable from normal or heterozygous littermates at E8.5, but are identifiable at E9.5 by their smaller size, failure to 'turn' (reverse orientation of germ layers) and failure to close their cephalic neural folds. The allantois of these mutant embryos is also abnormal and does not connect with the chorion, preventing formation of the umbilical cord and placenta. The kinase Csk plays a role in negative regulation of the Src family tyrosine kinases by phosphorylating a key carboxy-terminal tyrosine residue. Expression of csk in normal embryos is detectable at low levels beginning at E8.5, and is at its highest level of expression at E9.5.

Development
The targeting vector included a neo expression cassette, which replaced all csk coding sequences flanked by 6kb of csk genomic sequences. Electroporation of the construct into AB1 embryonic cells and positive/negative selection with G418 and FIAU resulted in eight PCR-assayed positive clones. This is a null mutation.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Csktm1Sor allele
025250   B6.Cg-Csktm1Sor Tg(Csk*)2Weis/WeisJ
View Strains carrying   Csktm1Sor     (1 strain)

Strains carrying other alleles of Csk
018320   B6.129P2-Csktm1Tara/J
025250   B6.Cg-Csktm1Sor Tg(Csk*)2Weis/WeisJ
View Strains carrying other alleles of Csk     (2 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Csktm1Sor/Csk+

        129P2.129S7-Csktm1Sor
  • behavior/neurological phenotype
  • abnormal food preference
    • given a chose of two novel food sources they choose a food they have already seen another mouse eat   (MGI Ref ID J:188631)
  • abnormal object recognition memory
    • no increased investigation of displaced familiar objects, similar to 129P2 control but unlike C57BL/6 control   (MGI Ref ID J:188631)
  • abnormal social investigation
    • increased social approach of a strange mouse as opposed to investigating an empty cage or examining a pre-exposed familiar mouse   (MGI Ref ID J:188631)
    • less time is spent re-investigating a strange mouse during second exposure to that mouse   (MGI Ref ID J:188631)
    • given a chose of two novel food sources they choose a food they have already seen another mouse eat   (MGI Ref ID J:188631)
  • decreased anxiety-related response
    • increased time spent exploring open arms of an elevated + maze   (MGI Ref ID J:188631)

Csktm1Sor/Csktm1Sor

        either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6J)
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • most homozygotes die between E9.5 and E10.5   (MGI Ref ID J:12622)
    • no homozygotes are recovered at or after E11.5   (MGI Ref ID J:12622)
  • embryogenesis phenotype
  • abnormal allantois morphology
    • at E9.5, the allantois is often abnormal and either resorbing or tuberous   (MGI Ref ID J:12622)
    • enlarged allantois
      • allantois is often overgrown with a "bleb" at its extremity   (MGI Ref ID J:12622)
  • abnormal cephalic neural fold morphology
    • abnormal folding of the neuroectoderm is noted at the level of the cephalic folds and of the neural tube   (MGI Ref ID J:12622)
  • absent notochord
    • at E8.5, the mutant notochord is consistently smaller or even absent   (MGI Ref ID J:12622)
  • caudal body truncation
    • at E9.5, homozygotes exhibit a small and underdeveloped caudal portion   (MGI Ref ID J:12622)
  • decreased embryo size
    • at E9.5, most homozygous mutant embryos are ~25% to ~50% the size of wild-type embryos   (MGI Ref ID J:12622)
  • incomplete embryo turning
    • at E9.5, most homozygous mutant embryos remain unturned   (MGI Ref ID J:12622)
  • incomplete rostral neuropore closure
    • at E9.5, homozygotes fail to close their cephalic neural folds   (MGI Ref ID J:12622)
  • open neural tube
    • at E9.5, the mutant neural tube is closed at the level of the otic vesicle but remains open beyond the midbrain-forebrain boundary   (MGI Ref ID J:12622)
    • abnormal folding of the neuroectoderm is noted at the level of the cephalic folds and of the neural tube   (MGI Ref ID J:12622)
  • wavy neural tube
    • at E9.5, homozygotes exhibit a wavy and disorganized neural tube   (MGI Ref ID J:12622)
    • in addition, the neural tube is often curved and infiltrated with pyknotic cells   (MGI Ref ID J:12622)
  • nervous system phenotype
  • incomplete rostral neuropore closure
    • at E9.5, homozygotes fail to close their cephalic neural folds   (MGI Ref ID J:12622)
  • open neural tube
    • at E9.5, the mutant neural tube is closed at the level of the otic vesicle but remains open beyond the midbrain-forebrain boundary   (MGI Ref ID J:12622)
    • abnormal folding of the neuroectoderm is noted at the level of the cephalic folds and of the neural tube   (MGI Ref ID J:12622)
  • wavy neural tube
    • at E9.5, homozygotes exhibit a wavy and disorganized neural tube   (MGI Ref ID J:12622)
    • in addition, the neural tube is often curved and infiltrated with pyknotic cells   (MGI Ref ID J:12622)
  • growth/size/body phenotype
  • decreased embryo size
    • at E9.5, most homozygous mutant embryos are ~25% to ~50% the size of wild-type embryos   (MGI Ref ID J:12622)
  • megacephaly
    • at E9.5, homozygotes display a disproportionately bulky and dilated head   (MGI Ref ID J:12622)
  • cardiovascular system phenotype
  • poor circulation
    • at E9.5, blood circulation is essentially absent in the visceral yolk sac and in mutant embryos   (MGI Ref ID J:12622)
    • however, all homozygotes at this stage show normal decidual sizes and normal beating of the heart   (MGI Ref ID J:12622)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Csktm1Sor related

