Description

Strain Information

Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+1   Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center

Description
Mice homozygous for disruption of the csk gene die in utero at 9 - 10 days of gestation, exhibiting defects in neurulation. Mutant embryos are anatomically indistinguishable from normal or heterozygous littermates at E8.5, but are identifiable at E9.5 by their smaller size, failure to 'turn' (reverse orientation of germ layers) and failure to close their cephalic neural folds. The allantois of these mutant embryos is also abnormal and does not connect with the chorion, preventing formation of the umbilical cord and placenta. The kinase Csk plays a role in negative regulation of the Src family tyrosine kinases by phosphorylating a key carboxy-terminal tyrosine residue. Expression of csk in normal embryos is detectable at low levels beginning at E8.5, and is at its highest level of expression at E9.5.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Csk^tm1Sor/Csk^tm1Sor

        either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6J)

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:12622)
  • most homozygotes die between E9.5 and E10.5
  • no homozygotes are recovered at or after E11.5
• embryogenesis phenotype
• abnormal allantois morphology (MGI Ref ID J:12622)
  • at E9.5, the allantois is often abnormal and either resorbing or tuberous
• enlarged allantois (MGI Ref ID J:12622)
  • allantois is often overgrown with a "bleb" at its extremity
• absent notochord (MGI Ref ID J:12622)
  • at E8.5, the mutant notochord is consistently smaller or even absent
• caudal body truncation (MGI Ref ID J:12622)
  • at E9.5, homozygotes exhibit a small and underdeveloped caudal portion
• incomplete embryo turning (MGI Ref ID J:12622)
  • at E9.5, most homozygous mutant embryos remain unturned
• reduced embryo size (MGI Ref ID J:12622)
  • at E9.5, most homozygous mutant embryos are ~25% to ~50% the size of wild-type embryos
• nervous system phenotype
• incomplete cephalic closure (MGI Ref ID J:12622)
  • at E9.5, homozygotes fail to close their cephalic neural folds
• open neural tube (MGI Ref ID J:12622)
  • at E9.5, the mutant neural tube is closed at the level of the otic vesicle but remains open beyond the midbrain-forebrain boundary
  • abnormal folding of the neuroectoderm is noted at the level of the cephalic folds and of the neural tube
• wavy neural tube (MGI Ref ID J:12622)
  • at E9.5, homozygotes exhibit a wavy and disorganized neural tube
  • in addition, the neural tube is often curved and infiltrated with pyknotic cells
• growth/size phenotype
• reduced embryo size (MGI Ref ID J:12622)
  • at E9.5, most homozygous mutant embryos are ~25% to ~50% the size of wild-type embryos
• cardiovascular system phenotype
• poor circulation (MGI Ref ID J:12622)
  • at E9.5, blood circulation is essentially absent in the visceral yolk sac and in mutant embryos
  • however, all homozygotes at this stage show normal decidual sizes and normal beating of the heart
• craniofacial phenotype
• megacephaly (MGI Ref ID J:12622)
  • at E9.5, homozygotes display a disproportionately bulky and dilated head

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Csk^tm1Sor related

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Genes Regulating Growth and Proliferation

Developmental Biology Research
Embryonic Lethality (Homozygous)
Neural Tube Defects
Postnatal Mortality

Neurobiology Research
Neural Tube Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Csktm1Sor
Allele Name		targeted mutation 1, Philippe Soriano
Allele Type		Targeted (knock-out)
Common Name(s)		Csk-;
Mutation Made By		Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin		129S7/SvEvBrd-Hprt1+
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name		Csk, c-src tyrosine kinase
Chromosome		9
Gene Common Name(s)		AW212630; MGC112926; MGC117393; expressed sequence AW212630;
Molecular Note		Deletion of all coding sequences and replacement with a neomycin cassette. [MGI Ref ID J:12622]

Genotyping

Genotyping Information

Genotyping Protocols

Csk^tm1Sor, STD PCR, vers. 1
NEOTD (Generic Neo), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Imamoto A; Soriano P. 1993. Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice. Cell 73(6):1117-24. [PubMed: 7685657]  [MGI Ref ID J:12622]

Additional References

Csk^tm1Sor related

Duan LJ; Imamoto A; Fong GH. 2004. Dual roles of the C-terminal Src kinase (Csk) during developmental vascularization. Blood 103(4):1370-2. [PubMed: 14576066]  [MGI Ref ID J:115663]

Thomas SM; Soriano P; Imamoto A. 1995. Specific and redundant roles of Src and Fyn in organizing the cytoskeleton. Nature 376(6537):267-71. [PubMed: 7617039]  [MGI Ref ID J:113005]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.