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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6-Tg(CD8aCD8b)57Scr/J (Changed: 03-DEC-07 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+2p
Generation DefinitionsDonating Investigator Linda Sherman, The Scripps Research Institute Description
Mice transgenic for the human CD8 alpha and beta chains are viable and fertile. T cells from these mice have enhanced capacity to recognize HLA class I molecules. Augmentation of HLA class I recognition is primarily a function of the human CD8a subunit. Ability of these cells to detect human MHC molecules does not interfere with the normal recognition of allogeneic murine class 1.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Lck
019418 B6(Cg)-Tg(Lck-Cd1d1)1Aben/J 002817 B6.129S2-Lcktm1Mak/J 016101 B6.Cg-Il7rtm1Imx Tg(Lck-Il7r)1676Trma/J 013738 B6.Cg-Tg(LCKprBCL2L1)12Sjk/J 012837 B6.Cg-Tg(Lck-cre)3779Nik/J 003802 B6.Cg-Tg(Lck-cre)548Jxm/J 006889 B6.Cg-Tg(Lck-cre)I540Jxm/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 008842 C57BL/6-Tg(Lck-VIPR2)1Ejg/J 003332 C57BL/6-Tg(LckNotch1)9Erob/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005732 NOD.Cg-Tg(Lck-cre)548Jxm/AchJ View Strains carrying other alleles of Lck (12 strains)
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. CD8 Deficiency, Familial (CD8A)
assigned by genotype
Tg(CD8aCD8b)57Scr/?
B6;SJL-Tg(CD8aCD8b)57Scr/J
- immune system phenotype
- abnormal cytotoxic T cell physiology
- expression of human CD8 allows for effector cell function against target cells presenting viral peptide on human HLA-A2 complexes (MGI Ref ID J:92994)
- this cytolytic effector function is primarily carried out by T cells expressing both mouse and human CD8 complexes (MGI Ref ID J:92994)
- the presence of the human CD8 co-receptor does not interfere with the ability of T cells to recognize mouse MHC (MGI Ref ID J:92994)
- abnormal effector T cell morphology
- hematopoietic system phenotype
- abnormal effector T cell morphology
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CD8aCD8b)57Scr | ||
|---|---|---|---|
| Allele Name | transgene insertion 57, The Scripps Research Institute | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Per Peterson, | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | CD8A, CD8a molecule, human | ||
| Expressed Gene | CD8B, CD8b molecule, human | ||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| Molecular Note | The transgene contains the human CD8 alpha and beta chain genes driven by the T cell-specific Lck promoter. CD8alpha-alpha homodimers were expressed on 62% of thymocytes and CD8alpha-beta heterodimers on 14% of thymocytes. Peripheral T cells preferentially expressed the CD8alpha-beta heterodimers at a 4:1 ratio. Human CD8alpha was detected on both CD4+ and CD8+ T cells. [MGI Ref ID J:92994] | ||
References
References provided by MGI
Selected Reference(s)
LaFace DM; Vestberg M; Yang Y; Srivastava R; DiSanto J; Flomenberg N; Brown S; Sherman LA; Peterson PA. 1995. Human CD8 transgene regulation of HLA recognition by murine T cells. J Exp Med 182(5):1315-25. [PubMed: 7595202] [MGI Ref ID J:92994]
Additional References
Tg(CD8aCD8b)57Scr relatedTishon A; LaFace DM; Lewicki H; van Binnendijk RS; Osterhaus A; Oldstone MB. 2000. Transgenic mice expressing human HLA and CD8 molecules generate HLA-restricted measles virus cytotoxic T lymphocytes of the same specificity as humans with natural measles virus infection. Virology 275(2):286-93. [PubMed: 10998329] [MGI Ref ID J:127813]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry T cell-specific expression of the human CD8 molecules was obtained using a transgene expression vector bearing the murine p56lck proximal promoter. The human CD8a and human CD8b transgene constructs were micro-injected separately or coinjected into (C57BL/6 x SJL)F2 embryos to generate lines with differential expression. Transgenic founder mice were backcrossed to C57BL/6 mice. All mice used for these studies were backcrossed a minimum of three times and were homozygous for the H-2b haplotype. The strain is maintained using homozygous matings.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3175.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $4127.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
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