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Genes Regulating Growth and Proliferation

Developmental Biology Research
Embryonic Lethality (Homozygous)
Neural Tube Defects
Postnatal Lethality

Neurobiology Research
Neural Tube Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Csktm1Sor
Allele Name targeted mutation 1, Philippe Soriano
Allele Type Targeted (Null/Knockout)
Common Name(s) Csk-;
Mutation Made ByDr. Philippe Soriano,   Mount Sinai School of Medicine
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Csk, c-src tyrosine kinase
Chromosome 9
Gene Common Name(s) AW212630; expressed sequence AW212630;
Molecular Note Deletion of all coding sequences and replacement with a neomycin cassette. [MGI Ref ID J:12622]

Genotyping

Genotyping Information

Genotyping Protocols

Csktm1Sor,

Separated MCA


Csktm1Sor, Separated PCR
Csktm1Sor, Standard PCR
NEOTD (Generic Neo), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Imamoto A; Soriano P. 1993. Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice. Cell 73(6):1117-24. [PubMed: 7685657]  [MGI Ref ID J:12622]

Additional References

Csktm1Sor related

Duan LJ; Imamoto A; Fong GH. 2004. Dual roles of the C-terminal Src kinase (Csk) during developmental vascularization. Blood 103(4):1370-2. [PubMed: 14576066]  [MGI Ref ID J:115663]

Sinai L; Mathew R; Roder JC. 2012. Impaired social memories in 129P2 inbred mice are rescued by reduced Csk expression. Genes Brain Behav 11(5):559-67. [PubMed: 22348736]  [MGI Ref ID J:188631]

Tan YX; Manz BN; Freedman TS; Zhang C; Shokat KM; Weiss A. 2014. Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling. Nat Immunol 15(2):186-94. [PubMed: 24317039]  [MGI Ref ID J:208674]

Thomas SM; Soriano P; Imamoto A. 1995. Specific and redundant roles of Src and Fyn in organizing the cytoskeleton. Nature 376(6537):267-71. [PubMed: 7617039]  [MGI Ref ID J:113005]

Zikherman J; Jenne C; Watson S; Doan K; Raschke W; Goodnow CC; Weiss A. 2010. CD45-Csk phosphatase-kinase titration uncouples basal and inducible T cell receptor signaling during thymic development. Immunity 32(3):342-54. [PubMed: 20346773]  [MGI Ref ID J:158876]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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